6. Even though it's RT-LAMP instead of PCR, that doesn't stop me from getting the PCR song stuck in my head anyway m.youtube.com/watch?v=x5yP...
The PCR Song
YouTube video by CompareNetworks Production Group
m.youtube.com
December 23, 2024 at 2:11 AM
6. Even though it's RT-LAMP instead of PCR, that doesn't stop me from getting the PCR song stuck in my head anyway m.youtube.com/watch?v=x5yP...
It is Day Five after travel and the tiny PCR machine that sits on my kitchen table tells me I managed a cross country flight, two 11-hour drives, another flight, getting stuck in Vegas overnight, and another flight without catching COVID
April 30, 2025 at 5:06 PM
It is Day Five after travel and the tiny PCR machine that sits on my kitchen table tells me I managed a cross country flight, two 11-hour drives, another flight, getting stuck in Vegas overnight, and another flight without catching COVID
normal person: this antivaxxer and measles denialist is insane
me: actually they don’t swab the rash, they swab the nose or throat and collect blood
www.cdc.gov/measles/php/...
me: actually they don’t swab the rash, they swab the nose or throat and collect blood
www.cdc.gov/measles/php/...
September 28, 2026 at 12:28 AM
normal person: this antivaxxer and measles denialist is insane
me: actually they don’t swab the rash, they swab the nose or throat and collect blood
www.cdc.gov/measles/php/...
me: actually they don’t swab the rash, they swab the nose or throat and collect blood
www.cdc.gov/measles/php/...
I was submerged in work on the current hantavirus outbreak in the recent week and reminded myself how important social media can be during outbreaks!
The team at our Centre did quite some work, diagnosing a case in Switzerland, identifying the Andes strain by RT-PCR & full genome released by friday
The team at our Centre did quite some work, diagnosing a case in Switzerland, identifying the Andes strain by RT-PCR & full genome released by friday
May 11, 2026 at 8:37 PM
I was submerged in work on the current hantavirus outbreak in the recent week and reminded myself how important social media can be during outbreaks!
The team at our Centre did quite some work, diagnosing a case in Switzerland, identifying the Andes strain by RT-PCR & full genome released by friday
The team at our Centre did quite some work, diagnosing a case in Switzerland, identifying the Andes strain by RT-PCR & full genome released by friday
My friend got me a free RT-PCR from a company that was giving away old equipment. It's a good day.
November 14, 2025 at 5:13 PM
My friend got me a free RT-PCR from a company that was giving away old equipment. It's a good day.
Familiar. When DNA Nudge were awarded the contract for RT-PCR testing during Covid, I have first hand accounts that hospitals abandoned them after days because they didn’t work, overheated, ran out of reagents and gave false positives.
🔴 Remember the £330million contract given to Trump donor Peter Thiel's firm Palantir to build an NHS data platform?
Well we've found out that most English hospitals aren't using it - because it's not very good
Scoop on Democracy for Sale
democracyforsale.substack.com/p/palantirs-...
Well we've found out that most English hospitals aren't using it - because it's not very good
Scoop on Democracy for Sale
democracyforsale.substack.com/p/palantirs-...
Palantir's NHS data platform rejected by most hospitals
US spy tech firm's £330million software isn't much cop, experts say
democracyforsale.substack.com
May 15, 2025 at 6:51 AM
Familiar. When DNA Nudge were awarded the contract for RT-PCR testing during Covid, I have first hand accounts that hospitals abandoned them after days because they didn’t work, overheated, ran out of reagents and gave false positives.
May 22, 2026 at 11:40 AM
(2/2) Key takeaways:
• Vaccinated people had milder symptoms, but breakthrough cases still occurred
• Urine RT-PCR improved measles detection, especially in vaccinated patients
• Ensure 2-dose MMR coverage for travelers
#Measles #MMWR #VaccinesWork #TravelHealth
Read: www.cdc.gov/mmwr/volumes...
• Vaccinated people had milder symptoms, but breakthrough cases still occurred
• Urine RT-PCR improved measles detection, especially in vaccinated patients
• Ensure 2-dose MMR coverage for travelers
#Measles #MMWR #VaccinesWork #TravelHealth
Read: www.cdc.gov/mmwr/volumes...
January 30, 2026 at 1:50 AM
(2/2) Key takeaways:
• Vaccinated people had milder symptoms, but breakthrough cases still occurred
• Urine RT-PCR improved measles detection, especially in vaccinated patients
• Ensure 2-dose MMR coverage for travelers
#Measles #MMWR #VaccinesWork #TravelHealth
Read: www.cdc.gov/mmwr/volumes...
• Vaccinated people had milder symptoms, but breakthrough cases still occurred
• Urine RT-PCR improved measles detection, especially in vaccinated patients
• Ensure 2-dose MMR coverage for travelers
#Measles #MMWR #VaccinesWork #TravelHealth
Read: www.cdc.gov/mmwr/volumes...
The "tabletop PCR" is really more accurately described as "tabletop RT-LAMP", but my forensic-DNA-analyst wife says RT-LAMP is so a form of PCR, heh. The one we use is the Pluslife (unofficial FAQ: virus.sucks )
virus.sucks
virus.sucks
November 21, 2025 at 8:35 PM
The "tabletop PCR" is really more accurately described as "tabletop RT-LAMP", but my forensic-DNA-analyst wife says RT-LAMP is so a form of PCR, heh. The one we use is the Pluslife (unofficial FAQ: virus.sucks )
Fraunhofer IMM scientists built a pocket PCR device that detects SARS-CoV-2 in 15 minutes.
It runs 40 PCR cycles faster than a lab thermocycler and works with saliva, showing lab-level sensitivity in a portable chip system.
www.researchsquare.com/article/rs-7...
It runs 40 PCR cycles faster than a lab thermocycler and works with saliva, showing lab-level sensitivity in a portable chip system.
www.researchsquare.com/article/rs-7...
SARS-CoV-2 detection via high-sensitivity RT-PCR in 15 minutes using microfluidic COP chips
The Moving-Plug PCR System, developed by Fraunhofer IMM, is a portable device that dramatically accelerates PCR reaction speeds by eliminating the time-consuming heating and cooling cycles traditional...
www.researchsquare.com
September 15, 2025 at 3:54 PM
Fraunhofer IMM scientists built a pocket PCR device that detects SARS-CoV-2 in 15 minutes.
It runs 40 PCR cycles faster than a lab thermocycler and works with saliva, showing lab-level sensitivity in a portable chip system.
www.researchsquare.com/article/rs-7...
It runs 40 PCR cycles faster than a lab thermocycler and works with saliva, showing lab-level sensitivity in a portable chip system.
www.researchsquare.com/article/rs-7...
It was mostly confined to one hospital system might, and it is the only variant ever to have a very clear BAL mutation pattern. So seems likely these muts increase severity in both mice & humans, likely by adapting to the deep lung. 4/4
www.clinicalmicrobiologyandinfection.org/article/S119...
www.clinicalmicrobiologyandinfection.org/article/S119...
A new SARS-CoV-2 variant with high lethality poorly detected by RT-PCR on nasopharyngeal samples: an observational study
In early January 2021 an outbreak of nosocomial cases of coronavirus disease 2019
(COVID-19) emerged in Western France; RT-PCR tests were repeatedly negative on nasopharyngeal
samples but positive on ...
www.clinicalmicrobiologyandinfection.org
January 10, 2026 at 11:08 PM
It was mostly confined to one hospital system might, and it is the only variant ever to have a very clear BAL mutation pattern. So seems likely these muts increase severity in both mice & humans, likely by adapting to the deep lung. 4/4
www.clinicalmicrobiologyandinfection.org/article/S119...
www.clinicalmicrobiologyandinfection.org/article/S119...
January 23, 2020: Corman et al., Eurosurveillance (assay development) designed the first real-time RT-PCR test for the novel coronavirus (2019-nCoV) using publicly released genome sequences
December 21, 2025 at 5:33 AM
January 23, 2020: Corman et al., Eurosurveillance (assay development) designed the first real-time RT-PCR test for the novel coronavirus (2019-nCoV) using publicly released genome sequences
Madrid researchers tested 120 positive swabs and found SARS-CoV-2 RNA can persist for months.
RT-PCR results and Ct values fluctuated, showing why clinical decisions should not rely on repeated PCR tests alone.
journals.asm.org/doi/10.1128/...
RT-PCR results and Ct values fluctuated, showing why clinical decisions should not rely on repeated PCR tests alone.
journals.asm.org/doi/10.1128/...
Prolonged SARS-CoV-2 RNA detection by RT-PCR: variability in cycle threshold values over time | Microbiology Spectrum
This study investigates the prolonged persistence of SARS-CoV-2 detected by reverse
transcription-PCR (RT-PCR) at human body temperature and the evolution of cycle threshold
(Ct) values in positive re...
journals.asm.org
September 8, 2025 at 5:45 PM
Madrid researchers tested 120 positive swabs and found SARS-CoV-2 RNA can persist for months.
RT-PCR results and Ct values fluctuated, showing why clinical decisions should not rely on repeated PCR tests alone.
journals.asm.org/doi/10.1128/...
RT-PCR results and Ct values fluctuated, showing why clinical decisions should not rely on repeated PCR tests alone.
journals.asm.org/doi/10.1128/...
Oh bah, une autre bonne nouvelle, tadaaa enfin le résultat PCR et c'est un grand NON qui soulage !
C'était la première fois que je faisais un repas en intérieur (pour mon anniv, ha ha ha) sans purificateur ni masque dans la voiture familiale parce que ça tendait trop l'ambiance, paye ton stress !
C'était la première fois que je faisais un repas en intérieur (pour mon anniv, ha ha ha) sans purificateur ni masque dans la voiture familiale parce que ça tendait trop l'ambiance, paye ton stress !
March 26, 2025 at 12:59 PM
Oh bah, une autre bonne nouvelle, tadaaa enfin le résultat PCR et c'est un grand NON qui soulage !
C'était la première fois que je faisais un repas en intérieur (pour mon anniv, ha ha ha) sans purificateur ni masque dans la voiture familiale parce que ça tendait trop l'ambiance, paye ton stress !
C'était la première fois que je faisais un repas en intérieur (pour mon anniv, ha ha ha) sans purificateur ni masque dans la voiture familiale parce que ça tendait trop l'ambiance, paye ton stress !
A pervasive RT–qPCR artifact inflates RNA knockdown by RNA-targeting CRISPR -
Yikey Yikes! BTW, similar concerns apply to siRNAs/ASOs that are sometimes suspected to affect RT and/or PCR . So nice somebody looked into this! rdcu.be/RUYFaGa649va
Yikey Yikes! BTW, similar concerns apply to siRNAs/ASOs that are sometimes suspected to affect RT and/or PCR . So nice somebody looked into this! rdcu.be/RUYFaGa649va
rdcu.be
September 1, 2026 at 12:11 PM
A pervasive RT–qPCR artifact inflates RNA knockdown by RNA-targeting CRISPR -
Yikey Yikes! BTW, similar concerns apply to siRNAs/ASOs that are sometimes suspected to affect RT and/or PCR . So nice somebody looked into this! rdcu.be/RUYFaGa649va
Yikey Yikes! BTW, similar concerns apply to siRNAs/ASOs that are sometimes suspected to affect RT and/or PCR . So nice somebody looked into this! rdcu.be/RUYFaGa649va
High-risk patients with COVID symptoms should use PCR rather than rapid tests, study suggests.
"Study findings that reveal a sensitivity of 47% for COVID-19 rapid antigen tests (RATs) compared with reverse-transcription polymerase chain reaction (RT-PCR) tests during Omicron variant predominance"
"Study findings that reveal a sensitivity of 47% for COVID-19 rapid antigen tests (RATs) compared with reverse-transcription polymerase chain reaction (RT-PCR) tests during Omicron variant predominance"
High-risk patients with COVID symptoms should use PCR rather than rapid tests, study suggests
www.cidrap.umn.edu
May 2, 2024 at 11:52 PM
High-risk patients with COVID symptoms should use PCR rather than rapid tests, study suggests.
"Study findings that reveal a sensitivity of 47% for COVID-19 rapid antigen tests (RATs) compared with reverse-transcription polymerase chain reaction (RT-PCR) tests during Omicron variant predominance"
"Study findings that reveal a sensitivity of 47% for COVID-19 rapid antigen tests (RATs) compared with reverse-transcription polymerase chain reaction (RT-PCR) tests during Omicron variant predominance"
Hola señora! ¿Tiene un minuto para hablarle de los distintos tipos de PCR y por qué RT-PCR no significa PCR a tiempo real? 😌
The Book Of Mormon Missionary Wave
ALT: The Book Of Mormon Missionary Wave
static.klipy.com
September 10, 2026 at 5:48 AM
Hola señora! ¿Tiene un minuto para hablarle de los distintos tipos de PCR y por qué RT-PCR no significa PCR a tiempo real? 😌
Eu trabalho no SUS , na área de apoio ao diagnóstico, e vou dar só um exemplo na época da PANDEMIA: UM TESTE DE RT PCR NOS EUA chegou a custar 8 MIL DÓLARES!!! Simmm hoje seria quase 48.000,00 reais. Quem diabos nesse país , pegando da classe média para baixo ia testar? VAI SE LASCAR @arminioFRAGA
October 12, 2024 at 5:44 PM
Eu trabalho no SUS , na área de apoio ao diagnóstico, e vou dar só um exemplo na época da PANDEMIA: UM TESTE DE RT PCR NOS EUA chegou a custar 8 MIL DÓLARES!!! Simmm hoje seria quase 48.000,00 reais. Quem diabos nesse país , pegando da classe média para baixo ia testar? VAI SE LASCAR @arminioFRAGA
Hace cinco años que nos confinaron en casa por una pandemia causada por un nuevo virus que podíamos detectar mediante #RTPCR, técnica que pocos entendían. Y decidí explicarla… con piezas de #TENTE … y grabé un primer video para #YouTube… y se viralizó!
youtu.be/CxSu61UcQXk
youtu.be/CxSu61UcQXk
¿Cómo diagnosticar coronavirus con la técnica RT-PCR?
YouTube video by Lluis Montoliu
youtu.be
March 14, 2026 at 8:22 AM
Hace cinco años que nos confinaron en casa por una pandemia causada por un nuevo virus que podíamos detectar mediante #RTPCR, técnica que pocos entendían. Y decidí explicarla… con piezas de #TENTE … y grabé un primer video para #YouTube… y se viralizó!
youtu.be/CxSu61UcQXk
youtu.be/CxSu61UcQXk
Guidance Summary
For Healthcare Providers:
• Test for Dengue Early: Use the CDC DENV-1-4 real-time RT-PCR assay when dengue is suspected.
• Consider Dengue in Diagnosis: Be vigilant in patients with fever and recent travel to affected regions or local transmission areas.
For Healthcare Providers:
• Test for Dengue Early: Use the CDC DENV-1-4 real-time RT-PCR assay when dengue is suspected.
• Consider Dengue in Diagnosis: Be vigilant in patients with fever and recent travel to affected regions or local transmission areas.
March 18, 2025 at 5:49 PM
Guidance Summary
For Healthcare Providers:
• Test for Dengue Early: Use the CDC DENV-1-4 real-time RT-PCR assay when dengue is suspected.
• Consider Dengue in Diagnosis: Be vigilant in patients with fever and recent travel to affected regions or local transmission areas.
For Healthcare Providers:
• Test for Dengue Early: Use the CDC DENV-1-4 real-time RT-PCR assay when dengue is suspected.
• Consider Dengue in Diagnosis: Be vigilant in patients with fever and recent travel to affected regions or local transmission areas.
I can't wait to be able to run RT-qPCR in my lab!!!
Eagerly awaiting my Pocket qPCR to arrive. I can finally use my 50k units of reverse transcriptase I got from Gene and Cell Technologies a few years ago.
gaudi.ch/PocketPCR/in...
Eagerly awaiting my Pocket qPCR to arrive. I can finally use my 50k units of reverse transcriptase I got from Gene and Cell Technologies a few years ago.
gaudi.ch/PocketPCR/in...
qPocketPCR – The Ultra-Compact Real-Time PCR Thermocycler – PocketPCR
gaudi.ch
November 29, 2025 at 9:55 AM
I can't wait to be able to run RT-qPCR in my lab!!!
Eagerly awaiting my Pocket qPCR to arrive. I can finally use my 50k units of reverse transcriptase I got from Gene and Cell Technologies a few years ago.
gaudi.ch/PocketPCR/in...
Eagerly awaiting my Pocket qPCR to arrive. I can finally use my 50k units of reverse transcriptase I got from Gene and Cell Technologies a few years ago.
gaudi.ch/PocketPCR/in...
Antihistaminika, insbesondere Histaminrezeptor-H1 (HRH1)-Antagonisten, hemmen wirksam die #SARSCoV2-Infektion
In dieser Studie wurde „nachgewiesen, dass HRH1 als alternativer Rezeptor für #SARSCoV2 fungiert, indem er direkt an das virale Spike-Protein bindet.🧵
#COVID19 #Corona
In dieser Studie wurde „nachgewiesen, dass HRH1 als alternativer Rezeptor für #SARSCoV2 fungiert, indem er direkt an das virale Spike-Protein bindet.🧵
#COVID19 #Corona
July 6, 2024 at 1:11 PM
Antihistaminika, insbesondere Histaminrezeptor-H1 (HRH1)-Antagonisten, hemmen wirksam die #SARSCoV2-Infektion
In dieser Studie wurde „nachgewiesen, dass HRH1 als alternativer Rezeptor für #SARSCoV2 fungiert, indem er direkt an das virale Spike-Protein bindet.🧵
#COVID19 #Corona
In dieser Studie wurde „nachgewiesen, dass HRH1 als alternativer Rezeptor für #SARSCoV2 fungiert, indem er direkt an das virale Spike-Protein bindet.🧵
#COVID19 #Corona