I am happy to share our new article on dynamic Eps15 and Dab2 networks in endocytosis studied by #NMR, and forming #LLPS. Research was done at @fmp-berlin.de and at the Institut de Biologie Structurale (IBS).
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Promiscuous and multivalent interactions between Eps15 and partner protein Dab2 generate a complex interaction network - Nature Communications
Eps15, a protein involved in endocytosis initiation, uses small folded EH domains to bind disordered partner proteins and its own disordered tail, thus forming a dynamic network via phase separation t...
www.nature.com
August 21, 2025 at 7:53 AM
I am happy to share our new article on dynamic Eps15 and Dab2 networks in endocytosis studied by #NMR, and forming #LLPS. Research was done at @fmp-berlin.de and at the Institut de Biologie Structurale (IBS).
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Happy to report the publication of our study on the mechanism of endocytic site initiation in Plos Biology: journals.plos.org/plosbiology/...
The conserved protein adaptors CALM/AP180 and FCHo1/2 cooperatively recruit Eps15 to promote the initiation of clathrin-mediated endocytosis in yeast
Clathrin-mediated endocytosis (CME) is one of the main trafficking processes, but the mechanism that initiates CME sites remain unclear. These authors show that yeast CALM/AP180 and FCHo1/2 adaptor pr...
journals.plos.org
November 20, 2024 at 5:03 AM
Happy to report the publication of our study on the mechanism of endocytic site initiation in Plos Biology: journals.plos.org/plosbiology/...
A preprint using: Eps15-pmCherryN1 (RRID:Addgene_27696) from #Addgene was published.
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #reproducibility #OpenScience
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #reproducibility #OpenScience
Promiscuous and multivalent interactions between Eps15 and partner protein Dab2 generate a complex interaction network
www.biorxiv.org
February 27, 2025 at 1:01 PM
A preprint using: Eps15-pmCherryN1 (RRID:Addgene_27696) from #Addgene was published.
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #reproducibility #OpenScience
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #reproducibility #OpenScience
Tava vendo solto ,pq o Biel tava assistindo
Eps01 - Kkkk...ô a zuera.
Eps15 - Caray...saporra é pesadinha ,hein...
Eps01 - Kkkk...ô a zuera.
Eps15 - Caray...saporra é pesadinha ,hein...
September 7, 2024 at 12:37 PM
Tava vendo solto ,pq o Biel tava assistindo
Eps01 - Kkkk...ô a zuera.
Eps15 - Caray...saporra é pesadinha ,hein...
Eps01 - Kkkk...ô a zuera.
Eps15 - Caray...saporra é pesadinha ,hein...
RRIDs were included in this None paper. Thanks for making your methods matter! #methodsmatter #ReproducibleResearch #OpenScience
Promiscuous and multivalent interactions between Eps15 and partner protein Dab2 generate a complex interaction network
doi.org
August 28, 2025 at 7:00 AM
RRIDs were included in this None paper. Thanks for making your methods matter! #methodsmatter #ReproducibleResearch #OpenScience
Ohhh good advice then! Though maybe if I start Echo after watching ep14 I'll get to the stuff in eps15&16 after I've watched it? Because it'll take longer to read
start.book
November 21, 2024 at 5:08 PM
Ohhh good advice then! Though maybe if I start Echo after watching ep14 I'll get to the stuff in eps15&16 after I've watched it? Because it'll take longer to read
A preprint using: Eps15-pmCherryN1 (RRID:Addgene_27696) from #Addgene was published.
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #STMpublishing #RRID
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #STMpublishing #RRID
Epsin1 enforces a condensation-dependent checkpoint for ubiquitylated cargo during clathrin-mediated endocytosis
www.biorxiv.org
February 20, 2025 at 1:00 PM
A preprint using: Eps15-pmCherryN1 (RRID:Addgene_27696) from #Addgene was published.
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #STMpublishing #RRID
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #STMpublishing #RRID
Like [SPOILERS] I love shamimi i really do but i was so prepared fpr the beast story to be done and all that and maybe they get redemption but it feels like they are REALLYYY dragging out shadow milk as a villian, it just feels really unsatisfying after eps15 ,, idk it just, didnt feel good
March 11, 2026 at 10:00 AM
Like [SPOILERS] I love shamimi i really do but i was so prepared fpr the beast story to be done and all that and maybe they get redemption but it feels like they are REALLYYY dragging out shadow milk as a villian, it just feels really unsatisfying after eps15 ,, idk it just, didnt feel good
Principal Investigator Jeanne Stachowiak and researchers at @utaustin.bsky.social demonstrate that protein condensates provide a platform for recruitment and assembly of clathrin triskelia using in vitro reconstitution and nuclear magnetic resonance spectroscopy. www.nature.com/articles/s41...
Of condensates and coats - reciprocal regulation of clathrin assembly and the growth of protein networks - Nature Communications
Biomolecular condensates of Eps15 recruit and assemble clathrin. In turn, clathrin assembly restricts condensate growth while imposing the preferred curvature of the clathrin lattice.
www.nature.com
November 19, 2025 at 4:54 PM
Principal Investigator Jeanne Stachowiak and researchers at @utaustin.bsky.social demonstrate that protein condensates provide a platform for recruitment and assembly of clathrin triskelia using in vitro reconstitution and nuclear magnetic resonance spectroscopy. www.nature.com/articles/s41...
EPS15L1:p (epidermal growth factor receptor pathway substrate 15 like 1) is an evolutionarily conserved paralog of EPS15:p. It has a similar arrangement of the 4 nward structured domains, but has detailed differences wrt its phosphoIDRs. The paralogs do not share any tryptic peptides. #proteomics
September 29, 2025 at 11:31 AM
EPS15L1:p (epidermal growth factor receptor pathway substrate 15 like 1) is an evolutionarily conserved paralog of EPS15:p. It has a similar arrangement of the 4 nward structured domains, but has detailed differences wrt its phosphoIDRs. The paralogs do not share any tryptic peptides. #proteomics
EPS15:p (epidermal growth factor receptor pathway substrate 15) has a single EGFR kinase signal acceptor (hs: Y849; mm: Y850), but plenty of S/T acceptors for other kinases. These acceptors flank the nward EF-hand pair & in at least 3 cward phosphoIDRs. #proteomics
September 28, 2025 at 12:11 PM
EPS15:p (epidermal growth factor receptor pathway substrate 15) has a single EGFR kinase signal acceptor (hs: Y849; mm: Y850), but plenty of S/T acceptors for other kinases. These acceptors flank the nward EF-hand pair & in at least 3 cward phosphoIDRs. #proteomics
Epidermal growth factor receptor pathway substrate 15 (EPS15:p) is named for its role as an acceptor for a Y-kinase, which does not seem to happen in practice. Instead it has multiple pS hot zones. They are simple (adjacent to the nward eponymous EH domains) or compound (in the cward pIDR).
April 17, 2025 at 12:05 PM
Epidermal growth factor receptor pathway substrate 15 (EPS15:p) is named for its role as an acceptor for a Y-kinase, which does not seem to happen in practice. Instead it has multiple pS hot zones. They are simple (adjacent to the nward eponymous EH domains) or compound (in the cward pIDR).
Feed: "Nature Communications"
By: Sigrid Milles
By: Sigrid Milles
Promiscuous and multivalent interactions between Eps15 and partner protein Dab2 generate a complex interaction network
Eps15, a protein involved in endocytosis initiation, uses small folded EH domains to bind disordered partner proteins and its own disordered tail, thus forming a dynamic network via phase separation that may help organize early endocytic events.
www.nature.com
August 21, 2025 at 5:56 PM
Feed: "Nature Communications"
By: Sigrid Milles
By: Sigrid Milles
February 13, 2026 at 3:18 PM
February 13, 2026 at 3:04 PM
Lucifer Yellow also works for vacuoles.
(I don't have the original ref handy, but here's a paper wher I used it in budding yeast www.nature.com/articles/ncb... )
(I don't have the original ref handy, but here's a paper wher I used it in budding yeast www.nature.com/articles/ncb... )
Yeast Eps15-like endocytic protein, Pan1p, activates the Arp2/3 complex - Nature Cell Biology
Nature Cell Biology - Yeast Eps15-like endocytic protein, Pan1p, activates the Arp2/3 complex
www.nature.com
March 7, 2025 at 2:40 PM
Lucifer Yellow also works for vacuoles.
(I don't have the original ref handy, but here's a paper wher I used it in budding yeast www.nature.com/articles/ncb... )
(I don't have the original ref handy, but here's a paper wher I used it in budding yeast www.nature.com/articles/ncb... )
ETF經理人明明知道鴻海今年12月開始出貨第一批GB200明年大量出貨，營收會超過8兆，EPS15元起跳，卻還是把鴻海從成分股中剃除，怎麼想都不合理
December 9, 2024 at 2:43 AM
ETF經理人明明知道鴻海今年12月開始出貨第一批GB200明年大量出貨，營收會超過8兆，EPS15元起跳，卻還是把鴻海從成分股中剃除，怎麼想都不合理
Identification of drug-like molecules targeting the ATPase activity of dynamin-like EHD4 https://www.biorxiv.org/content/10.1101/2024.04.11.589129v1
Identification of drug-like molecules targeting the ATPase activity of dynamin-like EHD4 https://www.biorxiv.org/content/10.1101/2024.04.11.589129v1
Eps15 (epidermal growth factor receptor pathway substrate 15) homology domain-containing proteins (E
www.biorxiv.org
April 12, 2024 at 3:45 AM
Identification of drug-like molecules targeting the ATPase activity of dynamin-like EHD4 https://www.biorxiv.org/content/10.1101/2024.04.11.589129v1
Competitive interactions between Eps15 EH domains and intrinsically disordered proteins generate a complex interaction network https://www.biorxiv.org/content/10.1101/2024.09.30.615448v1
Competitive interactions between Eps15 EH domains and intrinsically disordered proteins generate a complex interaction network https://www.biorxiv.org/content/10.1101/2024.09.30.615448v1
Eps15 is one of the earliest arriving proteins at the endocytic site and is of complex architecture:
www.biorxiv.org
October 1, 2024 at 8:48 PM
Competitive interactions between Eps15 EH domains and intrinsically disordered proteins generate a complex interaction network https://www.biorxiv.org/content/10.1101/2024.09.30.615448v1
