D. cerebrum13,14,15,33,35,54,60,61,62,63 were raised and maintained in conventional zebrafish housing systems (Aquaneering; system water temperature 29 ± 0.5 °C, pH 7.0, conductivity 600 µS cm−1), under 14:10 h light:dark cycles, and were fed 2–3 times a day. D. cerebrum were bred in communal tanks of ~40–60 individuals, enriched with ~5 cm silicone tubes to encourage spawning. Eggs were collected during the first 1 h of daylight, and 1–2 h after morning and afternoon feeding. Embryos (0–5 days post-fertilization (dpf)) were raised in egg-water (5 mM NaCl, 169 μM KCl, 330 μM CaCl2, 161 μM MgSO4∙7H2O, and 0.15% methylene blue dissolved in reverse osmosis purified water) in an incubator at 29 ± 0.5 °C. Larvae (5–14 dpf) were housed in static tanks in the housing systems and cocultured with L-type rotifers (Brachionus plicatilis), while water level was raised ~1 cm per day. At 21 dpf, the housing tank was connected to the water circulation system and fish were fed with artemia. At 8–10 weeks post-fertilization, D. cerebrum reach adulthood and become fertile. Wild-type D. cerebrum were provided by A. Douglass and B. Judkewitz. The transgenic line Tg(elavl3:H2B-GCaMP6s)64 was provided by B. Judkewitz. The behavioural and imaging experiments with D. cerebrum were...

The Saving Wildcats partnership is keeping an eye on the latest wildcats being bred in captivity for release into the Cairngorms.