#dnac
Cellular replisomes are powered by flex-fuel motors for unwinding DNA
To better understand the molecular mechanisms of both replicative helicase loading and translocation in bacteria, we established a single molecule assay that monitors both events using E. coli DnaB and DnaC. We covalently attached a Cy5 fluorophore to the helicase through a surface-exposed point mutation, D290C, which does not affect helicase function (Supplementary Fig. 1A). A 48 knt ssDNA substrate was then generated by force stretching dsDNA lambda DNA tethered between two 4.3 µm beads (in a coupled optical trap/confocal fluorescence microscopy system, the LUMICKS C-trap) and used to capture individual helicases and image their behaviour over time (Fig. 1A, Supplementary Figs. 1B–E). DnaB loading was evidenced by the appearance of single, fluorescent foci between the beads, and was seen only when both nucleotide (ATP) and DnaC were present in the C-trap loading channel (Fig. 1B). Once loaded, helicases were seen to translocate in the reaction channel, moving smoothly over time with little discernable pausing (Fig. 1C, Supplementary Fig. 2A, Supplementary movie 1). All helicases loaded onto a given tether moved in a single direction (presumably 5′→3′, the established polarity for DnaB11), consistent with powered rather than diffusive movement. To assess the specific role of ATP in supporting DnaB loading...
www.nature.com
June 30, 2026 at 2:16 AM
Video of Kate Wolf/ Nina Gerber. "These Times We Are Living In" ...

youtube.com/watch?v=dNac...
Kate Wolf & Nina Gerber 'These times we're living in_HQ.mov
YouTube video by kevanrbrownvideo
youtube.com
June 22, 2026 at 5:03 PM
It's INSANE to me that this was a non-LP single. (His guest spot on the last LL Cool J album was great, too.)
www.youtube.com/watch?v=dNAC...
Busta Rhymes - Don't Touch Me (Throw Da Water On 'Em)
YouTube video by Elesmit
www.youtube.com
June 3, 2026 at 10:04 PM
España supera por primera vez los 22,3 millones de afiliados, tras sumar 232.000 en el segundo mejor mes de mayo de la serie histórica #dnac #españa
España supera por primera vez los 22,3 millones de afiliados, tras sumar 232.000 en el segundo mejor mes de mayo de la serie histórica | Cuadernos Manchegos
España supera por primera vez los 22,3 millones de afiliados, tras sumar 232.000 en el segundo mejor mes de mayo de la serie histórica
www.cuadernosmanchegos.com
June 2, 2026 at 8:08 AM
Omg I missed this update. Thanks DNAC
April 28, 2026 at 8:21 PM
#BO
Modificación del Procedimiento para la Solicitud de Autorización de Importación y/o Exportación de Organismos Acuícolas Vivos.

www.boletinoficial.gob.ar/detalleAviso...
BOLETIN OFICIAL REPUBLICA ARGENTINA - MINISTERIO DE ECONOMÍA DIRECCIÓN NACIONAL DE ACUICULTURA - Disposición 1/2026
BOLETIN OFICIAL REPUBLICA ARGENTINA - MINISTERIO DE ECONOMÍA DIRECCIÓN NACIONAL DE ACUICULTURA - Disposición 1/2026 - DI-2026-1-APN-DNAC#MEC
www.boletinoficial.gob.ar
April 20, 2026 at 10:37 AM
He will need the blessing of the DNac to get anywhere with it. And that won’t happen. He’s basically a republican at this point.
Bernie as tried year after year, and probably would have ended up being a great president but they have fucked him over every time.
March 5, 2026 at 9:55 PM
February 16, 2026 at 2:23 PM
The second verse always brings the tears
Such a beautiful song

www.youtube.com/watch?v=dNac...
Kate Wolf & Nina Gerber 'These times we're living in_HQ.mov
YouTube video by kevanrbrownvideo
www.youtube.com
January 20, 2026 at 9:59 PM
1.1 - New Hire: It’s Jeremy’s first day on the job at DNAC Inc.! Is he prepared for the new position and what exactly was he hired to do? 
Recommend listening: Hunter by San Cisco
Links in the bio
#ghostapocalyptic #comics #comicbooks #webcomic #art #illustration #digitart #artist #sketch #drawing
November 17, 2025 at 2:04 PM
Cisco脆弱性情報、「Medium:Cisco Catalyst Center Cross-Site Scripting Vulnerability」が公開されました。
→ https://sec.cloudapps.cisco.com/security/center/content/CiscoSecurityAdvisory/cisco-sa-dnac-xss-weXtVZ59
November 13, 2025 at 7:32 PM