#BONCAT
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jcamthrash.bsky.social
Single-cell heterotrophic activity in deep-ocean prokaryotic communities quantified by BONCAT and microautoradiography academic.oup.com/ismecommun/a... #jcampubs 🌊
March 2, 2026 at 5:16 PM
jcamthrash.bsky.social
BONCAT-Live for isolation and cultivation of active environmental bacteria journals.asm.org/doi/full/10.... #jcampubs
September 23, 2025 at 3:22 PM
lynnstorm.bsky.social
Wanna cuddle?
A beautiful comission of my Nightwing Ferrousforger, made by awesome Sunnie BonCat! So much detail and so fast too, definitely worth the money!
@sunnieboncatart.bsky.social
July 23, 2026 at 8:40 AM
cp-trendsplantsci.bsky.social
New opportunities for BONCAT use in plants #plantscience
New opportunities for BONCAT use in plants
Bioorthogonal noncanonical amino acid tagging (BONCAT) bridges the RNA-informed translatome and proteome by isolating newly synthesized proteins. Recent implementation in plants has demonstrated the potential of BONCAT experimentation, which still lags behind its utilization in other fields. In this forum, current and potential future uses of BONCAT in plants are discussed.
dlvr.it
May 15, 2026 at 3:57 PM
uhriglab.bsky.social
!! Check out our new pre-print on using BONCAT Click Chemistry in plants !!

Optimization of Bio-Orthogonal Non-Canonical Amino acid Tagging (BONCAT) for effective low-disruption labelling of Arabidopsis proteins in vivo.

So many exciting possibilities!

www.biorxiv.org/content/10.1...
May 23, 2025 at 1:38 PM
jcamthrash.bsky.social
BONCAT-Live for isolation and cultivation of active environmental bacteria www.biorxiv.org/content/10.1... #jcampubs
BONCAT-Live for isolation and cultivation of active environmental bacteria
In diverse environments, microbes drive a myriad of processes, from geochemical and nutrient cycling to interspecies interactions, including in association with plants and animals. Their physiological...
www.biorxiv.org
May 15, 2025 at 1:05 PM
jcamthrash.bsky.social
Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesised proteins academic.oup.com/ismecommun/a... #jcampubs
December 4, 2024 at 2:34 PM
jcamthrash.bsky.social
Tracking active heterotrophic microbial communities in the Guaymas Basin deep biosphere with BONCAT-FACS www.biorxiv.org/content/10.6... #jcampubs
December 18, 2025 at 3:09 PM
atanas-g-atanasov.bsky.social
🧵 2/6:
BONCAT-FACS now uncovers translationally active cells in complex environments!

🌐 #BONCAT #FlowCytometry #MicrobialEcology @alisyngayle.bsky.social @alfredodistefano.bsky.social @science-george.bsky.social @spatzlhirn.bsky.social @michaeldonoian.bsky.social @manjeet786.bsky.social
October 17, 2025 at 11:14 AM
achemsynbio.bsky.social
Excited to share our work with Eranthie Weerapana and Tim van Opijnen, co-led by Conor Loynd, Soumya Roy, and Sarah Canarelli! We introduce a new generation of BONCAT tools for the versatile characterization of newly synthesized proteins in pathogenic bacteria. rdcu.be/eI4M5
Cell-selective multiplexed bioorthogonal noncanonical amino acid tagging for nascent proteomics
Nature Chemical Biology - Engineered aminoacyl-tRNA synthetase (aaRS) mutants have been developed that facilitate ultrafast bioorthogonal noncanonical amino acid tagging (BONCAT) of newly...
rdcu.be
October 2, 2025 at 2:10 PM
biorxiv-microbiol.bsky.social
BONCAT-Live for isolation and cultivation of active environmental bacteria https://www.biorxiv.org/content/10.1101/2025.05.14.654084v1
May 15, 2025 at 3:18 AM
atanas-g-atanasov.bsky.social
🧵 1/6:
Explore how BONCAT, SIP & single-cell tools revolutionize microbiome research!
🔗 inpst.net/new-chemical...

🌐 #Microbiome #CRBIOTECH @georgeradics.bsky.social @martinbue.bsky.social @holyunknown.bsky.social @raoulxkessel-aland.bsky.social @hipecsurvivor.bsky.social @shawneexo.bsky.social
October 17, 2025 at 11:13 AM
photosteve.bsky.social
Wow I had a crazy weekend for non-bird sightings. I’m not going to save the best for last or any of that, guys I saw a BOBCAT yesterday morning 😱🤯 #mammals
Bobcat sitting in thick grass, its face is visible behind the grass Boncat emerging from the grass. Its tongue is out 😛 Bobcat licking its lips while sitting in tall grass (it was grooming itself)
October 14, 2024 at 3:58 PM
environmicrobio.bsky.social
Great work in ISMEComm: BONCAT as alternative to microautoradiography (“BONCAT provides estimates of single-cell heterotrophic activity consistent with microautoradiography in deep-ocean samples, supporting its application as a non-radioactive alternative in low-activity environments.”) #microsky🧪🦠
jcamthrash.bsky.social Cameron Thrash @jcamthrash.bsky.social · Mar 2
Single-cell heterotrophic activity in deep-ocean prokaryotic communities quantified by BONCAT and microautoradiography academic.oup.com/ismecommun/a... #jcampubs 🌊
March 2, 2026 at 5:54 PM
ryanziels.bsky.social
I’m very happy this is finally out! Here, we showcase the combination of BONCAT and SIP metaproteomics to uncover rare and active microbes driving anaerobic acetate turnover. We are excited to see what other microbial metabolisms and ecosystems this approach can help to illuminate! 🦠
October 21, 2025 at 5:08 PM
environmicrobio.bsky.social
Viral BONCAT-FACS and single virus genomics. academic.oup.com/ismecommun/a...
March 9, 2026 at 3:50 PM
evolvedbiofilm.bsky.social
Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesised proteins
#ISMEComms by Patrick Hellwig et al
academic.oup.com/ismecommun/a...
Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesised proteins
Abstract. A comprehensive understanding of microbial community dynamics is fundamental to the advancement of environmental microbiology, human health, and
academic.oup.com
December 6, 2024 at 8:34 AM
environmicrobio.bsky.social
Important advancement of BONCAT to achieve the sorting of viable cells and cultivation on solid media plates. This is achieved by incubating environmental samples in the presence of HPG, and sorting active cells via FACS onto plates. Isolates were identified using 16S rRNA gene sequencing.
May 15, 2025 at 2:38 PM
jcamthrash.bsky.social
Tracking active heterotrophic microbial communities in the Guaymas Basin deep biosphere with BONCAT-FACS academic.oup.com/ismecommun/a... #jcampubs 🌊
April 22, 2026 at 3:58 PM
environmicrobio.bsky.social
New lab preprint “Tracking active heterotrophic microbial communities in the Guaymas Basin deep biosphere with BONCAT-FACS” co-first authored by Andrew Montgomery and Sylvia Nupp, co-authors Virginia Edgcomb, Chase Gray, & @dr-zj.bsky.social. Funded by the NSF. 🦠 tinyurl.com/kfxjwj58
Hydrothermal vent sediment in Guaymas Basin
January 1, 2026 at 6:08 PM
plantphys.bsky.social
Optimizing bio-orthogonal non-canonical amino acid tagging (BONCAT) for low-disruption labeling of Arabidopsis proteins in vivo (Nicholas Hassan , Shelly Braun , Mohana Talasila , Curtis Kennedy , Luke Yaremko , Richard P Fahlman , R Glen Uhrig) doi.org/10.1093/plph... #PlantScience @aspbofficial
Optimizing bio-orthogonal non-canonical amino acid tagging (BONCAT) for low-disruption labeling of Arabidopsis proteins in vivo
An optimized means for utilizing BONCAT protein labeling in plants to uncover protein production and degradation responses.
doi.org
August 6, 2026 at 1:21 PM
pastelbio.bsky.social
Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to detect newly synthesized proteins in cells and their secretome journals.plos.org/pl...

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#proteomics #prot-paper
August 16, 2025 at 5:40 PM
jcamthrash.bsky.social
Dual-BONCAT reveals distinct subpopulations of anabolically active cells journals.asm.org/doi/10.1128/... #jcampubs
April 22, 2026 at 4:11 PM
biorxiv-microbiol.bsky.social
Illuminating the active virosphere with BONCAT and single virus genomic sequencing technologies https://www.biorxiv.org/content/10.1101/2025.08.05.666878v1
August 6, 2025 at 3:17 AM
atanas-g-atanasov.bsky.social
🧵 6/6:
This paper is a call to action: Integrate BONCAT, SIP & RACS to map activity cell by cell

🌐 #MicrobiomeScience #OmicsRevolution #FutureOfBiotech @oleksandrkashuba.bsky.social @alokjha09.bsky.social @kennarubin.bsky.social @sole25.bsky.social @haoyin.bsky.social @arnieoflovers.bsky.social
October 17, 2025 at 11:16 AM