#InVivoReprogramming
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Sharing a visualization from a methodological exercise on partial reprogramming kinetics that may be of interest to colleagues working on in vivo epigenetic rejuvenation paradigms.

#PartialReprogramming #YamanakaFactors #EpigeneticRejuvenation #AgingBiology #InVivoReprogramming
Sharing a visualization from a methodological exercise on partial reprogramming kinetics that may be of interest to colleagues working on in vivo epigenetic rejuvenation paradigms. **Figure:** Transient OSKM Induction Kinetics in Doxycycline-Pulsed Reprogrammable Mice **Subtitle:** Relative transcript abundance of each Yamanaka factor over a 7-day induction–withdrawal cycle in liver The multiline plot tracks normalized mRNA levels of Oct4 (Pou5f1), Sox2, Klf4, and c-Myc across a canonical short-cycle doxycycline regimen in a tetO-OSKM; Rosa26-M2rtTA background, with hepatic tissue as the sampled compartment. The temporal window spans a 48-hour dox administration phase followed by a 5-day washout, capturing induction onset, transgene saturation, and post-withdrawal decay. Key features rendered in the visualization: - Rapid transactivation of all four cassettes within 12–24 h post-dox, with c-Myc and Klf4 displaying the steepest initial slopes, consistent with their reported transcriptional responsiveness and shorter transcript half-lives. - Asynchronous peak amplitudes: Oct4 and Sox2 reach maxima at ~36–48 h, while c-Myc plateaus earlier, reflecting differential polycistronic cassette dynamics and endogenous locus contributions. - Post-withdrawal decay follows factor-specific kinetics; c-Myc transcripts return to near-baseline within 48 h of dox removal, whereas Oct4 exhibits a more protracted decline — relevant when designing cyclic regimens intended to avoid teratoma risk while sustaining epigenetic remodeling. - Absence of overt lineage-inappropriate marker induction within the plotted window, consistent with the sub-dedifferentiation threshold characteristic of partial reprogramming protocols (cf. Ocampo et al., 2016; Browder et al., 2022; Chondronasiou et al., 2022).
July 10, 2026 at 5:03 PM