Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
October 2, 2025 at 5:11 PM
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
“research shows that cells actively reshape the endoplasmic reticulum or ER…Rather than remaining static the ER undergoes controlled remodeling as organisms grow older. The team discovered that this remodeling happens through a process called ER-phagy”
🧪 #CellSky
www.sciencedaily.com/releases/202...
🧪 #CellSky
www.sciencedaily.com/releases/202...
A hidden cellular process may drive aging and disease
As we age, our cells don’t just wear down—they reorganize. Researchers found that cells actively remodel a key structure called the endoplasmic reticulum, reducing protein-producing regions while pres...
www.sciencedaily.com
February 7, 2026 at 3:52 PM
“research shows that cells actively reshape the endoplasmic reticulum or ER…Rather than remaining static the ER undergoes controlled remodeling as organisms grow older. The team discovered that this remodeling happens through a process called ER-phagy”
🧪 #CellSky
www.sciencedaily.com/releases/202...
🧪 #CellSky
www.sciencedaily.com/releases/202...
December 10, 2024 at 4:07 AM
Classes start in a week (ah!) so re-sharing this!
Still soliciting suggestions for emerging model organisms for cell bio. "Model" can be interpreted broadly but looking for taxa that:
1) have an active research community
2) some combo of a genome, life cycle, culture.
#CellSky #AcademicSky
Still soliciting suggestions for emerging model organisms for cell bio. "Model" can be interpreted broadly but looking for taxa that:
1) have an active research community
2) some combo of a genome, life cycle, culture.
#CellSky #AcademicSky
Hi, #AcademiaSky! I'm a #newPI gearing up to teach a mid-level #CellBio course in spring 25. I'm implementing a final project where students work in small groups to make a poster on an emerging model organism in cell bio.
Please suggest potential taxa! Bonus points if you include a paper! (1/N)
Please suggest potential taxa! Bonus points if you include a paper! (1/N)
January 6, 2025 at 4:50 PM
Classes start in a week (ah!) so re-sharing this!
Still soliciting suggestions for emerging model organisms for cell bio. "Model" can be interpreted broadly but looking for taxa that:
1) have an active research community
2) some combo of a genome, life cycle, culture.
#CellSky #AcademicSky
Still soliciting suggestions for emerging model organisms for cell bio. "Model" can be interpreted broadly but looking for taxa that:
1) have an active research community
2) some combo of a genome, life cycle, culture.
#CellSky #AcademicSky