Sebastian Siegner
@sebasiegner.bsky.social
PhD student in the Corn Lab -
Synthetic Lethality and DNA repair 🧬
Synthetic Lethality and DNA repair 🧬
Reposted by Sebastian Siegner
Thrilled to share a new important study from the lab, published today in @nature.com, showing how cohesin mediates efficient replication fork plasticity and stress response, using its loop extrusion activity to promote 3D contacts on replicating DNA:
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Cohesin reshapes replication fork contacts to aid fork slowing and reversal - Nature
Cohesin-mediated loop extrusion limits sister-fork coupling and tethers nearby replication forks under replication stress, promoting fork reversal and slowing fork progression to safeguard genome...
www.nature.com
September 16, 2026 at 4:04 PM
Thrilled to share a new important study from the lab, published today in @nature.com, showing how cohesin mediates efficient replication fork plasticity and stress response, using its loop extrusion activity to promote 3D contacts on replicating DNA:
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Sebastian Siegner
Fucoidans from brown algae and diatoms drive marine carbon sequestration partly because they're hard to break down. Sichert et al. show how synergistic interactions between degraders are critical for their degradation, giving insights into ocean carbon cycling
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Synergistic degradation of fucoidans in the ocean - Nature
Efficient degradation of fucoidans depends on complementary bacterial guilds that cooperatively break backbone and side-chain sugars, revealing a conserved, globally relevant mechanism that shapes mar...
www.nature.com
September 9, 2026 at 1:09 PM
Fucoidans from brown algae and diatoms drive marine carbon sequestration partly because they're hard to break down. Sichert et al. show how synergistic interactions between degraders are critical for their degradation, giving insights into ocean carbon cycling
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Sebastian Siegner
Do you work in #cancer and feel behind-the-curve in #CRISPR state of the art? Get up to speed fast with this review! Huge kudos to Joshua Casan, Samuel Grigg, and Carolyn Shembrey for heavy lifting to pull this together. www.nature.com/articles/s41...
CRISPR in clinical oncology: translational advances from molecular diagnostics to therapeutics - Nature Reviews Clinical Oncology
CRISPR–Cas technologies provide a modular, programmable platform for sequence-specific targeting of DNA or RNA and have advanced from experimental molecular biology tools to clinical translation. This...
www.nature.com
July 14, 2026 at 9:14 AM
Do you work in #cancer and feel behind-the-curve in #CRISPR state of the art? Get up to speed fast with this review! Huge kudos to Joshua Casan, Samuel Grigg, and Carolyn Shembrey for heavy lifting to pull this together. www.nature.com/articles/s41...
Reposted by Sebastian Siegner
Do you need a super active #CRISPR Cas for in vivo editing? I have not one, but two options for you. Activities rival SpyCas9, but they fit into an scAAV. Check out our latest paper, co-led by Fedor Gorbenko and Irene Sala. Just out in Genome Research. link.springer.com/article/10.1...
June 30, 2026 at 10:25 PM
Do you need a super active #CRISPR Cas for in vivo editing? I have not one, but two options for you. Activities rival SpyCas9, but they fit into an scAAV. Check out our latest paper, co-led by Fedor Gorbenko and Irene Sala. Just out in Genome Research. link.springer.com/article/10.1...
Reposted by Sebastian Siegner
Excited to finally share our preprint on mapping the genetic interaction network of the DNA damage response with combinatorial knockout screens led by Sam Hayward, Alina Vaitsiankova, and Tomas Lama-Diaz! www.biorxiv.org/cgi/content/...
Mapping the genetic landscape of the DNA damage response with Cas12a-based combinatorial knockout screens
The DNA damage response (DDR) is a complex network of cellular pathways that ensures the faithful maintenance of our genomes upon a wide array of genomic insults. To elucidate the functional architect...
www.biorxiv.org
June 8, 2026 at 9:49 PM
Excited to finally share our preprint on mapping the genetic interaction network of the DNA damage response with combinatorial knockout screens led by Sam Hayward, Alina Vaitsiankova, and Tomas Lama-Diaz! www.biorxiv.org/cgi/content/...
Reposted by Sebastian Siegner
new lab preprint - Protein function prediction is a well established problem but proteins exist in different post-translationally modified forms. Here, @julianvangerwen.bsky.social built a ML model to predict the biological process regulated by individual phosphosites www.biorxiv.org/content/10.6...
www.biorxiv.org
May 25, 2026 at 8:06 AM
new lab preprint - Protein function prediction is a well established problem but proteins exist in different post-translationally modified forms. Here, @julianvangerwen.bsky.social built a ML model to predict the biological process regulated by individual phosphosites www.biorxiv.org/content/10.6...
Reposted by Sebastian Siegner
How do cancer-associated eccDNAs arise?
Our work shows that single-strand DNA damage in repetitive sequences can trigger i-loop formation and eccDNA generation. What started at telomeres may reflect a much broader principle across the genome and help explain how ecDNA may emerge in cancer. 1/2
Our work shows that single-strand DNA damage in repetitive sequences can trigger i-loop formation and eccDNA generation. What started at telomeres may reflect a much broader principle across the genome and help explain how ecDNA may emerge in cancer. 1/2
April 30, 2026 at 3:17 PM
How do cancer-associated eccDNAs arise?
Our work shows that single-strand DNA damage in repetitive sequences can trigger i-loop formation and eccDNA generation. What started at telomeres may reflect a much broader principle across the genome and help explain how ecDNA may emerge in cancer. 1/2
Our work shows that single-strand DNA damage in repetitive sequences can trigger i-loop formation and eccDNA generation. What started at telomeres may reflect a much broader principle across the genome and help explain how ecDNA may emerge in cancer. 1/2
Reposted by Sebastian Siegner
Proper organelle regulation during mitosis is a must, but how do membrane-less condensates like PML bodies behave during cell division? In work led by Eric Aird, we found the balance of speckled proteins SP110 & SP100 to be the key. doi.org/10.1038/s415...
March 18, 2026 at 11:53 PM
Proper organelle regulation during mitosis is a must, but how do membrane-less condensates like PML bodies behave during cell division? In work led by Eric Aird, we found the balance of speckled proteins SP110 & SP100 to be the key. doi.org/10.1038/s415...
Reposted by Sebastian Siegner
Many genome-editing tools work by cutting DNA - but the shape of the cut matters. Using genome-wide screening, we show ERCC6L2 is specifically required to maintain repair fidelity at staggered DNA breaks, but not at blunt ends.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
March 12, 2026 at 12:55 AM
Many genome-editing tools work by cutting DNA - but the shape of the cut matters. Using genome-wide screening, we show ERCC6L2 is specifically required to maintain repair fidelity at staggered DNA breaks, but not at blunt ends.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Sebastian Siegner
Happy to share our new pre-print on the dependency of ALT-positive tumors on the DNA translocase SMARCAL1. Outstanding work of @angelotaglialatela.bsky.social in collaboration with the Min, Lazzerini Denchi and @cejkalab.bsky.social laboratories! www.biorxiv.org/content/10.6...
www.biorxiv.org
February 17, 2026 at 3:57 AM
Happy to share our new pre-print on the dependency of ALT-positive tumors on the DNA translocase SMARCAL1. Outstanding work of @angelotaglialatela.bsky.social in collaboration with the Min, Lazzerini Denchi and @cejkalab.bsky.social laboratories! www.biorxiv.org/content/10.6...
Reposted by Sebastian Siegner
Very cool work published in @science.org led by OanaPelea,
happy to participate!
www.science.org/doi/10.1126/...
happy to participate!
www.science.org/doi/10.1126/...
a man wearing a beanie and a black jacket is smiling and holding a piece of food .
ALT: a man wearing a beanie and a black jacket is smiling and holding a piece of food .
media.tenor.com
February 5, 2026 at 8:12 PM
Very cool work published in @science.org led by OanaPelea,
happy to participate!
www.science.org/doi/10.1126/...
happy to participate!
www.science.org/doi/10.1126/...
Reposted by Sebastian Siegner
Excited to share our new Nature study! 🧬
We (sidrituruci.bsky.social et al) discovered that CFAP20 helps clear stalled RNAPII, preventing harmful clashes with DNA replication machinery. This protects cells from R-loops and genome instability.
Full paper: www.nature.com/articles/s41...
We (sidrituruci.bsky.social et al) discovered that CFAP20 helps clear stalled RNAPII, preventing harmful clashes with DNA replication machinery. This protects cells from R-loops and genome instability.
Full paper: www.nature.com/articles/s41...
January 15, 2026 at 7:45 AM
Excited to share our new Nature study! 🧬
We (sidrituruci.bsky.social et al) discovered that CFAP20 helps clear stalled RNAPII, preventing harmful clashes with DNA replication machinery. This protects cells from R-loops and genome instability.
Full paper: www.nature.com/articles/s41...
We (sidrituruci.bsky.social et al) discovered that CFAP20 helps clear stalled RNAPII, preventing harmful clashes with DNA replication machinery. This protects cells from R-loops and genome instability.
Full paper: www.nature.com/articles/s41...
Reposted by Sebastian Siegner
One more upcoming appointment with great science via the Genome Integrity Italian Network (GiiN). Don't miss it, just join via the link 👇
January 8, 2026 at 3:47 PM
One more upcoming appointment with great science via the Genome Integrity Italian Network (GiiN). Don't miss it, just join via the link 👇
Reposted by Sebastian Siegner
New research group leader @matthiasmuhar.bsky.social joins @mpi-cbg.de! 🥳 With his group "Functional genomics of proteome remodeling,” Matthias wants to pursue high-throughput genetic studies to understand how protein turnover is regulated. Welcome, Matthias! www.mpi-cbg.de/news-outreac...
New research group leader for functional genomics
Molecular biologist Matthias Muhar becomes part of the MPI-CBG faculty
www.mpi-cbg.de
December 11, 2025 at 2:15 PM
New research group leader @matthiasmuhar.bsky.social joins @mpi-cbg.de! 🥳 With his group "Functional genomics of proteome remodeling,” Matthias wants to pursue high-throughput genetic studies to understand how protein turnover is regulated. Welcome, Matthias! www.mpi-cbg.de/news-outreac...
Reposted by Sebastian Siegner
Congrats to Moritz Schlapansky, who successfully defended his PhD thesis on Friday! You can read his work on "scOUT-seq" profiling of single cell transcriptomes + editing outcomes in millions of cells and living mice at www.biorxiv.org/content/10.1...
November 10, 2025 at 7:56 AM
Congrats to Moritz Schlapansky, who successfully defended his PhD thesis on Friday! You can read his work on "scOUT-seq" profiling of single cell transcriptomes + editing outcomes in millions of cells and living mice at www.biorxiv.org/content/10.1...
Reposted by Sebastian Siegner
Sometimes #Cas9 is too dang big. But mini Cas proteins are bad at both NHEJ & HDR. PhD student Fedor Gorbenko used mammalian cell evolution for *HDR* to make REALLY super #Cas12f1 and #TnpB. As good as or better than Cas9! Base editors included! www.biorxiv.org/cgi/content/short/2025.10.27.684765v1
Directed evolution of compact RNA-guided nucleases for homology-directed repair in mammalian cells
RNA-guided nucleases enable DNA editing and offer promise for treating genetic diseases, particularly when used for precise sequence replacement. However, many of the most effective enzymes, such as S...
www.biorxiv.org
October 28, 2025 at 1:28 PM
Sometimes #Cas9 is too dang big. But mini Cas proteins are bad at both NHEJ & HDR. PhD student Fedor Gorbenko used mammalian cell evolution for *HDR* to make REALLY super #Cas12f1 and #TnpB. As good as or better than Cas9! Base editors included! www.biorxiv.org/cgi/content/short/2025.10.27.684765v1
Reposted by Sebastian Siegner
Thanks to CRISPR/Cas technology, researchers can precisely edit genetic material to treat hereditary diseases. To achieve this, they need to identify undesirable cuts in the genome at an early juncture. Pioneer Fellow Lilly van de Venn is developing such testing methods. ethz.ch/en/news-and-...
An ETH spin-off aims to bring gene scissors to the clinic
Thanks to CRISPR/Cas technology, researchers can precisely edit genetic material to treat hereditary diseases. To achieve this, they need to identify undesirable cuts in the genome at an early junctur...
ethz.ch
October 27, 2025 at 10:08 AM
Thanks to CRISPR/Cas technology, researchers can precisely edit genetic material to treat hereditary diseases. To achieve this, they need to identify undesirable cuts in the genome at an early juncture. Pioneer Fellow Lilly van de Venn is developing such testing methods. ethz.ch/en/news-and-...
Reposted by Sebastian Siegner
Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...
Cell-stereotyped DNA repair outcomes are widespread during genome editing
Genome editing outcomes are governed by DNA repair pathways that vary with cell type and state. We developed scOUT-seq (single-cell Outcomes Using Transcript sequencing), a scalable approach that join...
www.biorxiv.org
October 24, 2025 at 8:44 AM
Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...
Reposted by Sebastian Siegner
On that note: hello world! My new lab on genetics of proteome remodeling is starting at the incredible MPI-CBG. We have big plans and open positions for PhD students and an RA. Apply now (!) at imprs.mpi-cbg.de and tinyurl.com/2vywj6f6 respectively.
October 21, 2025 at 9:53 PM
On that note: hello world! My new lab on genetics of proteome remodeling is starting at the incredible MPI-CBG. We have big plans and open positions for PhD students and an RA. Apply now (!) at imprs.mpi-cbg.de and tinyurl.com/2vywj6f6 respectively.
Reposted by Sebastian Siegner
If you are interested in a PhD in the area of "Mechanisms of Genome Stability" and "Functional Genomics of Proteome Remodelling," apply now for a PhD @mpi-cbg.de! Overview of all participating RGLs: imprs.mpi-cbg.de/5761/researc...
October 20, 2025 at 1:36 PM
If you are interested in a PhD in the area of "Mechanisms of Genome Stability" and "Functional Genomics of Proteome Remodelling," apply now for a PhD @mpi-cbg.de! Overview of all participating RGLs: imprs.mpi-cbg.de/5761/researc...
Reposted by Sebastian Siegner
Just out in @science.org: Together with the lab of @wilhelmpalm.bsky.social, we used sequential in-vitro/in-vivo CRISPR screens to decipher metabolic adaptations in tumors. We find that acidosis is a dominant factor that shapes energy metabolism and stress resilience. www.science.org/doi/10.1126/...
October 9, 2025 at 7:37 PM
Just out in @science.org: Together with the lab of @wilhelmpalm.bsky.social, we used sequential in-vitro/in-vivo CRISPR screens to decipher metabolic adaptations in tumors. We find that acidosis is a dominant factor that shapes energy metabolism and stress resilience. www.science.org/doi/10.1126/...
Reposted by Sebastian Siegner
Happy to share our latest research on the BRCA1-BRCTs now online.
academic.oup.com/nar/article/...
Congrats to first author Venda for putting it all together! This project was a great collaboration with the @titiasixma.bsky.social lab who helped us give structural meaning to our observations.
academic.oup.com/nar/article/...
Congrats to first author Venda for putting it all together! This project was a great collaboration with the @titiasixma.bsky.social lab who helped us give structural meaning to our observations.
Validate User
academic.oup.com
September 18, 2025 at 8:40 AM
Happy to share our latest research on the BRCA1-BRCTs now online.
academic.oup.com/nar/article/...
Congrats to first author Venda for putting it all together! This project was a great collaboration with the @titiasixma.bsky.social lab who helped us give structural meaning to our observations.
academic.oup.com/nar/article/...
Congrats to first author Venda for putting it all together! This project was a great collaboration with the @titiasixma.bsky.social lab who helped us give structural meaning to our observations.
Reposted by Sebastian Siegner
New work from the lab! 🔬
We report that DDIAS is a new component of the mitotic CIP2A-TOPBP1 pathway of genome maintenance. 1/n
www.biorxiv.org/content/10.1...
We report that DDIAS is a new component of the mitotic CIP2A-TOPBP1 pathway of genome maintenance. 1/n
www.biorxiv.org/content/10.1...
Mitotic single-stranded DNA suppression by DDIAS
Incomplete DNA replication and chromosome breakage during mitosis pose major threats to chromosome segregation. The CIP2A-TOPBP1 complex acts to mitigate this peril, but its exact role is not yet unde...
www.biorxiv.org
September 10, 2025 at 4:50 PM
New work from the lab! 🔬
We report that DDIAS is a new component of the mitotic CIP2A-TOPBP1 pathway of genome maintenance. 1/n
www.biorxiv.org/content/10.1...
We report that DDIAS is a new component of the mitotic CIP2A-TOPBP1 pathway of genome maintenance. 1/n
www.biorxiv.org/content/10.1...
Reposted by Sebastian Siegner
JOB ALERT 🚨 We are hiring TWO principal investigators in cell, molecular, systems, or chemical biology in Toronto, Canada at @sinaihealth.bsky.social. We provide a generous startup, fully funded salary and academic appointment at U of Toronto.
www.nature.com/naturecareer...
Please repost!
www.nature.com/naturecareer...
Please repost!
August 28, 2025 at 6:34 AM
JOB ALERT 🚨 We are hiring TWO principal investigators in cell, molecular, systems, or chemical biology in Toronto, Canada at @sinaihealth.bsky.social. We provide a generous startup, fully funded salary and academic appointment at U of Toronto.
www.nature.com/naturecareer...
Please repost!
www.nature.com/naturecareer...
Please repost!