One flowcell from @nanoporetech.com yielded 260 Gbp 🎉🚀🤯🟩
December 8, 2025 at 4:04 PM
One flowcell from @nanoporetech.com yielded 260 Gbp 🎉🚀🤯🟩
Suprise delivery on Friday afternoon from @nanoporetech.com . Will be interesting to see if the Mk1D will increase flowcell output. #nanopore
September 12, 2025 at 4:48 PM
Suprise delivery on Friday afternoon from @nanoporetech.com . Will be interesting to see if the Mk1D will increase flowcell output. #nanopore
Oxford Nanopore has updated their flowcell pricings: Minion largest discounts, some 30% cheaper than before. Flongle also cheaper. Promethion is now between $900 and $600 per flowcell.
January 7, 2024 at 9:01 AM
Oxford Nanopore has updated their flowcell pricings: Minion largest discounts, some 30% cheaper than before. Flongle also cheaper. Promethion is now between $900 and $600 per flowcell.
Blows my mind how rapidly biotech advances 🤯 on the right is a NovaSeq 6000 S4 flowcell, on the left is the new 10B NovaSeq X plus flowcell
October 26, 2023 at 2:51 PM
Blows my mind how rapidly biotech advances 🤯 on the right is a NovaSeq 6000 S4 flowcell, on the left is the new 10B NovaSeq X plus flowcell
Interested in long-read plant genomics? 🌱
We are sharing our ONT P2 Solo sequencing outputs per flowcell — explore our real data and experience here:
👉 www.izmb.uni-bonn.de/en/pbb/news#...
#Genomics #LongReads #PlantSci @puckerlab.bsky.social
We are sharing our ONT P2 Solo sequencing outputs per flowcell — explore our real data and experience here:
👉 www.izmb.uni-bonn.de/en/pbb/news#...
#Genomics #LongReads #PlantSci @puckerlab.bsky.social
November 1, 2025 at 10:47 AM
Interested in long-read plant genomics? 🌱
We are sharing our ONT P2 Solo sequencing outputs per flowcell — explore our real data and experience here:
👉 www.izmb.uni-bonn.de/en/pbb/news#...
#Genomics #LongReads #PlantSci @puckerlab.bsky.social
We are sharing our ONT P2 Solo sequencing outputs per flowcell — explore our real data and experience here:
👉 www.izmb.uni-bonn.de/en/pbb/news#...
#Genomics #LongReads #PlantSci @puckerlab.bsky.social
The preprint of the first of the batch of parasitoid wasp genomes that we are generating is now out. Erythrina gall wasp is an invasive pest on the Hawaiian wiliwili tree, we were able to generate a HiFi genome using 1/10 of a flowcell, costing only ~$100 for the sequencing.
January 26, 2026 at 1:10 AM
The preprint of the first of the batch of parasitoid wasp genomes that we are generating is now out. Erythrina gall wasp is an invasive pest on the Hawaiian wiliwili tree, we were able to generate a HiFi genome using 1/10 of a flowcell, costing only ~$100 for the sequencing.
Nanopore sequencing 96 isolates on a promethion flowcell using the rapid barcoding kit= (900+330)/96 =13 $/isolate.
Cheaper if you get discounted flowcells from bulk deals.
Cheaper if you get discounted flowcells from bulk deals.
November 20, 2024 at 6:45 PM
Nanopore sequencing 96 isolates on a promethion flowcell using the rapid barcoding kit= (900+330)/96 =13 $/isolate.
Cheaper if you get discounted flowcells from bulk deals.
Cheaper if you get discounted flowcells from bulk deals.
Also, one bad flowcell and it eats your overhead. I don’t worry about bad flowcells with outsourcing
January 20, 2026 at 9:04 PM
Also, one bad flowcell and it eats your overhead. I don’t worry about bad flowcells with outsourcing
A preprint by @elembio.bsky.social Element Bio on a flowcell-based simplified hybrid capture approach that retains high specificity and enables PCR-free workflow. www.biorxiv.org/content/10.1...
A simplified hybrid capture approach retains high specificity and enables PCR-free workflow
Hybrid capture is a critical technology for selective enrichment of genomic regions of interest, enabling cost-effective focused sequencing in both clinical and research applications. We present a sim...
www.biorxiv.org
March 20, 2025 at 11:30 AM
A preprint by @elembio.bsky.social Element Bio on a flowcell-based simplified hybrid capture approach that retains high specificity and enables PCR-free workflow. www.biorxiv.org/content/10.1...
Cornetto from @hasindu2008.bsky.social presented by Ira Deveson: T2T assembly of human genomes with @nanoporetech.com adaptive sampling by rejecting the "boring bits". 27/46 chromosomes fully assembled from one flowcell. Can also be used on saliva!
#AGTA2025
github.com/hasindu2008/...
#AGTA2025
github.com/hasindu2008/...
October 13, 2025 at 11:53 PM
Cornetto from @hasindu2008.bsky.social presented by Ira Deveson: T2T assembly of human genomes with @nanoporetech.com adaptive sampling by rejecting the "boring bits". 27/46 chromosomes fully assembled from one flowcell. Can also be used on saliva!
#AGTA2025
github.com/hasindu2008/...
#AGTA2025
github.com/hasindu2008/...
You load one @nanoporetech.com flowcell and it almost ruins your day 💩💩💩. You load six more and they look stunning 🟩💚🟩💚💚🟩
October 7, 2025 at 5:10 PM
You load one @nanoporetech.com flowcell and it almost ruins your day 💩💩💩. You load six more and they look stunning 🟩💚🟩💚💚🟩
New preprint online: A flow cell for data validation in computer vision. Computer vision is amazing for reaction monitoring, analytical chemistry, and self-driving laboratories, but it has a flaw… let’s dig deep into this research. #Chemsky 1/n
chemrxiv.org/engage/chemr...
chemrxiv.org/engage/chemr...
May 29, 2025 at 11:07 AM
New preprint online: A flow cell for data validation in computer vision. Computer vision is amazing for reaction monitoring, analytical chemistry, and self-driving laboratories, but it has a flaw… let’s dig deep into this research. #Chemsky 1/n
chemrxiv.org/engage/chemr...
chemrxiv.org/engage/chemr...
Realfreq, a framework for real-time base modification analysis for nanopore sequencing, is now published in Bioinformatics.
Written efficiently using C, a laptop can keep up with a
@nanoporetech.com MinION sequencer, and a desktop a PromethION 2 solo flowcell.
academic.oup.com/bioinformati...
Written efficiently using C, a laptop can keep up with a
@nanoporetech.com MinION sequencer, and a desktop a PromethION 2 solo flowcell.
academic.oup.com/bioinformati...
Realfreq: real-time base modification analysis for nanopore sequencing
AbstractSummary. Nanopore sequencers allow sequencing data to be accessed in real-time. This allows live analysis to be performed, while the sequencing is
academic.oup.com
May 3, 2025 at 4:45 AM
Realfreq, a framework for real-time base modification analysis for nanopore sequencing, is now published in Bioinformatics.
Written efficiently using C, a laptop can keep up with a
@nanoporetech.com MinION sequencer, and a desktop a PromethION 2 solo flowcell.
academic.oup.com/bioinformati...
Written efficiently using C, a laptop can keep up with a
@nanoporetech.com MinION sequencer, and a desktop a PromethION 2 solo flowcell.
academic.oup.com/bioinformati...
This is the flowcell manifold from an Ion Proton. More information on its purpose and construction is available over on the substack (aseq.substack.com)
December 9, 2024 at 6:11 PM
This is the flowcell manifold from an Ion Proton. More information on its purpose and construction is available over on the substack (aseq.substack.com)
In #NGS news, Illumina has started shipping the NovaSeq X Plus 25B flowcell kits bit.ly/40cXg6a
October 30, 2023 at 7:26 PM
In #NGS news, Illumina has started shipping the NovaSeq X Plus 25B flowcell kits bit.ly/40cXg6a
custom flowcell working. it's been a fun build with plenty of learning and problem solving. time to put it to work. Henry's Law is my nemesis
February 16, 2026 at 7:17 PM
custom flowcell working. it's been a fun build with plenty of learning and problem solving. time to put it to work. Henry's Law is my nemesis
Expanding the genome evenly is hard because DNA is a polymer, so here we use the Buenrostro lab's favorite enzyme Tn5 to make fragments beforehand
We then do Illumina sequencing, but instead of on a flowcell, all enzymatics are performed ~inside~ the expanded nucleus
4/
We then do Illumina sequencing, but instead of on a flowcell, all enzymatics are performed ~inside~ the expanded nucleus
4/
November 11, 2024 at 7:33 PM
Expanding the genome evenly is hard because DNA is a polymer, so here we use the Buenrostro lab's favorite enzyme Tn5 to make fragments beforehand
We then do Illumina sequencing, but instead of on a flowcell, all enzymatics are performed ~inside~ the expanded nucleus
4/
We then do Illumina sequencing, but instead of on a flowcell, all enzymatics are performed ~inside~ the expanded nucleus
4/
Yeah, this…if it’s a reused flowcell that’s what I’d worry about but not a big deal otherwise
September 18, 2025 at 6:03 PM
Yeah, this…if it’s a reused flowcell that’s what I’d worry about but not a big deal otherwise
Assembly of purple Catharanthus coming together pretty well from just 1 Nanopore flowcell
July 1, 2024 at 2:48 PM
Assembly of purple Catharanthus coming together pretty well from just 1 Nanopore flowcell
Nanopore reads (1 minIon flowcell, ~10x coverage), assembled with Flye + one round of Medaka polishing. Looking pretty good: BUSCO: C:98.6%[S:97.1%,D:1.5%],F:0.7%,M:0.7%,n:1614
February 14, 2025 at 9:01 PM
Nanopore reads (1 minIon flowcell, ~10x coverage), assembled with Flye + one round of Medaka polishing. Looking pretty good: BUSCO: C:98.6%[S:97.1%,D:1.5%],F:0.7%,M:0.7%,n:1614
For sequencing curious folks: a lab is trying to use up their last NextSeq 550 flowcell, and they're willing to run it for anyone for cost of the flowcell or less (likely a lot less).
Anyone interested in 30~39Gb worth of 150bp reads, let me know! We have about a week to figure this out.
🦠🧫🧪🖥️🧬
Anyone interested in 30~39Gb worth of 150bp reads, let me know! We have about a week to figure this out.
🦠🧫🧪🖥️🧬
December 12, 2024 at 7:31 PM
For sequencing curious folks: a lab is trying to use up their last NextSeq 550 flowcell, and they're willing to run it for anyone for cost of the flowcell or less (likely a lot less).
Anyone interested in 30~39Gb worth of 150bp reads, let me know! We have about a week to figure this out.
🦠🧫🧪🖥️🧬
Anyone interested in 30~39Gb worth of 150bp reads, let me know! We have about a week to figure this out.
🦠🧫🧪🖥️🧬
PacBio HiFi one flowcell, no HiC. Scaffolded onto the closely related K. laxiflora chromosome scale assembly available on JGI. Scaffolding was easy because most chromosomes are single contig already.
December 24, 2024 at 7:47 PM
PacBio HiFi one flowcell, no HiC. Scaffolded onto the closely related K. laxiflora chromosome scale assembly available on JGI. Scaffolding was easy because most chromosomes are single contig already.
We get around 150Gbp of QCed seqences per flowcell, which would yield a coverage of 125x if the genomes are on average 3Mbp and evenly represented.
November 21, 2024 at 4:57 PM
We get around 150Gbp of QCed seqences per flowcell, which would yield a coverage of 125x if the genomes are on average 3Mbp and evenly represented.
$BNGO - Bionano
Q3 2024 Earnings Call - (Part II - the details)
A) 5 sales per quarter is the current guidance going forward. Erik Holmlin was categorical when asked the question - the emphasis is on flowcell sales, management aims to be ultra prudent in its guidance at all times.
1/11
Q3 2024 Earnings Call - (Part II - the details)
A) 5 sales per quarter is the current guidance going forward. Erik Holmlin was categorical when asked the question - the emphasis is on flowcell sales, management aims to be ultra prudent in its guidance at all times.
1/11
November 19, 2024 at 8:20 PM
$BNGO - Bionano
Q3 2024 Earnings Call - (Part II - the details)
A) 5 sales per quarter is the current guidance going forward. Erik Holmlin was categorical when asked the question - the emphasis is on flowcell sales, management aims to be ultra prudent in its guidance at all times.
1/11
Q3 2024 Earnings Call - (Part II - the details)
A) 5 sales per quarter is the current guidance going forward. Erik Holmlin was categorical when asked the question - the emphasis is on flowcell sales, management aims to be ultra prudent in its guidance at all times.
1/11