Abstract. Methane production by a biogas digester is a promising alternative to the use of fossil fuels as an energy source. One major substrate for methan

Abstract Analysis of microbial community composition from five hot springs of the Uzon Caldera (Kamchatka, Russia) using molecular and classical microbiological methods revealed that these thermal eco...

Acetogens are key biocatalysts for carbon-neutral biorefineries, yet their metabolic engineering is limited by the lack of tunable transcriptional regulation systems. Here, we developed a synthetic...

Moorella thermoacetica is the model organism for elucidation of acetogenesis. The reduction of CO2 to acetate is well studied, but how redox carrier balancing and energy conservation are maintained d....

Gear-obsessed editors choose every product we review. We may earn commission if you buy from a link. Why Trust Us? For life to take hold on Earth, it needed a suite of chemical compounds, or seed sets, to create the first methanogens and acetogens. A new study analyzes the capability of asteroids and meteorites to possibly deliver these essential chemicals to Earth’s ancient surface. They found that asteroids like Bennu and carbonaceous chondrite meteorites like Murchison could’ve been, at the very least, one source of these chemicals in Earth’s early history. One of the biggest questions in science is how life formed on Earth, and the pursuit of an answer to this question has inspired many theories—both scientific and theological. For science’s part, it’s all but certain that some key chemical compounds needed to be present for important biological functions to happen, such as the production of adenosine triphosphate (ATP) or DNA. Some theories suggest that this life-making process took place exclusively on Earth, likely catalyzed by ancient volcanic lightning . But other theories suggest that outer space provided a major helping hand in the form of asteroids teaming with microbial life that smashed into Earth, kickstarting a 4.2 billion-year-long...

This work presents the physiological exploration of the halo-alkaliphilic, carboxydotrophic acetogen bacterium MD4. In contrast to well-characterised neutrophilic and slightly acidophilic acetogenic ....

Background Blue Carbon ecosystems, which include all tidal wetlands, mitigate climate change by capturing and storing carbon dioxide (CO2) from the atmosphere. Most carbon fixation in these systems is...

Proteome allocation to anabolic and catabolic functions is significantly regulated by growth rate in the model bacterium Escherichia coli. By contrast, this article shows that proteome allocation is only partially controlled by growth rate, and metabolic rates are primarily controlled post-translationally, in the thermophilic acetogen Thermoanaerobacter kivui.

Syntrophic interaction between acetogens and methanogens is the key to address thermodynamic challenges in anaerobic digestion (AD), the strengthening…

Carbon monoxide (CO) present in syngas severely inhibits growth of many acetogens. Here, the authors adapt, characterize, and engineer utilization of CO as sole carbon and energy source by the thermop...

We present a protocol to produce electrocompetent Acetobacterium woodii DSM 1030 and Eubacterium callanderi DSM 3468 cells. These acetogens are capable of converting CO2 with H2 and other C1 substrates into organic acids. We describe the electroporation procedure and the verification steps to prove the presence of recombinant strains. This protocol serves as a basis for the application of the genetic toolbox for these bacterial species and can be adapted to several anaerobic strains. For complet...