I'm speculating that one outcome of Prp2 remodeling is a shift in the U2 snRNA (esp. around U2 G20) that allows for Cwc15 peptide insertion. Thus, Cwc15 could "mark" a correctly (?) assembled and activated spliceosome catalytic center.
March 23, 2026 at 7:43 PM
I'm speculating that one outcome of Prp2 remodeling is a shift in the U2 snRNA (esp. around U2 G20) that allows for Cwc15 peptide insertion. Thus, Cwc15 could "mark" a correctly (?) assembled and activated spliceosome catalytic center.
For real splicing aficionados there is some bonus content in the supplemental. I noticed that whether or not the N-terminus of Cwc15 is inserted b/n the U2 and U6 snRNAs (or can be observed to be inserted) is correlated with Prp2 activity. Not inserted = before Prp2; Inserted = after.
March 23, 2026 at 7:43 PM
For real splicing aficionados there is some bonus content in the supplemental. I noticed that whether or not the N-terminus of Cwc15 is inserted b/n the U2 and U6 snRNAs (or can be observed to be inserted) is correlated with Prp2 activity. Not inserted = before Prp2; Inserted = after.
The take home story is that Cwc15 fine tune's the active site, likely explaining its impact on alternative splicing in organisms ranging from potatoes to cows.
March 23, 2026 at 7:43 PM
The take home story is that Cwc15 fine tune's the active site, likely explaining its impact on alternative splicing in organisms ranging from potatoes to cows.
We find that Cwc15 seems primarily to be involved in formation of the spliceosome active site responsible for 5' splice site cleavage as well as promoting that reaction itself. This isn't too surprising given the protein's location proximal to the U2/U6 disnRNA.
March 23, 2026 at 7:43 PM
We find that Cwc15 seems primarily to be involved in formation of the spliceosome active site responsible for 5' splice site cleavage as well as promoting that reaction itself. This isn't too surprising given the protein's location proximal to the U2/U6 disnRNA.
Happy to share our latest work on the previously uncharacterized splicing factor Cwc15! Importantly, all of the people who did this work are/were undergrads!
S. cerevisiae Cwc15p Tunes the Spliceosome Active Site for 5' Splice Site Cleavage
www.biorxiv.org/content/10.6...
S. cerevisiae Cwc15p Tunes the Spliceosome Active Site for 5' Splice Site Cleavage
www.biorxiv.org/content/10.6...
www.biorxiv.org
March 23, 2026 at 7:43 PM
Happy to share our latest work on the previously uncharacterized splicing factor Cwc15! Importantly, all of the people who did this work are/were undergrads!
S. cerevisiae Cwc15p Tunes the Spliceosome Active Site for 5' Splice Site Cleavage
www.biorxiv.org/content/10.6...
S. cerevisiae Cwc15p Tunes the Spliceosome Active Site for 5' Splice Site Cleavage
www.biorxiv.org/content/10.6...
