Vikram Paralkar
@paralkarlab.bsky.social
Physician-Scientist | University of Pennsylvania | Pol I, rRNA, Ribosomes, Chromatin, Transcription | Hematopoiesis and Leukemia | 🩸🔬🧪🧬
https://paralkarlab.med.upenn.edu
https://paralkarlab.med.upenn.edu
Pinned
Dynamics of Ribosomal RNA Transcription and Abundance in Normal and Leukemic Hematopoiesis
Transcription of ribosomal RNAs (rRNAs) from rDNA repeats is the first step of ribosome biogenesis, accounting for a major portion of all cellular transcription. Often regarded as a housekeeping proce...
www.biorxiv.org
📣 New preprint 📣
We present a detailed map of nascent and mature ribosomal RNAs in mouse hematopoiesis and AML (including absolute rRNA molecule numbers), and outline principles of rDNA regulation.
Work by Eleanor Sams (@eleanor44.bsky.social) & Victoria Feist (@victoriafeist.bsky.social). (1/x)
We present a detailed map of nascent and mature ribosomal RNAs in mouse hematopoiesis and AML (including absolute rRNA molecule numbers), and outline principles of rDNA regulation.
Work by Eleanor Sams (@eleanor44.bsky.social) & Victoria Feist (@victoriafeist.bsky.social). (1/x)
Reposted by Vikram Paralkar
found at ucsf
September 17, 2026 at 1:05 PM
found at ucsf
Reposted by Vikram Paralkar
Everything you wanted to know about single-molecule epigenomics but were too afraid to ask! Wonderful to co-write (h/t ENORMOUS lift by @arnaudkr.bsky.social) this primer on our nascent field. Also, a 💯 example of wonderful colleagues building something *together*, not in competition =)
A practical guide to studying genome function using single-molecule genomics
Nature Reviews Molecular Cell Biology - Single-molecule genomics methods are used to study the activity of regulatory factors on individual DNA molecules genome-wide, thereby enabling...
www.nature.com
September 14, 2026 at 12:33 PM
Everything you wanted to know about single-molecule epigenomics but were too afraid to ask! Wonderful to co-write (h/t ENORMOUS lift by @arnaudkr.bsky.social) this primer on our nascent field. Also, a 💯 example of wonderful colleagues building something *together*, not in competition =)
Reposted by Vikram Paralkar
James Bond: you think I haven’t faced death before?
Supervillain: oh, I don’t expect you to die, Mr. Bond. I expect you to suffer.
*opens travel funding reimbursement software
I think you will find your motorboat rental and casino reservations especially complex
James Bond: no. no. pls kill me
Supervillain: oh, I don’t expect you to die, Mr. Bond. I expect you to suffer.
*opens travel funding reimbursement software
I think you will find your motorboat rental and casino reservations especially complex
James Bond: no. no. pls kill me
August 21, 2026 at 2:19 AM
James Bond: you think I haven’t faced death before?
Supervillain: oh, I don’t expect you to die, Mr. Bond. I expect you to suffer.
*opens travel funding reimbursement software
I think you will find your motorboat rental and casino reservations especially complex
James Bond: no. no. pls kill me
Supervillain: oh, I don’t expect you to die, Mr. Bond. I expect you to suffer.
*opens travel funding reimbursement software
I think you will find your motorboat rental and casino reservations especially complex
James Bond: no. no. pls kill me
Reposted by Vikram Paralkar
🚨👇 Check out this wonderful collaborative work identifying a new 3D genome regulator - Matchmakers - with the incredible @andersshansen.bsky.social ! Fantastic work led by Varshini Ramanathan and Chun-Jie Guo!
(1/n) Excited to share close collab w @bloodgenes.bsky.social led by Varshini & Chun-jie et al
How to induce expression of key genes while silencing much of the genome during Erythropoiesis?
A: Matchmaker CREs load cohesin near key genes to promote looping & exp:
www.biorxiv.org/content/10.6...
How to induce expression of key genes while silencing much of the genome during Erythropoiesis?
A: Matchmaker CREs load cohesin near key genes to promote looping & exp:
www.biorxiv.org/content/10.6...
August 6, 2026 at 6:59 PM
🚨👇 Check out this wonderful collaborative work identifying a new 3D genome regulator - Matchmakers - with the incredible @andersshansen.bsky.social ! Fantastic work led by Varshini Ramanathan and Chun-Jie Guo!
Reposted by Vikram Paralkar
For the past 30 years, “whole-genome sequencing” has been a misnomer. Today the T2T Consortium publishes a dozen papers heralding a future of truly complete genomes for humans and nearly any vertebrate 👨🔬🐒🐦🐀🦒🐎🫏🐹🐟 (sorry, no salamanders): www.cell.com/consortium/t... 🧵[1/15]
August 6, 2026 at 3:42 PM
For the past 30 years, “whole-genome sequencing” has been a misnomer. Today the T2T Consortium publishes a dozen papers heralding a future of truly complete genomes for humans and nearly any vertebrate 👨🔬🐒🐦🐀🦒🐎🫏🐹🐟 (sorry, no salamanders): www.cell.com/consortium/t... 🧵[1/15]
Reposted by Vikram Paralkar
RNA-seq tells us how much RNA is present in the cell. But to understand gene regulation, we need to easily measure the synthesis and decay rates driving this abundance. We introduce AIR-seq: analog intrinsic recoding sequencing. (1/6) www.biorxiv.org/content/10.6...
Analog intrinsic recoding measures RNA dynamics without chemical conversion
Steady-state RNA abundance measurements mask the synthesis and decay rates that shape gene expression. Analog intrinsic recoding sequencing (AIR-seq) repurposes the base-pairing properties of N4-hydro...
www.biorxiv.org
July 28, 2026 at 5:20 PM
RNA-seq tells us how much RNA is present in the cell. But to understand gene regulation, we need to easily measure the synthesis and decay rates driving this abundance. We introduce AIR-seq: analog intrinsic recoding sequencing. (1/6) www.biorxiv.org/content/10.6...
Except the malignant ones! 😱
In case you need a little pick-me-up to start your week, courtesy of Fred Hutch’s Human Biology Division. 🔬🧫🤗
July 27, 2026 at 6:09 PM
Except the malignant ones! 😱
Reposted by Vikram Paralkar
the most realistic scene in Jurassic Park is when two scientists make a questionable decision — traveling to an undisclosed location with a strange man — on the promise of three years of modest funding
July 21, 2026 at 12:43 AM
the most realistic scene in Jurassic Park is when two scientists make a questionable decision — traveling to an undisclosed location with a strange man — on the promise of three years of modest funding
Reposted by Vikram Paralkar
THE MYELOID WORKSHOP IS ON! We listened to your feedback and packed more talks in; will start 1 hr earlier. The ASH website will list workshops soon, stay tuned for how to request speaking slots @steidluli.bsky.social
July 19, 2026 at 4:40 PM
THE MYELOID WORKSHOP IS ON! We listened to your feedback and packed more talks in; will start 1 hr earlier. The ASH website will list workshops soon, stay tuned for how to request speaking slots @steidluli.bsky.social
Reposted by Vikram Paralkar
Could Excel be conscious? We surveyed more than Jan 1 1900 people to find out.
July 19, 2026 at 4:27 PM
Could Excel be conscious? We surveyed more than Jan 1 1900 people to find out.
The word "honorific" and "horrific" are too close together. Who designed this language?
July 16, 2026 at 3:03 PM
The word "honorific" and "horrific" are too close together. Who designed this language?
Reposted by Vikram Paralkar
Excited to share our latest preprint on AML, now on bioRxiv - biorxiv.org/content/10.6... A🧵
Disrupting a Convergent Acetylation Circuit Collapses Leukemic Identity Across AML Subtypes
Acute myeloid leukemia (AML) cells rely on an actively maintained, chromatin-encoded transcriptional program to sustain their malignant identity, offering a potential therapeutic vulnerability. Using ...
biorxiv.org
July 14, 2026 at 1:59 PM
Excited to share our latest preprint on AML, now on bioRxiv - biorxiv.org/content/10.6... A🧵
I'm a fan of Post Malone's early music. When he was Pre Malone.
July 5, 2026 at 6:09 PM
I'm a fan of Post Malone's early music. When he was Pre Malone.
Reposted by Vikram Paralkar
🚨🚨NEW PREPRINT ALERT!🚨🚨Proud to present our recent study, out now on bioRxiv, in which we reveal an RNA-dependent positive feedback loop that drives malignant ribosome biogenesis and tumour growth (1/15).
🔗
www.biorxiv.org/content/10.6...
🔗
www.biorxiv.org/content/10.6...
July 3, 2026 at 7:18 PM
🚨🚨NEW PREPRINT ALERT!🚨🚨Proud to present our recent study, out now on bioRxiv, in which we reveal an RNA-dependent positive feedback loop that drives malignant ribosome biogenesis and tumour growth (1/15).
🔗
www.biorxiv.org/content/10.6...
🔗
www.biorxiv.org/content/10.6...
Reposted by Vikram Paralkar
I am excited and proud to be an organizer of this fantastic meeting. I always walk away with new friends and colleagues and having learned so much. Hope to see you there!
The abstract submission deadline for #EESRNA is only a week away!
Don't miss your chance to present your research at the EMBO | EMBL Symposium 'The complex life of RNA', the central event for the RNA community. Submit your abstract by 7 July: s.embl.org/ees26-12-bl
#RNA #RNAbiology
Don't miss your chance to present your research at the EMBO | EMBL Symposium 'The complex life of RNA', the central event for the RNA community. Submit your abstract by 7 July: s.embl.org/ees26-12-bl
#RNA #RNAbiology
July 1, 2026 at 12:38 PM
I am excited and proud to be an organizer of this fantastic meeting. I always walk away with new friends and colleagues and having learned so much. Hope to see you there!
Reposted by Vikram Paralkar
A deeper dive into the article we published last week in @nature.com.
I will start with the framing and methodology as they are key to understanding the surprising results:
Most proteomics experiments begin with an assumption.
www.nature.com/articles/s41...
1/
I will start with the framing and methodology as they are key to understanding the surprising results:
Most proteomics experiments begin with an assumption.
www.nature.com/articles/s41...
1/
June 30, 2026 at 12:28 PM
A deeper dive into the article we published last week in @nature.com.
I will start with the framing and methodology as they are key to understanding the surprising results:
Most proteomics experiments begin with an assumption.
www.nature.com/articles/s41...
1/
I will start with the framing and methodology as they are key to understanding the surprising results:
Most proteomics experiments begin with an assumption.
www.nature.com/articles/s41...
1/
What I like about preprints: They don't come with any baked-in impact-factor-like imprimatur that elevates some over others.
What would be valuable: An AI-based system that learns a user's scientific questions, and converts the firehose of preprints into a gentle, personally-curated stream.
What would be valuable: An AI-based system that learns a user's scientific questions, and converts the firehose of preprints into a gentle, personally-curated stream.
June 29, 2026 at 5:46 PM
What I like about preprints: They don't come with any baked-in impact-factor-like imprimatur that elevates some over others.
What would be valuable: An AI-based system that learns a user's scientific questions, and converts the firehose of preprints into a gentle, personally-curated stream.
What would be valuable: An AI-based system that learns a user's scientific questions, and converts the firehose of preprints into a gentle, personally-curated stream.
Biting satire about our increasing reliance on LLMs! 😳
Opinion: I Was Not Allowed To Type Prompts Into ChatGPT During My Chalk Talk And This Is Discrimination
By Dr. Rachel Simmons, Postdoctoral Fellow, Stanford University
inpreparation.substack.com
June 23, 2026 at 12:11 PM
Biting satire about our increasing reliance on LLMs! 😳
Reposted by Vikram Paralkar
(1/n) Very excited to share tri-lab collab (Mirny & Zechner) led by Harvey, Henrik & Jack:
Q: How do enhancers & promoters interact in space (contact vs. action-at-a-distance) and time (stable vs. transient)?
A: Transient E-P contact (~25-42 nm lasting ~10-20 sec):
www.biorxiv.org/content/10.6...
Q: How do enhancers & promoters interact in space (contact vs. action-at-a-distance) and time (stable vs. transient)?
A: Transient E-P contact (~25-42 nm lasting ~10-20 sec):
www.biorxiv.org/content/10.6...
June 22, 2026 at 3:32 PM
(1/n) Very excited to share tri-lab collab (Mirny & Zechner) led by Harvey, Henrik & Jack:
Q: How do enhancers & promoters interact in space (contact vs. action-at-a-distance) and time (stable vs. transient)?
A: Transient E-P contact (~25-42 nm lasting ~10-20 sec):
www.biorxiv.org/content/10.6...
Q: How do enhancers & promoters interact in space (contact vs. action-at-a-distance) and time (stable vs. transient)?
A: Transient E-P contact (~25-42 nm lasting ~10-20 sec):
www.biorxiv.org/content/10.6...
Reposted by Vikram Paralkar
My nine year old asked for a Rosetta Stone cake for his birthday
June 21, 2026 at 3:04 PM
My nine year old asked for a Rosetta Stone cake for his birthday
Reposted by Vikram Paralkar
The 2026 Rousseeuw Prize for Statistics has been awarded to the R Project. #RStats
www.rousseeuwprize.org/2026
www.rousseeuwprize.org/2026
The Rousseeuw Prize for Statistics
The Rousseeuw Prize for Statistics is a biennial prize to celebrate outstanding contributions to statistics research.
www.rousseeuwprize.org
June 17, 2026 at 6:44 AM
The 2026 Rousseeuw Prize for Statistics has been awarded to the R Project. #RStats
www.rousseeuwprize.org/2026
www.rousseeuwprize.org/2026
Reposted by Vikram Paralkar
I am deeply honoured to be a laureate of the 2026 Rousseeuw Prize, which has been awarded to the R Project.
I have been on the R Core Team for almost 30 years, We built and maintained R with a vision to make statistical tools freely available and extendable.
Congratulations to entire team!
I have been on the R Core Team for almost 30 years, We built and maintained R with a vision to make statistical tools freely available and extendable.
Congratulations to entire team!
The 2026 Rousseeuw Prize for Statistics has been awarded to the R Project. #RStats
www.rousseeuwprize.org/2026
www.rousseeuwprize.org/2026
The Rousseeuw Prize for Statistics
The Rousseeuw Prize for Statistics is a biennial prize to celebrate outstanding contributions to statistics research.
www.rousseeuwprize.org
June 17, 2026 at 9:48 AM
I am deeply honoured to be a laureate of the 2026 Rousseeuw Prize, which has been awarded to the R Project.
I have been on the R Core Team for almost 30 years, We built and maintained R with a vision to make statistical tools freely available and extendable.
Congratulations to entire team!
I have been on the R Core Team for almost 30 years, We built and maintained R with a vision to make statistical tools freely available and extendable.
Congratulations to entire team!
Had a nightmare that I triple-booked inpatient clinical service with a university lecture and a PhD thesis committee defense, and became aware of the conflicts the night before!
Now I'm awake and relieved this wasn't real, but have a fresh new anxiety that I might do this some day! 😅
Now I'm awake and relieved this wasn't real, but have a fresh new anxiety that I might do this some day! 😅
June 6, 2026 at 11:10 AM
Had a nightmare that I triple-booked inpatient clinical service with a university lecture and a PhD thesis committee defense, and became aware of the conflicts the night before!
Now I'm awake and relieved this wasn't real, but have a fresh new anxiety that I might do this some day! 😅
Now I'm awake and relieved this wasn't real, but have a fresh new anxiety that I might do this some day! 😅
Reposted by Vikram Paralkar
1/3 Delighted to have visited the University of Pennsylvania this week, hosted by the fantastic Dr. Bobby Bowman who has built an exciting research program on the origins of myeloid malignancies and leukemia relapse.
June 3, 2026 at 6:19 PM
1/3 Delighted to have visited the University of Pennsylvania this week, hosted by the fantastic Dr. Bobby Bowman who has built an exciting research program on the origins of myeloid malignancies and leukemia relapse.
Reposted by Vikram Paralkar
🧵 NEW PAPER in @bloodjournal.bsky.social!
Why do IDH1 mutations almost exclusively drive myeloid #leukemia, while IDH2 mutations occur in both myeloid AND lymphoid cancers? 🤔 #AML #Epigenetics
Despite sharing the same biochemical mechanism, they are NOT equivalent. Here's what we found 👇
Why do IDH1 mutations almost exclusively drive myeloid #leukemia, while IDH2 mutations occur in both myeloid AND lymphoid cancers? 🤔 #AML #Epigenetics
Despite sharing the same biochemical mechanism, they are NOT equivalent. Here's what we found 👇
May 29, 2026 at 4:33 PM
🧵 NEW PAPER in @bloodjournal.bsky.social!
Why do IDH1 mutations almost exclusively drive myeloid #leukemia, while IDH2 mutations occur in both myeloid AND lymphoid cancers? 🤔 #AML #Epigenetics
Despite sharing the same biochemical mechanism, they are NOT equivalent. Here's what we found 👇
Why do IDH1 mutations almost exclusively drive myeloid #leukemia, while IDH2 mutations occur in both myeloid AND lymphoid cancers? 🤔 #AML #Epigenetics
Despite sharing the same biochemical mechanism, they are NOT equivalent. Here's what we found 👇