We make🛠️these libraries via METa assembly (previous twitter thread👇) using tagmentation to fragment DNA/add mosaic end tags on all fragments. This lets us use assembly cloning to *efficiently* ligate fragments into plasmids with a mosaic ends cloning site.
x.com/TerenceCroft...
x.com/TerenceCroft...
Terence Crofts on X: "I am thrilled that my METa assembly paper with collaborators @ericamhartmann and @alexmcfarland_ is online now at @mSystemsJ: https://t.co/Na6iDJTnYV We show that functional metagenomic libraries (FMLs) can be made using 20-fold less DNA and with 80-fold greater efficiency 1/n https://t.co/3MO7IgScNY" / X
I am thrilled that my METa assembly paper with collaborators @ericamhartmann and @alexmcfarland_ is online now at @mSystemsJ: https://t.co/Na6iDJTnYV We show that functional metagenomic libraries (FMLs) can be made using 20-fold less DNA and with 80-fold greater efficiency 1/n https://t.co/3MO7IgScNY
x.com
September 11, 2025 at 3:56 PM
We make🛠️these libraries via METa assembly (previous twitter thread👇) using tagmentation to fragment DNA/add mosaic end tags on all fragments. This lets us use assembly cloning to *efficiently* ligate fragments into plasmids with a mosaic ends cloning site.
x.com/TerenceCroft...
x.com/TerenceCroft...
Today is the last day to apply to be #mSystemsJ Senior Editor!
Deadline approaching! 🚨 #mSystemsJ seeks 6 Senior Editors to bring the journal into the future! ⏳Apply by March 23 to shape the direction of systems biology: asm.social/2km #ASMJournals
March 24, 2025 at 6:21 PM
Today is the last day to apply to be #mSystemsJ Senior Editor!
Deadline approaching! 🚨 #mSystemsJ seeks 6 Senior Editors to bring the journal into the future! ⏳Apply by March 23 to shape the direction of systems biology: asm.social/2km #ASMJournals
March 21, 2025 at 6:32 PM
Deadline approaching! 🚨 #mSystemsJ seeks 6 Senior Editors to bring the journal into the future! ⏳Apply by March 23 to shape the direction of systems biology: asm.social/2km #ASMJournals
Very happy that MICOM is now published @mSystemsJ. Want to take look at functional consequences of microbial composition? This might be for you https://msystems.asm.org/content/5/1/e00606-19
January 29, 2025 at 8:27 AM
Very happy that MICOM is now published @mSystemsJ. Want to take look at functional consequences of microbial composition? This might be for you https://msystems.asm.org/content/5/1/e00606-19
🌱🔬The most time-intensive and difficult paper of my PhD is now published in #mSystemsJ! I'm proud of this work and also how much I've grown since I first started this experiment. Read on to learn more about how the functions of bacterial communities impact plant growth and health. 👇
November 25, 2024 at 6:30 PM
🌱🔬The most time-intensive and difficult paper of my PhD is now published in #mSystemsJ! I'm proud of this work and also how much I've grown since I first started this experiment. Read on to learn more about how the functions of bacterial communities impact plant growth and health. 👇
What rules successful lager fermentation in novel hybrids? We developed dozens of hybrids to solve this & evaluated parental contribution, ploidy levels, mitochondria and volatile compounds in beer. Have a look to our latest article in @mSystemsJ journals.asm.org/doi/10.1128/...
Understanding brewing trait inheritance in de novo Lager yeast hybrids | mSystems
Our study investigates the principles of lager yeast hybridization between Saccharomyces cerevisiae and Saccharomyces eubayanus. This process gave rise to the lager yeast Saccharomyces pastorianus. By...
journals.asm.org
November 16, 2024 at 1:51 AM
What rules successful lager fermentation in novel hybrids? We developed dozens of hybrids to solve this & evaluated parental contribution, ploidy levels, mitochondria and volatile compounds in beer. Have a look to our latest article in @mSystemsJ journals.asm.org/doi/10.1128/...
Honored and excited to join the editorial team at @mSystemsJ <a href="http://msystems.asm.org/interior-eic.php#editors" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/interior-eic.php#editors Looking forward to seeing more #openaccess science!
November 13, 2024 at 7:49 PM
Honored and excited to join the editorial team at @mSystemsJ <a href="http://msystems.asm.org/interior-eic.php#editors" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/interior-eic.php#editors Looking forward to seeing more #openaccess science!
Open-Source Sequence Clustering Methods Improve the State Of the Art <a href="http://msystems.asm.org/content/1/1/e00003-15" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/1/1/e00003-15 from @mSystemsJ
November 13, 2024 at 7:15 PM
Open-Source Sequence Clustering Methods Improve the State Of the Art <a href="http://msystems.asm.org/content/1/1/e00003-15" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/1/1/e00003-15 from @mSystemsJ
My 2 cents on the microbiology of the built environment: "The Built Environment Is a Microbial Wasteland" @mSystemsJ <a href="http://msystems.asm.org/content/1/2/e00033-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/1/2/e00033-16
November 13, 2024 at 6:59 PM
My 2 cents on the microbiology of the built environment: "The Built Environment Is a Microbial Wasteland" @mSystemsJ <a href="http://msystems.asm.org/content/1/2/e00033-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/1/2/e00033-16
We got a super nice email from @ASMicrobiology earlier in the year, informing us that @thaasophobia's microbial community-scale metabolic modeling tool, called MICOM, was one of the most highly cited articles in @mSystemsJ last year
https://msystems.asm.org/content/5/1/e00606-19
https://msystems.asm.org/content/5/1/e00606-19
November 13, 2024 at 6:42 PM
We got a super nice email from @ASMicrobiology earlier in the year, informing us that @thaasophobia's microbial community-scale metabolic modeling tool, called MICOM, was one of the most highly cited articles in @mSystemsJ last year
https://msystems.asm.org/content/5/1/e00606-19
https://msystems.asm.org/content/5/1/e00606-19
What Have We Learned about the Microbiomes of Indoor Environments? <a href="http://msystems.asm.org/content/1/4/e00083-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/1/4/e00083-16 by @stephensbrent in @mSystemsJ
November 13, 2024 at 6:26 PM
What Have We Learned about the Microbiomes of Indoor Environments? <a href="http://msystems.asm.org/content/1/4/e00083-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/1/4/e00083-16 by @stephensbrent in @mSystemsJ
What Have We Learned about the Microbiomes of Indoor Environments? <a href="http://msystems.asm.org/content/1/4/e00083-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/1/4/e00083-16 @mSystemsJ @stephensbrent
November 13, 2024 at 6:26 PM
What Have We Learned about the Microbiomes of Indoor Environments? <a href="http://msystems.asm.org/content/1/4/e00083-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/1/4/e00083-16 @mSystemsJ @stephensbrent
Got exciting microbial results to publish? Submit to @ASMicrobiology's new open access @mSystemsJ journal!
November 13, 2024 at 6:26 PM
Got exciting microbial results to publish? Submit to @ASMicrobiology's new open access @mSystemsJ journal!
Microbial Communities Are Well Adapted to Disturbances in Energy Input <a href="http://msystems.asm.org/content/1/5/e00117-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/1/5/e00117-16 @MEPjoe @JulesDeep @mSystemsJ
November 13, 2024 at 6:10 PM
Microbial Communities Are Well Adapted to Disturbances in Energy Input <a href="http://msystems.asm.org/content/1/5/e00117-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/1/5/e00117-16 @MEPjoe @JulesDeep @mSystemsJ
and here's an early career perspective I wrote for @mSystemsJ this year, which lays out some of my thinking on the human gut microbiome & foreshadows some of the work that we plan to publish in 2020 https://msystems.asm.org/content/4/3/e00155-19
November 13, 2024 at 5:53 PM
and here's an early career perspective I wrote for @mSystemsJ this year, which lays out some of my thinking on the human gut microbiome & foreshadows some of the work that we plan to publish in 2020 https://msystems.asm.org/content/4/3/e00155-19
Balance Trees Reveal Microbial Niche Differentiation <a href="http://msystems.asm.org/content/2/1/e00162-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/1/e00162-16 @mSystemsJ @KnightLabNews
November 13, 2024 at 5:37 PM
Balance Trees Reveal Microbial Niche Differentiation <a href="http://msystems.asm.org/content/2/1/e00162-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/1/e00162-16 @mSystemsJ @KnightLabNews
Lifestyle and HGT-Mediated Evolution of M. schaedleri, a Core Member of the Murine Gut Microbiota <a href="http://msystems.asm.org/content/2/1/e00171-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/1/e00171-16 @mSystemsJ
November 13, 2024 at 5:37 PM
Lifestyle and HGT-Mediated Evolution of M. schaedleri, a Core Member of the Murine Gut Microbiota <a href="http://msystems.asm.org/content/2/1/e00171-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/1/e00171-16 @mSystemsJ
Uncovering the Horseshoe Effect in Microbial Analyses <a href="http://msystems.asm.org/content/2/1/e00166-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/1/e00166-16 @mSystemsJ @KnightLabNews
November 13, 2024 at 5:20 PM
Uncovering the Horseshoe Effect in Microbial Analyses <a href="http://msystems.asm.org/content/2/1/e00166-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/1/e00166-16 @mSystemsJ @KnightLabNews
Deblur: a new method for OTU calling. Not based on % clustering & works on very large data sets <a href="http://msystems.asm.org/content/2/2/e00191-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/2/e00191-16 @mSystemsJ
November 13, 2024 at 5:20 PM
Deblur: a new method for OTU calling. Not based on % clustering & works on very large data sets <a href="http://msystems.asm.org/content/2/2/e00191-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/2/e00191-16 @mSystemsJ
Our latest work on how invasive plants impact grassland soils <a href="http://msystems.asm.org/content/2/2/e00178-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/2/e00178-16 @mSystemsJ @gilbertjacka #mpgranch #soilecology
November 13, 2024 at 5:20 PM
Our latest work on how invasive plants impact grassland soils <a href="http://msystems.asm.org/content/2/2/e00178-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/2/e00178-16 @mSystemsJ @gilbertjacka #mpgranch #soilecology
Have cool microbial research that you want to publish in an open-access journal? Consider @mSystemsJ --> I did --> <a href="http://msystems.asm.org/content/2/2/e00178-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/2/e00178-16
November 13, 2024 at 5:20 PM
Have cool microbial research that you want to publish in an open-access journal? Consider @mSystemsJ --> I did --> <a href="http://msystems.asm.org/content/2/2/e00178-16" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/2/e00178-16
Multi-omics Analysis of Periodontal Pocket Microbial Communities Pre- and Post-Treatment <a href="http://msystems.asm.org/content/2/3/e00016-17" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/3/e00016-17 @ScottTKelley @mSystemsJ
November 13, 2024 at 5:03 PM
Multi-omics Analysis of Periodontal Pocket Microbial Communities Pre- and Post-Treatment <a href="http://msystems.asm.org/content/2/3/e00016-17" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/3/e00016-17 @ScottTKelley @mSystemsJ
Excited to see @mSystemsJ partnering with @overleaf to build a custom LaTeX template for easy-submission! <a href="http://msystems.asm.org/content/overleaf-pilot" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/overleaf-pilot
November 13, 2024 at 4:47 PM
Excited to see @mSystemsJ partnering with @overleaf to build a custom LaTeX template for easy-submission! <a href="http://msystems.asm.org/content/overleaf-pilot" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/overleaf-pilot
& just before the end of the year @thaasophobia's work on metagenome-scale metabolic modeling of the human gut microbiome (MICOM) was accepted for publication in @mSystemsJ https://www.biorxiv.org/content/10.1101/361907v3 -- this method enables mapping of ecological structure to...
November 13, 2024 at 4:46 PM
& just before the end of the year @thaasophobia's work on metagenome-scale metabolic modeling of the human gut microbiome (MICOM) was accepted for publication in @mSystemsJ https://www.biorxiv.org/content/10.1101/361907v3 -- this method enables mapping of ecological structure to...
Cool new method from the @ashley17061 lab published in @mSystemsJ
A Synthetic Community System for Probing Microbial Interactions Driven by Exometabolites <a href="http://msystems.asm.org/content/2/6/e00129-17" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/6/e00129-17
A Synthetic Community System for Probing Microbial Interactions Driven by Exometabolites <a href="http://msystems.asm.org/content/2/6/e00129-17" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/6/e00129-17
November 13, 2024 at 4:29 PM
Cool new method from the @ashley17061 lab published in @mSystemsJ
A Synthetic Community System for Probing Microbial Interactions Driven by Exometabolites <a href="http://msystems.asm.org/content/2/6/e00129-17" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/6/e00129-17
A Synthetic Community System for Probing Microbial Interactions Driven by Exometabolites <a href="http://msystems.asm.org/content/2/6/e00129-17" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky">http://msystems.asm.org/content/2/6/e00129-17