The flexible loop in Methanococcus jannaschii dihydroorotase with substrate bound adopts both the loop-out and loop-in conformations #Dihydroorotase #MethanococcusJannaschii #PyrimidineBiosynthesis doi.org/10.1107/S205...
February 12, 2026 at 4:52 PM
The flexible loop in Methanococcus jannaschii dihydroorotase with substrate bound adopts both the loop-out and loop-in conformations #Dihydroorotase #MethanococcusJannaschii #PyrimidineBiosynthesis doi.org/10.1107/S205...
Jacqueline Vitali et al.: Crystal structure of Methanococcus jannaschii dihydroorotase with substrate bound ##Dihydroorotase##MethanococcusJannaschii##PyrimidineBiosynthesis@CLE_State@sunyoldwestbury...##IUCrhttps://journals.iucr.org/paper?S2053230X25010556
Crystal structure of Methanococcus jannaschii dihydroorotase with substrate bound
We present the 1.87 Å resolution structure of M. jannaschii dihydroorotase complexed with the substrate carbamoyl aspartate at room temperature. Contrary to expectations, a flexible loop which is important in catalysis and closes upon substrate binding in order to stabilize the substrate and transition state is observed in both the loop-out and loop-in conformations, with the loop-out conformation having higher occupancy.
journals.iucr.org
December 9, 2025 at 1:00 AM
Jacqueline Vitali et al.: Crystal structure of Methanococcus jannaschii dihydroorotase with substrate bound ##Dihydroorotase##MethanococcusJannaschii##PyrimidineBiosynthesis@CLE_State@sunyoldwestbury...##IUCrhttps://journals.iucr.org/paper?S2053230X25010556