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joachimgoedhart.bsky.social
Fluorescence Lifetime Imaging in Plants: Practical guidelines for multiplexing, label-free imaging and data analysis by Thomas Ott and team: www.biorxiv.org/content/10.6...
Figure 2. Live-cell multicolour lifetime imaging with two pairs of fluorescent reporters in different spectral windows. A) Two green- (EGFP and NowGFP) and two red- (mCherry and mScarlet) emitting FPs with large lifetime differences (Δτ) were targeted to four different compartments of N. benthamiana leaf epidermal cells (schematic). PM = plasma membrane; Chl = chloroplasts; N = nucleus; ER = endoplasmic reticulum. B) Intensity-only and FastFLIM confocal images of an epidermal cell showing the discrimination of two fluorescent reporters within the green and red channel based on lifetime values (rainbow bar). C) Identification of the fluorescent reporters and mapping of their subcellular distribution based on the Phasor fingerprint. The white dashed box indicates the region shown in the close up in (D)
June 12, 2026 at 12:20 PM
dixieflatline76.bsky.social
Grain

Testshot
Roleicord TLR 1945
lford Delta 3200

#filmphotography #6x6 #mediumformat #squareaspect #fastflim #roleicord
January 21, 2025 at 8:33 PM