Microfluidic #BloodBrainBarrier
hiPSC Endothelial Cells or primary brain microvascular ECs +primary brain pericytes & astrocytes
A protocol for microscopy-based permeability assay (minutes)
10/40 kD dextran
#NatProtoc 2022
www.nature.com/articles/s41...
hiPSC Endothelial Cells or primary brain microvascular ECs +primary brain pericytes & astrocytes
A protocol for microscopy-based permeability assay (minutes)
10/40 kD dextran
#NatProtoc 2022
www.nature.com/articles/s41...
October 20, 2025 at 7:06 PM
Microfluidic #BloodBrainBarrier
hiPSC Endothelial Cells or primary brain microvascular ECs +primary brain pericytes & astrocytes
A protocol for microscopy-based permeability assay (minutes)
10/40 kD dextran
#NatProtoc 2022
www.nature.com/articles/s41...
hiPSC Endothelial Cells or primary brain microvascular ECs +primary brain pericytes & astrocytes
A protocol for microscopy-based permeability assay (minutes)
10/40 kD dextran
#NatProtoc 2022
www.nature.com/articles/s41...
NicheNet v2
zenodo.org/records/7074...
Resolve cell-cell communications with ~5k ligand-receptor pairs
Account for DEG in receiver cells
Sender/ligand-agnostic + Sender-focused approach
A step-by-step protocol😎
#NatProtoc 2025
www.nature.com/articles/s41...
zenodo.org/records/7074...
Resolve cell-cell communications with ~5k ligand-receptor pairs
Account for DEG in receiver cells
Sender/ligand-agnostic + Sender-focused approach
A step-by-step protocol😎
#NatProtoc 2025
www.nature.com/articles/s41...
May 3, 2026 at 12:10 PM
NicheNet v2
zenodo.org/records/7074...
Resolve cell-cell communications with ~5k ligand-receptor pairs
Account for DEG in receiver cells
Sender/ligand-agnostic + Sender-focused approach
A step-by-step protocol😎
#NatProtoc 2025
www.nature.com/articles/s41...
zenodo.org/records/7074...
Resolve cell-cell communications with ~5k ligand-receptor pairs
Account for DEG in receiver cells
Sender/ligand-agnostic + Sender-focused approach
A step-by-step protocol😎
#NatProtoc 2025
www.nature.com/articles/s41...
An accessible workflow for high-sensitivity proteomics using parallel accumulation–serial fragmentation (PASEF) #NatProtoc www.nature.com/articles/s41...
An accessible workflow for high-sensitivity proteomics using parallel accumulation–serial fragmentation (PASEF) - Nature Protocols
Aligning trapped ion mobility with a mass-selective quadrupole and time-of-flight mass spectrometry enables parallel accumulation–serial fragmentation, which improves proteomic analysis. This protocol...
www.nature.com
January 19, 2025 at 1:26 PM
An accessible workflow for high-sensitivity proteomics using parallel accumulation–serial fragmentation (PASEF) #NatProtoc www.nature.com/articles/s41...
High-throughput reaction screening using acoustic ejection mass spectrometry #NatProtoc www.nature.com/articles/s41...
High-throughput reaction screening using acoustic ejection mass spectrometry - Nature Protocols
A bottleneck in high-throughput chemical synthesis is analyzing reaction products. This protocol describes a direct mass spectrometry approach where the loss of a characteristic neutral fragment from ...
www.nature.com
February 24, 2026 at 9:19 PM
High-throughput reaction screening using acoustic ejection mass spectrometry #NatProtoc www.nature.com/articles/s41...
A guide to reverse metabolomics-a framework for big data discovery strategy #NatProtoc www.nature.com/articles/s41...
A guide to reverse metabolomics—a framework for big data discovery strategy - Nature Protocols
In this reverse metabolomics protocol, a tandem mass spectrometry spectrum is used as a search term to probe public metabolomic data. Analysis of the metadata connected with these search results enabl...
www.nature.com
March 1, 2025 at 8:11 PM
A guide to reverse metabolomics-a framework for big data discovery strategy #NatProtoc www.nature.com/articles/s41...
Direct and quantitative analysis of tRNA acylation using intact tRNA liquid chromatography-mass spectrometry #NatProtoc www.nature.com/articles/s41...
Direct and quantitative analysis of tRNA acylation using intact tRNA liquid chromatography–mass spectrometry - Nature Protocols
With this quantitative liquid chromatography–mass spectrometry-based assay designed for the detection of intact acyl-tRNA products, users can accurately discriminate acyl from non-acyl-tRNA, identify ...
www.nature.com
January 7, 2025 at 7:19 PM
Direct and quantitative analysis of tRNA acylation using intact tRNA liquid chromatography-mass spectrometry #NatProtoc www.nature.com/articles/s41...
Improving accuracy and reproducibility of mass spectrometry characterization of protein coronas on nanoparticles #NatProtoc www.nature.com/articles/s41...
Improving accuracy and reproducibility of mass spectrometry characterization of protein coronas on nanoparticles - Nature Protocols
Nanoparticle pharmacokinetics is affected by the formation of a protein corona in vivo. Mass spectrometry-based proteomics is essential for understanding the corona composition. This Perspective discu...
www.nature.com
June 12, 2025 at 10:47 AM
Improving accuracy and reproducibility of mass spectrometry characterization of protein coronas on nanoparticles #NatProtoc www.nature.com/articles/s41...
A versatile toolkit for drug metabolism studies with GNPS2: from drug development to clinical monitoring #NatProtoc #MassSpec www.nature.com/articles/s41...
A versatile toolkit for drug metabolism studies with GNPS2: from drug development to clinical monitoring - Nature Protocols
This protocol extension guides users in identifying candidate drug metabolites without prior knowledge by analyzing tandem mass spectrometry data in the web-based molecular networking tool GNPS2.
www.nature.com
September 9, 2025 at 7:52 AM
A versatile toolkit for drug metabolism studies with GNPS2: from drug development to clinical monitoring #NatProtoc #MassSpec www.nature.com/articles/s41...
Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers #NatProtoc www.nature.com/articles/s41...
Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers - Nature Protocols
Orbitrap-based individual ion mass spectrometry enables charge detection mass spectrometry application on a broadly accessible mass spectrometry platform, enabling the analysis of complex mixtures tha...
www.nature.com
January 3, 2025 at 4:12 PM
Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers #NatProtoc www.nature.com/articles/s41...
PEPPI-MS: gel-based sample pre-fractionation for deep top-down and middle-down proteomics #NatProtoc www.nature.com/articles/s41...
PEPPI-MS: gel-based sample pre-fractionation for deep top-down and middle-down proteomics - Nature Protocols
This protocol outlines a cost-effective and rapid protein sample pre-fractionation strategy based on highly efficient passive elution from gels, recovering intact proteins for top-down proteomics or p...
www.nature.com
January 18, 2025 at 4:29 PM
PEPPI-MS: gel-based sample pre-fractionation for deep top-down and middle-down proteomics #NatProtoc www.nature.com/articles/s41...
Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level #NatProtoc www.nature.com/articles/s41...
Client Challenge
www.nature.com
January 14, 2026 at 7:45 PM
Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level #NatProtoc www.nature.com/articles/s41...
Proteome-wide profiling of S-nitrosylated proteins using the SNOTRAP probe and mass spectrometry-based detection #NatProtoc www.nature.com/articles/s41...
Proteome-wide profiling of S-nitrosylated proteins using the SNOTRAP probe and mass spectrometry-based detection - Nature Protocols
This robust, proteome-wide approach for the exploration of the S-nitrosoproteome in human and mouse tissues uses the SNOTRAP probe and nano-liquid chromatography–tandem mass spectrometry analysis. The...
www.nature.com
November 19, 2025 at 8:56 PM
Proteome-wide profiling of S-nitrosylated proteins using the SNOTRAP probe and mass spectrometry-based detection #NatProtoc www.nature.com/articles/s41...
Metabolomics using anion-exchange chromatography mass spectrometry for the analysis of cells, tissues and biofluids #NatProtoc #MassSpec www.nature.com/articles/s41...
Metabolomics using anion-exchange chromatography mass spectrometry for the analysis of cells, tissues and biofluids - Nature Protocols
We present a protocol for the analysis of polar and ionic metabolites by using anion-exchange chromatography–tandem mass spectrometry (AEC-MS/MS) with an untargeted or semi-targeted workflow for the i...
www.nature.com
August 23, 2025 at 2:28 PM
Metabolomics using anion-exchange chromatography mass spectrometry for the analysis of cells, tissues and biofluids #NatProtoc #MassSpec www.nature.com/articles/s41...
Mass spectrometry-based top-down proteomics for proteoform profiling of protein coronas #NatProtoc www.nature.com/articles/s41...
Mass spectrometry-based top-down proteomics for proteoform profiling of protein coronas - Nature Protocols
It is important to understand how nanoparticles interact with biological systems and behave in vivo. This protocol describes top-down proteomics for protein corona profiling using advanced capillary z...
www.nature.com
August 6, 2025 at 6:53 AM
Mass spectrometry-based top-down proteomics for proteoform profiling of protein coronas #NatProtoc www.nature.com/articles/s41...
High-spatial-resolution mass spectrometry imaging of biological tissues using a microfluidic probe #NatProtoc www.nature.com/articles/s41...
High-spatial-resolution mass spectrometry imaging of biological tissues using a microfluidic probe - Nature Protocols
We present a protocol for the design, fabrication and use of a microfluidic probe for nanospray desorption electrospray ionization mass spectrometry imaging (nano-DESI MSI) that achieves a spatial res...
www.nature.com
June 13, 2025 at 9:35 AM
High-spatial-resolution mass spectrometry imaging of biological tissues using a microfluidic probe #NatProtoc www.nature.com/articles/s41...
Direct analysis of biotransformations with mass spectrometry—DiBT-MS #NatProtoc www.nature.com/articles/s41...
Direct analysis of biotransformations with mass spectrometry—DiBT-MS - Nature Protocols
Engineering enzymes capable of performing chemical transformations requires high-throughput assays to screen activity. In this protocol, reactions in cell lysates are analyzed directly by desorption e...
www.nature.com
April 22, 2025 at 5:43 PM
Direct analysis of biotransformations with mass spectrometry—DiBT-MS #NatProtoc www.nature.com/articles/s41...