𝗦𝗣-𝗦𝗧𝗢𝗥𝗠 (𝗦𝗽𝗲𝗰𝘁𝗿𝗮𝗹-𝗣𝗵𝗮𝘀𝗼𝗿 𝗦𝗧𝗢𝗥𝗠) 𝘗𝘳𝘦𝘗𝘳𝘪𝘯𝘵 𝘸𝘰𝘳𝘵𝘩 𝘢 𝘭𝘰𝘰𝘬 “𝗛𝗶𝗴𝗵 𝗧𝗵𝗿𝗼𝘂𝗴𝗵𝗽𝘂𝘁 𝗛𝘆𝗽𝗲𝗿𝘀𝗽𝗲𝗰𝘁𝗿𝗮𝗹 𝗮𝗻𝗱 𝗠𝘂𝗹𝘁𝗶𝗽𝗹𝗲𝘅𝗲𝗱 𝗦𝘂𝗽𝗲𝗿-𝗥𝗲𝘀𝗼𝗹𝘂𝘁𝗶𝗼𝗻 𝗙𝗹𝘂𝗼𝗿𝗲𝘀𝗰𝗲𝗻𝗰𝗲 𝗜𝗺𝗮𝗴𝗶𝗻𝗴 𝗯𝘆 𝗦𝗣-𝗦𝗧𝗢𝗥𝗠” (𝘗𝘰𝘵𝘵 𝘦𝘵 𝘢𝘭., 𝘣𝘪𝘰𝘙𝘹𝘪𝘷, 𝘚𝘦𝘱𝘵 2025 — https://lnkd.in/d5smSTUN ) A clever implementation of spectral-phasor analysis directly in hardware — achieving simultaneous five-color super-resolution imaging within about a minute. By merging sine/cosine filter optics with dSTORM principles, the method reduces spectral crosstalk (<5 %) and removes the need for sequential channel switching. The hardware-level multiplexing is a decisive step toward practical multi-target SMLM, though the current proof remains 2D and relies on custom filter sets. 𝘈 𝘵𝘢𝘯𝘨𝘪𝘣𝘭𝘦 𝘢𝘥𝘷𝘢𝘯𝘤𝘦 𝘪𝘯 𝘮𝘶𝘭𝘵𝘪𝘱𝘭𝘦𝘹𝘦𝘥 𝘱𝘩𝘦𝘯𝘰𝘵𝘺𝘱𝘪𝘯𝘨 — 𝘰𝘯𝘦 𝘵𝘩𝘢𝘵 𝘤𝘰𝘶𝘭𝘥 𝘳𝘦𝘴𝘩𝘢𝘱𝘦 𝘩𝘰𝘸 𝘴𝘱𝘦𝘤𝘵𝘳𝘢𝘭 𝘪𝘯𝘧𝘰𝘳𝘮𝘢𝘵𝘪𝘰𝘯 𝘪𝘴 𝘪𝘯𝘵𝘦𝘨𝘳𝘢𝘵𝘦𝘥 𝘪𝘯𝘵𝘰 𝘏𝘊𝘐 𝘸𝘰𝘳𝘬𝘧𝘭𝘰𝘸𝘴 𝘰𝘯𝘤𝘦 𝘴𝘤𝘢𝘭𝘦𝘥 𝘵𝘰 𝟥𝘋 𝘢𝘯𝘥 𝘭𝘪𝘷𝘦 𝘪𝘮𝘢𝘨𝘪𝘯𝘨. #SPSTORM #SpectralImaging #SMLM #Multiplex #HighContent #FluorescenceMicroscopy #bioRxiv