This is so far the best imaging of a E4.5 mouse embryo. From our M2 student Théo Boyer!

Cleared with RapiClear (thks @alexandremayran.bsky.social). SOX2 is cyan, GATA6 is magenta, DAPI in gray. Playing with Napari. #fluorescentFriday #teamDICE #Development

ETV2-modified hiPSC-derived Endothelial Cell in microtissue engineering🤠

Vascularized Tumor (Skov3 tumor spheroids) on a chip, perfused with mesothelin-CAR T cells

Vascularized GATA6 hiPSC Liver Organoid

Roger Kamm lab bioRxiv 2025
www.biorxiv.org/content/10.1...

Pioneer factor GATA6 promotes colorectal cancer
through 3D genome regulation

www.science.org/doi/pdf/10.1...

GATA6 Mediates Endoderm and Mesoderm Progenitor Fate Driven by WNT and NODAL Signaling https://www.biorxiv.org/content/10.1101/2025.06.20.660704v1

Peritoneal cavity-derived GATA6+ macrophages inhibit fibrosis through IL33 in endometrium
doi.org/10.1101/2025...

www.sciencedaily.com/releases/202...

Scientists have identified a molecular switch that may explain how colorectal cancer becomes deadly. When levels of a gene-regulating factor called GATA6 drop, cancer cells can shed their identity and transform into highly adaptable, fetal-like cells capable of establishing new tumors in the liver.

Our findings suggest that #miR-10b-5p regulates early #adipogenesis by influencing cell fate bias toward the #paraxialMesoderm lineage through modulation of the #Gata6 / #Bmp2 signaling axis.
#NTU_Metabolic #SHIMR_NTU
@ntumeta.bsky.social @mark-christian-lab.bsky.social @ntuyourlibrary.bsky.social

A rationale for combining KRAS/ERK inhibitors with standard-of-care chemotherapies in pancreatic cancer

David M. Virshup & team report on the suppression of the master regulator of differentiation and chemosensitivity—GATA6 expression—in PC models: doi.org/10.1172/JCI1...

This review elucidates the indispensable role of GATA-binding protein 6 (GATA6) in pancreas physiology and pathology and explores its potential as a novel #biomarker and a promising therapeutic target for #PancreaticDuctalAdenocarcinoma. #medsky

#OpenAccess: www.sciencedirect.co...

Gata6-independent, −165 kb Zeb2 enhancer–dependent large cavity macrophages in peritoneal fluid can replenish mesothelial macrophages @sciimmunology.bsky.social @washumedicine.bsky.social
www.science.org/doi/10.1126/...

and this signals into the PE cell and turns off nanog and sox2. (see my Thread 1 for more info on nanog and sox2). It also activates genes sox17 and gata6. Mainly keep in mind that primitive endoderm markers are oct4 and sox17. (5/n)

To do so, we generated transgenic mESC lines in which different types of synthetic enhancers (i.e. “typical” enhancer, CTCF-associated enhancer, 4x enhancer cluster) were built upon the same “core” enhancer and inserted at the same distance from a developmental gene (i.e. 100 Kb from Gata6)

Low GATA6 appears to switch colorectal cancer cells into a more primitive, metastasis-ready state. In mouse models, deleting GATA6 increased liver metastases with little effect on primary tumor growth. doi.org/hb8f7w

Inhibiting the GATA6 protein in colon cancer models reduces tumor growth and improves survival, highlighting its potential as a therapeutic target for colon cancer treatment. doi.org/g86mbw

Fate specification triggers a positive feedback loop of TEAD–YAP and NANOG to promote epiblast formation in preimplantation embryos

Read this Research Article by Naoki Hirono, Masakazu Hashimoto, Hiromi Shimojo & Hiroshi Sasaki:
https://journals.biologists.com/dev/article/152/1/dev203091/364965/

Oncogenic KRAS/ERK/JUNB signaling suppresses differentiation regulator GATA6 in pancreatic cancer

Meantime, EPI cells send FGF ligand to other cells but they don’t have the receptor for it themselves. So there is no way to suppress nanog & sox2. Nanog is free to actually suppress gata6 (so *really* preventing a PE fate)! Mainly keep in mind from this that epiblast markers are oct4 & sox2. (6/n)