#ActiveZones
LightNews LightNews
plosbiology.org
How do scaffolding proteins regulate the assembly of #presynaptic #ActiveZones? This study solves the structure of the liprin-α2/RIM1 complex & shows that it is essential for #synaptic transmission and LLPS-dependent coupling of Ca2+ channels & synaptic vesicles @plosbiology.org 🧪 plos.io/3Tj5CX7
Top left: Crystal structure of the liprin-α2_CC2N/RIM1_C2B complex. Two neighboring CC2N coiled coils (colored green and gray, respectively), with four bound C2B molecules (colored magenta), are shown. Right inset: Molecular details of interface I formed between the N-terminal part of CC2N and C2B. Salt bridges and hydrogen bonds are indicated by dashed lines. Bottom inset: Molecular details of interface II formed between the C-terminal part of CC2N and C2B. The interconnectivity between interfaces I and II in the complex of one C2B molecule and two CC2N coiled coils is displayed. Salt bridges and hydrogen bonds are indicated by dashed lines. Top right: Surface representation of C2B and CC2N, showing the spatial relationship between the PIP2-binding site and bound CC2N. Key negatively charged residues on the CC2N structure are highlighted in red. Bottom right: Confocal imaging analysis of the LLPS mixture containing the ELKS1/liprin-α2_CC12 (WT or R346E) condensate and the RIM1/RBP2_(SH3)3 condensate. Magnified views of representative RIM1 (a) and ELKS1 (b) droplets were displayed below, with line analyses of fluorescence signal intensities along the indicated lines.
June 11, 2025 at 8:48 AM
plosbiology.org
How do scaffolding proteins regulate the assembly of #presynaptic #ActiveZones? This study solves the structure of the liprin-α2/RIM1 complex & shows that it is essential for #synaptic transmission and LLPS-dependent coupling of Ca2+ channels & synaptic vesicles @plosbiology.org 🧪 plos.io/3Tj5CX7
Top left: Crystal structure of the liprin-α2_CC2N/RIM1_C2B complex. Two neighboring CC2N coiled coils (colored green and gray, respectively), with four bound C2B molecules (colored magenta), are shown. Right inset: Molecular details of interface I formed between the N-terminal part of CC2N and C2B. Salt bridges and hydrogen bonds are indicated by dashed lines. Bottom inset: Molecular details of interface II formed between the C-terminal part of CC2N and C2B. The interconnectivity between interfaces I and II in the complex of one C2B molecule and two CC2N coiled coils is displayed. Salt bridges and hydrogen bonds are indicated by dashed lines. Top right: Surface representation of C2B and CC2N, showing the spatial relationship between the PIP2-binding site and bound CC2N. Key negatively charged residues on the CC2N structure are highlighted in red. Bottom right: Confocal imaging analysis of the LLPS mixture containing the ELKS1/liprin-α2_CC12 (WT or R346E) condensate and the RIM1/RBP2_(SH3)3 condensate. Magnified views of representative RIM1 (a) and ELKS1 (b) droplets were displayed below, with line analyses of fluorescence signal intensities along the indicated lines.
June 11, 2025 at 6:00 PM
plosbiology.org
How do scaffolding proteins regulate the assembly of #presynaptic #ActiveZones? This study solves the structure of the liprin-α2/RIM1 complex & shows that it is essential for #synaptic transmission and LLPS-dependent coupling of Ca2+ channels & synaptic vesicles @plosbiology.org 🧪 plos.io/3Tj5CX7
Top left: Crystal structure of the liprin-α2_CC2N/RIM1_C2B complex. Two neighboring CC2N coiled coils (colored green and gray, respectively), with four bound C2B molecules (colored magenta), are shown. Right inset: Molecular details of interface I formed between the N-terminal part of CC2N and C2B. Salt bridges and hydrogen bonds are indicated by dashed lines. Bottom inset: Molecular details of interface II formed between the C-terminal part of CC2N and C2B. The interconnectivity between interfaces I and II in the complex of one C2B molecule and two CC2N coiled coils is displayed. Salt bridges and hydrogen bonds are indicated by dashed lines. Top right: Surface representation of C2B and CC2N, showing the spatial relationship between the PIP2-binding site and bound CC2N. Key negatively charged residues on the CC2N structure are highlighted in red. Bottom right: Confocal imaging analysis of the LLPS mixture containing the ELKS1/liprin-α2_CC12 (WT or R346E) condensate and the RIM1/RBP2_(SH3)3 condensate. Magnified views of representative RIM1 (a) and ELKS1 (b) droplets were displayed below, with line analyses of fluorescence signal intensities along the indicated lines.
June 11, 2025 at 2:39 PM