Such a small change does make a dramatic visual difference.
Aligns with one of my creative ideals: Compelling Evocation Through Simply Application #CETSA
Aligns with one of my creative ideals: Compelling Evocation Through Simply Application #CETSA
I've always said that Voyager needed longer nacelles over the short stubby one they had.
Perhaps the nacelle size is why they predicted 75 years travel time...
Perhaps the nacelle size is why they predicted 75 years travel time...
September 23, 2026 at 4:08 AM
Such a small change does make a dramatic visual difference.
Aligns with one of my creative ideals: Compelling Evocation Through Simply Application #CETSA
Aligns with one of my creative ideals: Compelling Evocation Through Simply Application #CETSA
March 29, 2024 at 4:09 AM
Molecular Targeted Engagement of DPP9 in Rat Tissue Using CETSA, SP3 Processing, and Absolute Quantitation Mass Spectrometry #ACSChemBiol pubs.acs.org/doi/10.1021/...
Molecular Targeted Engagement of DPP9 in Rat Tissue Using CETSA, SP3 Processing, and Absolute Quantitation Mass Spectrometry
The cellular thermal shift assay (CETSA) provides a means of understanding the extent to which a small molecule ligand associates with a protein target of therapeutic interest, thereby inferring targe...
pubs.acs.org
December 7, 2024 at 6:59 PM
Molecular Targeted Engagement of DPP9 in Rat Tissue Using CETSA, SP3 Processing, and Absolute Quantitation Mass Spectrometry #ACSChemBiol pubs.acs.org/doi/10.1021/...
Ji'Goro: "Don't worry boss person, we'll do the things in the places!"
Lt. Tardigrade: "Flush keys, bite terminals. We be most successful!"
Iconian: "..."
Lt. Tardigrade: "Flush keys, bite terminals. We be most successful!"
Iconian: "..."
June 4, 2025 at 12:31 PM
Ji'Goro: "Don't worry boss person, we'll do the things in the places!"
Lt. Tardigrade: "Flush keys, bite terminals. We be most successful!"
Iconian: "..."
Lt. Tardigrade: "Flush keys, bite terminals. We be most successful!"
Iconian: "..."
Having first Starbound adventure with @cdr-oconnor.bsky.social . Result:
February 10, 2025 at 5:47 AM
Having first Starbound adventure with @cdr-oconnor.bsky.social . Result:
*Lt. Tardigrade leaps in with a sword to chase nightmares away. This does little for the headache. Cetsa is on hand with comfy blanket for that.*
September 19, 2025 at 12:30 PM
*Lt. Tardigrade leaps in with a sword to chase nightmares away. This does little for the headache. Cetsa is on hand with comfy blanket for that.*
High-Throughput CETSA Identifies Small Molecule Modulators of ILT3 (LILRB4) with Functional Activity in Human iPSC-Derived Microglia for Alzheimers Disease https://www.biorxiv.org/content/10.64898/2026.05.19.726383v1
May 22, 2026 at 6:01 AM
High-Throughput CETSA Identifies Small Molecule Modulators of ILT3 (LILRB4) with Functional Activity in Human iPSC-Derived Microglia for Alzheimers Disease https://www.biorxiv.org/content/10.64898/2026.05.19.726383v1
Our malaria complexome paper is finally out! We mapped the P. falciparum malaria complexome in asexual blood stages, validated a couple of new complexes and investigated the dynamics of protein complexes across the IDC! A short thread (1/14). bit.ly/4om8kIu #malaria #proteomics #teammassspec #CETSA
MAP-X reveals distinct protein complex dynamics across Plasmodium falciparum blood stages - Nature Microbiology
MAP-X is used to map protein complexes in P. falciparum, identifying previously undescribed interactions, revealing stage-specific dynamics and predicting moonlighting behaviour of subunits.
bit.ly
November 28, 2025 at 5:10 PM
Our malaria complexome paper is finally out! We mapped the P. falciparum malaria complexome in asexual blood stages, validated a couple of new complexes and investigated the dynamics of protein complexes across the IDC! A short thread (1/14). bit.ly/4om8kIu #malaria #proteomics #teammassspec #CETSA
RRIDs were included in this Nature Communications paper. Thanks for making your methods matter! #RRID #reproducibility #accelerateopenscience
MS CETSA deep functional proteomics uncovers DNA repair programs leading to gemcitabine resistance
doi.org
May 23, 2025 at 7:00 AM
RRIDs were included in this Nature Communications paper. Thanks for making your methods matter! #RRID #reproducibility #accelerateopenscience
The authors included RRIDs in their iScience paper! Thanks for making your methods matter! #RRID #reproducibility #OpenScience
CETSA-MS unveils novel targets engaged by rigosertib to promote anti-tumor activity and inflammatory responses
doi.org
June 1, 2025 at 7:00 AM
The authors included RRIDs in their iScience paper! Thanks for making your methods matter! #RRID #reproducibility #OpenScience
ER stress tolerance is regulated by copper-dependent PERK kinase activity
ER stress tolerance is regulated by copper-dependent PERK kinase activity
Newton et al. establish PERK as a copper-regulated kinase. The copper dependency of PERK kinase activity is characterized in vitro utilizing glutathione pull-down assays, kinase assays, and CETSA. The physiological implications of this regulatory paradigm for the modulation of ER stress tolerance are demonstrated in cellular and animal models.
dlvr.it
October 7, 2025 at 12:36 PM
ER stress tolerance is regulated by copper-dependent PERK kinase activity
investigated by integrating molecular docking, molecular dynamics simulation, drug affinity responsive target stability (DARTS) assays, and cellular thermal shift assays (CETSA). A lipopolysaccharide (LPS)-induced BV2 cell model was established, with additional interventions (🧵 6/16)
July 11, 2026 at 9:51 AM
investigated by integrating molecular docking, molecular dynamics simulation, drug affinity responsive target stability (DARTS) assays, and cellular thermal shift assays (CETSA). A lipopolysaccharide (LPS)-induced BV2 cell model was established, with additional interventions (🧵 6/16)
Molecular dynamics simulations further showed that their binding free energies were -23.31 and -22.06 kcal/mol, respectively, which were more favorable than those of the corresponding cross-combinations. In DARTS and CETSA assays, Ben enhanced the protease resistance and thermal (🧵 13/16)
July 11, 2026 at 9:51 AM
Molecular dynamics simulations further showed that their binding free energies were -23.31 and -22.06 kcal/mol, respectively, which were more favorable than those of the corresponding cross-combinations. In DARTS and CETSA assays, Ben enhanced the protease resistance and thermal (🧵 13/16)
분자 도킹, 분자 동역학 시뮬레이션, 약물 친화도 반응 표적 안정성(DARTS) 분석 및 세포 열 이동 분석(CETSA)을 통합하여 후보 성분과 그 추정 표적 간의 연관성을 추가로 조사했다. 리포폴리사카라이드(LPS) 유도 BV2 세포 모델이 구축되었으며, P2Y12R 작용제 2-MeS-ADP 및 PPARγ 억제제 GW9662를 이용한 추가적인 개입이 이루어졌습니다. (🧵 4/10)
July 11, 2026 at 9:43 AM
분자 도킹, 분자 동역학 시뮬레이션, 약물 친화도 반응 표적 안정성(DARTS) 분석 및 세포 열 이동 분석(CETSA)을 통합하여 후보 성분과 그 추정 표적 간의 연관성을 추가로 조사했다. 리포폴리사카라이드(LPS) 유도 BV2 세포 모델이 구축되었으며, P2Y12R 작용제 2-MeS-ADP 및 PPARγ 억제제 GW9662를 이용한 추가적인 개입이 이루어졌습니다. (🧵 4/10)
DARTS 및 CETSA 분석에서, Ben은 P2Y12R의 프로테아제 저항성과 열 안정성을 향상시킨 반면, SENI는 PPARγ의 프로테아제 저항성과 열 안정성을 향상시켰습니다. 역전 실험에서, 2-MeS-ADP는 Ben 그룹에 비해 식세포 활성을 40.6%, P2Y12R 발현을 76.8% 각각 증가시켰으며; GW9662는 SENI 군에 비해 식세포 활성을 41.7% 증가시키고 PPARγ 발현을 26.8% 감소시켰다. (🧵 9/10)
July 11, 2026 at 9:43 AM
DARTS 및 CETSA 분석에서, Ben은 P2Y12R의 프로테아제 저항성과 열 안정성을 향상시킨 반면, SENI는 PPARγ의 프로테아제 저항성과 열 안정성을 향상시켰습니다. 역전 실험에서, 2-MeS-ADP는 Ben 그룹에 비해 식세포 활성을 40.6%, P2Y12R 발현을 76.8% 각각 증가시켰으며; GW9662는 SENI 군에 비해 식세포 활성을 41.7% 증가시키고 PPARγ 발현을 26.8% 감소시켰다. (🧵 9/10)
Target Identification of Ginsenosides Against Cognitive Impairment by Using Mass Spectrometry-Based Cellular Thermal Shift Assay (CETSA) #NeurochemRes link.springer.com/article/10.1...
Target Identification of Ginsenosides Against Cognitive Impairment by Using Mass Spectrometry-Based Cellular Thermal Shift Assay (CETSA) - Neurochemical Research
Ginsenosides, the primary bioactive constituents of ginseng, are recognized for its potential to mitigate cognitive deficits associated with neurodegenerative conditions. Nevertheless, its molecular t...
link.springer.com
May 5, 2025 at 7:00 PM
Target Identification of Ginsenosides Against Cognitive Impairment by Using Mass Spectrometry-Based Cellular Thermal Shift Assay (CETSA) #NeurochemRes link.springer.com/article/10.1...
How selective is your compound once it’s inside a cell?
Read Promega’s blog from Tian Yang & Kristin Huwiler exploring how CETSA-MS, chemical #proteomics & NanoBRET® Target Engagement #assays are reshaping how we study compound selectivity in live cells:
Read Promega’s blog from Tian Yang & Kristin Huwiler exploring how CETSA-MS, chemical #proteomics & NanoBRET® Target Engagement #assays are reshaping how we study compound selectivity in live cells:
Cellular Selectivity Profiling: Unveiling Novel Interactions and More Accurate Compound Specificity - Promega Connections
NanoBRET® Target Engagement assays are a great alternative to mass spec-based cell selectivity approaches.
tinyurl.com
November 20, 2025 at 9:08 AM
How selective is your compound once it’s inside a cell?
Read Promega’s blog from Tian Yang & Kristin Huwiler exploring how CETSA-MS, chemical #proteomics & NanoBRET® Target Engagement #assays are reshaping how we study compound selectivity in live cells:
Read Promega’s blog from Tian Yang & Kristin Huwiler exploring how CETSA-MS, chemical #proteomics & NanoBRET® Target Engagement #assays are reshaping how we study compound selectivity in live cells:
Determination of the cellular target engagement by direct-to-biology Cellular Thermal Shift Assay (CETSA) https://www.biorxiv.org/content/10.64898/2026.02.28.708721v1
March 2, 2026 at 10:30 AM
Determination of the cellular target engagement by direct-to-biology Cellular Thermal Shift Assay (CETSA) https://www.biorxiv.org/content/10.64898/2026.02.28.708721v1
Yeah.. Just DM'd you..
I guess TPP patent covers anything where temp induced denaturation is assessed in a total protein background for ligand binding in a multiplexed workflow.. Single protein CETSA is feasible outside of the patent.. Also TPP patent restricts only commercial usage..
I guess TPP patent covers anything where temp induced denaturation is assessed in a total protein background for ligand binding in a multiplexed workflow.. Single protein CETSA is feasible outside of the patent.. Also TPP patent restricts only commercial usage..
December 14, 2024 at 7:18 PM
Yeah.. Just DM'd you..
I guess TPP patent covers anything where temp induced denaturation is assessed in a total protein background for ligand binding in a multiplexed workflow.. Single protein CETSA is feasible outside of the patent.. Also TPP patent restricts only commercial usage..
I guess TPP patent covers anything where temp induced denaturation is assessed in a total protein background for ligand binding in a multiplexed workflow.. Single protein CETSA is feasible outside of the patent.. Also TPP patent restricts only commercial usage..
Quantitative target engagement of RIPK1 in human whole blood via the cellular thermal shift assay for potential pre-clinical and clinical applications https://www.biorxiv.org/content/10.1101/2023.10.02.560523v1
Quantitative target engagement of RIPK1 in human whole blood via the cellular thermal shift assay for potential pre-clinical and clinical applications https://www.biorxiv.org/content/10.1101/2023.10.02.560523v1
The cellular thermal shift assay (CETSA(R)) is a target engagement method widely used for preclinica
www.biorxiv.org
October 3, 2023 at 3:22 AM
Quantitative target engagement of RIPK1 in human whole blood via the cellular thermal shift assay for potential pre-clinical and clinical applications https://www.biorxiv.org/content/10.1101/2023.10.02.560523v1
High-Throughput CETSA Identifies Small Molecule Modulators of ILT3 (LILRB4) with Functional Activity in Human iPSC-Derived Microglia for Alzheimers Disease https://www.biorxiv.org/content/10.64898/2026.05.19.726383v1
May 22, 2026 at 6:01 AM
High-Throughput CETSA Identifies Small Molecule Modulators of ILT3 (LILRB4) with Functional Activity in Human iPSC-Derived Microglia for Alzheimers Disease https://www.biorxiv.org/content/10.64898/2026.05.19.726383v1
Determination of the cellular target engagement by direct-to-biology Cellular Thermal Shift Assay (CETSA) https://www.biorxiv.org/content/10.64898/2026.02.28.708721v1
March 2, 2026 at 10:30 AM
Determination of the cellular target engagement by direct-to-biology Cellular Thermal Shift Assay (CETSA) https://www.biorxiv.org/content/10.64898/2026.02.28.708721v1
Quantitative target engagement of RIPK1 in human whole blood via the cellular thermal shift assay for potential pre-clinical and clinical applications https://www.biorxiv.org/content/10.1101/2023.10.02.560523v1
Quantitative target engagement of RIPK1 in human whole blood via the cellular thermal shift assay for potential pre-clinical and clinical applications https://www.biorxiv.org/content/10.1101/2023.10.02.560523v1
The cellular thermal shift assay (CETSA(R)) is a target engagement method widely used for preclinica
www.biorxiv.org
October 3, 2023 at 3:22 AM
Quantitative target engagement of RIPK1 in human whole blood via the cellular thermal shift assay for potential pre-clinical and clinical applications https://www.biorxiv.org/content/10.1101/2023.10.02.560523v1
6/14 (Yes, we made the MAPX pipeline available as an R package) github.com/SamPazicky/M...
GitHub - SamPazicky/MAPX: Use CETSA data for PPI prediction.
Use CETSA data for PPI prediction. Contribute to SamPazicky/MAPX development by creating an account on GitHub.
github.com
November 28, 2025 at 5:10 PM
6/14 (Yes, we made the MAPX pipeline available as an R package) github.com/SamPazicky/M...
