#T6SS
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drdorsalomon.bsky.social
🧵🦠New paper out in mSystems! We mapped the pan-genome #T6SS effector arsenal of #Pantoea agglomerans. The surprise: its weapons live in dynamic genomic armories that mix antibacterial attack with anti-phage defense. 1/n
journals.asm.org/doi/10.1128/...
Pantoea agglomerans T6SS effectors reside in hotspots with combinatorial antibacterial and antiviral arsenals | mSystems
Bacteria inhabiting competitive environments must simultaneously contend with rival bacteria and bacteriophage predators. Here, we show that Pantoea agglomerans, a species with both pathogenic and bio...
journals.asm.org
November 3, 2026 at 10:58 AM
drdorsalomon.bsky.social
Congrats to the first author Ksenia Leo, and thanks to our collaborators @eranbosis.bsky.social and the Qimron lab. Stay tuned for more cool #T6SS #Pantoea stories coming soon. /end
Michael Scott Says Stay Tuned
ALT: Michael Scott Says Stay Tuned
static.klipy.com
November 3, 2026 at 10:58 AM
drdorsalomon.bsky.social
Mining the armories, we validated two new players: Pse15 (another #T6SS effector) and Juno (a widespread anti-phage defense family; aka VPA1262). “Genomic armories” appear to be a general feature of the bacterial accessory genome. 5/n
November 3, 2026 at 10:58 AM
drdorsalomon.bsky.social
Using proteomics + comparative genomics, we found the largest #T6SS effector arsenal tied to a single system to date: 23 effectors across 15 toxin families. Almost all are “orphans”, encoded outside the main T6SS cluster, scattered across the chromosome and megaplasmids. 2/n
November 3, 2026 at 10:58 AM
drdorsalomon.bsky.social
No effector is universal across P. agglomerans, and these orphans reside in variable genomic “hotspots” that cycle through diverse cargo between strains. Some hotspots carry 160+ distinct cargo versions; entire T6SS clusters or prophages can swap a single effector. 3/n
November 3, 2026 at 10:58 AM
drdorsalomon.bsky.social
The striking part: the hotspots are “genomic armories” co-locating offensive #T6SS effectors with defensive anti-phage systems. We found 155 validated and predicted defense systems in these regions. Offense and defense packed into the same loci, blurring the line between them.4/n
November 3, 2026 at 10:58 AM
rridrobot.bsky.social
The authors included RRIDs in their in Nature Communications paper! RRIDs improve reproducibility in scientific research. #reproducibility #methodsmatter #RRID
A trans-kingdom T6SS effector induces the fragmentation of the mitochondrial network and activates innate immune receptor NLRX1 to promote infection - Nature Communications
Bacteria can affect cellular processes in other bacteria and in eukaryotic cells by injecting effectors using a type VI secretion system (T6SS). Here, Sá-Pessoa et al. describe how a T6SS effector from the bacterial pathogen Klebsiella pneumoniae triggers the fragmentation of the mitochondrial network in eukaryotic cells.
doi.org
October 29, 2026 at 7:00 AM
camolsan.bsky.social
PhD opportunity in Málaga!

We are looking for candidates to apply for an FPU 2026 fellowship and join our lab at IHSM-UMA-CSIC

We study the molecular basis of microbial interactions and plant-baterial interactions focusing on T6SS

🔗 aei.gob.es/ayudas-fpu-2026
#PhD
#T6SS
Ayudas FPU 2026 | Agencia Estatal de Investigación
aei.gob.es
October 20, 2026 at 3:38 PM
ijaa-isac.bsky.social
How does hypervirulent K. pneumoniae worsen liver abscess? Hcp triggers macrophage HMGB1 release and RAGE-dependent endothelial pyroptosis.
www.sciencedirect.com/science/arti...
@isacworld.bsky.social
The T6SS effector Hcp of hypervirulent Klebsiella pneumoniae exacerbates liver abscess by inducing macrophage-HMGB1/RAGE-dependent endothelial pyroptosis
Hypervirulent Klebsiella pneumoniae (hvKP) causes pyogenic liver abscess and fulminant sepsis via vascular endothelial dysfunction and injury. This st…
www.sciencedirect.com
October 20, 2026 at 8:11 AM
drdorsalomon.bsky.social
Check out our recent observation piece 🦠
Artificial genetic augmentation of T6SS activity affects effector repertoire and host toxicity | Microbiology Spectrum journals.asm.org/doi/10.1128/...
Artificial genetic augmentation of T6SS activity affects effector repertoire and host toxicity | Microbiology Spectrum
The type VI secretion system (T6SS), a bacterial toxin-delivery apparatus, has recently been shown to mediate virulence in various marine Vibrio species. Each T6SS delivers a cocktail of toxins into a...
journals.asm.org
October 15, 2026 at 1:08 PM
geiselbiofilm.bsky.social
New in JB: Latario and Ross review the role of type VI secretion system (T6SS) associated genes (TAGs) and place these TAGs into six functional groups.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriol @microsky.bsky.social
October 15, 2026 at 12:24 PM
cnrsbiologie.bsky.social
#ResultatScientifique🔎| Le harpon moléculaire T6SS, utilisé par les bactéries pour éliminer la concurrence, est maintenu par E. coli lorsqu'il offre un avantage, mais souvent désactivé lorsqu’elle devient inutile.

✍️ Eric Cascales

▶️ buff.ly/rNAZZUf
Compétition bactérienne : l’évolution fait le tri dans les armes
Le syst&
buff.ly
October 13, 2026 at 6:00 AM
basler-lab.bsky.social
When Pseudomonas is attacked, it strikes back!
Outer membrane damage triggers a tit-for-tat T6SS retaliation via TagQ/TagR protein complex assembly, activating the PpkA kinase.
Congrats to Maxim & team for uncovering this fascinating mechanism!
www.nature.com/articles/s41...
Membrane damage induces TagQ/TagR complex-mediated H1-T6SS assembly in Pseudomonas aeruginosa - Nature Communications
The H1-T6SS of Pseudomonas aeruginosa assembles in response to membrane damage. Here, the authors show that this response mechanism is regulated by the formation of a complex between the membrane lipo...
www.nature.com
October 8, 2026 at 5:39 AM
drdorsalomon.bsky.social
If you're interested in doing a postdoc with us on #T6SS #MGE, have a look at this opportunity for the prestigious Azrieli postdoc fellowship
🦠
azrielifoundation.org/fellows/inte...
International Postdoctoral Fellowship - The Azrieli Foundation
The Azrieli Fellows Program is an elite group of academics who cultivate a network of leading professionals in Israel and around the world.
azrielifoundation.org
October 8, 2026 at 10:46 AM
zeynepbaharoglu.bsky.social
A type VI secretion ADP-ribosyltransferase Rhs toxin selectively modifies charged tRNAThr(GGU) anticodon loops to block ACC codon decoding academic.oup.com/nar/article/...
A type VI secretion ADP-ribosyltransferase Rhs toxin selectively modifies charged tRNAThr(GGU) anticodon loops to block ACC codon decoding
Abstract. The type VI secretion system (T6SS) is a sophisticated machine that uses a contractile mechanism to deliver antibacterial toxins into neighboring
academic.oup.com
October 3, 2026 at 9:50 AM
hervecelia.bsky.social
A type VI secretion ADP-ribosyltransferase Rhs toxin selectively modifies charged tRNAThr(GGU) anticodon loops to block ACC codon decoding

academic.oup.com/nar/article/...
A type VI secretion ADP-ribosyltransferase Rhs toxin selectively modifies charged tRNAThr(GGU) anticodon loops to block ACC codon decoding
Abstract. The type VI secretion system (T6SS) is a sophisticated machine that uses a contractile mechanism to deliver antibacterial toxins into neighboring
academic.oup.com
October 2, 2026 at 4:01 PM
macerwan.bsky.social
New preprint on bioRxiv from my lab:
How can a single bacterial sRNA coordinate very different competitive strategies?
We show that ArcZ acts as a regulatory hub controlling both antibacterial and antifungal activities in Dickeya solani.
#T6SS #secondarymetabolites #microsky
biorxiv-microbiol.bsky.social bioRxiv Microbiology @biorxiv-microbiol.bsky.social · Sep 30
The ArcZ small RNA activates SlyA production to control contact-dependent antibacterial killing and diffusible antifungal activity in Dickeya solani https://www.biorxiv.org/content/10.64898/2026.09.28.754996v1
September 30, 2026 at 9:14 PM
juliengiraud.bsky.social
Very happy to announce that I have been awarded a 4th year of PhD funding by the @frm-officiel.bsky.social 🎉

I’m truly grateful for this opportunity, which will allow me to continue exploring the fascinating T6SS.

Excited for what’s to come! ✨🌈
September 30, 2026 at 1:55 PM
cascaleslab.bsky.social
New paper out in Nucleic Acids Research @narjournal.bsky.social 🧬 We show that RhsF, a T6SS-delivered Rhs toxin from Chromobacterium violaceum, is an ADP-ribosyltransferase (TreThr) that selectively modifies charged tRNAThr(GGU), blocking ACC codon decoding.

academic.oup.com/nar/article/...
September 29, 2026 at 1:34 PM
asaflevylab.bsky.social
demonstrating a moon-like structural phenotype. You can observe the cell wall unipolar accumulation and degradation in blue. We didn't expect to E. coli looking so weird.

We provide predictions of 19,958 T6SS effector candidates in 2,559 T6SS-encoding bacterial genomes.
September 29, 2026 at 9:30 AM
asaflevylab.bsky.social
Our new preprint is out.
www.biorxiv.org/content/10.6...
We applied an XGBoost algorithm with genomic and biochemical features to detect all T6SS effectors in bacterial genomes.
Next, we validated 3 novel effectors that serve as antibacterial or anti-euk toxins. One of the effectors, from
www.biorxiv.org
September 29, 2026 at 9:30 AM
cascaleslab.bsky.social
This work complements the beautiful study of Jùlia Alves, José da Silva Neto and Sarah Coulthurst published earlier this year:
www.jbc.org/article/S002...
Global identification of Chromobacterium violaceum T6SS effectors reveals an Rhs antibacterial toxin featuring FIX and ADP-ribosyltransferase domains
Bacteria coexist in polymicrobial communities where they engage in complex interactions, including interbacterial antagonism. The environmental bacterial pathogen Chromobacterium violaceum possesses a...
www.jbc.org
September 29, 2026 at 1:34 PM
asaflevylab.bsky.social
We observed that T6SS effectors tend to be longer, more hydrophilic, charged, and ordered, and to reside next to or fused to T6SS genes significantly more than non-T6Es. Common protein domains are depleted from effectors.

Another new effector is likely a non-specific metalloprotease
September 29, 2026 at 9:30 AM
evolvedbiofilm.bsky.social
Will continues on evolution of resistance against T6SS and how multiplicity of T6SS works against resistance evolution
See their PNAS: www.pnas.org/doi/abs/10.1...

#MEBOBacNet
Multiplicity of type 6 secretion system toxins limits the evolution of resistance | PNAS
The bacterial type 6 secretion system (T6SS) is a toxin-injecting nanoweapon that mediates competition in plant- and animal-associated microbial co...
www.pnas.org
September 17, 2026 at 10:11 AM
evolvedbiofilm.bsky.social
Will introduces T6SS and how it helps to deliver toxins
see their previous @plosbiology.org paper
journals.plos.org/plosbiology/...

#EMBoBacNet
The evolution of the type VI secretion system as a disintegration weapon
Bacteria attack each other with poison-tipped spears. This study combines theory and experiments to show that these spears (Type VI Secretion Systems) have evolved to break their targets apart with ly...
journals.plos.org
September 17, 2026 at 10:09 AM