Loss of function of canonical BAF induces gain of CTCF and cohesin binding that alters 3D genome architecture and reprograms transcription.

Loss of function of canonical BAF induces gain of CTCF and cohesin binding that alters 3D genome architecture and reprograms transcription.

The animal experimental procedures were conducted in strict compliance with the guidelines established by Weill Cornell Medicine, following the Guide for the Care and Use of Laboratory Animals, and the standards set by the Association for Assessment and Accreditation of Laboratory Animal Care International. The Research Animal Resource Center, Weill Cornell Medicine’s Institutional Animal Care and Use Committee and the Cornell Institutional Animal Care and Use Committee reviewed and approved all procedures for the mouse study under protocols 2011-0031 (principal investigator (PI): D.B.) and 2025-0008 (PI: D.B.). Mice were maintained under a 12-h light–dark cycle at 20–22 °C and 40–60% relative humidity in a specific pathogen-free facility with ad libitum access to food and water. Experiments involved both male and female mice and were sex-matched and age-matched (ranging from 8 to 16 weeks) unless otherwise specified. All experimental groups included both sexes. No sex-based differences or biases were observed. Arid1a (strain 027717), Cg1cre (strain 010611) and Pbrm1 (strain 031875) mice were sourced from The Jackson Laboratory. The CtcfHet (Ctcf+/-Cg1cre/+) mouse strain was a gift from the laboratory of G. Lauvau. The Arid1a strain was back-crossed to the C57BL/6J background for at least six generations before being used in experiments. In...

The animal experimental procedures were conducted in strict compliance with the guidelines established by Weill Cornell Medicine, following the Guide for the Care and Use of Laboratory Animals, and the standards set by the Association for Assessment and Accreditation of Laboratory Animal Care International. The Research Animal Resource Center, Weill Cornell Medicine’s Institutional Animal Care and Use Committee and the Cornell Institutional Animal Care and Use Committee reviewed and approved all procedures for the mouse study under protocols 2011-0031 (principal investigator (PI): D.B.) and 2025-0008 (PI: D.B.). Mice were maintained under a 12-h light–dark cycle at 20–22 °C and 40–60% relative humidity in a specific pathogen-free facility with ad libitum access to food and water. Experiments involved both male and female mice and were sex-matched and age-matched (ranging from 8 to 16 weeks) unless otherwise specified. All experimental groups included both sexes. No sex-based differences or biases were observed. Arid1a (strain 027717), Cg1cre (strain 010611) and Pbrm1 (strain 031875) mice were sourced from The Jackson Laboratory. The CtcfHet (Ctcf+/-Cg1cre/+) mouse strain was a gift from the laboratory of G. Lauvau. The Arid1a strain was back-crossed to the C57BL/6J background for at least six generations before being used in experiments. In...

Genome-wide screens identify several genes, including MAZ, required for CTCF-mediated insulation. MAZ interacts with cohesin, and MAZ motif deletions derepress posterior Hox gene expression, leading to homeotic transformations in mouse.

New research from Memorial Sloan Kettering Cancer Center reveals how cohesin activates distant genes, identifies an immunogenic cell death called baciptosis, links microexon splicing to unexplained

PubMed link

Using proteomics together with an auxin-inducible degron system, Wu et al. identify proteins proximal to MAU2 and uncover genome-wide roles for CDC5L in pre-mRNA processing and cohesin-mediated genome folding in human cells.

Abstract. The spatiotemporal organization of chromatin in the eukaryotic nucleus is fundamental for genome regulation. Chromatin undergoes rapid remodellin

High-resolution real-time imaging of enhancer-promoter communication reveals how the two genomic loci are actively brought together through cohesin-mediated loop extrusion and how E-P interactions are...

High-resolution real-time imaging of enhancer-promoter communication reveals how the two genomic loci are actively brought together through cohesin-mediated loop extrusion and how E-P interactions are temporally coordinated with transcriptional bursts. Unexpectedly, components of the RNA Pol II general machinery form nanoscale clusters at distal enhancers, and embedding the promoter into such clusters boosts GTF activities and triggers burst initiation.

Abstract Available experimental data do not yet permit an unambiguous or comprehensive answer to the question posed in the title. A conventional approach to determining the role of a particular protei...