#ToBRFV
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kamounlab.bsky.social
Real-time tracking of Tomato brown rugose fruit virus (ToBRFV) outbreaks using Nextstrain (v3) nextstrain.nrcnvwa.nl/ToBRFV/20220...
March 4, 2024 at 12:11 PM
kamounlab.bsky.social
High resistance against ToBRFV

HYRULE RZ F1 | Cherry tomato | Vegetable seeds | Rijk Zwaan www.rijkzwaan.co.uk/tomato/HYRUL...
HYRULE RZ F1 | Cherry tomato | Vegetable seeds | Rijk Zwaan
ToBRFV resistant cherry tomato with an impressive combination of production and flavour. Perfect single trusses. Order your professional tomato seeds online!
www.rijkzwaan.co.uk
December 7, 2024 at 1:11 PM
solidevidence.bsky.social
What plant viruses you may ask? The most prominent one is a virus called Tomato Brown Rugose Fruit Virus (ToBRFV).

This was true everywhere in the country.

Americans eat a lot of tomatoes.
5/
October 31, 2025 at 12:19 PM
solidevidence.bsky.social
What’s the rest of the sequence? Mostly rRNA, and other viruses.
The most prevalent *known* virus is Tomato Brown Rugose Fruit Virus (ToBRFV), which is present year round.
5/
October 24, 2025 at 4:44 PM
solidevidence.bsky.social
The ironic thing is that ToBRFV supposedly isn’t even present in the US, so it presumably comes from imported tomatoes and tomato products.
en.wikipedia.org/wiki/Tomato_...
6/
Tomato brown rugose fruit virus - Wikipedia
en.wikipedia.org
October 24, 2025 at 4:44 PM
kamounlab.bsky.social
ToBRFV vaccine 🤦‍♂️ www.hortidaily.com/article/9476...
Belgian tomato grower raided on suspicion of using prohibited ToBRFV vaccine
Past Tuesday a raid took place at a tomato-growing company in Rijkevorsel, Belgium. The grower is suspected of developing and using a prohibited vaccine against the Tomato brown rugose fruit…
www.hortidaily.com
February 12, 2025 at 4:46 PM
ucdavisplants.bsky.social
🌱 From Plant Biotechnology Journal: The N gene from Nicotiana glutinosa confers ToBRFV resistance in #tomato at 22°C but loses efficacy at 30°C. (Savithramma P. Dinesh-Kumar)
▶️ onlinelibrary.wiley.com/doi/10.1111/...
#PlantImmunity #Virology
ToBRFV-induced symptoms and virus accumulation in infected tomato and tobacco plants at 22 °C, Trial 1. (a) ToBRFV-induced HR (hypersensitive response) local lesions appeared on all 11 inoculated tomatoNN leaves at 4 days post-inoculation (4 dpi), whereas no lesions appeared on inoculated leaves of 11 VF36, Tomato::Tm-22, or Moneymaker plants at 4 dpi. (b) Systemic leaves of 11 ToBRFV-infected tomatoNN were asymptomatic at 14 dpi, whereas leaves of VF36 and Moneymaker displayed mosaic and rugose patterns, and tomato::Tm22 leaves displayed rugose and shoestring growth. (c) ToBRFV-induced HR lesions on 11 inoculated Samsun NN plants at 4 dpi but not on inoculated Samsun nn plants. Systemic leaves of ToBRFV-infected Samsun NN plants remained asymptomatic at 14 dpi, whereas leaves of Samsun nn exhibited mosaic disease.
July 6, 2025 at 7:00 PM
solidevidence.bsky.social
What’s sort of surprising is that ToBRFV isn’t even in US tomatoes.
That explains why we see it year round though. These are probably from imported tomatoes and tomato products.
6/
en.wikipedia.org/wiki/Tomato_...
Tomato brown rugose fruit virus - Wikipedia
en.wikipedia.org
October 31, 2025 at 12:19 PM
rtds-group.bsky.social
Our #VIRTIGATION project held a session on new control strategies of the #PlantVirus #ToBRFV at the prestigious #IAPV25 conference. With inspiring presentations from leading #PlantVirologists and a lively panel debate, the session outlined new perspectives for #ToBRFV control: www.virtigation.eu
April 8, 2025 at 2:45 PM
hgpennington.bsky.social
In 2023 I painted tomato brown rugose fruit virus (ToBRFV) symptoms.

www.hgpenningtonart....
The painting shows a tomato stem, with one cluster of tomatoes and one compound leaf. The tomatoes are deformed and blotchy, with pale green/yellow patches. The leaves show mosaic symptoms, with mottling in various shades of green, bulges and deformations.
February 24, 2025 at 11:59 AM
jxbotany.bsky.social
🧬 TECHNICAL INNOVATION 🧬

CRISPR/Cas technology is an emerging tool for identifying nucleic acid sequences.

Hak et al. present a user-friendly, extraction-free, rapid protocol for specific on-site detection of plant viruses using CRISPR/Cas13a.

🔗 doi.org/10.1093/jxb/...
#PlantScience 🧪
Fig. 5 (shortened, full legend in paper): Development of a simplified approach for on-site Cas13a-based diagnostics. (A) Cas13a-mediated detection of tomato brown rugose fruit virus (ToBRFV) in serial dilutions of crude RNA samples from ToBRFV-infected plants diluted in RNA from healthy plants. Viral RNA can be detected in a 1:5 dilution using this method. (B) Equipment required for on-site diagnostics includes dry bath, vortex, portable fluorescence viewer, a pipette, and test tubes. (C) Schematic illustration of virus detection using a portable fluorescence viewer and a cellular phone camera. (D) Mobile phone camera image of serial dilutions of crude RNA from ToBRFV-infected plants. (E) Samples are converted to grayscale. (F) Fluorescence intensity is quantified using ImageJ.
December 2, 2025 at 2:11 PM
bkrenz.bsky.social
New month, new plant virus!
After #ToBRFV in March, we spotlight #GRBV – Grapevine Red Blotch Virus – for April 2025
It’s a DNA virus from the Geminiviridae family, threatening wine & table grape production!
doi.org/10.1371/jour...

#PlantVirology #geminivirus
Grapevine red blotch disease: A comprehensive Q&A guide
doi.org
April 2, 2025 at 9:10 AM
hgpennington.bsky.social
In 2023 I painted tomato brown rugose fruit virus (ToBRFV) symptoms.

www.hgpenningtonart....
The painting shows a tomato stem, with one cluster of tomatoes and one compound leaf. The tomatoes are deformed and blotchy, with pale green/yellow patches. The leaves show mosaic symptoms, with mottling in various shades of green, bulges and deformations.
March 4, 2026 at 11:00 AM
plantdiseasej.bsky.social
Nidà M. Salem et al. establish gradual heat treatment as a robust, nonchemical, and commercially viable strategy to manage tomato brown rugose fruit virus in tomato seeds and highlight the critical importance of bioassays in certifying seed health: https://doi.org/10.1094/PDIS-10-25-2187-RE
Fig. 3. Transmission electron microscopy reveals virion degradation. A, Heat-treated, tomato brown rugose fruit virus (ToBRFV)-contaminated seeds show fragmented and degraded viral particles. B, Untreated, ToBRFV-contaminated seeds show intact, rod-shaped virions. Scale bars = 500 nm.
September 27, 2026 at 3:51 AM
jxbotany.bsky.social
🍅🦠 SPECIAL ISSUE RESEARCH 🦠🍅

Application of in vivo-produced dsRNA specific to tomato brown rugose fruit virus reduces both viral RNA levels and disease symptoms in tomato plants, and triggers mild activation of innate immune responses - Weiss et al.

🔗 doi.org/10.1093/jxb/...
#PlantScience 🧪
Fig. 1.Application of in vitro-produced ToBRFV-specific dsRNA confers protection against ToBRFV in tomato. (A) Schematic illustration of the ToBRFV genome, with the areas selected for in vitro and in vivo dsRNA production. In vitro-produced dsRNA 1 (nt 3295–3693), dsRNA 2 (nt 4760–5133), and dsRNA 3 (nt 5529–5928) targeted three different regions in the viral genome. In vivo dsRNAToBRFV was generated by inserts of 3535-bp and 2550-bp cloned for production of phi6M and S segments, respectively. (B) Representative images of leaves of a healthy, uninfected tomato cv. Moneymaker plants, a ToBRFV-inoculated plant, and a plant co-inoculated with ToBRFV and dsRNA 1 at 14 days post inoculation. Scale bar is 5 cm. (C) Levels of ToBRFV RNA in the leaves shown in (B) as determined by qRT-PCR. Levels are relative to transcripts of TIP41, which served as a reference gene. Significant differences between means were determined using Student’s t-test: *P<0.05; ***P<0.001; ns, not significant (n=7).
February 10, 2026 at 11:37 AM
umweltinstitut.org
Das Tomato Brown Rugose Virus bedroht den weltweiten Tomatenanbau.
Eine Wildtomate aus Peru ist von Natur aus resistent.
Jetzt hat sich das niederländische Unternehmen Enza Zaden genau diese natürliche Resistenz patentieren lassen. Ein klarer Fall von Biopiraterie.
Bild zeigt rote Tomaten an einer Pflanze. Text: „Patent auf Tomaten. Das Tomato Brown Rugose Fruit Virus (ToBRFV) breitet sich rasant aus. Eine Wildtomate aus Peru ist gegen das Virus unempfindlich. Doch auf genau diese natürliche Resistenz wurde ein Patent erteilt.“ Bild zeigt rote Tomaten an einer Pflanze. Text: „Was ist passiert? ToBRFV gilt in vielen Ländern als eine der größten Gefahren für den Tomatenanbau. Eine Wildtomate aus Peru ist resistent gegen das Virus. Resistenzgene sind entscheidend, um neue, robuste Tomatensorten zu entwickeln. Das Europäische Patentamt hat ein Patent auf Gene erteilt, die diese Resistenz vermitteln.“ Foto einer Protestaktion: Mehrere Personen halten große Schilder mit der Aufschrift „Keine Patente auf Saatgut!“ und „No patents on seeds!“. Text: „Fordere mit uns ein umfassendes Verbot von Patenten auf traditionell gezüchtete Pflanzen und Tiere. Sende jetzt eine Protest-Mail an Justizministerin Hubig!“
October 7, 2025 at 12:21 PM
volcaniinstitute.bsky.social
Read this manuscript, which describes the in-vivo production of double-stranded RNA (dsRNA) against ToBRFV, a devastating #virus threatening #tomato #crops worldwide. 🍅 👉 academic.oup.com/jxb/advance-...
July 27, 2025 at 10:33 AM
bkrenz.bsky.social
In 2018, we detected the Tomato brown rugose fruit virus (ToBRFV) - our virus of the month - in 🇩🇪 for the first time. Today, it threatens tomato production worldwide. How did it spread so quickly? A thread 🧵👇
March 3, 2025 at 8:37 AM
roundupnewsbotqld.bsky.social
The Queensland Department of Primary Industries has introduced a movement control order (MCO) from 16 September 2026 to help prevent Tomato Brown Rugose Fruit Virus (ToBRFV) from entering Queensland.
22 September 2026
Burdekin Shire Council
#LGNewsRoundup #BurdekinShireCouncil
BIOSECURITY ALERT – Tomato Brown Rugose Fruit Virus (ToBRFV)
The Queensland Department of Primary Industries has introduced a movement control order (MCO) from 16 September 2026 to help prevent Tomato Brown Rugose Fruit Virus (ToBRFV) from entering Queensland.
www.burdekin.qld.gov.au
September 23, 2026 at 2:33 AM
recordhaber.bsky.social
Yoksa kasıtlı mı?

Gümrüklerdeki denetimler sırasında, yalnızca son 45 içinde 6 defa ToMMV ve ToBRFV virüslü domates tohumu ele geçirildi.

Bu virüsü taşıyan tohumlar ekildiği bölgede sadece domatesleri değil tüm tarım ürünlerini kısa süre içinde yok ediyor.
February 19, 2025 at 6:26 AM
jki-research.bsky.social
Three new #publications from our Institute for #Epidemiology and #Pathogen Diagnostics, including one on Tomato brown rugose fruit virus #ToBRFV by Mareike Johanna Rohde, Annette Niehl, and Heiko Ziebell.

🍅 doi.org/10.1094/PDIS...
🍋 doi.org/10.1111/ppa....
🌿 doi.org/10.1094/PHYT...

#plantscience
Dr Annette Niehl, JKI Institute for Epidemiology and Pathogen Diagnostics. Picture: Johannes Kaufmann/JKI Dr Heiko Ziebell, Team Leader Virology (vegetables, legumes, rapeseed), JKI Institute for Epidemiology and Pathogen Diagnostics. Picture: Johannes Kaufmann/JKI Tomato production: Facility infected with Tomato brown rugose fruit virus. Picture: Heiko Ziebell/JKI Tomatoes showing ToBRFV symptoms offered for sale. Picture: Heiko Ziebell/JKI
May 8, 2025 at 7:55 AM
biorxiv-plants.bsky.social
Contribution of the Tobamovirus resistance gene Tm-1 to control of ToBRFV resistance in tomato https://www.biorxiv.org/content/10.1101/2025.01.20.633895v1
January 22, 2025 at 11:03 PM
rtds-group.bsky.social
Thanks to @aabiologists.bsky.social for organising the conference and enabling #VIRTIGATION to have a prominent forum for discussing the latest advances in #ToBRFV #PlantVirus control
April 8, 2025 at 2:45 PM
kamounlab.bsky.social
Taxonomists now call it Tobamovirus fructirugosum but the virus itself—tomato brown rugose fruit virus (ToBRFV)—remains a plague on the tomato industry. More resistance traits needed 🍅🦠

Great talks by Adrian Fox and Sergio de la Fuente #BSPP26 #PPATH2026 #PlantHealth
September 10, 2026 at 1:33 PM
mpmijournal.bsky.social
Shaokang Zhang et al. identified a single amino acid change at two conserved residues in coat protein that compromises tomato brown rugose fruit virus systemic infection. Read the article to learn more about ToBRFV-encoded proteins at the molecular level: https://doi.org/10.1094/MPMI-07-25-0095-R
Fig. 8. The CPD89 and CPR114 residues are required for virion formation.
March 25, 2026 at 12:06 AM