DGD Volume 68, Issue 1 was released! Cover Photograph: Section of ID3 of a chicken hindlimb from an HH33 embryo stained for ROS (DCFHDA, red), nuclei (Hoechst, blue), active lysosomes (LysoTracker, green), and actin (Phalloidin, cyan)
January 16, 2026 at 8:55 AM
DGD Volume 68, Issue 1 was released! Cover Photograph: Section of ID3 of a chicken hindlimb from an HH33 embryo stained for ROS (DCFHDA, red), nuclei (Hoechst, blue), active lysosomes (LysoTracker, green), and actin (Phalloidin, cyan)
❓Tech Q&A | Use 10 μM for 5-30 min (commonly 20 min) at 37°C.
🧪Dilute 1:1000 in serum-free medium, cover #cells, wash 3× with serum-free medium.
🔃Order matters: short (≤2 h) load probe➡️stimulate; long (≥6 h) stimulate➡️load #probe.
✅Include a positive control (e.g. #CCCP).
#ROS #LabTips #DCFHDA
🧪Dilute 1:1000 in serum-free medium, cover #cells, wash 3× with serum-free medium.
🔃Order matters: short (≤2 h) load probe➡️stimulate; long (≥6 h) stimulate➡️load #probe.
✅Include a positive control (e.g. #CCCP).
#ROS #LabTips #DCFHDA
February 10, 2026 at 5:05 PM