A pyramidal neuron from the prefrontal cortex. #microscopymonday
Labelled with DiI, imaged with the FV3000 CLSM, & depicted using @kwolbachia.bsky.social new KTZ_bw_kawa LUT.
Labelled with DiI, imaged with the FV3000 CLSM, & depicted using @kwolbachia.bsky.social new KTZ_bw_kawa LUT.
January 27, 2025 at 5:38 AM
A pyramidal neuron from the prefrontal cortex. #microscopymonday
Labelled with DiI, imaged with the FV3000 CLSM, & depicted using @kwolbachia.bsky.social new KTZ_bw_kawa LUT.
Labelled with DiI, imaged with the FV3000 CLSM, & depicted using @kwolbachia.bsky.social new KTZ_bw_kawa LUT.
Vanessa is the perfect weapon to protect her sister.
Until she wasn't. Wrecked with guilt, Vanessa and her crew set off to get revenge against the vampire that killed her sister.
But monsters have many forms.
archiveofourown.org/works/89664836
Rather listen? www.youtube.com/watch?v=ClsM...
Until she wasn't. Wrecked with guilt, Vanessa and her crew set off to get revenge against the vampire that killed her sister.
But monsters have many forms.
archiveofourown.org/works/89664836
Rather listen? www.youtube.com/watch?v=ClsM...
August 1, 2026 at 2:14 PM
Vanessa is the perfect weapon to protect her sister.
Until she wasn't. Wrecked with guilt, Vanessa and her crew set off to get revenge against the vampire that killed her sister.
But monsters have many forms.
archiveofourown.org/works/89664836
Rather listen? www.youtube.com/watch?v=ClsM...
Until she wasn't. Wrecked with guilt, Vanessa and her crew set off to get revenge against the vampire that killed her sister.
But monsters have many forms.
archiveofourown.org/works/89664836
Rather listen? www.youtube.com/watch?v=ClsM...
song: CLSM & Cube::Hard - See You On The Other Side
October 3, 2025 at 1:37 AM
song: CLSM & Cube::Hard - See You On The Other Side
John Peel 2003 Festive 50
#JohnPeel
CLSM - John Peel Is Not Enough
Fucking dreadful @willmcalpine.bsky.social 😂
#JohnPeel
CLSM - John Peel Is Not Enough
Fucking dreadful @willmcalpine.bsky.social 😂
CLSM - John Peel Is Not Enough
YouTube video by FumDumBum
youtu.be
December 5, 2025 at 11:36 PM
John Peel 2003 Festive 50
#JohnPeel
CLSM - John Peel Is Not Enough
Fucking dreadful @willmcalpine.bsky.social 😂
#JohnPeel
CLSM - John Peel Is Not Enough
Fucking dreadful @willmcalpine.bsky.social 😂
One week left to apply for a group leader position at the Laboratory of Biophysics (tenure track). Access to excellent imaging facilities (CLSM, STED, FLIM, Multi-photon, SMLM, smFRET, Raman, MRI,…) and more. #jobs
#Jobs We are looking for a new colleague! Tenure Track Assistant Professor in Biophysics in the domains of functional imaging and biophysics. More info here: www.wur.nl/en/vacancy/a...
Assistant Professor in Biophysics
www.wur.nl
February 20, 2025 at 8:15 AM
One week left to apply for a group leader position at the Laboratory of Biophysics (tenure track). Access to excellent imaging facilities (CLSM, STED, FLIM, Multi-photon, SMLM, smFRET, Raman, MRI,…) and more. #jobs
Posting this for no particular reason, but I should be doing vacation stuff and instead I'm looking through some scans I made last week. Here is a very rough brain of a Pieris brassicae butterfly 🦋, about 2 mm wide. CLSM image, visualized with BigVolumeBrowser in Fiji
#Entomology
#Entomology
August 3, 2026 at 8:24 PM
Posting this for no particular reason, but I should be doing vacation stuff and instead I'm looking through some scans I made last week. Here is a very rough brain of a Pieris brassicae butterfly 🦋, about 2 mm wide. CLSM image, visualized with BigVolumeBrowser in Fiji
#Entomology
#Entomology
Mid-week #mycorrhiza 🍄
Just a particularly pretty arbuscule in a rice root cell colonised by the mycorrhizal fungus Rhizophagus irregularis, with a cool view down an intercellular hypha. Stained with WGA-Alexafluor488, imaged by CLSM, false-coloured by depth in root 🔥
#microscopy #mycosci #fungi
Just a particularly pretty arbuscule in a rice root cell colonised by the mycorrhizal fungus Rhizophagus irregularis, with a cool view down an intercellular hypha. Stained with WGA-Alexafluor488, imaged by CLSM, false-coloured by depth in root 🔥
#microscopy #mycosci #fungi
June 12, 2025 at 8:24 AM
Mid-week #mycorrhiza 🍄
Just a particularly pretty arbuscule in a rice root cell colonised by the mycorrhizal fungus Rhizophagus irregularis, with a cool view down an intercellular hypha. Stained with WGA-Alexafluor488, imaged by CLSM, false-coloured by depth in root 🔥
#microscopy #mycosci #fungi
Just a particularly pretty arbuscule in a rice root cell colonised by the mycorrhizal fungus Rhizophagus irregularis, with a cool view down an intercellular hypha. Stained with WGA-Alexafluor488, imaged by CLSM, false-coloured by depth in root 🔥
#microscopy #mycosci #fungi
New preprint by @kjohnsson.bsky.social lab!
A new split Halotag system with higher affinity of the complements + lower background. The system works with our SiR-CA & CPY-CA halotag ligands and enables STED imaging or FLIM multiplexing.
The short 14 aa tag Hpep enables easy cloning free CRISPR-KI.
A new split Halotag system with higher affinity of the complements + lower background. The system works with our SiR-CA & CPY-CA halotag ligands and enables STED imaging or FLIM multiplexing.
The short 14 aa tag Hpep enables easy cloning free CRISPR-KI.
![Live-cell confocal imaging of histone H2B type-2E-Hpep11 CRISPR KI cell lines after 2-hours labeling with the specified CA- ligand [100 nM]. Images were taken with optimal image acquisition parameter for each dye. Scale bar: 50 μm.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:uf4m7zlmcjlg2biwn4s2k32y/bafkreibozn5fz6sgeksqrz3vmy6uiydtnvwmb5my7r3xecko2erokryn3a)
![(a) Confocal laser scanning microscopy (CLSM) and STED images of mitochondria in U2OS cells coexpressing cpHaloΔ3 and TOM20-Hpep, either overexpressed or endogenously tagged. Scale bar: 1 μm. Pixel intensities scaled according to reference bar. (b) Representative CLSM, STED images of the CRISPR/Cas9 KI cells expressing TOM20 tagged with intact HaloTag (upper) or Hpep11 (bottom). (c) Intensity profiles along mitochondrial tubules (red and blue lines in b). Scale bar: 2 μm. (d) Representative CLSM and STED images of endogenous Hpep11-tagged clathrin with cpHaloΔ3 coexpression. Scale bar: 10 μm (overview) and 2 μm (magnification). (e) Representative CLSM, STED
images of endogenously tagged tubulin beta 4B with Hpep11. Scale bar: 10 μm (overview) and 2 μm (magnification). (f) Intensity profiles along tubulin filaments (red and blue lines in e) Means ± s.d. of the filament diameters were calculated as full width at half maximum (FWHM) from n=20 microtubule filaments, ≥ 2 images. A slight increase in cytosolic signal was noted in cells tagged with split-HaloTagat TUBB4B, compared to cells tagged with the full-length HaloTag, which may result from the presence of unbound but labeled cpHaloΔ3. All images were acquired after labeling with CA-SiR [100 nM] for one hour.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:uf4m7zlmcjlg2biwn4s2k32y/bafkreielty6fex37xy4caechbw5ebo3eypyiyvxxhkigeazd5gmy6tvlay)
June 17, 2025 at 11:09 AM
New preprint by @kjohnsson.bsky.social lab!
A new split Halotag system with higher affinity of the complements + lower background. The system works with our SiR-CA & CPY-CA halotag ligands and enables STED imaging or FLIM multiplexing.
The short 14 aa tag Hpep enables easy cloning free CRISPR-KI.
A new split Halotag system with higher affinity of the complements + lower background. The system works with our SiR-CA & CPY-CA halotag ligands and enables STED imaging or FLIM multiplexing.
The short 14 aa tag Hpep enables easy cloning free CRISPR-KI.
ASP 2026 was a success! I presented my current work developing an interactive identification key, redescriptions based on CLSM imaging and defining species groups. I also received the meritorious presentation award!
July 31, 2026 at 6:30 PM
ASP 2026 was a success! I presented my current work developing an interactive identification key, redescriptions based on CLSM imaging and defining species groups. I also received the meritorious presentation award!
They use Scanning Electron Microscopy (SEM) to examine the coating of rubbing mud on the surface of the baseball and Confocal Laser Scanning Microscopy (CLSM) with a finger-mimicking grip to show grains of sand being sheared off a coated baseball
November 16, 2024 at 6:35 PM
They use Scanning Electron Microscopy (SEM) to examine the coating of rubbing mud on the surface of the baseball and Confocal Laser Scanning Microscopy (CLSM) with a finger-mimicking grip to show grains of sand being sheared off a coated baseball
The NTNU team is working on introducing a biosensor for key plant hormone involved in #drought stress into various mutant backgrounds
1st mutant line is showing promising results under confocal laser scanning microscopy (CLSM)!
📸 Featured work by @sara-morghen.bsky.social #PlantScience #Science
1st mutant line is showing promising results under confocal laser scanning microscopy (CLSM)!
📸 Featured work by @sara-morghen.bsky.social #PlantScience #Science
February 21, 2025 at 4:40 PM
The NTNU team is working on introducing a biosensor for key plant hormone involved in #drought stress into various mutant backgrounds
1st mutant line is showing promising results under confocal laser scanning microscopy (CLSM)!
📸 Featured work by @sara-morghen.bsky.social #PlantScience #Science
1st mutant line is showing promising results under confocal laser scanning microscopy (CLSM)!
📸 Featured work by @sara-morghen.bsky.social #PlantScience #Science
Rune Hansen et al. reported that the substomatal cavity is a crucial check point where the devastating Septoria blotch (Zymoseptoria tritici) infection is stopped. Understanding this mechanism is key to future wheat immunity. Learn more: https://doi.org/10.1094/MPMI-11-24-0147-R
December 22, 2025 at 4:55 PM
Rune Hansen et al. reported that the substomatal cavity is a crucial check point where the devastating Septoria blotch (Zymoseptoria tritici) infection is stopped. Understanding this mechanism is key to future wheat immunity. Learn more: https://doi.org/10.1094/MPMI-11-24-0147-R
"Post any Drum N Bass, Jungle, Breakbeat, Dubstep song each day..."
#DrumNBreak #MusicChallenge | 31 of 31
Let's go out on a high note or at least on a high speed note.
Or what I mean to say, a note about a high number of BPMs:
"John Peel (Is Not Enough)" by CLSM (from "Hardcore Material", 2003)
#DrumNBreak #MusicChallenge | 31 of 31
Let's go out on a high note or at least on a high speed note.
Or what I mean to say, a note about a high number of BPMs:
"John Peel (Is Not Enough)" by CLSM (from "Hardcore Material", 2003)
John Peel (Not Enough) (Original Mix)
YouTube video by CLSM - Topic
music.youtube.com
July 30, 2025 at 10:09 PM
"Post any Drum N Bass, Jungle, Breakbeat, Dubstep song each day..."
#DrumNBreak #MusicChallenge | 31 of 31
Let's go out on a high note or at least on a high speed note.
Or what I mean to say, a note about a high number of BPMs:
"John Peel (Is Not Enough)" by CLSM (from "Hardcore Material", 2003)
#DrumNBreak #MusicChallenge | 31 of 31
Let's go out on a high note or at least on a high speed note.
Or what I mean to say, a note about a high number of BPMs:
"John Peel (Is Not Enough)" by CLSM (from "Hardcore Material", 2003)
#WochenendOhrwuermer
🤟🏼🎸🎶 Lieblingssongs 🎶❤️🔥🎻
Bad Company "Shooting Star" Live
www.youtube.com/watch?v=clsM...
🤟🏼🎸🎶 Lieblingssongs 🎶❤️🔥🎻
Bad Company "Shooting Star" Live
www.youtube.com/watch?v=clsM...
October 11, 2025 at 1:26 PM
#WochenendOhrwuermer
🤟🏼🎸🎶 Lieblingssongs 🎶❤️🔥🎻
Bad Company "Shooting Star" Live
www.youtube.com/watch?v=clsM...
🤟🏼🎸🎶 Lieblingssongs 🎶❤️🔥🎻
Bad Company "Shooting Star" Live
www.youtube.com/watch?v=clsM...
It´s #MicroscopyFriday. Here a nice example of Fusarium culmorum invading barley lemma epidermis. Propidium-iodide stains the plant cell wall, ink fungal surface strcutures and wheat germ agglutinin post-invasive fungal cell wall. Overlay of three detectors with CLSM transmission channel.
August 7, 2026 at 6:29 AM
It´s #MicroscopyFriday. Here a nice example of Fusarium culmorum invading barley lemma epidermis. Propidium-iodide stains the plant cell wall, ink fungal surface strcutures and wheat germ agglutinin post-invasive fungal cell wall. Overlay of three detectors with CLSM transmission channel.
I imaged the Rhynie chert plants using confocal laser scanning microscopy and scanning electron microscopy, two very high resolution imaging techniques that can be used to identify sub-micron features in these fossil plants. 4/
June 23, 2026 at 4:27 PM
I imaged the Rhynie chert plants using confocal laser scanning microscopy and scanning electron microscopy, two very high resolution imaging techniques that can be used to identify sub-micron features in these fossil plants. 4/
You’re my wonderwall… youtu.be/tP6Sf-ZPS6M?...
CLSM - Wonderwall Dub [FREE DOWNLOAD]
YouTube video by YosH
youtu.be
September 25, 2025 at 10:46 PM
You’re my wonderwall… youtu.be/tP6Sf-ZPS6M?...
New #OpenAccess work in #RESPhysEnt describes the morphological & biomechanical #adaptations of larval #mandibles in #Trichoptera
doi.org/10.1111/phen.70009
#Morphology #Biomechanics #Caddisflies #SEM #EDX #CLSM
@wybouw.bsky.social @tbuescher.bsky.social @tobybruce.bsky.social @wileyeco.bsky.social
doi.org/10.1111/phen.70009
#Morphology #Biomechanics #Caddisflies #SEM #EDX #CLSM
@wybouw.bsky.social @tbuescher.bsky.social @tobybruce.bsky.social @wileyeco.bsky.social
November 3, 2025 at 2:05 PM
New #OpenAccess work in #RESPhysEnt describes the morphological & biomechanical #adaptations of larval #mandibles in #Trichoptera
doi.org/10.1111/phen.70009
#Morphology #Biomechanics #Caddisflies #SEM #EDX #CLSM
@wybouw.bsky.social @tbuescher.bsky.social @tobybruce.bsky.social @wileyeco.bsky.social
doi.org/10.1111/phen.70009
#Morphology #Biomechanics #Caddisflies #SEM #EDX #CLSM
@wybouw.bsky.social @tbuescher.bsky.social @tobybruce.bsky.social @wileyeco.bsky.social
ちゃくえろclsmが好きなんだよおッッッ！！！
May 6, 2025 at 8:15 AM
ちゃくえろclsmが好きなんだよおッッッ！！！
🔴LIVE🔴
Pokémon: Colosseum
The Vibes Will Be So Good You Won't Realize I Don't Know What Steel Is Strong Against! Might Beat This Game Tonight...
www.twitch.tv/axle_bro
Pokémon: Colosseum
The Vibes Will Be So Good You Won't Realize I Don't Know What Steel Is Strong Against! Might Beat This Game Tonight...
www.twitch.tv/axle_bro
Axle_Bro - Twitch
🔴PKMN CLSM🔴 The Vibes Will Be So Good You Won't Realize I Don't Know What Steel Is Strong Against! Might Beat This Game Tonight
www.twitch.tv
July 11, 2025 at 12:06 AM
🔴LIVE🔴
Pokémon: Colosseum
The Vibes Will Be So Good You Won't Realize I Don't Know What Steel Is Strong Against! Might Beat This Game Tonight...
www.twitch.tv/axle_bro
Pokémon: Colosseum
The Vibes Will Be So Good You Won't Realize I Don't Know What Steel Is Strong Against! Might Beat This Game Tonight...
www.twitch.tv/axle_bro
🔎 DARWIN REVIEW 🔎
Mody et al. review the study of the cellular basis of plant morphogenesis with 3D digital organs 🔬🌱
🔗 doi.org/10.1093/jxb/...
#PlantScience 🧪 @kschneitz.bsky.social
Mody et al. review the study of the cellular basis of plant morphogenesis with 3D digital organs 🔬🌱
🔗 doi.org/10.1093/jxb/...
#PlantScience 🧪 @kschneitz.bsky.social
August 29, 2025 at 9:43 AM
🔎 DARWIN REVIEW 🔎
Mody et al. review the study of the cellular basis of plant morphogenesis with 3D digital organs 🔬🌱
🔗 doi.org/10.1093/jxb/...
#PlantScience 🧪 @kschneitz.bsky.social
Mody et al. review the study of the cellular basis of plant morphogenesis with 3D digital organs 🔬🌱
🔗 doi.org/10.1093/jxb/...
#PlantScience 🧪 @kschneitz.bsky.social
Purple Line tram/"LRT" construction - after they relocate infrastructure from underneath the right of way, they dig a trench about 1m-1.5m deep, lay tubes for using as cable conduits, then pour concrete (probably CLSM) on top of that. Slab track comes later, atop this concrete base
May 1, 2025 at 2:29 PM
Purple Line tram/"LRT" construction - after they relocate infrastructure from underneath the right of way, they dig a trench about 1m-1.5m deep, lay tubes for using as cable conduits, then pour concrete (probably CLSM) on top of that. Slab track comes later, atop this concrete base
But we found that the FCCs of Asteroxylon did have (submicron!) sieve pores, which in extant plants is involved in the movement of sugars through the phloem, suggesting this tissue did have a sugar conduction specialisation. This is the earliest example of sieve pores in the fossil record. 8/
June 23, 2026 at 4:27 PM
But we found that the FCCs of Asteroxylon did have (submicron!) sieve pores, which in extant plants is involved in the movement of sugars through the phloem, suggesting this tissue did have a sugar conduction specialisation. This is the earliest example of sieve pores in the fossil record. 8/