Faini et al. develop CLOK, a method that labels neurons across a spectrum of colors according to when they were born, allowing successive generations of brain cells to be tracked as they take shape in...

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What & why Fresh local development accounts alice.test and bob.test currently have neither a birthdate nor age assurance state, so logging into social-app can show the age assurance gate. Seed ...

On exposure to a cell-permeable, fast-binding JF dye ligand, neurons genetically expressing HaloTag become permanently fluorescently labeled, enabling precise visualization of targeted cells27,29 or, as recently shown, identification of newly inserted receptors or synaptic proteins30,31. We first performed a series of control experiments to determine key features and parameters of the CLOK labeling. In a first set of experiments, we examined if all genetically targeted neurons could be labeled with a JF dye in larval zebrafish using a transgenic (Tg) line co-expressing the HaloTag protein and a membrane-localized GFP. After incubation with a JF dye (here, JF525) at 5 days post fertilization (d.p.f.) (Methods), we evaluated the degree of colocalization of the dye and GFP fluorescence, finding 97.43 ± 1.8% of co-expressing neurons (Supplementary Fig. 1a; mean ± s.d., N = 3 fish, 16 planes), confirming robust, near-complete JF dye labeling. Delaying imaging after dye incubation in the same Tg line (incubation at 3 d.p.f. followed by imaging from 3 to 6 d.p.f.) increased the number of JF dye-negative, GFP-positive cells located at the periphery of the tectum (Supplementary Fig. 1b). This observation confirms that the HaloTag-JF dye complex remains stable, preventing JF dye from binding to newly expressed HaloTag...

Thank y’all, including for the info re: Proton. Thus far, there’s been no request for ID, but how much longer, I don’t know. Since user1 mentioned the 15gb gmail limit, I probably do have room to transfer all my years of gmails (of my 3 gmail accounts), but meanwhile, I had entered my birthdate in my junkmail account a few days ago, and thought I was out of the woods. But despite having entered my birthdate, just now, gmail asked me to enter the verification code sent to my recovery email (which is my “acquaintance” gmailbox). Then when i tried logging on to my “acquaintance” gmailbox, it asked me to enter my recovery email (which is my junkmail gmail address). They also asked me to enter my birthdate for my “acquaintance” gmailbox, after which i had to click CONFIRM that i’m age blah-blah… I finally was able to gain entry to both those gmail boxes. It just doesn’t stop. I assure you there is zero privacy in my 3 gmail accounts. It’s too late by now. The only thing which would ever recover privacy would frankly be a solar flare or something of the sort. P.S. I used to use both the vintage YahooMail and vintage Gmail. Then when i heard Verizon was taking over Yahoo, I panicked and asked around. At that time, lots of onliners advised me to transfer to Gmail, so i transferred my primary-Yahoomail and my junkmail-Yahoomail to separate Gmail accounts. I forget if i already had my acquaintance-Gmail account or created it at time of transfer. It was a major ordeal, but finally got done. Now i’m having DejaVu all over again, because Gmail has turned rogue for quite awhile already. Anyway, I guess that about wraps this up. Thanks again!!

> I know Graphene has the duress PIN, which some have suggested setting to something obvious like “1234” or your birthdate so that if an adversary tries to unlock your phone they wipe it instead, Would any adversary believe a GrapheneOS user would have an easy-to-guess PIN?

Remembering Jerry Lee Lewis in advance of his birthdate tomorrow. —Ed. For Jerry Lee Lewis, 1973 was the worst of years and the best of years too; despite a brief turn in prison, the death of a son, a...