Read the full paper: Use of high-affinity scFv antibody NUsc1 for isolation and sensitive detection of Alzheimer’s-associated amyloid beta oligomers

Read the full paper: Isolation and Characterization of a High-Affinity Anti-TSPAN-1 scFv Antibody from a Phage-Display Library for Planarian Neoblast Targeting

Fast antibody and T-cell receptor sequence numbering (IMGT, Kabat, Chothia, Martin, AHo) and FR/CDR segmentation for Python, Polars, Rust, JavaScript and the command line.

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Confira a votação nas eleições 2026 em Itapecuru Mirim (MA) por local de votação para presidente.

Read the full paper: Anti–PD-L1 scFv–Secreting Fifth-Generation GD2-CAR T Cells Enhance Antitumor Function Against GD2-Low Retinoblastoma

Read the full paper: Antibody format matters: A comparative analysis of VHH and scFv domains reveals superior in vivo CAR T cell function with VHH domains

Plasmids were constructed using standard Gibson cloning methods. DNA fragments of APJ, BILF1, TfR1 scFv H7 (ref. 29), TfR1 VHH-A (ref. 30), APJ VHH JN241 (ref. 32), A2AR VH/VL domains Hu3F6 H5-L5 (ref. 33), CCR6 VH/VL domains from 4H11 (ref. 44) and Fc variants (knobs-into-holes Fc for heterobispecific constructs28, G236R/L328R/D356E/L358M and H435A variants to abolish FcγR and FcRn interactions61,62,63 and N297G variant for increased stability and developability64) were synthesized by Integrated DNA Technologies or Twist Bioscience. Binder sequences and/or Fc variants were subcloned into a pFuse-hIgG1 vector (InvivoGen) for mammalian expression. The pcDNA-FLAG–AT1R and pCDNA-FLAG–RXFP1 plasmids were obtained as previously described31,65. AT1R VHH and RXFP1 VHH were developed as previously described31,66. ADORA2A-DuET was a gift from Thomas Sakmar (Addgene plasmid 213174; http://n2t.net/addgene:213174; RRID:Addgene_213174). The pCDH-TfR-mApple lentiviral vector was a gift from Angus Johnston (Addgene plasmid 179384; http://n2t.net/addgene:179384; RRID:Addgene_179384). GPCR sequences were fused with FLAG tag, HA tag and/or GFP and subcloned into pCDH or Tet-On lentiviral vectors for stable dox-inducible expression (Supplementary Fig. 8). The TfR1 sequence was fused with a v5 tag and/or BFP and subcloned into pCDH lentiviral vector for stable overexpression. NFAT-luciferase reporter was a gift from Toren Finkel (Addgene plasmid 10959; http://n2t.net/addgene:10959; RRID:Addgene_10959). The pGloSensor cAMP...

Retinoblastoma resists immune attack in part through PD-L1. Yuti and colleagues show that GD2-CAR T cells engineered to secrete a PD-L1–blocking antibody fragment strip PD-L1 from tumor cells during contact, preventing the checkpoint upregulation that conventional CAR-T cells provoke, and remain active against tumors with low antigen density.

Tumor-infiltrating plasma cells are key responders to immunotherapy. In this issue of Cancer Cell, Meyerhoff et al. show that plasma cells from lung lesions of anti-PD-1-treated patients target citrullinated proteins. Engineering CAR T cells with these plasma cells scFv reveals anti-tumor specificity without adverse effects, identifying therapeutic opportunities.

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CONCLUSIONS: Our findings establish that scFv-dependent modulation of tonic signaling critically governs CAR-T persistence and antitumor efficacy in DMG. By linking CAR design to transcriptional and epigenetic programs, our study provides a principle-based and predictive framework to inform rational CAR engineering and improve therapeutic outcomes.

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder in which soluble amyloid-beta oligomers (AβOs) play a key role in disease onset and progression. This study further explores NUsc1,...