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VN: local LTP at single synapse (Glu uncaging) induces bigger spine head, and STED reveals shorter and thicker spine neck #iphria
December 26, 2024 at 4:53 PM
christletx.bsky.social
VN: STED reveals that "stubby" dendritic spines are an imaging artifact caused by the limited resolution of 2-photon #iphria
December 26, 2024 at 4:53 PM
christletx.bsky.social
And the last speaker of the day is Valentin Nägerl (Bordeaux), who uses STED to study synapses in brain slices #iphria
December 26, 2024 at 4:53 PM
christletx.bsky.social
MB: Define metric for STED image quality by combining brightness (sum of pixels) and sharpness (2nd moment of Fourier transform) #iphria
December 26, 2024 at 4:53 PM
christletx.bsky.social
MB combines adaptive optics to STED microscopy. Liquid crystal SLM replaces phase plate + corrects aberrations #iphria
December 26, 2024 at 4:53 PM
christletx.bsky.social
Martin Booth (Oxford): Adaptative optics for microscopy and nanoscopy #iphria
December 26, 2024 at 4:53 PM
christletx.bsky.social
AF: This allows live-cell SIM, with good temporal and spatial resolution #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
AF: classical SIM uses 3x3 images for SIM, but it is possible to use just 4 (3 grid orientations + 1 widefield) #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
Alexandra Fragola (ESPCI Paris) on live-cell SIM #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
Kay Gruenwald (Oxford) uses flash freezing & cryo-EM to image virus-host interaction #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
RO also speaks about multi-plane approaches for Z-resolution, 3D tracking in thick samples #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
RO's imaging modality (UAIM) allows longer SPT by lowering illumination levels #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
Next is Raimund Ober: using high mag and EMCCD cameras to get ultra high single molecule localization precision #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
SC talks about her next gen algorithm, faster than 3B, somehow between 3B and PALM/STORM for acquisition/processing speed compromise #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
SC explaining the 3B super resolution method. Well I still don't get what Bayesian statistics are #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
Susan Cox on accelerating the speed of localization microscopy and use it on live cells #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
WB uses the cryo-FIB to study synapses with correlated fluo / cryo EM on cultured neurons #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
WB: tomography using cryo-focused ion beam to section vitrified eukaryotic cells. Mills a thin lamella inside the cell #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
WB shows tomography EM of actin filaments in synapses and Listeria comets #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
Wolfgang Baumeister on cryoelectron microscopy study of the 26S proteasome organization #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
XD switches to sptPALM of transcription factors: tracking and using angle correlation between trajectory steps to detect constrains #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
XD shows data from Cisse Science 2013, SR study of transient RNA PolII clusters around promoters, lifetime ~5s #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
Now Xavier Darzacq talks about RNA-PolII and transcription factories. Live-cell images with 25s sliding-window PALM reconstructions #iphria
December 26, 2024 at 4:52 PM
christletx.bsky.social
If you guys are fed up with my LT flood: you can mute the #iphria hashtag, or wait until my battery runs out, which should not take long
December 26, 2024 at 4:52 PM
christletx.bsky.social
JLS on use of PALM for protein clustering/counting in clusters (correlation-based PC-PALM) for example Renz PNAS 2012 #iphria
December 26, 2024 at 4:52 PM