An algorithm is described to recover super-temporal resolution dynamics of X-ray scattering profiles of photo-excited biomolecules in solution from pump–probe X-ray solution scattering data.

A novel validation tool for transmission electron-microscopy maps utilizing independent small-angle X-ray scattering measurements is introduced and implemented. The power of this technique was demonstrated by testing it using simulated data and using it on real experimental data from online repositories.

We present a new analysis approach for calculating the solution X-ray scattering of high-resolution models of biomacromolecules. We introduce novel grid-based methods for evaluating both the hydration shell and excluded volume scattering contributions. The methods are implemented in the new open-source software AUSAXS.

Using scanning X-ray microdiffraction, we discovered that the small-angle scattering from human brain tissue is due largely to the presence of sub-micrometre-sized voids formed during dehydration of the fixed tissue, while the intensity at wide angles derives from the macromolecular material surrounding the voids. The ability to detect and map the presence of voids within thin sections of fixed tissue has the potential to provide novel information on the degradation of human brain tissue in neurodegenerative disease.

Small-angle X-ray scattering-based modelling of various detergents around a multi-hydro­phobic substrate transporter correlates a variation in the shape of the detergent corona with the detergent tail length.