This matters beyond methodology.

Broad benchmarking claims can shape assay development and clinical deployment and ultimately diagnostic decisions for transplant patients. @asnpublications.bsky.social

#KidneyTransplant #MolecularDiagnostics #Reproducibility

That reframing does not resolve the key concerns: normalization was confounded with deployment strategy and feature selection, and housekeeping-gene stability across rejection phenotypes was not validated.