#TMRM
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alicefrolov.bsky.social
Mitochondrial membrane potential heterogeneity with age

I am sharing a data visualization exploring how mitochondrial membrane potential (Δψm) declines.

#MitochondrialBioenergetics #TMRM #MembranePotential #Mitophagy #SingleCellHeterogeneity #OxidativePhosphorylation #MtDNA
#ProtonMotiveForce
Mitochondrial membrane potential heterogeneity with age I am sharing a data visualization exploring how mitochondrial membrane potential (Δψm) declines and becomes more dispersed across the lifespan. The figure, titled "Mitochondrial membrane potential collapses and disperses with age," presents single-cell TMRM (tetramethylrhodamine methyl ester) intensity across four age groups in three tissues. I want to emphasize at the outset that the underlying dataset is simulated; it is constructed to illustrate a trend that is well grounded in the existing literature rather than to report novel empirical measurements. The chart employs a faceted raincloud design, with each tissue occupying its own facet. Within each facet, the four age cohorts are represented by a combination of a half-density (violin) contour, jittered single-cell points, and an overlaid boxplot. This composite format was chosen deliberately to convey both central tendency and the full distributional structure, which is critical here because the phenomenon of interest is not only a shift in the mean but an expansion of intercellular variance. Two features are salient. First, median TMRM intensity declines monotonically with advancing age across all three tissues, consistent with progressive dissipation of the proton-motive force and diminished electron transport chain coupling efficiency. Second, and more importantly, the distributions broaden with age, indicating increasing cell-to-cell heterogeneity in Δψm. This dispersion is compatible with mosaic accumulation of mtDNA mutations, clonal expansion of dysfunctional mitochondrial populations, and stochastic failure of quality-control mechanisms such as mitophagy. I would welcome discussion regarding appropriate normalization strategies for TMRM intensity, particularly the non-quench versus quench mode distinction, and how these affect interpretation of the observed variance structure.
September 20, 2026 at 5:00 PM
ny911canadianangel.bsky.social
youtube.com/watch?v=tmRM...
Love ❤️ Professors comments
Ukraine 🇺🇦 wins 🇺🇦 🏆
Ukraine’s Arctic Drones: The End of Russia’s Safe Haven
YouTube video by Professor Tim Wilson
youtube.com
September 18, 2026 at 9:54 AM
jgp.org
🔬 From our September issue (rupress.org/jgp/issue/15...): Compiled image of HEK cells showing both dark-field imaging and TMRM fluorescent signal.
Colomer-Molera et al. show that structural determinants at KCNE4 position 145 govern Kv1.3 channel function. doi.org/10.1085/jgp....
September 17, 2026 at 2:02 PM
alicefrolov.bsky.social
Figure title: "Mitochondrial membrane potential collapse and dispersion with age"

Subtitle: Per-cell TMRM fluorescence in fibroblasts across four age groups and two tissues

#MitochondrialBioenergetics #TMRM #MembranePotential #SingleCellFunction #mtDNAheteroplasmy #Mitophagy
Figure title: "Mitochondrial membrane potential collapse and dispersion with age" Subtitle: Per-cell TMRM fluorescence in fibroblasts across four age groups and two tissues I am sharing a figure exploring how mitochondrial membrane potential (ΔΨm) distributions shift with donor age at single-cell resolution. The visualization uses a faceted half-violin raincloud design: each facet pairs the smoothed density (the "cloud") of per-cell TMRM fluorescence with jittered raw observations (the "rain"), facilitating simultaneous inspection of central tendency, distributional shape, and the tails that population-averaged assays obscure. Facets are arranged across four age groups and two tissue sources (dermal and pulmonary fibroblasts). A methodological caveat before interpretation: the data are simulated. They are parameterized to reproduce trends reported in the literature rather than to present novel empirical measurements, and are intended to illustrate the analytical framework and expected effect structure. Two features are salient. First, a progressive downward shift in median TMRM signal with age, consistent with age-associated ΔΨm depolarization attributable to declining electron transport chain flux and proton-motive force. Second, and arguably more informative, a marked increase in interquartile spread and bimodality in the older cohorts. This dispersion reflects mitochondrial heterogeneity — the coexistence of polarized and depolarized subpopulations within isogenic cultures — that likely arises from clonal expansion of mtDNA deletions, stochastic mitophagy insufficiency, and asymmetric organelle partitioning across mitotic divisions. I would emphasize that variance itself is a phenotype here. Reporting only mean ΔΨm collapses precisely the signal of interest. The raincloud geometry makes this explicit and, I would argue, should be standard for single-cell functional readouts.
September 8, 2026 at 6:21 PM
scribblinggin.bsky.social
I should add that some of the extras are on YouTube: the full screen tests www.youtube.com/watch?v=63nh... and the Monkees on the Hy Lit Show www.youtube.com/watch?v=TMrM...
www.youtube.com
September 2, 2026 at 5:12 AM
sentimentalist.bsky.social
Name a band and their best song. Not their biggest hit, or their most popular track, their *best* one.

Florence + The Machine: Mother (from How Big, How Blue, How Beautiful)

www.youtube.com/watch?v=TMrm...
August 22, 2026 at 6:59 PM
vixenvana.bsky.social
Did an extra 10 mins before decising I have an early day tmrm and should start to get ready for bed
October 30, 2025 at 1:23 AM
mls1.bsky.social
www.youtube.com/watch?v=tmRM...
January 6 defendants celebrate, reunite with families following pardons at DC Jail
YouTube video by News2Share
www.youtube.com
January 23, 2025 at 12:11 AM
If a political party can't do that, it's just useless.

Beside this is an issue for the Dems for YEARS, and still they hadn't learn the lessons. The Simpsons episode in 1994 shows that (In 1994!)

www.youtube.com/watch?v=TMRm...
The Simpsons - Peanut Factory
YouTube video by Internet King
www.youtube.com
August 2, 2025 at 3:25 AM
youtu.be/yrvf8V_yLGA?...

アルゴさんはすごい。
youtu.be/8IqQJcWKYnI?...

健康なミトコンドリアは、ミトコンドリアを取り囲む脂質二重層の内外間の電位差を示し、電位差が維持されている限り、ミトコンドリア内に蓄積する蛍光色素もあります。 ミトコンドリアのみに局在する蛍光色素であるTMRMについて学んでください。 ミトコンドリアはあらゆる真核細胞にみられる細胞小器官です。 ミトコンドリアはATPを産生するので、細胞の発電所とみなすことができます。
youtu.be
November 9, 2025 at 6:22 AM
ss1ll13stt.bsky.social
I’m lowkey not excited for tmrm. I’m usually never dreading school over a person but now I am all because of freaking last night… ughhhhh
September 21, 2025 at 5:22 PM
ricardocabral61.bsky.social
youtube.com/watch?v=tmRM...
Uma divisão israelense foi mobilizada!!!
YouTube video by Geopolítica e História Militar
youtube.com
March 18, 2026 at 12:25 PM
undomondo.bsky.social
Our wonderful Father Dionisios Tabakis (The Father who made Paradise Metal) is now making Orthodox hip-hop. www.youtube.com/watch?v=tmrM...
Psalter to hip hop beat (ρυθμός αρχαίος Τροχαϊκός)
YouTube video by f.Dionisios Tabakis-Paradise metal
www.youtube.com
May 26, 2026 at 4:52 PM
thechrispaduan.bsky.social
m.youtube.com/watch?v=TMRm...
The Simpsons - Peanut Factory
YouTube video by Internet King
m.youtube.com
March 26, 2026 at 4:25 AM
phartonium.bsky.social
Bon anniv ! J'ai tenté de lister leurs initiales (TMRM, comme... Teenage Mutant Rinja Murtles?), d'additionner les numéros de Pokédex national (0157+0596+0018+0289 = 1060, ça fait vieux), de comparer leurs dates de sorties, je sèche. 🤔
March 24, 2025 at 9:08 AM
geminidivide.bsky.social
I'm just on a kick music.youtube.com/watch?v=TmrM...
ak-gigabab_9122
YouTube video by Vertigoaway - Topic
music.youtube.com
December 22, 2024 at 11:21 PM
purplekissbr.bsky.social
📹 • Purple Kiss participou do Dance Society do 1theK!!

Engajem muito com o vídeo: www.youtube.com/watch?v=TmrM...

#PURPLE_KISS #플로리
#HEADWAY #ON_MY_BIKE
#댄스소사이어티
퍼플키스 (PURPLE KISS) - ON MY BIKE | Dance Society | 댄스 소사이어티 | Performance | 4K
YouTube video by 1theK Originals - 원더케이 오리지널
www.youtube.com
October 25, 2024 at 11:55 AM
xipeng1.bsky.social
🔬HBmito Crimson — a membrane-anchored voltage sensor that captures mitochondrial near-field potential (Vnf) in living cells at 1000 fps.⚡ Discovered miniature "flickers" — 1000× smaller than action potentials
📄 doi.org/rf2d
#Mitochondria #SuperResolution #VoltageImaging #Bioimaging #PhotoniX
Imaging mitochondrial electric flickers in intact cells with a membrane-anchored indicator - PhotoniX
Mitochondria are excitable organelles, and their electrophysiological activity across the inner mitochondrial membrane (IMM) plays a critical role in energy metabolism, cell signaling and cell fate regulation. While the transmembrane voltage of the IMM (Δψm) is traditionally measured with Nernstian dyes such as tetramethylrhodamine methyl ester (TMRM), here we introduce HBmito Crimson (HBmito), a novel membrane-anchored voltage sensor to probe mitochondrial near-field potential (Vnf) in intact cells by capturing fluorescence signal variations through rapid optical imaging. Vnf encompasses surface potential and the innermost portion of Δψm. During mitochondrial action potential, HBmito-reported Vnf-related signals show up to a 1.9-fold increase, concurrent with Δψm changes reported by TMRM. At an imaging rate of 1000 frames per second, transient miniature Vnf-related flickers were further revealed. And these events were three orders of magnitude smaller than action potentials and likely reflected the activity of individual mitochondrial ionic channels. These results provide the first optical imaging evidence of fundamental electrophysiological events in intact mitochondria, and highlight a novel approach for studying excitable membranes.
doi.org
July 17, 2026 at 8:34 AM
bguizzet.bsky.social
www.youtube.com/watch?v=TMRm...
The Simpsons - Peanut Factory
YouTube video by Internet King
www.youtube.com
June 4, 2025 at 6:24 PM
susanschorn.bsky.social
Who gave this cunt my number.
Screenshot from fundraising text sent by Texas RR Commissioner Chsisti Craddick, featuring a rather ghastly photo of her superimposed on a red background with a "Conservative Bluprint" logo that includes a square shape, criss-crossed with random lines, that appears to have been drawn with a crayong. Text reads "I've proven | can deliver for conservatives. And I have a blueprint to do it again at the Comptroller's office. As Railroad . . ." Botto of same fundraising text, ending " . . . auditing spending, modernizing systems, and protecting your tax dollars. See how I'Il get it done: txtchristi.com/ tmRM TK -Christi Craddick Stop to End" Followed by my reply, "Fuck off, you fascist bitch."
August 15, 2025 at 11:28 PM
davidfinlaytcd.bsky.social
Caution : Expression of p-glycoprotein (ABCB1b) affects mitochondrial staining with MTG and TMRM

1-3k copies in NK cells.

Assess mitochondrial mass using multiple methods.
-10-nonyl acridine orange (NA0) stains mito-specific lipid cardiolipin,
-Antibody staining of proteins like ATP5b
October 5, 2025 at 10:49 PM
alicefrolov.bsky.social
New visualization: Age-Related Erosion of Mitochondrial Membrane Potential Across Tissues

I am sharing a figure intended to communicate a well-documented bioenergetic trend.

#Mitochondria #Bioenergetics #Aging #MembranePotential #TMRM #Senescence #CellBiology #DataVisualization
New visualization: Age-Related Erosion of Mitochondrial Membrane Potential Across Tissues I am sharing a figure intended to communicate a well-documented bioenergetic trend rather than a novel empirical result. The data displayed are simulated, generated to reflect distributional patterns consistently reported in the aging literature; they should not be interpreted as a specific experimental cohort. The figure ("TMRM-based ΔΨm distributions decline and disperse with advancing age (simulated cohort)") uses a violin-style plot to represent mitochondrial membrane potential (ΔΨm) inferred from tetramethylrhodamine methyl ester (TMRM) fluorescence across several tissue compartments and age strata. TMRM accumulates in the mitochondrial matrix as a function of ΔΨm in accordance with the Nernst relationship, providing a ratiometric proxy for the proton-motive force established by the electron transport chain. Two features are deliberately emphasized. First, the central tendency of each distribution shifts toward lower ΔΨm with advancing age, consistent with diminished respiratory chain coupling efficiency, accumulation of mtDNA lesions, and reduced complex I and IV activity. Second, and mechanistically important, the distributions broaden with age, reflecting increased cell-to-cell heterogeneity. This dispersion is compatible with mosaic patterns of mitochondrial dysfunction, in which subpopulations of cells sustain depolarization while others remain relatively preserved, plausibly linked to stochastic clonal expansion of deleterious mtDNA variants. I chose the violin format precisely because boxplots or bar summaries obscure this variance structure, which I regard as biologically informative rather than nuisance noise. Tissue-specific differences in baseline ΔΨm and rate of decline are illustrated to prompt discussion of differential vulnerability in post-mitotic versus proliferative compartments.
July 22, 2026 at 5:11 PM
vegan3.bsky.social
Omg lunatics.
m.youtube.com/watch?v=tmRM...
January 6 defendants celebrate, reunite with families following pardons at DC Jail
YouTube video by News2Share
m.youtube.com
January 23, 2025 at 2:32 AM