New in RSC Chemical Biology: SpyBLI — a fast, low-cost way to measure binding kinetics straight from crude samples. No purification, no cloning, data in under 24h. A game-changer for binder engineering!
DOI doi.org/10.1039/D5CB...
#ProteinEngineering #SpyBLI
DOI doi.org/10.1039/D5CB...
#ProteinEngineering #SpyBLI
July 18, 2025 at 2:53 PM
New in RSC Chemical Biology: SpyBLI — a fast, low-cost way to measure binding kinetics straight from crude samples. No purification, no cloning, data in under 24h. A game-changer for binder engineering!
DOI doi.org/10.1039/D5CB...
#ProteinEngineering #SpyBLI
DOI doi.org/10.1039/D5CB...
#ProteinEngineering #SpyBLI
RRIDs were included in this None paper. Thanks for making your methods matter! #methodsmatter #OpenScience #OpenScience
The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples
doi.org
July 23, 2025 at 7:01 AM
RRIDs were included in this None paper. Thanks for making your methods matter! #methodsmatter #OpenScience #OpenScience
The panel of additives that we devised to optimise cell-free protein synthesis #CFPS using digital microfluidics
www.nuclera.com/resource-lib...
It optimised cell-free scFv synthesis, evaluating affinity without purification using biolayer interferometry (BLI) www.biorxiv.org/content/10.1...
www.nuclera.com/resource-lib...
It optimised cell-free scFv synthesis, evaluating affinity without purification using biolayer interferometry (BLI) www.biorxiv.org/content/10.1...
The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples
Accurate measurements of binding kinetics, encompassing equilibrium dissociation constant (KD), association rate (kon), and dissociation rate (koff), are critical for the development and optimisation ...
www.biorxiv.org
March 22, 2025 at 12:00 PM
The panel of additives that we devised to optimise cell-free protein synthesis #CFPS using digital microfluidics
www.nuclera.com/resource-lib...
It optimised cell-free scFv synthesis, evaluating affinity without purification using biolayer interferometry (BLI) www.biorxiv.org/content/10.1...
www.nuclera.com/resource-lib...
It optimised cell-free scFv synthesis, evaluating affinity without purification using biolayer interferometry (BLI) www.biorxiv.org/content/10.1...
Youngsters got no patience these days. Now it's buy your DNA, in vitro express, SpyBLI for Kd, voilà!
March 20, 2025 at 11:38 PM
Youngsters got no patience these days. Now it's buy your DNA, in vitro express, SpyBLI for Kd, voilà!
The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples https://www.biorxiv.org/content/10.1101/2025.03.06.641810v1
March 11, 2025 at 9:08 PM
The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples https://www.biorxiv.org/content/10.1101/2025.03.06.641810v1
The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples https://www.biorxiv.org/content/10.1101/2025.03.06.641810v1
March 11, 2025 at 9:03 PM
The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples https://www.biorxiv.org/content/10.1101/2025.03.06.641810v1