El colesterol suele tener mala fama porque niveles elevados en sangre se asocian con un mayor riesgo cardiovascular. Sin embargo, esta molécula desempeña funciones esenciales para la vida. Entre otras...

Una plataforma de nanovesículas sintéticas potencia la respuesta inmunitaria frente a distintos patógenos

Un macroestudio que analiza a más de un millón de personas concluye que la influencia del ambiente familiar en el desarrollo de la curiosidad o la amabilidad es menor de lo que se pensaba

#WomeninSTEM - Ada E. Yonath y la estructura del ribosoma

Long RSH enzymes, Rel and RelA, are master regulators of bacterial (p)ppGpp alarmones levels. Bifunctional Rel transitions between a compact hydrolysis-competent (HDON) state, a relaxed catalytically inactive (HDOFF/SYNTHOFF) state, and an elongated synthesis-competent (SYNTHON) state, whereas RelA samples only the latter two. The distribution of these states is controlled by starved ribosomes and regulatory proteins, including DarB, EIIANtr, ACP, NirD and YtfK. Here, we identify and characterize camelid nanobodies that act as selective allosteric modulators by stabilizing Rel and RelA in defined conformational states. Nanobodies that sequester the TGS domain of RelA prevent activation by deacylated tRNA on starved ribosomes, strongly inhibiting (p)ppGpp synthesis and suppressing Escherichia coli virulence in an animal model. Nb898 stabilizes Rel in the open SYNTHON state, enhancing synthesis while suppressing hydrolysis, whereas Nb585 traps Rel in a hydrolysis-competent HDON/SYNTHOFF

Solé-Domènech et al. present acid pH indicator dye (ApHID), a green-emitting pH sensor with optimal fluorescence dynamic range matching the acidity of endolysosomal compartments. ApHID reports pH stably and detects subtle variations in endolysosomal acidification in real time. ApHID outperforms most commercial sensors and can be useful in demanding applications including intravital tissue imaging.

If these podcasts really wanted to nurture enthusiasm for science, they should celebrate the hard work that goes into finding the right answer.

El exdirector de la Agencia para la Calidad Científica y Universitaria de Andalucía (ACCUA), la que que ha vetado que la universidades públicas de Granada

Connections between nerve cells are highly damaged in Alzheimer’s and Parkinson’s disease. This is believed to be linked to the formation of small-soluble aggregates of disease-related proteins. Here,...