Story about EMCV IRES interactions with the ribosome:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
June 12, 2025 at 10:05 PM
Story about EMCV IRES interactions with the ribosome:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Heureusement, Révolution Permanente voit clair dans leur jeu et propose une alternative avec la lutte des classes et la candidature d'Anasse Kazib.
www.youtube.com/watch?v=EMCv...
www.youtube.com/watch?v=EMCv...
Mélenchon DÉFEND l'IMPÉRIALISME FRANÇAIS sur LCI ?? On REACT à son interview - INTERREGNE
YouTube video by Révolution Permanente
www.youtube.com
October 5, 2026 at 11:39 PM
Heureusement, Révolution Permanente voit clair dans leur jeu et propose une alternative avec la lutte des classes et la candidature d'Anasse Kazib.
www.youtube.com/watch?v=EMCv...
www.youtube.com/watch?v=EMCv...
But polio? No. My colleague Juliet Morrison was making EMCV-polio chimeras and made an entire library of them in a third of the time it took me to clone RV1A. At first she said it’s because she’s better at cloning than me (she is) but then I said let’s see if you can do better.
December 18, 2024 at 1:07 PM
But polio? No. My colleague Juliet Morrison was making EMCV-polio chimeras and made an entire library of them in a third of the time it took me to clone RV1A. At first she said it’s because she’s better at cloning than me (she is) but then I said let’s see if you can do better.
New paper: collaboration with Cambridge on EMCV 2B* now published journals.asm.org/doi/10.1128/...
Encephalomyocarditis virus protein 2B* interacts with 14-3-3 proteins through a phosphorylated C-terminal binding motif | mBio
Encephalomyocarditis virus (EMCV) infects a range of species, causing economically
important reproductive disorders in pigs and encephalitis and myocarditis in rodents.
Due to its wide host range, it ...
journals.asm.org
August 23, 2025 at 9:02 AM
New paper: collaboration with Cambridge on EMCV 2B* now published journals.asm.org/doi/10.1128/...
Cryo-EM studies of 48S initiation complexes formed on the EMCV IRES reveal specific contacts that are functionally required for 5’end independent translation initiation
Tatyana Pestova, Christopher Hellen, @joachim123.bsky.social and colleagues
link.springer.com/article/10.1...
Tatyana Pestova, Christopher Hellen, @joachim123.bsky.social and colleagues
link.springer.com/article/10.1...
The mechanism of ribosomal recruitment during translation initiation on the Type 2 encephalomyocarditis virus IRES - The EMBO Journal
The encephalomyocarditis virus (EMCV) internal ribosomal entry side (IRES) and other Type 2 IRESs favor translation of the viral genome during infection. The domains H-L of these IRESs specifically in...
link.springer.com
March 18, 2026 at 3:59 PM
Cryo-EM studies of 48S initiation complexes formed on the EMCV IRES reveal specific contacts that are functionally required for 5’end independent translation initiation
Tatyana Pestova, Christopher Hellen, @joachim123.bsky.social and colleagues
link.springer.com/article/10.1...
Tatyana Pestova, Christopher Hellen, @joachim123.bsky.social and colleagues
link.springer.com/article/10.1...
2/10
MDA5 binds long dsRNA and is activated during viral infections like #SARS-CoV-2 and EMCV.
Using iCLIP to sequence MDA5-bound RNA during infection, we found it overwhelmingly bound human RNA. This RNA was mostly intronic and near repetitive elements like Alus prone to forming dsRNA.
MDA5 binds long dsRNA and is activated during viral infections like #SARS-CoV-2 and EMCV.
Using iCLIP to sequence MDA5-bound RNA during infection, we found it overwhelmingly bound human RNA. This RNA was mostly intronic and near repetitive elements like Alus prone to forming dsRNA.
August 11, 2026 at 11:10 AM
2/10
MDA5 binds long dsRNA and is activated during viral infections like #SARS-CoV-2 and EMCV.
Using iCLIP to sequence MDA5-bound RNA during infection, we found it overwhelmingly bound human RNA. This RNA was mostly intronic and near repetitive elements like Alus prone to forming dsRNA.
MDA5 binds long dsRNA and is activated during viral infections like #SARS-CoV-2 and EMCV.
Using iCLIP to sequence MDA5-bound RNA during infection, we found it overwhelmingly bound human RNA. This RNA was mostly intronic and near repetitive elements like Alus prone to forming dsRNA.
These findings are similar to recent work on the EMCV Type 2 IRES — see e.g. www.biorxiv.org/content/10.1...; www.biorxiv.org/content/10.1... — and suggest that common structural motifs and mechanisms may exist within highly divergent picornavirus IRES sequences
The mechanism of ribosomal recruitment during translation initiation on Type 2 IRESs
The encephalomyocarditis virus (EMCV) IRES and other Type 2 IRESs comprise domains H-L and specifically interact with eIF4G/eIF4A through their essential JK domain. However, the JK domain is not suffi...
www.biorxiv.org
October 7, 2025 at 11:37 AM
These findings are similar to recent work on the EMCV Type 2 IRES — see e.g. www.biorxiv.org/content/10.1...; www.biorxiv.org/content/10.1... — and suggest that common structural motifs and mechanisms may exist within highly divergent picornavirus IRES sequences
📍Atenció! Estem buscant algú que es vulgui unir al nostre equip de Cohesió Social i Urbana a @institutmetropoli.cat. Treballarem temes relacionats amb la desigualtat social i la pobresa des d'una perspectiva urbana. #EMCV
Tota la info aquí 👇
www.institutmetropoli.cat/noticias/ofe...
Tota la info aquí 👇
www.institutmetropoli.cat/noticias/ofe...
Busquem investigador/a per desenvolupar estudis de cohesió social
Si us interessa, us hi podeu presentar fins al pròxim 4 de març de 2025
www.institutmetropoli.cat
February 20, 2025 at 8:27 AM
📍Atenció! Estem buscant algú que es vulgui unir al nostre equip de Cohesió Social i Urbana a @institutmetropoli.cat. Treballarem temes relacionats amb la desigualtat social i la pobresa des d'una perspectiva urbana. #EMCV
Tota la info aquí 👇
www.institutmetropoli.cat/noticias/ofe...
Tota la info aquí 👇
www.institutmetropoli.cat/noticias/ofe...
For only 2/3rds of what the public is obligated to stump up to pay EMcV so she can tweet this crap all day. Bargain.
November 2, 2025 at 10:42 PM
For only 2/3rds of what the public is obligated to stump up to pay EMcV so she can tweet this crap all day. Bargain.
Many RNA viruses use programmed -1 ribosomal frameshifting as an essential gene expression strategy. Cardioviruses (e.g. EMCV, TMEV) are the absolute masters of this, causing ~85 % of ribosomes to 'slip' into the -1 reading frame.
2/8
2/8
August 24, 2026 at 11:52 AM
Many RNA viruses use programmed -1 ribosomal frameshifting as an essential gene expression strategy. Cardioviruses (e.g. EMCV, TMEV) are the absolute masters of this, causing ~85 % of ribosomes to 'slip' into the -1 reading frame.
2/8
2/8
Critically, when ANXA2 is absent, cells fail to induce strong innate immune responses to virus infections such as #westnilevirus #EMCV and #influenzaA. Amazingly, cells hardly "realize" they are infected with West Nile Virus if ANXA2 is absent!
November 17, 2025 at 6:21 AM
Critically, when ANXA2 is absent, cells fail to induce strong innate immune responses to virus infections such as #westnilevirus #EMCV and #influenzaA. Amazingly, cells hardly "realize" they are infected with West Nile Virus if ANXA2 is absent!
3/9
Since MDA5 operates in the cytosol, we looked at cytosolic RNA during infection.
Total RNA-seq showed both SARS and EMCV trigger a surge of aberrant cytosolic RNA, including intronic & transcribed upstream, downstream, or opposite annotated genes. Basically, weird RNA that shouldn't be there!
Since MDA5 operates in the cytosol, we looked at cytosolic RNA during infection.
Total RNA-seq showed both SARS and EMCV trigger a surge of aberrant cytosolic RNA, including intronic & transcribed upstream, downstream, or opposite annotated genes. Basically, weird RNA that shouldn't be there!
Keegan Michael Key: What For Real Reaction
ALT: Keegan Michael Key: What For Real Reaction
static.klipy.com
August 11, 2026 at 11:10 AM
3/9
Since MDA5 operates in the cytosol, we looked at cytosolic RNA during infection.
Total RNA-seq showed both SARS and EMCV trigger a surge of aberrant cytosolic RNA, including intronic & transcribed upstream, downstream, or opposite annotated genes. Basically, weird RNA that shouldn't be there!
Since MDA5 operates in the cytosol, we looked at cytosolic RNA during infection.
Total RNA-seq showed both SARS and EMCV trigger a surge of aberrant cytosolic RNA, including intronic & transcribed upstream, downstream, or opposite annotated genes. Basically, weird RNA that shouldn't be there!
get your grind on...
www.youtube.com/watch?v=emcv...
www.youtube.com/watch?v=emcv...
Killing Joke - Total Invasion (HD)
YouTube video by pigsfood27
www.youtube.com
February 14, 2025 at 4:36 PM
get your grind on...
www.youtube.com/watch?v=emcv...
www.youtube.com/watch?v=emcv...
🟡NOVO VÍDEO NO RANDOMRICKY🟡
Neste vídeo eu e os meus amigos jogamos Risk e tornamo-nos Ditadores do mundo >:D
Editado pela fantástica PokeorRC e Co-Editado por mim
Vão ver, ficou um vídeo fixolas :D
(www.youtube.com/watch?v=eMCV...)
Neste vídeo eu e os meus amigos jogamos Risk e tornamo-nos Ditadores do mundo >:D
Editado pela fantástica PokeorRC e Co-Editado por mim
Vão ver, ficou um vídeo fixolas :D
(www.youtube.com/watch?v=eMCV...)
November 27, 2024 at 1:06 PM
🟡NOVO VÍDEO NO RANDOMRICKY🟡
Neste vídeo eu e os meus amigos jogamos Risk e tornamo-nos Ditadores do mundo >:D
Editado pela fantástica PokeorRC e Co-Editado por mim
Vão ver, ficou um vídeo fixolas :D
(www.youtube.com/watch?v=eMCV...)
Neste vídeo eu e os meus amigos jogamos Risk e tornamo-nos Ditadores do mundo >:D
Editado pela fantástica PokeorRC e Co-Editado por mim
Vão ver, ficou um vídeo fixolas :D
(www.youtube.com/watch?v=eMCV...)
This is a hugely impressive and long-anticipated structural analysis of the ribosomal 48S pre-initiation complex formed with the EMCV IRES. Just a shame that PTB1, one of my favourite proteins(!), is not visible in the structure.
elifesciences.org/articles/107...
elifesciences.org/articles/107...
Structural insights into the recruitment of viral type 2 IRES to ribosomal preinitiation complex for protein synthesis
The viral type 2 internal ribosome entry site uses a unique mechanism to hijack the host ribosomal machinery by interacting with initiator tRNA and 40S head.
elifesciences.org
June 29, 2026 at 4:13 PM
This is a hugely impressive and long-anticipated structural analysis of the ribosomal 48S pre-initiation complex formed with the EMCV IRES. Just a shame that PTB1, one of my favourite proteins(!), is not visible in the structure.
elifesciences.org/articles/107...
elifesciences.org/articles/107...
Many RNA viruses use programmed -1 ribosomal frameshifting as an essential gene expression strategy. Cardioviruses (e.g. EMCV, TMEV) are the absolute masters of this, causing ~85 % of ribosomes to 'slip' into the -1 reading frame.
July 19, 2025 at 3:10 PM
Many RNA viruses use programmed -1 ribosomal frameshifting as an essential gene expression strategy. Cardioviruses (e.g. EMCV, TMEV) are the absolute masters of this, causing ~85 % of ribosomes to 'slip' into the -1 reading frame.
« Il y a une page à écrire à La Rochelle, c’est ça qui m’habite » : Nolann Le Garrec s’exprime sur son adaptation au Stade Rochelais, à son public, et sur l’ambition qui l’anime
🔗https://l.sudouest.fr/EmCv
🔗https://l.sudouest.fr/EmCv
August 26, 2026 at 10:32 AM
« Il y a une page à écrire à La Rochelle, c’est ça qui m’habite » : Nolann Le Garrec s’exprime sur son adaptation au Stade Rochelais, à son public, et sur l’ambition qui l’anime
🔗https://l.sudouest.fr/EmCv
🔗https://l.sudouest.fr/EmCv
Researchers in China found that EMCV enters cells by triggering a cascade involving ICAM-1, Src, Ezrin, and Caveolin-1.
Blocking this pathway hinders infection, revealing a new antiviral target.
journals.asm.org/doi/10.1128/...
Blocking this pathway hinders infection, revealing a new antiviral target.
journals.asm.org/doi/10.1128/...
ICAM-1-mediated Src signaling pathway plays a pivotal role in encephalomyocarditis virus entry | Journal of Virology
EMCV has a wide host range and is a potential zoonotic pathogen, yet the mechanism of its entry into cells remains unclear. This study has found that the host proteins Src, Fyn, and Ezrin are involved in viral replication and proliferation, and their ...
journals.asm.org
July 9, 2025 at 1:55 PM
Researchers in China found that EMCV enters cells by triggering a cascade involving ICAM-1, Src, Ezrin, and Caveolin-1.
Blocking this pathway hinders infection, revealing a new antiviral target.
journals.asm.org/doi/10.1128/...
Blocking this pathway hinders infection, revealing a new antiviral target.
journals.asm.org/doi/10.1128/...
📢 NEW PREPRINT | Discover how alternative splicing at the OAS2 gene diversifies antiviral profiles
The shorter p69 isoform targets HCoV-OC43, while the longer p71 isoform restricts EMCV. Mechanistic plasticity reveals novel antiviral mechanisms.
www.biorxiv.org/content/10.1...
The shorter p69 isoform targets HCoV-OC43, while the longer p71 isoform restricts EMCV. Mechanistic plasticity reveals novel antiviral mechanisms.
www.biorxiv.org/content/10.1...
March 12, 2025 at 3:35 PM
📢 NEW PREPRINT | Discover how alternative splicing at the OAS2 gene diversifies antiviral profiles
The shorter p69 isoform targets HCoV-OC43, while the longer p71 isoform restricts EMCV. Mechanistic plasticity reveals novel antiviral mechanisms.
www.biorxiv.org/content/10.1...
The shorter p69 isoform targets HCoV-OC43, while the longer p71 isoform restricts EMCV. Mechanistic plasticity reveals novel antiviral mechanisms.
www.biorxiv.org/content/10.1...
RRID:CVCL_1915 was used by the authors of the Journal of General Virology paper "The EMCV protein 2B* is required for efficient cell lysis via both caspase-3-dependent and -independent pathways during infection". RRIDs like this improve reproducibility in scientific research.
doi.org
February 26, 2025 at 8:01 AM
RRID:CVCL_1915 was used by the authors of the Journal of General Virology paper "The EMCV protein 2B* is required for efficient cell lysis via both caspase-3-dependent and -independent pathways during infection". RRIDs like this improve reproducibility in scientific research.
ICAM-1-mediated Src signaling pathway plays a pivotal role in encephalomyocarditis virus entry journals.asm.org/doi/10.1128/...
ICAM-1-mediated Src signaling pathway plays a pivotal role in encephalomyocarditis virus entry | Journal of Virology
EMCV has a wide host range and is a potential zoonotic pathogen, yet the mechanism
of its entry into cells remains unclear. This study has found that the host proteins
Src, Fyn, and Ezrin are involved...
journals.asm.org
July 9, 2025 at 1:49 PM
ICAM-1-mediated Src signaling pathway plays a pivotal role in encephalomyocarditis virus entry journals.asm.org/doi/10.1128/...
I’m a smoker (took it up again in lockdown) and feel absolutely no threat from the idea of banning it in pub gardens. I welcome the idea that I’ll be able to have a pint unbothered by the likes of JB, NF, or EMcV.
August 29, 2024 at 8:08 PM
I’m a smoker (took it up again in lockdown) and feel absolutely no threat from the idea of banning it in pub gardens. I welcome the idea that I’ll be able to have a pint unbothered by the likes of JB, NF, or EMcV.
Loss of cellular RNA homeostasis contributes to MDA5 activation during virus infection
->Nature | More on "Viral immunity and cellular RNA responses" at BigEarthData.ai | #virus infection
->Nature | More on "Viral immunity and cellular RNA responses" at BigEarthData.ai | #virus infection
Loss of cellular RNA homeostasis contributes to MDA5 activation during virus infection
Previous studies of RNAs binding to and/or activating MDA5 relied on recombinant or overexpressed protein40,42,43. To identify RNA ligands of endogenous MDA5, we screened cell lines for expression of MDA5 and found that monocytic THP1 cells expressed MDA5 protein at baseline (Extended Data Fig. 1a). EMCV specifically activates MDA5 (refs. 7,44) and infects THP1 cells, evident from staining with the J2 monoclonal antibody that detects dsRNA (Extended Data Fig. 1b), a signature of viral replication39,45,46, and from accumulation of viral RNA (Extended Data Fig. 1c). Infection induced IFNB1 mRNA and IFN-stimulated genes (ISGs) (Extended Data Fig. 1d). Transfection of total cellular RNA extracted from EMCV-infected THP1 cells activated IFNB1 promoter-driven luciferase expression in HEK293 reporter cells47 in an MDA5-dependent manner (Extended Data Fig. 1e). Together, these data show that MDA5-stimulatory RNA was present in EMCV-infected THP1 cells. To obtain an in-depth view of RNAs binding MDA5 during viral infection, we employed iCLIP48. This technique allows deep sequencing of RNAs bound by a protein of interest, with resolution of the nucleotide site(s) where the protein binds, and has been successfully used for other dsRNA binding proteins including Dicer49 and DDX17 (ref. 50). As a control, MDA5 knockout (KO) THP1 cells were...
www.nature.com
August 12, 2026 at 5:03 AM
9/n So, Marcos tested three more viruses - TBEV ⬇️ & EMCV in collaboration with labs of Dan Ruzek, Martin Palus & and LCMV with @Ondrej Stepanek. Got basically nothing. Some evidence of processing into small RNAs, no antiviral effects. Removing innate immunity sensor PKR had no effect.
January 21, 2025 at 2:05 PM
9/n So, Marcos tested three more viruses - TBEV ⬇️ & EMCV in collaboration with labs of Dan Ruzek, Martin Palus & and LCMV with @Ondrej Stepanek. Got basically nothing. Some evidence of processing into small RNAs, no antiviral effects. Removing innate immunity sensor PKR had no effect.