Asher King
@subtisilly.bsky.social
Bacillus subtilis fanatic / PhD candidate in the Eswara Lab at USF ☀️
Reposted by Asher King
the "Mg²⁺ problem" has been known for a long time. For example, in the standard "rich medium" LB (see👇).
now we get to know more about the reasons! #MicroSky
now we get to know more about the reasons! #MicroSky
September 18, 2026 at 8:34 PM
the "Mg²⁺ problem" has been known for a long time. For example, in the standard "rich medium" LB (see👇).
now we get to know more about the reasons! #MicroSky
now we get to know more about the reasons! #MicroSky
Reposted by Asher King
Folks have known for years that adding magnesium to media rescues the lethality of deleting many 'essential' genes, but why? @eswaralab.bsky.social and @subtisilly.bsky.social say that free iron builds up in these mutants. Mg2+ mitigates this by redirecting metabolism towards limiting ROS production
I am so excited to share my first first-author manuscript regarding the mysterious effects of magnesium in Bacillus subtilis! 🧵⬇️
www.biorxiv.org/content/10.6...
#MicroSky
www.biorxiv.org/content/10.6...
#MicroSky
Magnesium induces iron starvation and metabolic rewiring to support the viability of cell envelope mutants and antibiotic-stressed cells
Magnesium supplementation permits deletion of otherwise essential genes involved in cell envelope biogenesis in the Gram-positive model bacterium Bacillus subtilis. Yet, the specific underlying mechan...
www.biorxiv.org
September 18, 2026 at 12:03 PM
Folks have known for years that adding magnesium to media rescues the lethality of deleting many 'essential' genes, but why? @eswaralab.bsky.social and @subtisilly.bsky.social say that free iron builds up in these mutants. Mg2+ mitigates this by redirecting metabolism towards limiting ROS production
Reposted by Asher King
Thrilled to share this insightful work of Asher King @subtisilly.bsky.social and Pilar Horigian @pilarhorigian.bsky.social!
#ProudPI #MicroSky #AMR #SubtiWiki
In this work, we reveal the *magic* behind the protective effects of magnesium in Bacillus subtilis. Read Asher's 🧵 below for more details.
#ProudPI #MicroSky #AMR #SubtiWiki
In this work, we reveal the *magic* behind the protective effects of magnesium in Bacillus subtilis. Read Asher's 🧵 below for more details.
September 2, 2026 at 6:28 PM
Thrilled to share this insightful work of Asher King @subtisilly.bsky.social and Pilar Horigian @pilarhorigian.bsky.social!
#ProudPI #MicroSky #AMR #SubtiWiki
In this work, we reveal the *magic* behind the protective effects of magnesium in Bacillus subtilis. Read Asher's 🧵 below for more details.
#ProudPI #MicroSky #AMR #SubtiWiki
In this work, we reveal the *magic* behind the protective effects of magnesium in Bacillus subtilis. Read Asher's 🧵 below for more details.
Reposted by Asher King
Magnesium induces iron starvation and metabolic rewiring to support the viability of cell envelope mutants and antibiotic-stressed cells https://www.biorxiv.org/content/10.64898/2026.08.31.748404v1
September 2, 2026 at 3:17 AM
Magnesium induces iron starvation and metabolic rewiring to support the viability of cell envelope mutants and antibiotic-stressed cells https://www.biorxiv.org/content/10.64898/2026.08.31.748404v1
I am so excited to share my first first-author manuscript regarding the mysterious effects of magnesium in Bacillus subtilis! 🧵⬇️
www.biorxiv.org/content/10.6...
#MicroSky
www.biorxiv.org/content/10.6...
#MicroSky
Magnesium induces iron starvation and metabolic rewiring to support the viability of cell envelope mutants and antibiotic-stressed cells
Magnesium supplementation permits deletion of otherwise essential genes involved in cell envelope biogenesis in the Gram-positive model bacterium Bacillus subtilis. Yet, the specific underlying mechan...
www.biorxiv.org
September 2, 2026 at 5:28 PM
I am so excited to share my first first-author manuscript regarding the mysterious effects of magnesium in Bacillus subtilis! 🧵⬇️
www.biorxiv.org/content/10.6...
#MicroSky
www.biorxiv.org/content/10.6...
#MicroSky