I am generally perform multiple rounds of 2D classification before going into 3D. I like to understand what brings others to start classifying in 3D so early. Can you comment on how the first round of hete refinement looks like? You run abinitio before? How do you select the decoy vols?
I am generally perform multiple rounds of 2D classification before going into 3D. I like to understand what brings others to start classifying in 3D so early. Can you comment on how the first round of hete refinement looks like? You run abinitio before? How do you select the decoy vols?