#fluorescencefriday Astrocytes double labeled for GFAP and HM3Dq-DREADD. Courtesy of Alycia Crooks PhD students in the lab. 🧪
June 6, 2025 at 2:35 PM
#fluorescencefriday Astrocytes double labeled for GFAP and HM3Dq-DREADD. Courtesy of Alycia Crooks PhD students in the lab. 🧪
7/11 Chemogenetically silencing a12 (hM4Di DREADD + DCZ), or dampening a12 terminals in LHA, raised heart rate.
September 25, 2026 at 2:16 AM
7/11 Chemogenetically silencing a12 (hM4Di DREADD + DCZ), or dampening a12 terminals in LHA, raised heart rate.
#fluorescencefriday brain section of a transgenic line expressing DREADD in astrocytes and the zoomed image
November 15, 2024 at 2:21 PM
#fluorescencefriday brain section of a transgenic line expressing DREADD in astrocytes and the zoomed image
fave weapons in each class
vH-3 vbigswig vpencil
sblast92 vinkbrush
vballpoint dreadd
cdousers ucb
dflux vstamper
vH-3 vbigswig vpencil
sblast92 vinkbrush
vballpoint dreadd
cdousers ucb
dflux vstamper
@mello450.bsky.social ‘s weapons of each class he would dread playing the least (ordered)
vflux
vimi
vsquiff
l3d
ntri
vink
cdousers
sbrella
drapid
vroller
vstamper
vflux
vimi
vsquiff
l3d
ntri
vink
cdousers
sbrella
drapid
vroller
vstamper
woah!!!!! fav weapons of each class time
- vSploosh
- vSwig
- cGoo
- Dread
- vMini
- nDapple
- vUnder
- nPainbrush
- do i need to answer this one (vLuna)
- vWellstring
- vWiper
i dont like hammer but i like hammer
- vSploosh
- vSwig
- cGoo
- Dread
- vMini
- nDapple
- vUnder
- nPainbrush
- do i need to answer this one (vLuna)
- vWellstring
- vWiper
i dont like hammer but i like hammer
October 18, 2024 at 2:16 PM
fave weapons in each class
vH-3 vbigswig vpencil
sblast92 vinkbrush
vballpoint dreadd
cdousers ucb
dflux vstamper
vH-3 vbigswig vpencil
sblast92 vinkbrush
vballpoint dreadd
cdousers ucb
dflux vstamper
A DREADD for the periphery - Nature Methods
Nature Methods - A DREADD for the periphery
www.nature.com
February 11, 2025 at 8:57 PM
Longitudinal assessment of DREADD expression and efficacy in the monkey brain https://www.biorxiv.org/content/10.1101/2024.12.26.630299v1
December 26, 2024 at 1:15 PM
Longitudinal assessment of DREADD expression and efficacy in the monkey brain https://www.biorxiv.org/content/10.1101/2024.12.26.630299v1
June 30, 2026 at 9:00 PM
WOW!!
A humanized Gs-coupled DREADD for circuit and behavior modulation
www.frontiersin.org/journals/cel...
A humanized Gs-coupled DREADD for circuit and behavior modulation
www.frontiersin.org/journals/cel...
Frontiers | A humanized Gs-coupled DREADD for circuit and behavior modulation
www.frontiersin.org
April 20, 2025 at 8:40 PM
WOW!!
A humanized Gs-coupled DREADD for circuit and behavior modulation
www.frontiersin.org/journals/cel...
A humanized Gs-coupled DREADD for circuit and behavior modulation
www.frontiersin.org/journals/cel...
Completely agree! E.g: In this preprint, I co-expressed axon-GCaMP and inhibitory DREADD (H4) and showed that local CNO infusion attenuates terminal Ca2+ during singing. In my case, I wanted to validate the DREADD, but this can also be used the other way around (1/3)
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
January 28, 2026 at 5:21 PM
Completely agree! E.g: In this preprint, I co-expressed axon-GCaMP and inhibitory DREADD (H4) and showed that local CNO infusion attenuates terminal Ca2+ during singing. In my case, I wanted to validate the DREADD, but this can also be used the other way around (1/3)
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
As we say start to say goodbye to 2024, let's not say goodbye to our DREADD-free control groups. HUGS!
December 30, 2024 at 3:51 AM
As we say start to say goodbye to 2024, let's not say goodbye to our DREADD-free control groups. HUGS!
Now I'm envisioning a satire horror skit where someone keeps writing these scary sounding messages that are just science references
"Which ones will we sac next?"
"Be prepared to cut & run"
"Existential DREADD"
"Which ones will we sac next?"
"Be prepared to cut & run"
"Existential DREADD"
January 16, 2025 at 7:59 PM
Now I'm envisioning a satire horror skit where someone keeps writing these scary sounding messages that are just science references
"Which ones will we sac next?"
"Be prepared to cut & run"
"Existential DREADD"
"Which ones will we sac next?"
"Be prepared to cut & run"
"Existential DREADD"
Imma preface this by saying I haven't played any GOTY nominees lol
1. Without a Dawn
2. Schedule 1
3. Gone Fishing
4. Satisfactory
5. Dreadd Flats
6. RV There Yet?
#indiegamer #gameoftheyear #goty #indiegames #horrorgames
1. Without a Dawn
2. Schedule 1
3. Gone Fishing
4. Satisfactory
5. Dreadd Flats
6. RV There Yet?
#indiegamer #gameoftheyear #goty #indiegames #horrorgames
November 20, 2025 at 12:08 PM
Imma preface this by saying I haven't played any GOTY nominees lol
1. Without a Dawn
2. Schedule 1
3. Gone Fishing
4. Satisfactory
5. Dreadd Flats
6. RV There Yet?
#indiegamer #gameoftheyear #goty #indiegames #horrorgames
1. Without a Dawn
2. Schedule 1
3. Gone Fishing
4. Satisfactory
5. Dreadd Flats
6. RV There Yet?
#indiegamer #gameoftheyear #goty #indiegames #horrorgames
Constitutive DREADD signalling modulates oligodendrocyte precursor cell bioelectrical membrane properties and fate https://www.biorxiv.org/content/10.64898/2025.12.22.695316v1
December 24, 2025 at 9:15 PM
Constitutive DREADD signalling modulates oligodendrocyte precursor cell bioelectrical membrane properties and fate https://www.biorxiv.org/content/10.64898/2025.12.22.695316v1
I am glad to share our recent work, now published in Cell! A @zenbrainest.bsky.social and Scherrer lab collaboration developing and validating a new peripherally restricted DREADD system! Great team work with Hye Jin Kang, Brian Krumm, @adrtas.bsky.social, and others
www.cell.com/cell/abstrac...
www.cell.com/cell/abstrac...
Structure-guided design of a peripherally restricted chemogenetic system
Kang et al. present HCAD, a peripherally restricted DREADD based on HCA2, with a chemical actuator that avoids crossing the blood-brain barrier and selectively reduces pain in mice. This system will enable the study of peripheral physiology without affecting the central nervous system.
www.cell.com
December 7, 2024 at 1:41 AM
I am glad to share our recent work, now published in Cell! A @zenbrainest.bsky.social and Scherrer lab collaboration developing and validating a new peripherally restricted DREADD system! Great team work with Hye Jin Kang, Brian Krumm, @adrtas.bsky.social, and others
www.cell.com/cell/abstrac...
www.cell.com/cell/abstrac...
@sfn.org Come say hi to postdoc Blake Kimmey Tues, Nov. 18, 8:00 AM - 12:00 PM
PSTR278.07 - Convergent state-control of endogenous opioid analgesia
He's got new behavior/DREADD/Xenium data on PAG-to-thalamic circuits in pain/placebo/fear, building from his preprint: biorxiv.org/content/10.1...
PSTR278.07 - Convergent state-control of endogenous opioid analgesia
He's got new behavior/DREADD/Xenium data on PAG-to-thalamic circuits in pain/placebo/fear, building from his preprint: biorxiv.org/content/10.1...
November 17, 2025 at 5:47 PM
@sfn.org Come say hi to postdoc Blake Kimmey Tues, Nov. 18, 8:00 AM - 12:00 PM
PSTR278.07 - Convergent state-control of endogenous opioid analgesia
He's got new behavior/DREADD/Xenium data on PAG-to-thalamic circuits in pain/placebo/fear, building from his preprint: biorxiv.org/content/10.1...
PSTR278.07 - Convergent state-control of endogenous opioid analgesia
He's got new behavior/DREADD/Xenium data on PAG-to-thalamic circuits in pain/placebo/fear, building from his preprint: biorxiv.org/content/10.1...
"Dr. Brandon & Dr. Bradley Brooks introduce DREADD (Diabetes-Related Extremity Amputation Depression & Distress), highlighting the emotional toll of amputations and calling for mental health integration in limb care. 🧠🩺
#MedSky #Podiatry
news.va.gov/136897/colum...
#MedSky #Podiatry
news.va.gov/136897/colum...
Columbia VA podiatrist recognized for diabetes-related amputation research - VA News
The abstract DREADD describes the emotional and psychological toll experienced by patients following any diabetes-related amputation.
news.va.gov
December 20, 2024 at 2:15 PM
"Dr. Brandon & Dr. Bradley Brooks introduce DREADD (Diabetes-Related Extremity Amputation Depression & Distress), highlighting the emotional toll of amputations and calling for mental health integration in limb care. 🧠🩺
#MedSky #Podiatry
news.va.gov/136897/colum...
#MedSky #Podiatry
news.va.gov/136897/colum...
Vzap
Vcarbon
Fsquiffer
DreadD
Bpn
Vglooga/Vtetra
Vtent
Cblaster/Vsblast
Vinkbrush
Inkline
CHARCOAL
Vcarbon
Fsquiffer
DreadD
Bpn
Vglooga/Vtetra
Vtent
Cblaster/Vsblast
Vinkbrush
Inkline
CHARCOAL
52deco
Carbon deco
FSquiffer
Slosher
Gold naut
Glooga deco
Sorella undercover
RANGE BLASTER
Pain brush
Wellstring
Stamper
Carbon deco
FSquiffer
Slosher
Gold naut
Glooga deco
Sorella undercover
RANGE BLASTER
Pain brush
Wellstring
Stamper
first trend of bluesky yippeee
favourite weapon of each class
- nzap
- carbon deco
- pencil
- cexplo
- bp
- light tetra
- sorella brella
- rapid pro deco
- octobrush
- vlux
- stamper
favourite weapon of each class
- nzap
- carbon deco
- pencil
- cexplo
- bp
- light tetra
- sorella brella
- rapid pro deco
- octobrush
- vlux
- stamper
October 17, 2024 at 9:36 PM
Vzap
Vcarbon
Fsquiffer
DreadD
Bpn
Vglooga/Vtetra
Vtent
Cblaster/Vsblast
Vinkbrush
Inkline
CHARCOAL
Vcarbon
Fsquiffer
DreadD
Bpn
Vglooga/Vtetra
Vtent
Cblaster/Vsblast
Vinkbrush
Inkline
CHARCOAL
Off-target effects of DREADD ligands revealed by an anesthesia emergence paradigm in mice
Off-target effects of DREADD ligands revealed by an anesthesia emergence paradigm in mice
Moreno-Gomez et al. demonstrate that CNO, C21, and DCZ delay anesthesia emergence in mice. They show that subcutaneous administration mitigates CNO effects and provide an open-source deep learning pipeline to rigorously screen chemogenetic tools. Overall, they demonstrate that rigorous chemogenetic research requires mitigating off-target actions via careful dosing and DREADD-free controls.
dlvr.it
July 17, 2026 at 10:59 PM
Off-target effects of DREADD ligands revealed by an anesthesia emergence paradigm in mice
Our new peripheral DREADD plasmid is now available via ADDGENE:
www.addgene.org/228902/
Paper here: www.addgene.org/browse/artic...
www.addgene.org/228902/
Paper here: www.addgene.org/browse/artic...
Addgene: pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine
Plasmid pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine from Dr. Bryan Roth's lab contains the insert hydroxycarboxylic acid receptor 2 and is published in Cell, 2024 This plasmid is available through Addgene.
www.addgene.org
December 4, 2024 at 9:26 PM
Our new peripheral DREADD plasmid is now available via ADDGENE:
www.addgene.org/228902/
Paper here: www.addgene.org/browse/artic...
www.addgene.org/228902/
Paper here: www.addgene.org/browse/artic...
dreadd stared at it...dumbfounded and frightened as he could still hear and feel his heartbeat...
"...that...cannot be normal..."
"...that...cannot be normal..."
April 3, 2026 at 7:47 AM
dreadd stared at it...dumbfounded and frightened as he could still hear and feel his heartbeat...
"...that...cannot be normal..."
"...that...cannot be normal..."
Naturally we were blown away by this and decided to follow up onit. Aaron went on to show that DREADD inhibition of PrL CST+ neurons results in increased kindling rate ~exclusively~ in female mice. Another surprising finding.
February 2, 2026 at 5:22 PM
Naturally we were blown away by this and decided to follow up onit. Aaron went on to show that DREADD inhibition of PrL CST+ neurons results in increased kindling rate ~exclusively~ in female mice. Another surprising finding.
Eh, since when has Hollywood, especially Disney, shown such sense?
I'd say 50-50 chance they'll do like the 90's attempt at Judge Dreadd and have him spend a lot of the movie without the helmet juat so the audience can see where the money went.
I'd say 50-50 chance they'll do like the 90's attempt at Judge Dreadd and have him spend a lot of the movie without the helmet juat so the audience can see where the money went.
August 15, 2026 at 6:38 AM
Eh, since when has Hollywood, especially Disney, shown such sense?
I'd say 50-50 chance they'll do like the 90's attempt at Judge Dreadd and have him spend a lot of the movie without the helmet juat so the audience can see where the money went.
I'd say 50-50 chance they'll do like the 90's attempt at Judge Dreadd and have him spend a lot of the movie without the helmet juat so the audience can see where the money went.
Just under the 2024 wire! Qiaowei Xie's final thesis paper showed that DREADD activation of mPFC-NAc shell projections could suppress elevated CPP reinstatement in the EcoHIV model, suggesting common circuit contribution to behavior in a HIV mouse model.
Chemogenetic Activation of Medial Prefrontal Cortex Projections to the Nucleus Accumbens Shell Suppresses Cocaine-Primed Reinstatement in EcoHIV Infected Mice https://www.biorxiv.org/content/10.1101/2024.12.20.629781v1
December 22, 2024 at 2:27 PM
Just under the 2024 wire! Qiaowei Xie's final thesis paper showed that DREADD activation of mPFC-NAc shell projections could suppress elevated CPP reinstatement in the EcoHIV model, suggesting common circuit contribution to behavior in a HIV mouse model.