#MAD7
結婚しましょ♪そうしましょ♪こまひMAD7個目みーつけた
August 28, 2026 at 11:26 AM
🤖 Expanding the CRISPR toolbox for Chinese hamster ovary cells with comprehensive tools for Mad7 genome editing.
Biotechnology and bioengineering(2023)
#Biotechnology #Science
https://pubmed.ncbi.nlm.nih.gov/36864663/
pubmed.ncbi.nlm.nih.gov
August 17, 2026 at 6:42 AM
Gemini要約🤖CHO細胞でCRISPR/Cas12a系酵素Mad7を用いたゲノム編集技術を確立。NLS付加等の最適化で効率的なノックアウトが可能です。設計・解析ツールも提供し、細胞構築の選択肢を広げる成果です。
August 17, 2026 at 6:42 AM
ギャグ日のMADだとこれが大好きだからぜひ見てほしい
音ハメ気持ち良い

www.nicovideo.jp/watch/sm9272...
【MAD】ギャグマンガ日和で君の知らない物語【まさかのFULL】
【MAD】ギャグマンガ日和で君の知らない物語【まさかのFULL】 [アニメ] MAD7作目。前作(再うp→sm13330729)のED、まさかのFULLバージョン。ちなみにギャグマンガ日和1~3期...
www.nicovideo.jp
December 4, 2024 at 10:40 AM
@wolftwinthomas.bsky.social

Please read you follow paulst001 and interact with them. He is not what he seems.

The second post in this thread is screenshots.

Trying to keep the community safe, please read and share.

1/2
bsky.app/profile/mad7...
I’ve just seen something… if you follow this thing!! And I was.. blocking now, maybe block as well.. some comments I just read regarding woman were pure filth!
Be respectful please or you will be named!! 😡📷🦋
November 10, 2025 at 7:51 PM
@arik-2.bsky.social

Please read you follow paulst001 and interact with them. He is not what he seems.

The second post in this thread is screenshots.

Trying to keep the community safe, please read and share.

1/2
bsky.app/profile/mad7...
I’ve just seen something… if you follow this thing!! And I was.. blocking now, maybe block as well.. some comments I just read regarding woman were pure filth!
Be respectful please or you will be named!! 😡📷🦋
November 10, 2025 at 5:55 PM
@cn2409.bsky.social

Please read you follow paulst001 and interact with them. He is not what he seems.

The second post in this thread is screenshots.

Trying to keep the community safe, please read and share.

1/2
bsky.app/profile/mad7...
I’ve just seen something… if you follow this thing!! And I was.. blocking now, maybe block as well.. some comments I just read regarding woman were pure filth!
Be respectful please or you will be named!! 😡📷🦋
November 10, 2025 at 5:53 PM
@vmc54.bsky.social

Please read you follow paulst001 and interact with them. He is not what he seems.

The second post in this thread is screenshots.

Trying to keep the community safe, please read and share.

1/2
bsky.app/profile/mad7...
I’ve just seen something… if you follow this thing!! And I was.. blocking now, maybe block as well.. some comments I just read regarding woman were pure filth!
Be respectful please or you will be named!! 😡📷🦋
November 10, 2025 at 5:52 PM
@sabineshutterbug.bsky.social

Please read you follow paulst001 and interact with them. He is not what he seems.

The second post in this thread is screenshots.

Trying to keep the community safe, please read and share.

1/2
bsky.app/profile/mad7...
I’ve just seen something… if you follow this thing!! And I was.. blocking now, maybe block as well.. some comments I just read regarding woman were pure filth!
Be respectful please or you will be named!! 😡📷🦋
November 10, 2025 at 5:51 PM
@honeybeastie.bsky.social

Please read you follow paulst001 and interact with them. He is not what he seems.

The second post in this thread is screenshots.

Trying to keep the community safe, please read and share.

1/2
bsky.app/profile/mad7...
I’ve just seen something… if you follow this thing!! And I was.. blocking now, maybe block as well.. some comments I just read regarding woman were pure filth!
Be respectful please or you will be named!! 😡📷🦋
November 10, 2025 at 5:48 PM
@squarebizz.bsky.social

Please read you follow paulst001 and interact with them. He is not what he seems.

The second post in this thread is screenshots plus community warning.

Trying to keep the community safe, please read and share.

1/2
bsky.app/profile/mad7...
I’ve just seen something… if you follow this thing!! And I was.. blocking now, maybe block as well.. some comments I just read regarding woman were pure filth!
Be respectful please or you will be named!! 😡📷🦋
November 9, 2025 at 8:23 PM
@ombluenose.bsky.social

Please read you follow paulst001 and interact with them. He is not what he seems.

The second post in this thread is screenshots plus community warning.

Trying to keep the community safe, please read and share.

1/2
bsky.app/profile/mad7...
I’ve just seen something… if you follow this thing!! And I was.. blocking now, maybe block as well.. some comments I just read regarding woman were pure filth!
Be respectful please or you will be named!! 😡📷🦋
November 9, 2025 at 8:16 PM
@misshistoryg2.bsky.social

Please read you follow paulst001 and interact with them. He is not what he seems.

The second post in this thread is screenshots plus community warning.

Trying to keep the community safe, please read and share.

1/2
bsky.app/profile/mad7...
I’ve just seen something… if you follow this thing!! And I was.. blocking now, maybe block as well.. some comments I just read regarding woman were pure filth!
Be respectful please or you will be named!! 😡📷🦋
November 9, 2025 at 8:13 PM
@evalilliekvist.bsky.social

Please read you follow paulst001 and interact with them. He is not what he seems.

The second post in this thread is screenshots plus community warning.

Trying to keep the community safe, please read and share.

1/2
bsky.app/profile/mad7...
I’ve just seen something… if you follow this thing!! And I was.. blocking now, maybe block as well.. some comments I just read regarding woman were pure filth!
Be respectful please or you will be named!! 😡📷🦋
November 9, 2025 at 7:57 PM
Blessed be thy Monday

Sporcery, new album by Sporcerer, has arrived
spaceseer.band-camp.com/album/sporcery

Album artwork by @dronedartworks.bsky.social

9jal-343q
nbfj-hmdz
mad7-5twg
g9ja-bbl3
y3br-xxqs
2vvw-e5gd
d9gq-7rrh
stuf-54hv
jjgh-6tap
7buh-ynu2

#comfysynth #dungeonsynth #synthesizer
January 26, 2026 at 2:27 PM
➡️ El @gobjccm.bsky.social traslada su apoyo a las empresas que han apostado por instalarse en Castilla-La Mancha adoptando criterios de sostenibilidad

✅Gómez ha visitado el centro logístico de #Amazon MAD7
👉 ✍️

www.castillalamancha.es/node/407433
September 8, 2025 at 12:28 PM
買い物

ポーさん1巻
MAD7巻
May 1, 2026 at 1:10 AM
I spoke to 700 of the world's top chefs in Copenhagen recently at MAD7, the food symposium created by Noma restaraunt founder Rene Redzepi. This is what I told them about the art of long-term thinking and the 100-year-old Japanese robe I'm wearing.
video.madfeed.co/how-can-we-b...
How Can We Be Good Ancestors? | Roman Krznaric MAD 7
When philosopher Roman Krznaric voted in a UK election, he gave his ballot to his kids. His message was clear: their futures will be shaped by today’s...
video.madfeed.co
July 2, 2025 at 10:41 AM
The authors included RRIDs in their in In Vitro Cellular & Developmental Biology - Plant paper! Thanks for making your methods matter! #BetterScience #RRID #ReproducibleResearch
MAD7-mediated genome editing via Agrobacterium enables recovery of hexa-allelic edited lines carrying distinct mutations in all six gene copies in wheat (Triticum aestivum L.) T0 plants and stable inheritance of edits into the following generation - In Vitro Cellular & Developmental Biology
The expansion of genome-editing tools for bread wheat (Triticum aestivum L.) is essential to accelerate trait improvement while addressing intellectual property constraints associated with widely used CRISPR systems. MAD7 (ErCas12a), a royalty-accessible CRISPR nuclease, represents a potential alternative to CRISPR-Cas9; however, its performance in complex polyploid crops remains insufficiently characterized. In this study, the in planta genome-editing efficiency of MAD7 in hexaploid wheat was evaluated using Agrobacterium-mediated transformation. Conserved coding regions of the TaLCYε (LYCOPENE EPSILON CYCLASE) gene, a key regulator of carotenoid flux, were targeted across all three wheat subgenomes (A, B, and D). MAD7-mediated editing showed strong dependence on protospacer adjacent motif (PAM) composition, with detectable activity only at a T-rich PAM (TTTG), resulting in targeted mutations in 26% of transgenic T0 plants. Notably, MAD7 enabled recovery of hexa-allelic edited li
doi.org
July 8, 2026 at 7:00 AM
RRIDs were included in this paper. We value the author's support of reproducibility. #reproducibility #RRID #OpenScience
Engineering a High-Fidelity MAD7 Variant with Enhanced Specificity for Precision Genome Editing via CcdB-Based Bacterial Screening
doi.org
November 27, 2025 at 8:02 AM
More vintage shortwave pirate QSLs! www.youtube.com/watch?v=maD7...

Do you have some of your own pirate radio QSLs, or want to see QSLs you've posted here to the HFU featured on an upcoming video? Contact me!
Shortwave Pirate Radio QSLs - Episode 3
YouTube video by HFUnderground
www.youtube.com
September 25, 2025 at 7:01 PM
Not long now, and these beautiful #Raptors start flying again, for 2025 🦉😎 #SuffolkOwls U.K.
🎶Journey 🎶🎸 📷🌱🌳☀️
💃🏻🎶’Don’t Stop Believin’🎶🕺🏻
#Owls #Birds #Nature
March 10, 2025 at 6:20 AM
Well done Alina, I had 5 mins so I did mine 9.8 ...I should try harder 😂.. Enjoy the rest of your day 🌿🦉🎶💚🌳☀️👋
Check out these Bluesky follower stats: bluefacts.app/profile/mad7...
Mark D Alexander (@mad7500.bsky.social) - Bluesky User Insights
Mark D Alexander has a CloudRank of 9.8 out of 10. Check out your own Bluesky influence score on BlueFacts!
bluefacts.app
August 6, 2026 at 8:10 PM