#flourescent(almost)friday
(make sure to full screen this!) Some hippocampal neurons and dendritic arbors visualized from mCherry with a 3.6x objective in a SmartSPIM using iDisco.
(make sure to full screen this!) Some hippocampal neurons and dendritic arbors visualized from mCherry with a 3.6x objective in a SmartSPIM using iDisco.
December 5, 2024 at 6:50 PM
#flourescent(almost)friday
(make sure to full screen this!) Some hippocampal neurons and dendritic arbors visualized from mCherry with a 3.6x objective in a SmartSPIM using iDisco.
(make sure to full screen this!) Some hippocampal neurons and dendritic arbors visualized from mCherry with a 3.6x objective in a SmartSPIM using iDisco.
Lightsheet microscopy is unparalleled in its ability to resolve whole circuits in the mouse brain. Michelle Bedebaugh let me image one of her Rabies MC3R Cre samples with the 15X objective on my smartSPIM and we are very happy with the results. 🧪
December 4, 2024 at 2:02 AM
Lightsheet microscopy is unparalleled in its ability to resolve whole circuits in the mouse brain. Michelle Bedebaugh let me image one of her Rabies MC3R Cre samples with the 15X objective on my smartSPIM and we are very happy with the results. 🧪
Studying neural circuits is all about connectivity. So why cut up your sample? This #FluorescenceFriday, we're highlighting some mesmerizing 3D neural circuit data scientists have generated with our sample processing techniques and #SmartSPIM imaging on whole mouse brains.
March 6, 2026 at 2:48 PM
Studying neural circuits is all about connectivity. So why cut up your sample? This #FluorescenceFriday, we're highlighting some mesmerizing 3D neural circuit data scientists have generated with our sample processing techniques and #SmartSPIM imaging on whole mouse brains.
How does the cerebral cortex form connections with other parts of the CNS? This #SmartSPIM data again comes from @shreyasms.bsky.social, this time showing projections of ET neurons from the visual cortex to different parts of the 🧠. Read the paper to learn more: bit.ly/48YsZxn #FluorescenceFriday
May 8, 2026 at 3:57 PM
How does the cerebral cortex form connections with other parts of the CNS? This #SmartSPIM data again comes from @shreyasms.bsky.social, this time showing projections of ET neurons from the visual cortex to different parts of the 🧠. Read the paper to learn more: bit.ly/48YsZxn #FluorescenceFriday
Up in Acadia at the @mdibiolab.bsky.social for #LSFM25! Here's a cleared whole mouse head on the SmartSPIM, 3.6X objective for #3DThursday.
🔎 @jtakatoh.bsky.social, now at @stonybrooku.bsky.social
🔬 LifeCanvas Technologies, lifecanvastech.com/products/sma...
🥽 syGlass.io
@lsfm-na.bsky.social
🔎 @jtakatoh.bsky.social, now at @stonybrooku.bsky.social
🔬 LifeCanvas Technologies, lifecanvastech.com/products/sma...
🥽 syGlass.io
@lsfm-na.bsky.social
June 5, 2025 at 12:54 PM
Up in Acadia at the @mdibiolab.bsky.social for #LSFM25! Here's a cleared whole mouse head on the SmartSPIM, 3.6X objective for #3DThursday.
🔎 @jtakatoh.bsky.social, now at @stonybrooku.bsky.social
🔬 LifeCanvas Technologies, lifecanvastech.com/products/sma...
🥽 syGlass.io
@lsfm-na.bsky.social
🔎 @jtakatoh.bsky.social, now at @stonybrooku.bsky.social
🔬 LifeCanvas Technologies, lifecanvastech.com/products/sma...
🥽 syGlass.io
@lsfm-na.bsky.social
Somatostatin-expressing (SST) neurons are a subpopulation of highly heterogeneous GABAergic interneurons that play a variety of functions in the brain. This #FluorescenceFriday video shows SST labeling in a whole mouse brain, labeled with SmartBatch+ and imaged with #SmartSPIM.
March 13, 2026 at 1:30 PM
Somatostatin-expressing (SST) neurons are a subpopulation of highly heterogeneous GABAergic interneurons that play a variety of functions in the brain. This #FluorescenceFriday video shows SST labeling in a whole mouse brain, labeled with SmartBatch+ and imaged with #SmartSPIM.
How does the brain control limb movement? In a new paper, Julia Kaiser et al. investigate the development of different populations of neurons that project to the brain stem and spinal cord, seen in this SmartSPIM video. Read the open access study: bit.ly/4y5YIH9 #FluorescenceFriday
August 28, 2026 at 1:36 PM
How does the brain control limb movement? In a new paper, Julia Kaiser et al. investigate the development of different populations of neurons that project to the brain stem and spinal cord, seen in this SmartSPIM video. Read the open access study: bit.ly/4y5YIH9 #FluorescenceFriday
First data set collected at #Sample2Image #EMBO by @lifecanvastech.bsky.social SmartSPIM team rocking Oeiras PT
July 5, 2026 at 10:38 AM
First data set collected at #Sample2Image #EMBO by @lifecanvastech.bsky.social SmartSPIM team rocking Oeiras PT
#SmartSPIM light sheet microscopy is coming to Barcelona! We can't wait to show you how easy it is to capture hi-res 3D data throughout your entire sample, like this intact mouse brain and spinal cord, mouse duodenum, and rat heart. #FluorescenceFriday @spaommeeting.bsky.social
September 25, 2026 at 1:48 PM
#SmartSPIM light sheet microscopy is coming to Barcelona! We can't wait to show you how easy it is to capture hi-res 3D data throughout your entire sample, like this intact mouse brain and spinal cord, mouse duodenum, and rat heart. #FluorescenceFriday @spaommeeting.bsky.social
Incredibly exciting to see our tissue clearing and #SmartSPIM imaging tech in some of these groundbreaking studies!
The developing brain? Yeah, we have it covered.
The first brain cell atlas of the developing mammalian brain is now available to curious minds and researchers everywhere.
Check out the findings in @nature.com: www.nature.com/collections/...
🎨 Cover art by Jasiek Krzysztofiak
The first brain cell atlas of the developing mammalian brain is now available to curious minds and researchers everywhere.
Check out the findings in @nature.com: www.nature.com/collections/...
🎨 Cover art by Jasiek Krzysztofiak
November 7, 2025 at 9:51 PM
Incredibly exciting to see our tissue clearing and #SmartSPIM imaging tech in some of these groundbreaking studies!
@brandeisuniversity.bsky.social has a new lightsheet for #MicroscopyMonday !💡New England microscopy cores - do you have a system you want advertised? Send us an image! #NESM
🧠: TdTomato Mouse Brain
🔬: #LifeCanvas SmartSPIM, 1.6x obj
🥼: @andystoneimaging.bsky.social
🏛️: Brandeis LMCF (RRID:SCR_02589)
🧠: TdTomato Mouse Brain
🔬: #LifeCanvas SmartSPIM, 1.6x obj
🥼: @andystoneimaging.bsky.social
🏛️: Brandeis LMCF (RRID:SCR_02589)
July 14, 2025 at 6:00 PM
@brandeisuniversity.bsky.social has a new lightsheet for #MicroscopyMonday !💡New England microscopy cores - do you have a system you want advertised? Send us an image! #NESM
🧠: TdTomato Mouse Brain
🔬: #LifeCanvas SmartSPIM, 1.6x obj
🥼: @andystoneimaging.bsky.social
🏛️: Brandeis LMCF (RRID:SCR_02589)
🧠: TdTomato Mouse Brain
🔬: #LifeCanvas SmartSPIM, 1.6x obj
🥼: @andystoneimaging.bsky.social
🏛️: Brandeis LMCF (RRID:SCR_02589)
Fabulous #SmartSPIM video of a mouse spinal cord!
Spinal motor neurons in dazzling detail. ✨
Each green dot is a spinal motor neuron - crucial but rare cells making up just 1% of neurons in the spinal cord. In diseases like ALS, they are selectively damaged, making them of special interest to neuroscientists.
#neuroskyence #FluorescenceFriday
Each green dot is a spinal motor neuron - crucial but rare cells making up just 1% of neurons in the spinal cord. In diseases like ALS, they are selectively damaged, making them of special interest to neuroscientists.
#neuroskyence #FluorescenceFriday
December 12, 2025 at 9:13 PM
Fabulous #SmartSPIM video of a mouse spinal cord!
They used a LifeCanvas tissue processing and #SmartSPIM imaging pipeline to map whole-brain distribution and projections of noradrenaline neurons in the locus coeruleus, a brain region that supplies this neurotransmitter to most of the CNS. (3/5)
September 18, 2026 at 6:11 PM
They used a LifeCanvas tissue processing and #SmartSPIM imaging pipeline to map whole-brain distribution and projections of noradrenaline neurons in the locus coeruleus, a brain region that supplies this neurotransmitter to most of the CNS. (3/5)
I cleared this brain last year with the @lifecanvastech.bsky.social SmartBatch and imaged with their SmartSPIM and I am pretty proud of it. As a computational guy, I was low key shocked that it came out as well as it did on the first try bc I forgot how to pipette
January 29, 2026 at 5:20 PM
I cleared this brain last year with the @lifecanvastech.bsky.social SmartBatch and imaged with their SmartSPIM and I am pretty proud of it. As a computational guy, I was low key shocked that it came out as well as it did on the first try bc I forgot how to pipette
We know #Alzheimers disease drastically alters brain structure. But are there structural benchmarks we can use to test potential drugs? A new study combines MRI & #SmartSPIM microscopy to map brain-wide structural effects of mutations associated with Alzheimer's: bit.ly/46AAYzK 🧵 (1/3)
March 2, 2026 at 6:03 PM
We know #Alzheimers disease drastically alters brain structure. But are there structural benchmarks we can use to test potential drugs? A new study combines MRI & #SmartSPIM microscopy to map brain-wide structural effects of mutations associated with Alzheimer's: bit.ly/46AAYzK 🧵 (1/3)
Sorry for the lack of details. This was taken on a Life Canvas smartSPIM with a 4x objective and a rolling shutter. The sample is a transgenic mouse whose details I don’t have with me now.
November 21, 2024 at 2:30 PM
Sorry for the lack of details. This was taken on a Life Canvas smartSPIM with a 4x objective and a rolling shutter. The sample is a transgenic mouse whose details I don’t have with me now.
Also thank you to @andystoneimaging.bsky.social for shouting out #SmartSPIM in your talk on Brandeis' Light Microscopy Core Facility!
February 12, 2026 at 3:26 PM
Also thank you to @andystoneimaging.bsky.social for shouting out #SmartSPIM in your talk on Brandeis' Light Microscopy Core Facility!
Hi Julia! My lab uses the smartSPIM from Life Canvas Technologies (Boston, Massachusetts) for imaging. This was cleared using their SMARTBATCH+
January 18, 2025 at 4:33 PM
Hi Julia! My lab uses the smartSPIM from Life Canvas Technologies (Boston, Massachusetts) for imaging. This was cleared using their SMARTBATCH+
Clip from her talk: AAVretro-ArgiNLS-DIO-oScarlet in red, AAV-ArgiNLS-GFP in green (both in the NAc) of a vlgut2-Cre transgenic. Imaged at 1.6x with a SmartSPIM LSFM. Rendered with Arivis.
This is the native (non stained) signal following SHIELD clearing.
Constructs at www.addgene.org/Sam_Golden/
This is the native (non stained) signal following SHIELD clearing.
Constructs at www.addgene.org/Sam_Golden/
December 12, 2024 at 11:49 PM
Clip from her talk: AAVretro-ArgiNLS-DIO-oScarlet in red, AAV-ArgiNLS-GFP in green (both in the NAc) of a vlgut2-Cre transgenic. Imaged at 1.6x with a SmartSPIM LSFM. Rendered with Arivis.
This is the native (non stained) signal following SHIELD clearing.
Constructs at www.addgene.org/Sam_Golden/
This is the native (non stained) signal following SHIELD clearing.
Constructs at www.addgene.org/Sam_Golden/
This was labeled using Life Canvas’ new RADIANT SMARTBATCH+ devices and imaged on a modified smartSPIM at 4x and 15X.
November 20, 2024 at 2:32 PM
This was labeled using Life Canvas’ new RADIANT SMARTBATCH+ devices and imaged on a modified smartSPIM at 4x and 15X.
For #WorldAlzheimersMonth, we’re highlighting a breakthrough 2026 study that shows the power of tissue clearing and SmartSPIM light sheet microscopy to improve preclinical models: www.nature.com/articles/s41...
@davidashbrook.bsky.social
@davidashbrook.bsky.social
September 4, 2026 at 5:06 PM
For #WorldAlzheimersMonth, we’re highlighting a breakthrough 2026 study that shows the power of tissue clearing and SmartSPIM light sheet microscopy to improve preclinical models: www.nature.com/articles/s41...
@davidashbrook.bsky.social
@davidashbrook.bsky.social
In a new Nature study, Dr. @gabineiman.bsky.social et al. developed an in vitro screening method to predict transfection efficacy of LNP-mRNA complexes in the heart, validating it with #SmartSPIM imaging in whole mouse hearts. Check out the paper: bit.ly/444P0rT
@qb3-berkeley.bsky.social
@qb3-berkeley.bsky.social
November 11, 2025 at 5:50 PM
In a new Nature study, Dr. @gabineiman.bsky.social et al. developed an in vitro screening method to predict transfection efficacy of LNP-mRNA complexes in the heart, validating it with #SmartSPIM imaging in whole mouse hearts. Check out the paper: bit.ly/444P0rT
@qb3-berkeley.bsky.social
@qb3-berkeley.bsky.social
#MicroscopyMonday Axons and apical dendrites go in CA1 of mouse Hippocampus. Raw image was acquired w\ a LifeCanvas SmartSPIM light-sheet 🔬 and deconvolved and depth-colored in 3D.
Thanks Matthias Klumpp, @uniheidelberg.bsky.social for joining the previous Image Contest!
Join: svi.nl/ImageContest
Thanks Matthias Klumpp, @uniheidelberg.bsky.social for joining the previous Image Contest!
Join: svi.nl/ImageContest
November 24, 2025 at 10:47 AM
#MicroscopyMonday Axons and apical dendrites go in CA1 of mouse Hippocampus. Raw image was acquired w\ a LifeCanvas SmartSPIM light-sheet 🔬 and deconvolved and depth-colored in 3D.
Thanks Matthias Klumpp, @uniheidelberg.bsky.social for joining the previous Image Contest!
Join: svi.nl/ImageContest
Thanks Matthias Klumpp, @uniheidelberg.bsky.social for joining the previous Image Contest!
Join: svi.nl/ImageContest
Dr. Khalil Ramadi's lab at NYU Abu Dhabi used the LifeCanvas pipeline to develop CORAL, an ingestible device to non-invasively sample the small intestine #microbiome. The #SmartSPIM video below shows blood vessels (pink) & epithelial cells (blue) in rat duodenum. Check out the paper: bit.ly/3JncRfA
November 5, 2025 at 3:56 PM
Dr. Khalil Ramadi's lab at NYU Abu Dhabi used the LifeCanvas pipeline to develop CORAL, an ingestible device to non-invasively sample the small intestine #microbiome. The #SmartSPIM video below shows blood vessels (pink) & epithelial cells (blue) in rat duodenum. Check out the paper: bit.ly/3JncRfA
How did vertebrates evolve kidneys? Pascal Schmidt et al. used #SmartSPIM light sheet microscopy to visualize kidney development in the catshark by mapping expression of the early pronephric marker gene PAX2, revealing a somitic origin: www.science.org/doi/full/10....
Unraveling the origin of the vertebrate kidney: Emergence from a somitic compartment
The pronephros evolves from a somitic compartment in early vertebrates.
www.science.org
July 8, 2026 at 2:39 PM
How did vertebrates evolve kidneys? Pascal Schmidt et al. used #SmartSPIM light sheet microscopy to visualize kidney development in the catshark by mapping expression of the early pronephric marker gene PAX2, revealing a somitic origin: www.science.org/doi/full/10....