#antisense
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jorg-vogel-lab.bsky.social
Looking for a new approach to studying or eliminating phages? Check out our study introducing anti-phage ASOs (antisense oligos) out in @Nature today. nature.com/articles/s4158…
September 10, 2025 at 3:40 PM
carolynbertozzi.bskyverified.social
Cool application of in vivo bioorthogonal chemistry from Biogen, imaging biodistribution of ASOs in the brain 🤯

www.science.org/doi/10.1126/...
PET imaging of antisense oligonucleotide distribution in rat and nonhuman primate brains using click chemistry
Intrathecally dosed antisense oligonucleotide distribution can be imaged in rat and nonhuman primate brains using a PET tracer and click chemistry.
www.science.org
May 8, 2025 at 10:12 PM
drmikebooth.bsky.social
We have dramatically increased the activity of splice switching *and* RNase H-active antisense oligonucleotide #ASO therapeutics by conjugating a nuclear importer 🧬💊!

Preprint led by Disha
#chemsky 🧪 @uclchemistry.bsky.social @oxfordchemistry.bsky.social chemrxiv.org/engage/chemr...
Going nuclear: Improved antisense oligonucleotide activity through conjugation with a nuclear importer
Antisense oligonucleotides (ASOs) are a promising class of therapeutics designed to modulate gene expression. Both key mechanisms of action for ASOs operate in the nucleus: splice-switching ASOs modif...
chemrxiv.org
December 5, 2024 at 9:41 AM
eicathomefinn.bsky.social
'Unlike typical gene therapy, which alters a person’s genes to treat a genetic disease, antisense oligonucleotide [RNA] therapy, uses short strands of genetic material to target RNA produced by the gene and reduce how much protein is made.'

Looking for good news. Advances in RNA science & med.
First for RNA therapy: man with rare motor-neuron disease improves after treatment
Gene-targeting treatments could be used to treat other people with rare forms of amyotrophic lateral sclerosis (ALS).
www.nature.com
September 19, 2026 at 9:48 AM
biorxiv-genetic.bsky.social
Antisense transposon insertions into host genes trigger piRNA mediated immunity https://www.biorxiv.org/content/10.1101/2025.07.28.667215v1
August 1, 2025 at 3:32 AM
gerovac.bsky.social
No Genetics? Try ASOs – A non-genetic approach to silence genes at the phage-host interface. We use it to study jumbo phage biology and anti-phage defence.
@jorg-vogel-lab.bsky.social @helmholtz-hiri.bsky.social
@uni-wuerzburg.de @helmholtzhzi.bsky.social
published now in @nature.com
Programmable antisense oligomers for phage functional genomics - Nature
Establishing antisense oligomers as versatile, non-genetic tools to silence phage mRNAs opens applications in basic research and biotechnology, as shown by identifying essential factors for propagatio...
www.nature.com
September 11, 2025 at 9:10 AM
drmikebooth.bsky.social
We have developed an entirely new mechanism of action for gene knockdown -> trafficking of targeted mRNAs to the lysosomal, using lysosomal trafficking oligonucleotides (LyTONs)!

Published in @chemicalscience.rsc.org
Led by Disha Kashyap
#chemsky 🧪
pubs.rsc.org/en/content/a...
Engineering antisense oligonucleotides for targeted mRNA degradation through lysosomal trafficking
Antisense oligonucleotides (ASOs) can modulate gene expression at the mRNA level, providing the ability to tackle conventionally undruggable targets and usher in an era of personalized medicine. A key...
pubs.rsc.org
June 23, 2025 at 8:01 AM
sailorrooscout.bsky.social
strategy to reactivate TRA2β’s poison exon using antisense oligonucleotides (ASOs). These synthetic RNA fragments are designed to bind precisely to the TRA2β RNA, forcing the poison exon back into the sequence. When introduced into cancer cells, ASOs restored the natural SELF-DESTRUCT mechanism.
March 23, 2025 at 4:05 PM
nature.com
Nature research paper: Programmable antisense oligomers for phage functional genomics

go.nature.com/46b1XAQ
Programmable antisense oligomers for phage functional genomics - Nature
Establishing antisense oligomers as versatile, non-genetic tools to silence phage mRNAs opens applications in basic research and biotechnology, as shown by identifying essential factors for propagation of the nucleus-forming jumbo phage ΦKZ such as the RNase H-like protein ΦKZ155.
go.nature.com
September 12, 2025 at 8:14 AM
javierapfeld.bsky.social
That makes antisense
December 5, 2023 at 8:13 PM
ribo.zone
my thoughts on making sense, for manifesto jam
antisense manifesto by ribose
CTGACTCTTA TAAGAGTCAG
ribozone.itch.io
June 13, 2026 at 1:32 PM
eddaschulz.bsky.social
⚠️ The final work of two former PhD students Till @tschwammle.bsky.social and Verena @verenamutzel.bsky.social is out!
➡️⬅️ They dissect how memory can arise from antisense transcription using mathematical modelling 💻, genomics 🧬 and synthetic biology ⚒️! link.springer.com/article/10.1...
Antisense transcription can induce expression memory via stable promoter repression - Genome Biology
Background The capacity of cells to retain a memory of previous signals enables acquisition of unique fates and adaptation to their environment. The underlying gene expression memory can arise from mu...
link.springer.com
January 20, 2026 at 11:02 AM
rm0sher.bsky.social
Had to look up an old paper to get the doi. It still completely boggles my mind that TWO Nobel prizes have been given for small RNAs and this work wasn't recognized by either. Abstract is literally like "yeah, we found these tiny antisense RNAs and think they're probably causing gene silencing"
A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants by Andrew J. Hamilton and David C. Baulcombe Abstract Posttranscriptional gene silencing (PTGS) is a nucleotide sequence–specific defense mechanism that can target both cellular and viral mRNAs. Here, three types of transgene-induced PTGS and one example of virus-induced PTGS were analyzed in plants. In each case, antisense RNA complementary to the targeted mRNA was detected. These RNA molecules were of a uniform length, estimated at 25 nucleotides, and their accumulation required either transgene sense transcription or RNA virus replication. Thus, the 25-nucleotide antisense RNA is likely synthesized from an RNA template and may represent the specificity determinant of PTGS.
September 1, 2025 at 2:51 PM
mulkern.bsky.social
Out now in @natcomms.nature.com: how do bacteria evolve resistance to programmable antisense antibiotics?

www.nature.com/articles/s41...
A systematic identification of resistance determinants to antisense antibiotics suggests adaptation strategies dependent on the delivery peptide - Nature Communications
In this study, authors investigate the evolution of bacterial resistance to antisense peptide nucleic acid (PNA) antibiotics. They observe that this typically occurs mainly by reducing drug uptake via...
www.nature.com
August 12, 2026 at 9:38 PM
olivierduss.bsky.social
My first PhD student‘s, Kavan Gor, paper on 5-color single-molecule imaging of co-transcriptional RNA folding is out in @science.org Advances!

He finds how RNA modification enzymes, antisense oligonucleotides and ribosomal proteins re-route nascent RNA folding.

See more details in EMBL post below!
embl.org EMBL @embl.org · Mar 24
(1/2) EMBL researchers led by @olivierduss.bsky.social have developed a microscopy technique that enables them to observe individual RNA molecules being produced and folding into different shapes.

Learn more: www.science.org/doi/10.1126/...
Illustration showing RNA polymerase and four laser lights. Credit: Daniela Velasco/EMBL
March 25, 2026 at 5:57 PM
natcomms.nature.com
Antibiotic resistance evolution:

A systematic identification of resistance determinants to antisense antibiotics suggests adaptation strategies dependent on the delivery peptide

@mulkern.bsky.social @mgalactus.bsky.social @twincore.bsky.social @helmholtzhzi.bsky.social @helmholtz-hiri.bsky.social
A systematic identification of resistance determinants to antisense antibiotics suggests adaptation strategies dependent on the delivery peptide - Nature Communications
In this study, authors investigate the evolution of bacterial resistance to antisense peptide nucleic acid (PNA) antibiotics. They observe that this typically occurs mainly by reducing drug uptake via mutations in the carrier peptide’s transporter, while resistance to a different carrier peptide stayed low and is easily bypassed by tweaking the drug sequence.
dlvr.it
September 22, 2026 at 3:12 PM
adrianisaacs.bsky.social
First post here! Our latest paper showing CRISPR-CasRx can target both sense and antisense C9orf72 repeats is just out! rdcu.be/d5ADQ co-led by @alexcammack.bsky.social @ukdri.bsky.social @uclqsion.bsky.social
Dual-targeting CRISPR-CasRx reduces C9orf72 ALS/FTD sense and antisense repeat RNAs in vitro and in vivo
Nature Communications - CRISPR-CasRx effectively reduces ALS- and FTD-causing C9orf72 sense and antisense repeat derived RNAs and proteins in cell lines, patient iPSC-neurons and two independent...
rdcu.be
January 8, 2025 at 11:41 AM
nature.com
A rapid and efficient method developed to create stem-cell-based 3D mini-organs derived from the cells of patients offers a way to evaluate personalized treatments using RNA therapies for rare genetic diseases

https://go.nature.com/3POiHpJ
A scalable system using mini-organs to test personalized RNA therapy
Platform to accelerate the testing of antisense oligonucleotides.
go.nature.com
January 25, 2025 at 12:06 PM
malo82.bsky.social
5) Die 2. Art sind Antisense-RNAs (aRNA). Auch diese haben eine komplementäre Sequenz zur Ziel mRNA. Hier erfolgt jedoch kein Abbau, sondern durch die Ausbildung eines mRNA-aRNA-Doppelstrang wird die Translation durch das Ribosom verhindert.
November 10, 2024 at 6:13 PM
natbiotech.nature.com
A systemic screen using programmable antisense oligomers to silence genes of DNA and RNA phages identifies proteins essential for phage propagation. This tool offers potential for optimizing phage therapy. #NBThighlight www.nature.com/articles/s41...
Programmable antisense oligomers for phage functional genomics - Nature
Establishing antisense oligomers as versatile, non-genetic tools to silence phage mRNAs opens applications in basic research and biotechnology, as shown by identifying essential factors for propagatio...
www.nature.com
September 12, 2025 at 9:40 AM
sailorrooscout.bsky.social
The revelation by researchers from The Jackson Laboratory and UConn Health could lead to new RNA-based multi-cancer treatments.

The study has been published in Nature Communications. YES, it is PEER-REVIEWED.
• www.nature.com/articles/s41...
TLDR Cancer disrupts RNA splicing by suppressing poison exons, leading to uncontrolled tumor growth. Researchers at JAX and UConn Health found that targeting TRA2B RNA with antisense oligonucleotides (ASOs) restores this natural kill switch, shutting down cancer growth signals. ASOs offer a promising, highly specific approach for treating aggressive cancers. The discovery could pave the way for a groundbreaking RNA-based multi-cancer treatment. ABSTRACT Upregulated expression of the oncogenic splicing factor TRA2ß occurs in human tumors partly through decreased inclusion of its autoregulatory non-coding poison exon (PE). Here, we reveal that low TRA2ß-PE inclusion negatively impacts patient survival across several tumor types. We demonstrate the ability of splice-switching antisense oligonucleotides (ASOs) to promote TRA2ß-PE inclusion and lower TRA2ß protein levels in pre-clinical cancer models. TRA2ß-PE-targeting ASOs induce anti-cancer phenotypes and widespread transcriptomic alterations with functional impact on RNA processing, mTOR, and p53 signaling pathways. Surprisingly, the effect of TRA2ß-PE-targeting ASOs on cell viability are not phenocopied by TRA2ß knockdown. Mechanistically, we find that the ASO functions by both decreasing TRA2ß protein and inducing the expression of TRA2B-PE-containing transcripts that act as long non-coding RNAs to sequester nuclear proteins. Finally, TRA2B-PE-targeting ASOs are toxic to preclinical 3D organoid and in vivo patient-derived xenograft models. Together, we demonstrate that TRA2ß-PE acts both as a regulator of protein expression and a long-noncoding RNA to control cancer cell growth. Drugging oncogenic splicing factors using PE-targeting ASOs is a promising therapeutic strategy. This research was supported by the National Institutes of Health and the NCI-designated JAX Cancer Center.
March 23, 2025 at 4:01 PM
varechavala.bsky.social
🚨 We're hiring! 🚨

Join us at Biobizkaia 🌍 as part of the MSCA-DN EffecT project! We’re offering a fully funded #PhD position UPV/EHU. 🎓

📌 Focus on:
🔬 #Antisense #oligonucleotides
🧬 #Splicing
🌟 #Rarediseases📄

Apply now: euraxess.ec.europa.eu/jobs/296420
Info here: www.effect-dn.eu/candidates
12 PhDs on Antisense oligonucleotide research to foster the full therapeutic potential of antisense technology across tissues
EFFecT (European Training Program to Foster the Full Therapeutic Potential of Antisense Technology across Tissues) is a Marie Skłodowska-Curie Doctoral Network (2025-2028), offering 12 PhD positions.
euraxess.ec.europa.eu
January 14, 2025 at 10:05 AM
gerovac.bsky.social
Use antisense oligomers to silence phage genes and discover new biology.
@jorg-vogel-lab.bsky.social @helmholtz-hiri.bsky.social @helmholtzhzi.bsky.social @uni-wuerzburg.de
print: www.nature.com/articles/s41...
highlight: www.nature.com/articles/s41...
briefing: www.nature.com/articles/d41...
October 31, 2025 at 8:08 AM
evolvedbiofilm.bsky.social
An antisense RNA regulates production of DnaA and affects sporulation in Bacillus subtilis

PLoSGenetics from Alan Grossman

journals.plos.org/plosgenetics...
An antisense RNA regulates production of DnaA and affects sporulation in Bacillus subtilis
Author summary DnaA is the highly conserved replication initiator and transcription factor found in virtually all bacteria. The synthesis and activity of DnaA are highly regulated, and different types...
journals.plos.org
May 15, 2025 at 10:32 AM
biorxiv-bioinfo.bsky.social
ASOFormer: a Transformer-based model for predicting antisense oligonucleotide efficacy to support therapeutic candidate prioritization https://www.biorxiv.org/content/10.64898/2026.09.18.752788v1
September 24, 2026 at 10:46 PM