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CRISPR-Cas技术在食品鉴定中的应用版图：一项文献计量学综述
CRISPR-Cas技术在食品鉴定中的应用版图：一项文献计量学综述
食品
食品
CRISPR-Cas技术在食品鉴定中的应用版图：一项文献计量学综述
www.ebiotrade.com
October 3, 2026 at 3:40 AM
食品
CRISPR-Cas技术在食品鉴定中的应用版图：一项文献计量学综述
CRISPR-Cas技术在食品鉴定中的应用版图：一项文献计量学综述
Some bacteria use the CRISPR/Cas systems as transcriptional repressors of other anti-phage systems, such that when CRISPR interfered with, gene expression of repressed anti-phage systems goes up www.nature.com/articles/s41...
CRISPR–Cas regulates expression of embedded anti-phage defence systems - Nature
CRISPR–Cas systems transcriptionally tune diverse innate defences using CRISPR RNA-like guides to balance antiviral protection with fitness, and hyperactivate these defences when compromised, establis...
www.nature.com
July 22, 2026 at 5:21 PM
Some bacteria use the CRISPR/Cas systems as transcriptional repressors of other anti-phage systems, such that when CRISPR interfered with, gene expression of repressed anti-phage systems goes up www.nature.com/articles/s41...
Ever wondered why some bacteria have multiple CRISPR-Cas systems? Our new study led by Leah Smith shows how type I CRISPR systems can promote the acquisition and retention of new spacers into a co-occuring type III system. www.sciencedirect.com/science/arti...
Type I CRISPR-Cas immunity primes type III spacer acquisition
CRISPR-Cas systems are diverse, with microbes harboring multiple classes and subtypes. Type I DNA-targeting and type III RNA-targeting systems often c…
www.sciencedirect.com
August 18, 2025 at 3:06 PM
Ever wondered why some bacteria have multiple CRISPR-Cas systems? Our new study led by Leah Smith shows how type I CRISPR systems can promote the acquisition and retention of new spacers into a co-occuring type III system. www.sciencedirect.com/science/arti...
Out now in Science: Two papers from our lab reveal VIPR, an RNA-guided DNA-targeting system in bacteriophages, providing clues to the origins of CRISPR
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Congrats to Peter Yoon, @kenjmloi.bsky.social @terryzzy.bsky.social, T.Docter & team
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Congrats to Peter Yoon, @kenjmloi.bsky.social @terryzzy.bsky.social, T.Docter & team
A noncontiguous code for RNA-guided DNA recognition at the origin of CRISPR-Cas
CRISPR-Cas provides RNA-mediated adaptive immunity, but how its first RNA-guided effector arose is unclear. In this study, we report the discovery of Viral Interference Programmable Repeat (VIPR) syst...
www.science.org
September 19, 2026 at 1:12 AM
Out now in Science: Two papers from our lab reveal VIPR, an RNA-guided DNA-targeting system in bacteriophages, providing clues to the origins of CRISPR
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Congrats to Peter Yoon, @kenjmloi.bsky.social @terryzzy.bsky.social, T.Docter & team
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Congrats to Peter Yoon, @kenjmloi.bsky.social @terryzzy.bsky.social, T.Docter & team
CRISPR-Cas–mediated heritable chromosome fusions in Arabidopsis | Science www.science.org/doi/10.1126/...
Very nice work from Holger Puchta & colleagues
Very nice work from Holger Puchta & colleagues
CRISPR-Cas–mediated heritable chromosome fusions in Arabidopsis
The genome of Arabidopsis thaliana consists of 10 chromosomes. By inducing CRISPR-Cas–mediated breaks at subcentromeric and subtelomeric sequences, we fused entire chromosome arms, obtaining two eight...
www.science.org
November 20, 2025 at 8:47 PM
CRISPR-Cas–mediated heritable chromosome fusions in Arabidopsis | Science www.science.org/doi/10.1126/...
Very nice work from Holger Puchta & colleagues
Very nice work from Holger Puchta & colleagues
ICYMI: CRISPR–Cas therapies targeting bacteria
CRISPR–Cas therapies targeting bacteria
Nature Reviews Bioengineering, Published online: 13 May 2025; doi:10.1038/s44222-025-00311-8CRISPR–Cas tools can be designed to kill or modify specific bacteria. This Review explores the engineering of CRISPR–Cas tools and corresponding delivery strategies for the treatment of bacterial infections and modification of the microbiome.
www.nature.com
May 15, 2025 at 6:53 AM
After 5 years of waiting, the new #CRISPR classification by Makarova et al. is out @natmicrobiol.nature.com www.nature.com/articles/s41...
An updated evolutionary classification of CRISPR–Cas systems including rare variants - Nature Microbiology
An exploration of previously undescribed variants from the long tail of the CRISPR–Cas distribution.
www.nature.com
November 6, 2025 at 1:49 PM
After 5 years of waiting, the new #CRISPR classification by Makarova et al. is out @natmicrobiol.nature.com www.nature.com/articles/s41...
New preprint!
Ever wondered why only a fraction of genomes encode CRISPR immunity? 🧬 🦠
Turns out CRISPR is rarely beneficial against virulent phages, being most beneficial against those for which resistance mutations are rare!
An epic effort by Rosanna Wright
www.biorxiv.org/content/10.1...
Ever wondered why only a fraction of genomes encode CRISPR immunity? 🧬 🦠
Turns out CRISPR is rarely beneficial against virulent phages, being most beneficial against those for which resistance mutations are rare!
An epic effort by Rosanna Wright
www.biorxiv.org/content/10.1...
Resistance mutation supply modulates the benefit of CRISPR immunity against virulent phages
Only a fraction of bacterial genomes encode CRISPR-Cas systems but the selective causes of this variation are unexplained. How naturally virulent bacteriophages (phages) select for CRISPR immunity has...
www.biorxiv.org
October 6, 2025 at 6:27 AM
New preprint!
Ever wondered why only a fraction of genomes encode CRISPR immunity? 🧬 🦠
Turns out CRISPR is rarely beneficial against virulent phages, being most beneficial against those for which resistance mutations are rare!
An epic effort by Rosanna Wright
www.biorxiv.org/content/10.1...
Ever wondered why only a fraction of genomes encode CRISPR immunity? 🧬 🦠
Turns out CRISPR is rarely beneficial against virulent phages, being most beneficial against those for which resistance mutations are rare!
An epic effort by Rosanna Wright
www.biorxiv.org/content/10.1...
"Forscher haben HIV aus Zellen eliminiert, was Hoffnungen auf eine Heilung weckt.
Mit Hilfe [von] Crispr/Cas, das 2020 mit dem Nobelpreis ausgezeichnet wurde, konnten Wissenschaftler die HIV-DNA gezielt angreifen und alle Spuren des Virus aus infizierten Zellen entfernen."
(The Independent)
Mit Hilfe [von] Crispr/Cas, das 2020 mit dem Nobelpreis ausgezeichnet wurde, konnten Wissenschaftler die HIV-DNA gezielt angreifen und alle Spuren des Virus aus infizierten Zellen entfernen."
(The Independent)
HIV cure breakthrough as virus eliminated from cells in laboratory
It is thanks to a gene-editing tool known as Crispr-Cas, which won the Nobel Prize in 2020
www.independent.co.uk
March 26, 2024 at 6:43 PM
"Forscher haben HIV aus Zellen eliminiert, was Hoffnungen auf eine Heilung weckt.
Mit Hilfe [von] Crispr/Cas, das 2020 mit dem Nobelpreis ausgezeichnet wurde, konnten Wissenschaftler die HIV-DNA gezielt angreifen und alle Spuren des Virus aus infizierten Zellen entfernen."
(The Independent)
Mit Hilfe [von] Crispr/Cas, das 2020 mit dem Nobelpreis ausgezeichnet wurde, konnten Wissenschaftler die HIV-DNA gezielt angreifen und alle Spuren des Virus aus infizierten Zellen entfernen."
(The Independent)
Crispr-Cas, unlike AI, is living up to its hype in spades.
April 14, 2024 at 11:28 PM
Crispr-Cas, unlike AI, is living up to its hype in spades.
CRISPR–Cas applications in agriculture and plant research
www.nature.com/articles/s41...
www.nature.com/articles/s41...
CRISPR–Cas applications in agriculture and plant research - Nature Reviews Molecular Cell Biology
CRISPR–Cas-mediated genome, epigenome and transcriptome engineering in crops is crucial as the human population grows and climate deteriorates. This Review discusses new CRISPR–Cas tools for precise e...
www.nature.com
March 9, 2025 at 12:04 PM
CRISPR–Cas applications in agriculture and plant research
www.nature.com/articles/s41...
www.nature.com/articles/s41...
And now we have Arabidopsis plants with 8 chromosomes instead of 10 and no obvious phenotypic differences, this week in @science.org
#PlantScience
Paper here: www.science.org/doi/10.1126/...
Perspective here:
www.science.org/doi/10.1126/...
#PlantScience
Paper here: www.science.org/doi/10.1126/...
Perspective here:
www.science.org/doi/10.1126/...
CRISPR-Cas–mediated heritable chromosome fusions in Arabidopsis
The genome of Arabidopsis thaliana consists of 10 chromosomes. By inducing CRISPR-Cas–mediated breaks at subcentromeric and subtelomeric sequences, we fused entire chromosome arms, obtaining two eight...
www.science.org
November 21, 2025 at 10:20 AM
And now we have Arabidopsis plants with 8 chromosomes instead of 10 and no obvious phenotypic differences, this week in @science.org
#PlantScience
Paper here: www.science.org/doi/10.1126/...
Perspective here:
www.science.org/doi/10.1126/...
#PlantScience
Paper here: www.science.org/doi/10.1126/...
Perspective here:
www.science.org/doi/10.1126/...
@peterfineran.bsky.social and colleagues develop Tn-seq for phages by leveraging anti-CRISPR (Acr) as a positive
selectable marker in the presence of CRISPR-Cas counter-selection, and identify essential genes in phage from non model orgs
www.biorxiv.org/content/10.6...
selectable marker in the presence of CRISPR-Cas counter-selection, and identify essential genes in phage from non model orgs
www.biorxiv.org/content/10.6...
December 20, 2025 at 11:34 PM
@peterfineran.bsky.social and colleagues develop Tn-seq for phages by leveraging anti-CRISPR (Acr) as a positive
selectable marker in the presence of CRISPR-Cas counter-selection, and identify essential genes in phage from non model orgs
www.biorxiv.org/content/10.6...
selectable marker in the presence of CRISPR-Cas counter-selection, and identify essential genes in phage from non model orgs
www.biorxiv.org/content/10.6...
Customizing CRISPR–Cas PAM specificity with protein language models - @thisismadani.bsky.social go.nature.com/4a4G9Zy
Customizing CRISPR–Cas PAM specificity with protein language models - Nature Biotechnology
The protospacer-adjacent motif specificity of several Cas enzymes is customized using protein language models.
go.nature.com
February 2, 2026 at 4:07 PM
Customizing CRISPR–Cas PAM specificity with protein language models - @thisismadani.bsky.social go.nature.com/4a4G9Zy
Genome editing in brown algae! 🧬🪸🌿 Now out in Cell Reports Methods!
Excited to share this highly efficient, transgene-free CRISPR–Cas genome editing protocol for brown algae, requiring no cloning and no specialized equipment.
doi.org/10.1016/j.cr...
#CRISPR #BrownAlgae
@mpi-bio-fml.bsky.social
Excited to share this highly efficient, transgene-free CRISPR–Cas genome editing protocol for brown algae, requiring no cloning and no specialized equipment.
doi.org/10.1016/j.cr...
#CRISPR #BrownAlgae
@mpi-bio-fml.bsky.social
January 2, 2026 at 2:20 PM
Genome editing in brown algae! 🧬🪸🌿 Now out in Cell Reports Methods!
Excited to share this highly efficient, transgene-free CRISPR–Cas genome editing protocol for brown algae, requiring no cloning and no specialized equipment.
doi.org/10.1016/j.cr...
#CRISPR #BrownAlgae
@mpi-bio-fml.bsky.social
Excited to share this highly efficient, transgene-free CRISPR–Cas genome editing protocol for brown algae, requiring no cloning and no specialized equipment.
doi.org/10.1016/j.cr...
#CRISPR #BrownAlgae
@mpi-bio-fml.bsky.social
Out now in @plosbiology.org : our big joint effort on the role of #CRISPR in plasmid competition. Read on for a really fun (I’m biased ok) analysis of how a defence system has new selective pressures when it’s mobile
journals.plos.org/plosbiology/... 1/6
journals.plos.org/plosbiology/... 1/6
February 20, 2026 at 5:50 PM
Out now in @plosbiology.org : our big joint effort on the role of #CRISPR in plasmid competition. Read on for a really fun (I’m biased ok) analysis of how a defence system has new selective pressures when it’s mobile
journals.plos.org/plosbiology/... 1/6
journals.plos.org/plosbiology/... 1/6
📜 CRISPR–Cas applications in agriculture and plant research
🧑🔬 Aytug Tuncel, Changtian Pan, Yiping Qi, et al.
📔 @natrevmcb.bsky.social
🔗 www.nature.com/articles/s41...
#️⃣ #PlantScience #PlantNGTs #PlantCRISPR #Agriculture #PlantGenomics #PlantGenomeEditing
🧑🔬 Aytug Tuncel, Changtian Pan, Yiping Qi, et al.
📔 @natrevmcb.bsky.social
🔗 www.nature.com/articles/s41...
#️⃣ #PlantScience #PlantNGTs #PlantCRISPR #Agriculture #PlantGenomics #PlantGenomeEditing
CRISPR–Cas applications in agriculture and plant research - Nature Reviews Molecular Cell Biology
CRISPR–Cas-mediated genome, epigenome and transcriptome engineering in crops is crucial as the human population grows and climate deteriorates. This Review discusses new CRISPR–Cas tools for precise e...
www.nature.com
March 14, 2025 at 7:40 AM
📜 CRISPR–Cas applications in agriculture and plant research
🧑🔬 Aytug Tuncel, Changtian Pan, Yiping Qi, et al.
📔 @natrevmcb.bsky.social
🔗 www.nature.com/articles/s41...
#️⃣ #PlantScience #PlantNGTs #PlantCRISPR #Agriculture #PlantGenomics #PlantGenomeEditing
🧑🔬 Aytug Tuncel, Changtian Pan, Yiping Qi, et al.
📔 @natrevmcb.bsky.social
🔗 www.nature.com/articles/s41...
#️⃣ #PlantScience #PlantNGTs #PlantCRISPR #Agriculture #PlantGenomics #PlantGenomeEditing
Nature research paper: Design of highly functional genome editors by modelling CRISPR–Cas sequences
go.nature.com/3UAYBBB
go.nature.com/3UAYBBB
Design of highly functional genome editors by modelling CRISPR–Cas sequences - Nature
Gene editors designed using artificial intelligence can undertake precision editing of the human genome.
go.nature.com
July 30, 2025 at 6:50 PM
Nature research paper: Design of highly functional genome editors by modelling CRISPR–Cas sequences
go.nature.com/3UAYBBB
go.nature.com/3UAYBBB
Can CRISPR-Cas systems target any phage? Yes, no, it depends? What does it depend on? The second paper from my PhD came out today in a special issue of Phil Trans B on the ecology and evolution of bacterial immune systems
doi.org/10.1098/rstb...
doi.org/10.1098/rstb...
Phage susceptibility to a minimal, modular synthetic CRISPR-Cas system in Pseudomonas aeruginosa is nutrient dependent | Philosophical Transactions of the Royal Society B: Biological Sciences
CRISPR-Cas systems can provide adaptive, heritable immunity to their prokaryotic hosts
against invading genetic material such as phages. It is clear that the importance
of acquiring CRISPR-Cas immunit...
doi.org
September 4, 2025 at 11:32 AM
Can CRISPR-Cas systems target any phage? Yes, no, it depends? What does it depend on? The second paper from my PhD came out today in a special issue of Phil Trans B on the ecology and evolution of bacterial immune systems
doi.org/10.1098/rstb...
doi.org/10.1098/rstb...
#phagesky #microsky #phage defence #microbiome
CRISPR-cas activated by anoxia and regulated by Fnr in Citrobacter rodentium
www.nature.com/articles/s41...
CRISPR-cas activated by anoxia and regulated by Fnr in Citrobacter rodentium
www.nature.com/articles/s41...
Anoxia activates CRISPR–Cas immunity in the mouse intestine - Nature Microbiology
The Citrobacter rodentium CRISPR–Cas system is activated by the oxygen-responsive transcriptional regulator Fnr in the anoxic environment of the mouse gut.
www.nature.com
October 30, 2025 at 8:05 AM
#phagesky #microsky #phage defence #microbiome
CRISPR-cas activated by anoxia and regulated by Fnr in Citrobacter rodentium
www.nature.com/articles/s41...
CRISPR-cas activated by anoxia and regulated by Fnr in Citrobacter rodentium
www.nature.com/articles/s41...
CRISPR-Cas is beneficial in plasmid competition, but limited by competitor toxin–antitoxin activity when horizontally transferred | PLOS Biology https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3003658
CRISPR-Cas is beneficial in plasmid competition, but limited by competitor toxin–antitoxin activity when horizontally transferred
Plasmids use immune systems like CRISPR-Cas to compete with other plasmids, but it is unclear when these systems provide a selective advantage. This study shows that CRISPR-Cas benefits resident plasmids but is constrained by toxin-antitoxin systems after horizontal transfer.
journals.plos.org
February 20, 2026 at 5:00 AM
CRISPR-Cas is beneficial in plasmid competition, but limited by competitor toxin–antitoxin activity when horizontally transferred | PLOS Biology https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3003658
@katharinasures.bsky.social @probstlab.bsky.social et al. analysed CRISPR-Cas systems of metagenome-assembled genomes from two subsurface environments, shedding new light on the diversity of CRISPR spacers in natural microbial communities.
🔗 doi.org/10.1093/gbe/evaf201
#genome #evolution #CRISPR
🔗 doi.org/10.1093/gbe/evaf201
#genome #evolution #CRISPR
Acquisition of Spacers from Foreign Prokaryotic Genomes by CRISPR-Cas Systems in Natural Environments
Abstract. Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems of bacteria and archaea provide immunities
doi.org
November 18, 2025 at 10:53 AM
@katharinasures.bsky.social @probstlab.bsky.social et al. analysed CRISPR-Cas systems of metagenome-assembled genomes from two subsurface environments, shedding new light on the diversity of CRISPR spacers in natural microbial communities.
🔗 doi.org/10.1093/gbe/evaf201
#genome #evolution #CRISPR
🔗 doi.org/10.1093/gbe/evaf201
#genome #evolution #CRISPR
New paper from our group - megaphages, compact CRISPR-Cas effectors, and more from landfill phage, led by the talented Dr. Nikhil George (not on bsky). rdcu.be/ePo8o
Discarded diversity: novel megaphages, auxiliary metabolic genes, and virally encoded CRISPR-Cas systems in landfills
link.springer.com
November 11, 2025 at 6:38 PM
New paper from our group - megaphages, compact CRISPR-Cas effectors, and more from landfill phage, led by the talented Dr. Nikhil George (not on bsky). rdcu.be/ePo8o