Proteasomal control of anti-CRISPRs for the regulation of CRISPR-Cas9 activity using Cas9-ACROBAT
Proteasomal control of anti-CRISPRs for the regulation of CRISPR-Cas9 activity using Cas9-ACROBAT
Martin et al. develop Cas9-ACROBAT, a dual-layered platform combining small-molecule control of anti-CRISPR proteins coupled with riboswitch-regulated guide RNAs. This multi-input strategy enables rapid, reversible, and tightly tuned regulation of CRISPR activity in human cells, while minimizing background leakiness.
dlvr.it
November 10, 2026 at 7:49 PM
Proteasomal control of anti-CRISPRs for the regulation of CRISPR-Cas9 activity using Cas9-ACROBAT
RRIDs were included in this in Stem Cell Research paper. We value the author's support of reproducibility. #reproducibility #BetterScience #RRID
Generation of CD43-P2A-mNeonGreen reporter human pluripotent stem cell line using CRISPR Cas9
Read the full paper: Generation of CD43-P2A-mNeonGreen reporter human pluripotent stem cell line using CRISPR Cas9
doi.org
November 8, 2026 at 8:01 AM
RRIDs were included in this in Stem Cell Research paper. We value the author's support of reproducibility. #reproducibility #BetterScience #RRID
AI integration into CRISPR-Cas9 genome editing is advancing precision and safety, key for medical applications from genetic disorders to regenerative medicine.
🔗 Source: https://pubmed.ncbi.nlm.nih.gov/42987985/
🔗 Source: https://pubmed.ncbi.nlm.nih.gov/42987985/
November 7, 2026 at 9:36 AM
AI integration into CRISPR-Cas9 genome editing is advancing precision and safety, key for medical applications from genetic disorders to regenerative medicine.
🔗 Source: https://pubmed.ncbi.nlm.nih.gov/42987985/
🔗 Source: https://pubmed.ncbi.nlm.nih.gov/42987985/
Check out our paper on therapeutic vulnerabilities of the human fungal pathogen Cryptococcus neoformans. We describe a method we call CRISPR-insert for genome mutagenesis. This is the first genome-wide CRISPR/Cas9 method implemented and validated in any fungal pathogen (to our knowledge). 1/3
November 5, 2026 at 9:55 PM
Check out our paper on therapeutic vulnerabilities of the human fungal pathogen Cryptococcus neoformans. We describe a method we call CRISPR-insert for genome mutagenesis. This is the first genome-wide CRISPR/Cas9 method implemented and validated in any fungal pathogen (to our knowledge). 1/3
Congrats to Prof. Giulia Palermo on receiving an NIH/NIGMS ESI MIRA (R35) award! The grant will support the Palermo Labs' (@giuliapalermo.bsky.social) work integrating AI and molecular simulations to advance more efficient and precise CRISPR-Cas9 genome editing. Link: ucla.in/4ezv6Ld
November 9, 2026 at 6:12 PM
Congrats to Prof. Giulia Palermo on receiving an NIH/NIGMS ESI MIRA (R35) award! The grant will support the Palermo Labs' (@giuliapalermo.bsky.social) work integrating AI and molecular simulations to advance more efficient and precise CRISPR-Cas9 genome editing. Link: ucla.in/4ezv6Ld
RRIDs were included in this in bioRxiv paper. We value the author's support of reproducibility. #BetterScience #OpenResearch #reproducibility
High-resolution CRISPR/Cas9 screens identify PAK2 as a suppressor of macrophage proliferation
Read the full paper: High-resolution CRISPR/Cas9 screens identify PAK2 as a suppressor of macrophage proliferation
doi.org
November 7, 2026 at 8:01 AM
RRIDs were included in this in bioRxiv paper. We value the author's support of reproducibility. #BetterScience #OpenResearch #reproducibility
Cas9 DSBs are often repaired by sister chromatid exchange—invisible to conventional WGS. sci-L3-Strand-seq maps Cas9-induced SCE genome-wide across single and >200 sites in human cells, linking hidden SCE to structural variation risk.
https://doi.org/10.7554/eLife.112073
https://doi.org/10.7554/eLife.112073
November 9, 2026 at 1:23 AM
Cas9 DSBs are often repaired by sister chromatid exchange—invisible to conventional WGS. sci-L3-Strand-seq maps Cas9-induced SCE genome-wide across single and >200 sites in human cells, linking hidden SCE to structural variation risk.
https://doi.org/10.7554/eLife.112073
https://doi.org/10.7554/eLife.112073
NanoPAMDA enables rapid PAM characterization of metagenome-derived Cas9 effectors https://www.biorxiv.org/content/10.64898/2026.11.04.764188v1
November 5, 2026 at 11:32 AM
NanoPAMDA enables rapid PAM characterization of metagenome-derived Cas9 effectors https://www.biorxiv.org/content/10.64898/2026.11.04.764188v1
CRV Science News
🧬 CRISPR-Cas9 Depletion of Ubiquitous Ribosomal and Mitochondrial cDNA Halves Sequencing Depth Requirements in Human Intestinal Single-Cell Transcriptomics — Single-cell RNA sequencing (scRNA-seq) has transformed m…
https://crvscience.com/news/crispr-cas9-rna-depletion-intestinal
🧬 CRISPR-Cas9 Depletion of Ubiquitous Ribosomal and Mitochondrial cDNA Halves Sequencing Depth Requirements in Human Intestinal Single-Cell Transcriptomics — Single-cell RNA sequencing (scRNA-seq) has transformed m…
https://crvscience.com/news/crispr-cas9-rna-depletion-intestinal
CRV Science News
🧬 CRISPR-Cas9 Depletion of Ubiquitous Ribosomal and Mitochondrial cDNA Halves Sequencing Depth Requirements in Human Intestinal Single-Cell Transcriptomics — Single-cell RNA sequencing (scRNA-seq) has transformed mucosal immunology and gastroenterology by resolving the transcript…
crvscience.com
November 10, 2026 at 11:15 PM
CRV Science News
🧬 CRISPR-Cas9 Depletion of Ubiquitous Ribosomal and Mitochondrial cDNA Halves Sequencing Depth Requirements in Human Intestinal Single-Cell Transcriptomics — Single-cell RNA sequencing (scRNA-seq) has transformed m…
https://crvscience.com/news/crispr-cas9-rna-depletion-intestinal
🧬 CRISPR-Cas9 Depletion of Ubiquitous Ribosomal and Mitochondrial cDNA Halves Sequencing Depth Requirements in Human Intestinal Single-Cell Transcriptomics — Single-cell RNA sequencing (scRNA-seq) has transformed m…
https://crvscience.com/news/crispr-cas9-rna-depletion-intestinal
🌱 How can we breed more resilient soybeans?
This review shows how #CRISPR #GenomeEditing can help improve stress tolerance, nutrition and key crop traits, and addresses technical and regulatory challenges.
doi.org/10.3389/fgee...
This review shows how #CRISPR #GenomeEditing can help improve stress tolerance, nutrition and key crop traits, and addresses technical and regulatory challenges.
doi.org/10.3389/fgee...
Frontiers | CRISPR/Cas9 in soybean research: a measured leap toward genetic refinement
Soybean [Glycine max (Linn.) Merr] is a globally important source of plant-based protein and edible oil, widely cultivated in India as a major oilseed crop. ...
doi.org
November 9, 2026 at 2:25 PM
🌱 How can we breed more resilient soybeans?
This review shows how #CRISPR #GenomeEditing can help improve stress tolerance, nutrition and key crop traits, and addresses technical and regulatory challenges.
doi.org/10.3389/fgee...
This review shows how #CRISPR #GenomeEditing can help improve stress tolerance, nutrition and key crop traits, and addresses technical and regulatory challenges.
doi.org/10.3389/fgee...
The study replaced Cas9 with Cas12a to deliver a DNA-destroying enzyme to tumor cells once specific mutations are detected. This mechanism converts a detection event into a guided missile-like destruction of the cell's genetic material.
#Crispr #Cas12a #Cancer
#Crispr #Cas12a #Cancer
Researchers replaced Cas9 with Cas12a to destroy DNA
Source: Hackaday
realfeedapp.com
November 11, 2026 at 7:20 AM
In 2012, scientists turned a bacterial defense against viruses into DNA scissors: a guide RNA leads Cas9 to a chosen spot, and it cuts.
2020: Nobel Prize for Charpentier and Doudna. 2023: the first CRISPR therapy, for sickle cell disease.
#CRISPR #Science #GeneEditing
2020: Nobel Prize for Charpentier and Doudna. 2023: the first CRISPR therapy, for sickle cell disease.
#CRISPR #Science #GeneEditing
November 9, 2026 at 2:14 PM
In 2012, scientists turned a bacterial defense against viruses into DNA scissors: a guide RNA leads Cas9 to a chosen spot, and it cuts.
2020: Nobel Prize for Charpentier and Doudna. 2023: the first CRISPR therapy, for sickle cell disease.
#CRISPR #Science #GeneEditing
2020: Nobel Prize for Charpentier and Doudna. 2023: the first CRISPR therapy, for sickle cell disease.
#CRISPR #Science #GeneEditing
And of course there were intervening steps, not the least of which was the development of that discovery into the Nobel-winning CRISPR-Cas9 tool by Jennifer Doudna and Emmanuelle Charpentier in 2012, and the teams at Vertex Pharmaceuticals and CRISPR Therapeutics who turned it into "Casgevy"
November 10, 2026 at 1:43 PM
And of course there were intervening steps, not the least of which was the development of that discovery into the Nobel-winning CRISPR-Cas9 tool by Jennifer Doudna and Emmanuelle Charpentier in 2012, and the teams at Vertex Pharmaceuticals and CRISPR Therapeutics who turned it into "Casgevy"
The authors included RRIDs in their in Applied Microbiology and Biotechnology paper! RRIDs improve reproducibility in scientific research. #ReproducibleResearch #BetterScience #RRID
An improved CRISPR-Cas9 protein-based method for knocking out insect Sf9 cell genes
Read the full paper: An improved CRISPR-Cas9 protein-based method for knocking out insect Sf9 cell genes
doi.org
November 6, 2026 at 8:00 AM
The authors included RRIDs in their in Applied Microbiology and Biotechnology paper! RRIDs improve reproducibility in scientific research. #ReproducibleResearch #BetterScience #RRID
NanoPAMDA enables rapid PAM characterization of metagenome-derived Cas9 effectors https://www.biorxiv.org/content/10.64898/2026.11.04.764188v1
November 5, 2026 at 11:32 AM
NanoPAMDA enables rapid PAM characterization of metagenome-derived Cas9 effectors https://www.biorxiv.org/content/10.64898/2026.11.04.764188v1
📈 Beyond Conventional CRISPR: Next-Generation Gene Editing
While conventional CRISPR-Cas9 treatments have reached the market, pioneers are advancing alternative methodologies.
🔗 Read the full story on GazeWire
#Markets #Biotech #Crispr #GazeWire
While conventional CRISPR-Cas9 treatments have reached the market, pioneers are advancing alternative methodologies.
🔗 Read the full story on GazeWire
#Markets #Biotech #Crispr #GazeWire
📈 Beyond Conventional CRISPR: Next-Generation Gene Editing
While conventional CRISPR-Cas9 treatments have reached the market, pioneers are advancing alternative methodologies.
gazewire.com
November 6, 2026 at 2:35 AM
📈 Beyond Conventional CRISPR: Next-Generation Gene Editing
While conventional CRISPR-Cas9 treatments have reached the market, pioneers are advancing alternative methodologies.
🔗 Read the full story on GazeWire
#Markets #Biotech #Crispr #GazeWire
While conventional CRISPR-Cas9 treatments have reached the market, pioneers are advancing alternative methodologies.
🔗 Read the full story on GazeWire
#Markets #Biotech #Crispr #GazeWire
Job ID: 26FA0535
Extract: Postdoctoral position in Rutgers-Camden Biology to study EGFR signaling evolution using CRISPR/Cas9 and molecular biology in Drosophila. NIH-funded, renewable based on funding.
Extract: Postdoctoral position in Rutgers-Camden Biology to study EGFR signaling evolution using CRISPR/Cas9 and molecular biology in Drosophila. NIH-funded, renewable based on funding.
November 9, 2026 at 3:21 AM
Job ID: 26FA0535
Extract: Postdoctoral position in Rutgers-Camden Biology to study EGFR signaling evolution using CRISPR/Cas9 and molecular biology in Drosophila. NIH-funded, renewable based on funding.
Extract: Postdoctoral position in Rutgers-Camden Biology to study EGFR signaling evolution using CRISPR/Cas9 and molecular biology in Drosophila. NIH-funded, renewable based on funding.
🧬 New preprint! NanoPAMDA profiles PAM preferences of many Cas9 orthologs at once on a Nanopore.
We mined 620k genomes + human microbiome reads and profiled 14 candidates. E10, from a mouse gut metagenome, is functional, with an NGG-like PAM. www.biorxiv.org/content/10.6...
We mined 620k genomes + human microbiome reads and profiled 14 candidates. E10, from a mouse gut metagenome, is functional, with an NGG-like PAM. www.biorxiv.org/content/10.6...
November 6, 2026 at 7:37 AM
🧬 New preprint! NanoPAMDA profiles PAM preferences of many Cas9 orthologs at once on a Nanopore.
We mined 620k genomes + human microbiome reads and profiled 14 candidates. E10, from a mouse gut metagenome, is functional, with an NGG-like PAM. www.biorxiv.org/content/10.6...
We mined 620k genomes + human microbiome reads and profiled 14 candidates. E10, from a mouse gut metagenome, is functional, with an NGG-like PAM. www.biorxiv.org/content/10.6...
The sperm-specific phospholipase C isoform PLCζ1 initiates fertilization Ca2+ oscillations in mammals, yet its loss does not eliminate #fertility. This study shows that PLCδ4 is a crucial backup oscillator supporting robust fertilization in mammals.
🧪 #AcademicSky #reproduction
plos.io/3VaGHtq
🧪 #AcademicSky #reproduction
plos.io/3VaGHtq
November 9, 2026 at 5:04 PM
The sperm-specific phospholipase C isoform PLCζ1 initiates fertilization Ca2+ oscillations in mammals, yet its loss does not eliminate #fertility. This study shows that PLCδ4 is a crucial backup oscillator supporting robust fertilization in mammals.
🧪 #AcademicSky #reproduction
plos.io/3VaGHtq
🧪 #AcademicSky #reproduction
plos.io/3VaGHtq
IOCAS researchers established the first vector-based #CRISPR-Cas9 platform in green tide macroalga Ulva prolifera, driven by dual-transcribing U6 promoters that trigger 92.3% editing efficiency and targeted MULE transposon insertions. 🌿🤖 #UlvaProlifera #Macroalgae #BioRxiv2026 #MarineBiotech #CAS
November 10, 2026 at 1:50 AM
IOCAS researchers established the first vector-based #CRISPR-Cas9 platform in green tide macroalga Ulva prolifera, driven by dual-transcribing U6 promoters that trigger 92.3% editing efficiency and targeted MULE transposon insertions. 🌿🤖 #UlvaProlifera #Macroalgae #BioRxiv2026 #MarineBiotech #CAS
The sperm-specific phospholipase C isoform PLCζ1 initiates fertilization Ca2+ oscillations in mammals, yet its loss does not eliminate #fertility. This study shows that PLCδ4 is a crucial backup oscillator supporting robust fertilization in mammals.
🧪 #AcademicSky #reproduction
plos.io/3VaGHtq
🧪 #AcademicSky #reproduction
plos.io/3VaGHtq
November 8, 2026 at 1:02 PM
The sperm-specific phospholipase C isoform PLCζ1 initiates fertilization Ca2+ oscillations in mammals, yet its loss does not eliminate #fertility. This study shows that PLCδ4 is a crucial backup oscillator supporting robust fertilization in mammals.
🧪 #AcademicSky #reproduction
plos.io/3VaGHtq
🧪 #AcademicSky #reproduction
plos.io/3VaGHtq
Genetic disruption of cutaneous de novo glucocorticoid synthesis: a CRISPR-Cas9 CYP11B1-knockout human epidermal model https://www.biorxiv.org/content/10.64898/2026.11.03.764005v1
November 4, 2026 at 1:30 PM
Genetic disruption of cutaneous de novo glucocorticoid synthesis: a CRISPR-Cas9 CYP11B1-knockout human epidermal model https://www.biorxiv.org/content/10.64898/2026.11.03.764005v1