We present VIPR, a phage-encoded RNA-guided system that recognizes DNA with a noncontiguous code unlike CRISPR.
Tiny, programmable, possibly ancestral to CRISPR immunity; VIPR is built around a striking RNA-DNA-DNA triplex.
Tiny, programmable, possibly ancestral to CRISPR immunity; VIPR is built around a striking RNA-DNA-DNA triplex.
Excited to share our discovery of a new programmable RNA-guided DNA-targeting system hiding inside bacteriophages that predates CRISPR.
We call it VIPR (Viral Interference Programmable Repeat), and it uses an entirely new logic to find its targets.
Thread + link below.
We call it VIPR (Viral Interference Programmable Repeat), and it uses an entirely new logic to find its targets.
Thread + link below.
April 27, 2026 at 9:34 PM
We present VIPR, a phage-encoded RNA-guided system that recognizes DNA with a noncontiguous code unlike CRISPR.
Tiny, programmable, possibly ancestral to CRISPR immunity; VIPR is built around a striking RNA-DNA-DNA triplex.
Tiny, programmable, possibly ancestral to CRISPR immunity; VIPR is built around a striking RNA-DNA-DNA triplex.
Out now in Science: Two papers from our lab reveal VIPR, an RNA-guided DNA-targeting system in bacteriophages, providing clues to the origins of CRISPR
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Congrats to Peter Yoon, @kenjmloi.bsky.social @terryzzy.bsky.social, T.Docter & team
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Congrats to Peter Yoon, @kenjmloi.bsky.social @terryzzy.bsky.social, T.Docter & team
A noncontiguous code for RNA-guided DNA recognition at the origin of CRISPR-Cas
CRISPR-Cas provides RNA-mediated adaptive immunity, but how its first RNA-guided effector arose is unclear. In this study, we report the discovery of Viral Interference Programmable Repeat (VIPR) syst...
www.science.org
September 19, 2026 at 1:12 AM
Out now in Science: Two papers from our lab reveal VIPR, an RNA-guided DNA-targeting system in bacteriophages, providing clues to the origins of CRISPR
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Congrats to Peter Yoon, @kenjmloi.bsky.social @terryzzy.bsky.social, T.Docter & team
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Congrats to Peter Yoon, @kenjmloi.bsky.social @terryzzy.bsky.social, T.Docter & team
Excited to share our discovery of a new programmable RNA-guided DNA-targeting system hiding inside bacteriophages that predates CRISPR.
We call it VIPR (Viral Interference Programmable Repeat), and it uses an entirely new logic to find its targets.
Thread + link below.
We call it VIPR (Viral Interference Programmable Repeat), and it uses an entirely new logic to find its targets.
Thread + link below.
April 27, 2026 at 9:32 PM
Excited to share our discovery of a new programmable RNA-guided DNA-targeting system hiding inside bacteriophages that predates CRISPR.
We call it VIPR (Viral Interference Programmable Repeat), and it uses an entirely new logic to find its targets.
Thread + link below.
We call it VIPR (Viral Interference Programmable Repeat), and it uses an entirely new logic to find its targets.
Thread + link below.
Nature research paper: DNA-guided transcription factor interactions extend human gene regulatory code
https://go.nature.com/4j13XRc
https://go.nature.com/4j13XRc
DNA-guided transcription factor interactions extend human gene regulatory code - Nature
A large-scale analysis of DNA-bound transcription factors (TFs) shows how the presence of DNA markedly affects the landscape of TF interactions, and identifies composite motifs that are recognized by complexes of TFs rather than by individual ones.
go.nature.com
April 9, 2025 at 5:24 PM
Nature research paper: DNA-guided transcription factor interactions extend human gene regulatory code
https://go.nature.com/4j13XRc
https://go.nature.com/4j13XRc
DNA-guided CRISPR–Cas12 for cellular RNA targeting - @ufresearch.bsky.social go.nature.com/49SP29d
DNA-guided CRISPR–Cas12 for cellular RNA targeting - Nature Biotechnology
ΨDNA guides are used with Cas12 for precise, programmable targeting of cellular RNA.
go.nature.com
May 15, 2026 at 3:07 PM
DNA-guided CRISPR–Cas12 for cellular RNA targeting - @ufresearch.bsky.social go.nature.com/49SP29d
It's an interesting phenomenon that some of the deepest questions about how life works have become what looks like impossibly obscure molecular biology stuck right at the back of Nature, which will never get covered by the science media. Like this. /1
www.nature.com/articles/s41...
www.nature.com/articles/s41...
DNA-guided transcription factor interactions extend human gene regulatory code - Nature
A large-scale analysis of DNA-bound transcription factors (TFs) shows how the presence of DNA markedly affects the landscape of TF interactions, and identifies composite motifs that are recognized by ...
www.nature.com
May 31, 2025 at 6:06 PM
It's an interesting phenomenon that some of the deepest questions about how life works have become what looks like impossibly obscure molecular biology stuck right at the back of Nature, which will never get covered by the science media. Like this. /1
www.nature.com/articles/s41...
www.nature.com/articles/s41...
we recently found some really neat RNA-guided DNA-cutting systems in phages. Despite remarkable similarities to CRISPR systems, including encoding guide RNAs in arrays, they appear entirely evolutionarily distinct (but definitely related to snoRNAs 🤓)
We decided to call them TIGR-Tas systems 🐯
We decided to call them TIGR-Tas systems 🐯
March 1, 2025 at 12:18 AM
we recently found some really neat RNA-guided DNA-cutting systems in phages. Despite remarkable similarities to CRISPR systems, including encoding guide RNAs in arrays, they appear entirely evolutionarily distinct (but definitely related to snoRNAs 🤓)
We decided to call them TIGR-Tas systems 🐯
We decided to call them TIGR-Tas systems 🐯
🧬🌉Online @science.org Programmable genome editing in human cells using RNA-guided bridge recombinases | Science www.science.org/doi/10.1126/...
Programmable genome editing in human cells using RNA-guided bridge recombinases
Site-specific insertion of gene-sized DNA fragments remains an unmet need in the genome editing field. IS110-family serine recombinases have recently been shown to mediate programmable DNA recombinati...
www.science.org
February 6, 2026 at 12:12 AM
🧬🌉Online @science.org Programmable genome editing in human cells using RNA-guided bridge recombinases | Science www.science.org/doi/10.1126/...
TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses | Science https://www.science.org/doi/10.1126/science.adv9789
TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses
RNA-guided systems provide remarkable versatility, enabling diverse biological functions. Through iterative structural and sequence homology-based mining starting with a guide RNA-interaction domain of Cas9, we identified a family of RNA-guided DNA-...
www.science.org
February 28, 2025 at 12:46 AM
TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses | Science https://www.science.org/doi/10.1126/science.adv9789
A tour de force study from Taipale&Yin labs. It expands the vocabulary of the Regulatory Code by adding 1131 TF:TF composite motifs that are different from the individual TF motifs. The new composite motifs are enriched in cell-type specific elements and active in vivo
www.nature.com/articles/s41...
www.nature.com/articles/s41...
DNA-guided transcription factor interactions extend human gene regulatory code - Nature
A large-scale analysis of DNA-bound transcription factors (TFs) shows how the presence of DNA markedly affects the landscape of TF interactions, and identifies composite motifs that are recognized by ...
www.nature.com
April 9, 2025 at 4:51 PM
A tour de force study from Taipale&Yin labs. It expands the vocabulary of the Regulatory Code by adding 1131 TF:TF composite motifs that are different from the individual TF motifs. The new composite motifs are enriched in cell-type specific elements and active in vivo
www.nature.com/articles/s41...
www.nature.com/articles/s41...
13 years ago, the revolutionary genetic tool #CRISPR/Cas9 was published in Science, work that Emmanuelle Charpentier and Jennifer Doudna won a #NobelPrize for in 2020.
On #WorldCRISPRDay, read the landmark paper that describes the discovery: https://scim.ag/42JHuSN
On #WorldCRISPRDay, read the landmark paper that describes the discovery: https://scim.ag/42JHuSN
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
A prokaryotic RNA–directed targeting system can be designed to cleave any DNA sequence.
scim.ag
October 20, 2025 at 7:33 PM
13 years ago, the revolutionary genetic tool #CRISPR/Cas9 was published in Science, work that Emmanuelle Charpentier and Jennifer Doudna won a #NobelPrize for in 2020.
On #WorldCRISPRDay, read the landmark paper that describes the discovery: https://scim.ag/42JHuSN
On #WorldCRISPRDay, read the landmark paper that describes the discovery: https://scim.ag/42JHuSN
🧬🌉Online @science.org Megabase-scale human #genome rearrangement with programmable bridge recombinases | Science www.science.org/doi/10.1126/...
Megabase-scale human genome rearrangement with programmable bridge recombinases
Bridge recombinases are naturally occurring RNA-guided DNA recombinases that we previously demonstrated can programmably insert, excise, and invert DNA in vitro and in Escherichia coli. In this study,...
www.science.org
September 29, 2025 at 10:01 PM
🧬🌉Online @science.org Megabase-scale human #genome rearrangement with programmable bridge recombinases | Science www.science.org/doi/10.1126/...
DNA-guided CRISPR–Cas12a effectors for programmable RNA recognition and cleavage go.nature.com/3P1TxHh
DNA-guided CRISPR–Cas12a effectors for programmable RNA recognition and cleavage - Nature Biotechnology
Synthetic DNA guides (crDNA) reprogram Cas12a nucleases for RNA targeting.
go.nature.com
May 4, 2026 at 1:48 PM
DNA-guided CRISPR–Cas12a effectors for programmable RNA recognition and cleavage go.nature.com/3P1TxHh
New preprint from the Sternberg lab!
We used pooled oligonucleotide library mutagenesis + high-throughput sequencing to systematically dissect the molecular determinants of IStron transposition, RNA-guided DNA cleavage, and self-splicing.
www.biorxiv.org/content/10.6...
We used pooled oligonucleotide library mutagenesis + high-throughput sequencing to systematically dissect the molecular determinants of IStron transposition, RNA-guided DNA cleavage, and self-splicing.
www.biorxiv.org/content/10.6...
March 16, 2026 at 6:59 PM
New preprint from the Sternberg lab!
We used pooled oligonucleotide library mutagenesis + high-throughput sequencing to systematically dissect the molecular determinants of IStron transposition, RNA-guided DNA cleavage, and self-splicing.
www.biorxiv.org/content/10.6...
We used pooled oligonucleotide library mutagenesis + high-throughput sequencing to systematically dissect the molecular determinants of IStron transposition, RNA-guided DNA cleavage, and self-splicing.
www.biorxiv.org/content/10.6...
DNA‑guided CRISPR suggests a new direction for RNA editing #NBTintheNews www.genengnews.com/topics/genom...
DNA‑Guided CRISPR Suggests a New Direction for RNA Editing
The new CRISPR platform, called ΨDNA, reprograms Cas12 nucleases to recognize and act on RNA using a DNA-based guide scaffold.
www.genengnews.com
May 19, 2026 at 1:27 PM
DNA‑guided CRISPR suggests a new direction for RNA editing #NBTintheNews www.genengnews.com/topics/genom...
Analysis and control of untemplated DNA polymerase activity for guided synthesis of kilobase-scale DNA sequences
doi.org/10.1038/s414...
doi.org/10.1038/s414...
March 10, 2026 at 7:24 AM
Analysis and control of untemplated DNA polymerase activity for guided synthesis of kilobase-scale DNA sequences
doi.org/10.1038/s414...
doi.org/10.1038/s414...
TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses www.science.org/doi/abs/10.1... #jcampubs
TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses
RNA-guided systems provide remarkable versatility, enabling diverse biological functions. Through iterative structural and sequence homology-based mining starting with a guide RNA-interaction domain o...
www.science.org
February 28, 2025 at 3:11 PM
TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses www.science.org/doi/abs/10.1... #jcampubs
🚀 Thrilled to share our latest work from Zhang Lab, now published in Science (www.science.org/doi/10.1126/...)
We introduce TIGR-Tas, a PAM-free, compact, modular RNA-guided DNA-targeting system in prokaryotes & their viruses.
More details coming soon 👀
#TIGR #RNA #GeneEditing #CRISPR
We introduce TIGR-Tas, a PAM-free, compact, modular RNA-guided DNA-targeting system in prokaryotes & their viruses.
More details coming soon 👀
#TIGR #RNA #GeneEditing #CRISPR
February 28, 2025 at 10:41 PM
🚀 Thrilled to share our latest work from Zhang Lab, now published in Science (www.science.org/doi/10.1126/...)
We introduce TIGR-Tas, a PAM-free, compact, modular RNA-guided DNA-targeting system in prokaryotes & their viruses.
More details coming soon 👀
#TIGR #RNA #GeneEditing #CRISPR
We introduce TIGR-Tas, a PAM-free, compact, modular RNA-guided DNA-targeting system in prokaryotes & their viruses.
More details coming soon 👀
#TIGR #RNA #GeneEditing #CRISPR
Wait... what !? 😱
A new family of RNA-guided systems in prokaryotes ! Mind-blowing !
A new family of RNA-guided systems in prokaryotes ! Mind-blowing !
TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses
RNA-guided systems provide remarkable versatility, enabling diverse biological functions. Through iterative structural and sequence homology-based mining starting with a guide RNA-interaction domain o...
www.science.org
February 28, 2025 at 8:47 AM
Wait... what !? 😱
A new family of RNA-guided systems in prokaryotes ! Mind-blowing !
A new family of RNA-guided systems in prokaryotes ! Mind-blowing !
I dream of a day when my politicians are guided by the pathological, all-consuming, visceral, seething fucking hatred for MAGA that seeps into my DNA & radiates through every fiber of my goddamn being. Republicans aren't your colleagues. They're your enemies. Fucking act like it.
January 11, 2025 at 9:42 PM
I dream of a day when my politicians are guided by the pathological, all-consuming, visceral, seething fucking hatred for MAGA that seeps into my DNA & radiates through every fiber of my goddamn being. Republicans aren't your colleagues. They're your enemies. Fucking act like it.
New preprint 👉Doudna x Bryant x Jacobsen x Savage collaboration!
Work led by @zehanzhou.bsky.social, I. Saffarian-Deemyad, @honglue.bsky.social, T. Weiss
We dissect how stepwise DNA unwinding gates TnpB genome editing, revealing how unwound DNA states enhance cleavage
www.biorxiv.org/content/10.6...
Work led by @zehanzhou.bsky.social, I. Saffarian-Deemyad, @honglue.bsky.social, T. Weiss
We dissect how stepwise DNA unwinding gates TnpB genome editing, revealing how unwound DNA states enhance cleavage
www.biorxiv.org/content/10.6...
Stepwise DNA unwinding gates TnpB genome-editing activity
TnpB is a compact RNA-guided endonuclease and evolutionary ancestor of CRISPR-Cas12 that offers a promising platform for genome engineering. However, the genome-editing activity of TnpBs remains limit...
www.biorxiv.org
January 10, 2026 at 5:16 PM
New preprint 👉Doudna x Bryant x Jacobsen x Savage collaboration!
Work led by @zehanzhou.bsky.social, I. Saffarian-Deemyad, @honglue.bsky.social, T. Weiss
We dissect how stepwise DNA unwinding gates TnpB genome editing, revealing how unwound DNA states enhance cleavage
www.biorxiv.org/content/10.6...
Work led by @zehanzhou.bsky.social, I. Saffarian-Deemyad, @honglue.bsky.social, T. Weiss
We dissect how stepwise DNA unwinding gates TnpB genome editing, revealing how unwound DNA states enhance cleavage
www.biorxiv.org/content/10.6...
Excited to share: DNA glycosylases are diverse antiviral effectors. They recognize phage base modifications and initiate genome destruction. A structure‑guided approach made the scope of this discovery possible! 🧪 #phagesky doi.org/10.1101/2025... #phage #microbiology
Antiviral Defence is a Conserved Function of Diverse DNA Glycosylases
Bacteria are frequently attacked by viruses, known as phages, and rely on diverse defence systems like restriction endonucleases and CRISPR-Cas to survive. While phages can evade these defences by cov...
doi.org
October 30, 2025 at 12:16 PM
Excited to share: DNA glycosylases are diverse antiviral effectors. They recognize phage base modifications and initiate genome destruction. A structure‑guided approach made the scope of this discovery possible! 🧪 #phagesky doi.org/10.1101/2025... #phage #microbiology
Just amazing what evolution can come up with. Naturally occurring nuclease deficient Cas12 family members use gRNAs to bind DNA and recruit sigmaE family members that drive txn initiation proximally. 2 papers! 1- describes 2- structure!
www.nature.com/articles/s41... www.nature.com/articles/s41...
www.nature.com/articles/s41... www.nature.com/articles/s41...
Exapted CRISPR–Cas12f homologues drive RNA-guided transcription - Nature
Specialized σ factors interact with nuclease-dead, CRISPR–Cas12f proteins to form potent, RNA-guided gene activation systems that function independently of fixed promoter motifs.
www.nature.com
March 4, 2026 at 5:06 PM
Just amazing what evolution can come up with. Naturally occurring nuclease deficient Cas12 family members use gRNAs to bind DNA and recruit sigmaE family members that drive txn initiation proximally. 2 papers! 1- describes 2- structure!
www.nature.com/articles/s41... www.nature.com/articles/s41...
www.nature.com/articles/s41... www.nature.com/articles/s41...
VIPR RNA-guided DNA recognition by noncontiguous geometric triplex formation | Science www.science.org/doi/10.1126/...
VIPR RNA-guided DNA recognition by noncontiguous geometric triplex formation
Viral interference programmable repeat (VIPR) systems use a noncontiguous code for RNA-guided transcriptional silencing. How the Vipr protein and a VIPR RNA (vrRNA) comprising alternating GGY and NN s...
www.science.org
September 18, 2026 at 1:14 AM
VIPR RNA-guided DNA recognition by noncontiguous geometric triplex formation | Science www.science.org/doi/10.1126/...
すごいじゃない、なんか笑っちゃうくらい神の怒りを誘って大災害起きそうなこと早速やってんじゃない
この世に“存在しない”DNA配列をAIが創造。生成した人工DNA配列をマウスや魚に組み込む（生成AIクローズアップ） www.techno-edge.net/article/2024...
この世に“存在しない”DNA配列をAIが創造。生成した人工DNA配列をマウスや魚に組み込む（生成AIクローズアップ） www.techno-edge.net/article/2024...
この世に“存在しない”DNA配列をAIが創造。生成した人工DNA配列をマウスや魚に組み込む（生成AIクローズアップ） | テクノエッジ TechnoEdge
今回は、自然界に存在しないDNA配列を生成できるAIシステムを提案した論文「Machine-guided design of cell-type-targeting cis-regulatory elements」に注目します。
www.techno-edge.net
November 7, 2024 at 2:39 PM
すごいじゃない、なんか笑っちゃうくらい神の怒りを誘って大災害起きそうなこと早速やってんじゃない
この世に“存在しない”DNA配列をAIが創造。生成した人工DNA配列をマウスや魚に組み込む（生成AIクローズアップ） www.techno-edge.net/article/2024...
この世に“存在しない”DNA配列をAIが創造。生成した人工DNA配列をマウスや魚に組み込む（生成AIクローズアップ） www.techno-edge.net/article/2024...