Another #Lifeact model system. the quail is still my favourite though… #cytoskeleton @melaniedwhite.bsky.social
rupress.org/jcb/article/...
rupress.org/jcb/article/...
November 5, 2026 at 8:48 AM
Another #Lifeact model system. the quail is still my favourite though… #cytoskeleton @melaniedwhite.bsky.social
rupress.org/jcb/article/...
rupress.org/jcb/article/...
A preprint using: pLenti-Lifeact-tdTom (RRID:Addgene_64048) from @addgene.bsky.social was published.
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #RRID #reproducibility
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #RRID #reproducibility
www.biorxiv.org
November 1, 2026 at 1:00 PM
A preprint using: pLenti-Lifeact-tdTom (RRID:Addgene_64048) from @addgene.bsky.social was published.
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #RRID #reproducibility
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #RRID #reproducibility
How curious? This myeloid cell, even with its chromatins completely condensed, refuses to complete its mitosis. I thought this was my chance to image its division, but after awhile, it just moved away from me, even with its chromosomes still condensed! 🔵=MLC, 💜=H2B+LifeAct.
#FluorescenceFriday
#FluorescenceFriday
October 22, 2026 at 11:35 PM
How curious? This myeloid cell, even with its chromatins completely condensed, refuses to complete its mitosis. I thought this was my chance to image its division, but after awhile, it just moved away from me, even with its chromosomes still condensed! 🔵=MLC, 💜=H2B+LifeAct.
#FluorescenceFriday
#FluorescenceFriday
Happy #MicroscopyMonday.
I found this very pretty COS-7 cell expressing LifeAct-mCherry from my old data. Look at how dense its actin network is. Also, many of the moving dots are endosomal to seemingly push them through the cytoplasm, similar to actin comet tail, but just a lot shorter.
I found this very pretty COS-7 cell expressing LifeAct-mCherry from my old data. Look at how dense its actin network is. Also, many of the moving dots are endosomal to seemingly push them through the cytoplasm, similar to actin comet tail, but just a lot shorter.
September 21, 2026 at 6:28 PM
Happy #MicroscopyMonday.
I found this very pretty COS-7 cell expressing LifeAct-mCherry from my old data. Look at how dense its actin network is. Also, many of the moving dots are endosomal to seemingly push them through the cytoplasm, similar to actin comet tail, but just a lot shorter.
I found this very pretty COS-7 cell expressing LifeAct-mCherry from my old data. Look at how dense its actin network is. Also, many of the moving dots are endosomal to seemingly push them through the cytoplasm, similar to actin comet tail, but just a lot shorter.
Cool!
HeLa cell with lifeAct, homebuilt 3D SIM.
(imaged 14 years ago in Gustafsson lab).
HeLa cell with lifeAct, homebuilt 3D SIM.
(imaged 14 years ago in Gustafsson lab).
September 9, 2026 at 1:22 AM
Cool!
HeLa cell with lifeAct, homebuilt 3D SIM.
(imaged 14 years ago in Gustafsson lab).
HeLa cell with lifeAct, homebuilt 3D SIM.
(imaged 14 years ago in Gustafsson lab).
I'm willing - grudgingly - to accept that Lifeact is not a valid pangram in today's Spelling Bee, but what's wrong with felicitate?!
September 8, 2026 at 4:04 PM
I'm willing - grudgingly - to accept that Lifeact is not a valid pangram in today's Spelling Bee, but what's wrong with felicitate?!
We confirmed this switch using a bunch of different techniques, from live imaging of transgenic choanos expressing an F-actin marker (LifeAct) to ultrastructural expansion microscopy, and the story was always the same: no actin cortex in flagellates, but a clear cortex after confinement.
September 2, 2026 at 9:19 PM
We confirmed this switch using a bunch of different techniques, from live imaging of transgenic choanos expressing an F-actin marker (LifeAct) to ultrastructural expansion microscopy, and the story was always the same: no actin cortex in flagellates, but a clear cortex after confinement.
#cytoskeleton
So, there is a small (very small) 9-aa motif that is an evolutionary conserved anchor to actin.
It's named: short linear actin filament-binding motif (SFM), and it's *the same* found in LifeAct !
Full: www.nature.com/articles/s41... @natcellbio.nature.com @cellclub.bsky.social 🧪🔬
So, there is a small (very small) 9-aa motif that is an evolutionary conserved anchor to actin.
It's named: short linear actin filament-binding motif (SFM), and it's *the same* found in LifeAct !
Full: www.nature.com/articles/s41... @natcellbio.nature.com @cellclub.bsky.social 🧪🔬
August 18, 2026 at 9:32 PM
#cytoskeleton
So, there is a small (very small) 9-aa motif that is an evolutionary conserved anchor to actin.
It's named: short linear actin filament-binding motif (SFM), and it's *the same* found in LifeAct !
Full: www.nature.com/articles/s41... @natcellbio.nature.com @cellclub.bsky.social 🧪🔬
So, there is a small (very small) 9-aa motif that is an evolutionary conserved anchor to actin.
It's named: short linear actin filament-binding motif (SFM), and it's *the same* found in LifeAct !
Full: www.nature.com/articles/s41... @natcellbio.nature.com @cellclub.bsky.social 🧪🔬
Kai wanted me to pass on his answer, it's LifeAct 😁
August 18, 2026 at 3:46 PM
Kai wanted me to pass on his answer, it's LifeAct 😁
Thanks, Pablo. This is an early macrophage and its LifeAct 😁
June 6, 2026 at 8:32 AM
Thanks, Pablo. This is an early macrophage and its LifeAct 😁
Happy #FluorescenceFriday!
I captured this shot of a group of embryonic macrophages expressing LifeAct and H2B migrating inside tissue. These cells can use lamellipodia, filopodia, or blebs to migrate, and are frequently switching between these different modes or combining them. So fascinating.
I captured this shot of a group of embryonic macrophages expressing LifeAct and H2B migrating inside tissue. These cells can use lamellipodia, filopodia, or blebs to migrate, and are frequently switching between these different modes or combining them. So fascinating.
May 21, 2026 at 11:31 PM
Happy #FluorescenceFriday!
I captured this shot of a group of embryonic macrophages expressing LifeAct and H2B migrating inside tissue. These cells can use lamellipodia, filopodia, or blebs to migrate, and are frequently switching between these different modes or combining them. So fascinating.
I captured this shot of a group of embryonic macrophages expressing LifeAct and H2B migrating inside tissue. These cells can use lamellipodia, filopodia, or blebs to migrate, and are frequently switching between these different modes or combining them. So fascinating.
Haha thank you so much. This is just LifeAct-RFP. The cell is COS7. I used Airyscan 880 to make this video.
November 30, 2024 at 10:10 AM
Haha thank you so much. This is just LifeAct-RFP. The cell is COS7. I used Airyscan 880 to make this video.
Arittri's amazing finding that our old friend Lifeact can make actomyosin flow in the opposite direction, change chirality and flip left and right in the C.elegans worm.
Check out here for more details:
www.biorxiv.org/content/10.6...
Check out here for more details:
www.biorxiv.org/content/10.6...
February 20, 2026 at 4:48 PM
Arittri's amazing finding that our old friend Lifeact can make actomyosin flow in the opposite direction, change chirality and flip left and right in the C.elegans worm.
Check out here for more details:
www.biorxiv.org/content/10.6...
Check out here for more details:
www.biorxiv.org/content/10.6...
Some old unpublished images of LifeAct T cells spreading on various activating or non-activating surfaces.
December 10, 2025 at 6:42 PM
Some old unpublished images of LifeAct T cells spreading on various activating or non-activating surfaces.
#MicroscopyMonday Disco cell! One more RFP-LifeAct cell on top of nanoneedles. I believe these needles are actually a polymer cast of a cast of an Si array. I love the way the cell is exploring the topography with actin-rich protrusions that look like little octopus suckers. (5 min/frame)
November 25, 2024 at 7:12 PM
#MicroscopyMonday Disco cell! One more RFP-LifeAct cell on top of nanoneedles. I believe these needles are actually a polymer cast of a cast of an Si array. I love the way the cell is exploring the topography with actin-rich protrusions that look like little octopus suckers. (5 min/frame)
#MicroscopyMonday Epithelial cells expressing RFP-LifeAct on an upside-down Si wafer. I used silicone grease to hold the wafer down on a 3D-printed support, and some spots of grease got on the wafer. These tiny bumps show up as bright rings of actin where the cells crawl over them! Fun accident.
November 25, 2024 at 6:42 PM
#MicroscopyMonday Epithelial cells expressing RFP-LifeAct on an upside-down Si wafer. I used silicone grease to hold the wafer down on a 3D-printed support, and some spots of grease got on the wafer. These tiny bumps show up as bright rings of actin where the cells crawl over them! Fun accident.
To characterize these dynamics, we generated a new transgenic quail line expressing fluorescent reporters for the myosin regulatory light chain (MYL9) and F-actin (LifeAct). In both the epiblast and the PS, the reporters are strongly enriched at epithelial junctions. (2/n)
December 19, 2025 at 4:33 PM
To characterize these dynamics, we generated a new transgenic quail line expressing fluorescent reporters for the myosin regulatory light chain (MYL9) and F-actin (LifeAct). In both the epiblast and the PS, the reporters are strongly enriched at epithelial junctions. (2/n)
Interesting how the leading edge of the cell front is lifeact-
April 10, 2026 at 5:09 PM
Interesting how the leading edge of the cell front is lifeact-
An ascidian egg dividing - as seen with fluorescent LifeAct protein (labels actin). The cleavage ring becoming smaller. Thanks to the Ascidian BioCell Team at LBDV
December 3, 2024 at 3:14 PM
An ascidian egg dividing - as seen with fluorescent LifeAct protein (labels actin). The cleavage ring becoming smaller. Thanks to the Ascidian BioCell Team at LBDV
Witzig. 95 war bei mir das Jahr, in dem es bei mir mit Goa so richtig los ging. Ich hatte nach Leuten um einen Rave-Lifeact zu gründen gesucht und auf einmal bestand meine Playlist zu 80% aus Zeugs von SpiritZone.
Die VooV-Experience 4 hat mich so verändert *_*
Die VooV-Experience 4 hat mich so verändert *_*
May 8, 2025 at 5:44 AM
Witzig. 95 war bei mir das Jahr, in dem es bei mir mit Goa so richtig los ging. Ich hatte nach Leuten um einen Rave-Lifeact zu gründen gesucht und auf einmal bestand meine Playlist zu 80% aus Zeugs von SpiritZone.
Die VooV-Experience 4 hat mich so verändert *_*
Die VooV-Experience 4 hat mich so verändert *_*
Then, we extended CALIPERS to human stem cells by expressing FUCCIplex and LifeAct in
Bruce Conklin's GCaMP6f-WTC11 hiPSCs reference line (parental line of the @alleninstitute.bsky.social Cell Collection).
Bruce Conklin's GCaMP6f-WTC11 hiPSCs reference line (parental line of the @alleninstitute.bsky.social Cell Collection).
January 17, 2025 at 4:09 PM
Then, we extended CALIPERS to human stem cells by expressing FUCCIplex and LifeAct in
Bruce Conklin's GCaMP6f-WTC11 hiPSCs reference line (parental line of the @alleninstitute.bsky.social Cell Collection).
Bruce Conklin's GCaMP6f-WTC11 hiPSCs reference line (parental line of the @alleninstitute.bsky.social Cell Collection).
FUCCIplex replaces GFP/RFP with iRFP & mTurquoise2, allowing multiplexed imaging of CC with standard fluorescence microscopes. See it in action tracking HaCaT cells across 40 hours (LifeAct in grey).
January 17, 2025 at 4:09 PM
FUCCIplex replaces GFP/RFP with iRFP & mTurquoise2, allowing multiplexed imaging of CC with standard fluorescence microscopes. See it in action tracking HaCaT cells across 40 hours (LifeAct in grey).
For your viewing pleasure, enjoy watching these cannibalistic podocytes eating the remnants of their dead sibling.
immortalized human podocytes expressing mClover-lifeact
immortalized human podocytes expressing mClover-lifeact
May 12, 2025 at 10:22 PM
For your viewing pleasure, enjoy watching these cannibalistic podocytes eating the remnants of their dead sibling.
immortalized human podocytes expressing mClover-lifeact
immortalized human podocytes expressing mClover-lifeact
What is happening with these differentiating adipocyte-derived stem cells??? 🤯 Why is EGFP-LifeAct in/around the nucleus? Artifact?? Why would it disappear while others are stable for hours? #FluorescenceFriday #Actin
May 8, 2026 at 3:03 PM
What is happening with these differentiating adipocyte-derived stem cells??? 🤯 Why is EGFP-LifeAct in/around the nucleus? Artifact?? Why would it disappear while others are stable for hours? #FluorescenceFriday #Actin