Our updated study is now published in Genes & Development! We set out to better understand how DAXX assembles H3.3 nucleosomes. This led us to identify separation-of-function mutants that allowed us to disentangle H3.3 deposition from ERV repression.
genesdev.cshlp.org/content/earl...
genesdev.cshlp.org/content/earl...
November 4, 2026 at 10:22 PM
Our updated study is now published in Genes & Development! We set out to better understand how DAXX assembles H3.3 nucleosomes. This led us to identify separation-of-function mutants that allowed us to disentangle H3.3 deposition from ERV repression.
genesdev.cshlp.org/content/earl...
genesdev.cshlp.org/content/earl...
Finding the right gene to switch on is a needle-in-a-haystack problem, except the haystack is wrapped in nucleosomes.
Using minichromosomes, Wei et al. (2026) built a way to track single molecules of transcription factor GCN4 as it searched for its target gene.
📄 www.nature.com/articles/s41...
Using minichromosomes, Wei et al. (2026) built a way to track single molecules of transcription factor GCN4 as it searched for its target gene.
📄 www.nature.com/articles/s41...
November 4, 2026 at 5:15 PM
Finding the right gene to switch on is a needle-in-a-haystack problem, except the haystack is wrapped in nucleosomes.
Using minichromosomes, Wei et al. (2026) built a way to track single molecules of transcription factor GCN4 as it searched for its target gene.
📄 www.nature.com/articles/s41...
Using minichromosomes, Wei et al. (2026) built a way to track single molecules of transcription factor GCN4 as it searched for its target gene.
📄 www.nature.com/articles/s41...
The last part of my PhD work is on bioRxiv! Using Cryo-EM, optical tweezers, and in-cell footprinting, we showed that FoxO1 and HNF1α TFs engage and cooperate to bind nucleosomes in vitro and in native chromatin, modulating its architecture and stability. www.biorxiv.org/content/10.6...
| bioRxiv
bioRxiv - the preprint server for biology, operated by openRxiv, a nonprofit organization dedicated to advancing scientific communication
www.biorxiv.org
November 3, 2026 at 9:40 PM
The last part of my PhD work is on bioRxiv! Using Cryo-EM, optical tweezers, and in-cell footprinting, we showed that FoxO1 and HNF1α TFs engage and cooperate to bind nucleosomes in vitro and in native chromatin, modulating its architecture and stability. www.biorxiv.org/content/10.6...
Epigenetics Update - PARP1 directly disassembles nucleosomes to regulate DNA repair www.cell.com/molecular-ce...
Ashish Verma/Changlei Zhu and Hai T. Dao (St. Jude Children's Research Hospital) in Molecular Cell
#Epigenetics #PPAR1 #Nucleosomes #DNARepair
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epigenometech.com
Ashish Verma/Changlei Zhu and Hai T. Dao (St. Jude Children's Research Hospital) in Molecular Cell
#Epigenetics #PPAR1 #Nucleosomes #DNARepair
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epigenometech.com
October 30, 2026 at 8:02 AM
Epigenetics Update - PARP1 directly disassembles nucleosomes to regulate DNA repair www.cell.com/molecular-ce...
Ashish Verma/Changlei Zhu and Hai T. Dao (St. Jude Children's Research Hospital) in Molecular Cell
#Epigenetics #PPAR1 #Nucleosomes #DNARepair
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epigenometech.com
Ashish Verma/Changlei Zhu and Hai T. Dao (St. Jude Children's Research Hospital) in Molecular Cell
#Epigenetics #PPAR1 #Nucleosomes #DNARepair
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epigenometech.com
Preprint: nucleosomes with active (H3K4me2) and repressive (H3K9me3) marks sit at distinct positions in plasma cfDNA. 50 of these sites read tumor fraction and neuroendocrine transformation from routine sequencing.
doi.org/10.64898/202...
@gungulati.bsky.social
doi.org/10.64898/202...
@gungulati.bsky.social
October 29, 2026 at 5:15 PM
Preprint: nucleosomes with active (H3K4me2) and repressive (H3K9me3) marks sit at distinct positions in plasma cfDNA. 50 of these sites read tumor fraction and neuroendocrine transformation from routine sequencing.
doi.org/10.64898/202...
@gungulati.bsky.social
doi.org/10.64898/202...
@gungulati.bsky.social
Different transcription systems shape chromatin behavior in distinct nuclear regions
phys.org/news/2026-10...
phys.org/news/2026-10...
Different transcription systems shape chromatin behavior in distinct nuclear regions
Inside the cell nucleus, DNA is wrapped around histone proteins to form nucleosomes, which are further organized into chromatin. Chromatin has traditionally been divided into transcriptionally active ...
phys.org
November 2, 2026 at 8:38 PM
Different transcription systems shape chromatin behavior in distinct nuclear regions
phys.org/news/2026-10...
phys.org/news/2026-10...
Blocking Pol II increased chromatin movement in several chromatin classes. Blocking Pol I selectively increased movement in one heterochromatin population around nucleoli. doi.org/hcqthq
Different transcription systems shape chromatin behavior in distinct nuclear regions
Inside the cell nucleus, DNA is wrapped around histone proteins to form nucleosomes, which are further organized into chromatin.
phys.org
October 30, 2026 at 5:00 PM
Blocking Pol II increased chromatin movement in several chromatin classes. Blocking Pol I selectively increased movement in one heterochromatin population around nucleoli. doi.org/hcqthq
Mariani et al. in the Bulyk Lab at Brigham and Women's Hospital show that H3K27ac, H3K27me3 and H3K9me3 directly reshape how seven human TFs bind nucleosomes, shifting FOXA1 and PAX7 monomer-dimer preferences, with H3K27ac blocking internal sites to favor end-binding.
bioRxiv (Evolutionary Biology)
www.biorxiv.org
October 29, 2026 at 12:22 AM
Mariani et al. in the Bulyk Lab at Brigham and Women's Hospital show that H3K27ac, H3K27me3 and H3K9me3 directly reshape how seven human TFs bind nucleosomes, shifting FOXA1 and PAX7 monomer-dimer preferences, with H3K27ac blocking internal sites to favor end-binding.
Chromatin regulators move nucleosomes in 10 bp steps that preserve rotational setting, using the shallow energy potential that DNA sequence creates.
www.cell.com/cell-reports... 🧵
www.cell.com/cell-reports... 🧵
Rotational settings quantize nucleosome movement by chromatin regulators
Chromatin regulators are thought to override intrinsic DNA sequence preferences. La
et al. show regulators move nucleosomes in quantized 10 bp steps to take advantage
of the shallow energy potential c...
www.cell.com
October 23, 2026 at 10:30 PM
Chromatin regulators move nucleosomes in 10 bp steps that preserve rotational setting, using the shallow energy potential that DNA sequence creates.
www.cell.com/cell-reports... 🧵
www.cell.com/cell-reports... 🧵
The authors included RRIDs in their in Molecular Cell paper! Thanks for making your methods matter! #ReproducibleResearch #reproducibility #OpenScience
PARP1 directly disassembles nucleosomes to regulate DNA repair
Read the full paper: PARP1 directly disassembles nucleosomes to regulate DNA repair
doi.org
October 25, 2026 at 7:00 AM
The authors included RRIDs in their in Molecular Cell paper! Thanks for making your methods matter! #ReproducibleResearch #reproducibility #OpenScience
Online Now: PARP1 directly disassembles nucleosomes to regulate DNA repair Online now:
PARP1 directly disassembles nucleosomes to regulate DNA repair
Verma, Zhu, et al. show that PARP1 promotes NAD+-dependent eviction of H2A-H2B dimers, generating oriented hexasomes with increased DNA accessibility. The H2A C-terminal tail supports this remodeling activity, linking PARP1-dependent nucleosome disassembly to cellular responses to DNA damage.
dlvr.it
October 23, 2026 at 7:14 PM
Online Now: PARP1 directly disassembles nucleosomes to regulate DNA repair Online now:
We built a multitaper framework to score rotational setting at individual nucleosomes in yeast and mouse cells, including from standard MNase-seq.
October 23, 2026 at 10:30 PM
We built a multitaper framework to score rotational setting at individual nucleosomes in yeast and mouse cells, including from standard MNase-seq.
🧬PARP1 isn't just a DNA damage sensor. It actively evicts histone proteins H2A-H2B dimers to transform dense nucleosomes into open hexasomes to regulate DNA repair. This motor-free remodeling links basic chromatin biology to targeted cancer therapies
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
www.sciencedirect.com
October 24, 2026 at 5:42 PM
🧬PARP1 isn't just a DNA damage sensor. It actively evicts histone proteins H2A-H2B dimers to transform dense nucleosomes into open hexasomes to regulate DNA repair. This motor-free remodeling links basic chromatin biology to targeted cancer therapies
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
RRID:AB_2615081 from @activemotifusa.bsky.social was used by authors in their Cell Reports Methods paper. We value the author's support of reproducibility. #accelerateopenscience #BetterScience #STMpublishing
mChIP-seq for multiplex epigenomic profiling uncovers cancer-specific histone features in cellular and circulating nucleosomes
Read the full paper: mChIP-seq for multiplex epigenomic profiling uncovers cancer-specific histone features in cellular and circulating nucleosomes
doi.org
October 24, 2026 at 7:00 AM
RRID:AB_2615081 from @activemotifusa.bsky.social was used by authors in their Cell Reports Methods paper. We value the author's support of reproducibility. #accelerateopenscience #BetterScience #STMpublishing
Scientists identified a direct role for PARP1 in chromatin remodeling, revealing for the first time how the protein partially disassembles nucleosomes to make damaged DNA accessible for repair.
🧪 Read how this mechanism could inform targeted cancer therapies: https://bit.ly/4zRmFDb
🧪 Read how this mechanism could inform targeted cancer therapies: https://bit.ly/4zRmFDb
bit.ly
October 23, 2026 at 4:00 PM
Scientists identified a direct role for PARP1 in chromatin remodeling, revealing for the first time how the protein partially disassembles nucleosomes to make damaged DNA accessible for repair.
🧪 Read how this mechanism could inform targeted cancer therapies: https://bit.ly/4zRmFDb
🧪 Read how this mechanism could inform targeted cancer therapies: https://bit.ly/4zRmFDb
Histone modifications directly modulate transcription factor binding to nucleosomes https://www.biorxiv.org/content/10.64898/2026.10.21.760833v1
October 23, 2026 at 10:16 AM
Histone modifications directly modulate transcription factor binding to nucleosomes https://www.biorxiv.org/content/10.64898/2026.10.21.760833v1
Histone modifications directly modulate transcription factor binding to nucleosomes https://www.biorxiv.org/content/10.64898/2026.10.21.760833v1
October 23, 2026 at 10:16 AM
Histone modifications directly modulate transcription factor binding to nucleosomes https://www.biorxiv.org/content/10.64898/2026.10.21.760833v1
mChIP-seq for multiplex epigenomic profiling uncovers cancer-specific histone features in cellular and circulating nucleosomes
mChIP-seq for multiplex epigenomic profiling uncovers cancer-specific histone features in cellular and circulating nucleosomes
Sun et al. develop mChIP-seq, an epigenomic profiling technology that enables multiplexed and multifactorial ChIP for both cellular and cell-free samples. They demonstrate the technology by profiling cancer-specific features of histone marks in cancer cells and in plasma cell-free nucleosomes from breast cancer patients.
dlvr.it
October 22, 2026 at 6:49 PM
mChIP-seq for multiplex epigenomic profiling uncovers cancer-specific histone features in cellular and circulating nucleosomes
Epigenetics Update - Molecular mechanism of residue-specific nucleosome acetylation www.cell.com/molecular-ce...
Marisa Müller (lmu.de) and Sebastian Eustermann (embl.org) in Molecular Cell
#Epigenetics #Nucleosomes #Chromatin #H4K16
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Discover the breakthrough at epigenometech.com
Marisa Müller (lmu.de) and Sebastian Eustermann (embl.org) in Molecular Cell
#Epigenetics #Nucleosomes #Chromatin #H4K16
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Discover the breakthrough at epigenometech.com
October 21, 2026 at 6:43 AM
Epigenetics Update - Molecular mechanism of residue-specific nucleosome acetylation www.cell.com/molecular-ce...
Marisa Müller (lmu.de) and Sebastian Eustermann (embl.org) in Molecular Cell
#Epigenetics #Nucleosomes #Chromatin #H4K16
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Discover the breakthrough at epigenometech.com
Marisa Müller (lmu.de) and Sebastian Eustermann (embl.org) in Molecular Cell
#Epigenetics #Nucleosomes #Chromatin #H4K16
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Discover the breakthrough at epigenometech.com
RRIDs were included in this in Nature Communications paper. Thanks for making your methods matter! #STMpublishing #BetterScience #ReproducibleResearch
Structural Characterization of Native RNA Polymerase II Transcription Complexes and Nucleosomes in Drosophila melanogaster - Nature Communications
Here the authors show that native Pol II transcription complexes are isolated directly from D. melanogaster embryos by using a robust one-step affinity purification and by using cryo-EM resolve multiple functional states of the Pol II transcription complex and nucleosome.
doi.org
October 23, 2026 at 7:01 AM
RRIDs were included in this in Nature Communications paper. Thanks for making your methods matter! #STMpublishing #BetterScience #ReproducibleResearch
Extrachromosomal circular DNA formation follows a 10.5-bp periodicity reflecting the minor groove orientation around nucleosomes https://www.biorxiv.org/content/10.64898/2026.10.19.760212v1
October 21, 2026 at 12:32 PM
Extrachromosomal circular DNA formation follows a 10.5-bp periodicity reflecting the minor groove orientation around nucleosomes https://www.biorxiv.org/content/10.64898/2026.10.19.760212v1
Extrachromosomal circular DNA formation follows a 10.5-bp periodicity reflecting the minor groove orientation around nucleosomes https://www.biorxiv.org/content/10.64898/2026.10.19.760212v1
October 21, 2026 at 12:32 PM
Extrachromosomal circular DNA formation follows a 10.5-bp periodicity reflecting the minor groove orientation around nucleosomes https://www.biorxiv.org/content/10.64898/2026.10.19.760212v1