What if transposases are already transcription factors in disguise?
Our new preprint shows that transcriptional regulatory activity is an intrinsic property of transposases, revealing a direct route for their domestication into transcription factors. www.biorxiv.org/content/10.6...
Our new preprint shows that transcriptional regulatory activity is an intrinsic property of transposases, revealing a direct route for their domestication into transcription factors. www.biorxiv.org/content/10.6...
doi.org
August 28, 2026 at 9:13 AM
What if transposases are already transcription factors in disguise?
Our new preprint shows that transcriptional regulatory activity is an intrinsic property of transposases, revealing a direct route for their domestication into transcription factors. www.biorxiv.org/content/10.6...
Our new preprint shows that transcriptional regulatory activity is an intrinsic property of transposases, revealing a direct route for their domestication into transcription factors. www.biorxiv.org/content/10.6...
1/8 🚨 New preprint from the @sternberglab.bsky.social & @martinjinek.bsky.social labs! CRISPR-associated transposases (CASTs) insert large DNA cargoes at precise genomic locations — no double-strand breaks needed.
May 21, 2026 at 9:17 PM
1/8 🚨 New preprint from the @sternberglab.bsky.social & @martinjinek.bsky.social labs! CRISPR-associated transposases (CASTs) insert large DNA cargoes at precise genomic locations — no double-strand breaks needed.
Discovery and protein language model-guided design of hyperactive transposases - @marcguellc.bsky.social @upf.edu @icreacommunity.bsky.social @crg.eu go.nature.com/4gXLGEw
Discovery and protein language model-guided design of hyperactive transposases - Nature Biotechnology
Phylogenetic mining and protein language models uncover improved PiggyBac transposase sequences.
go.nature.com
October 2, 2025 at 2:06 PM
Discovery and protein language model-guided design of hyperactive transposases - @marcguellc.bsky.social @upf.edu @icreacommunity.bsky.social @crg.eu go.nature.com/4gXLGEw
Site-specific, multi-kilobase insertions and over-writes in mammalian cells, without DSBs, HDR, recombinases, integrases, transposases, or active cell cycling? Using fully or predominantly single-stranded DNA donors?
1/15
1/15
April 29, 2026 at 3:46 PM
Site-specific, multi-kilobase insertions and over-writes in mammalian cells, without DSBs, HDR, recombinases, integrases, transposases, or active cell cycling? Using fully or predominantly single-stranded DNA donors?
1/15
1/15
Check out our latest work on the discovery and engineering of PiggyBac transposases! 🧵
Discovery and protein language model-guided design of hyperactive transposases - @marcguellc.bsky.social @upf.edu @icreacommunity.bsky.social @crg.eu go.nature.com/4gXLGEw
Discovery and protein language model-guided design of hyperactive transposases - Nature Biotechnology
Phylogenetic mining and protein language models uncover improved PiggyBac transposase sequences.
go.nature.com
October 2, 2025 at 6:09 PM
Check out our latest work on the discovery and engineering of PiggyBac transposases! 🧵
Phage-based delivery of CRISPR-associated transposases for targeted bacterial editing
"Here, we engineer phage λ with CRISPR-associated transposases to enable flexible bacterial genome manipulation, including in a mixed microbial community context."
www.pnas.org/doi/10.1073/...
"Here, we engineer phage λ with CRISPR-associated transposases to enable flexible bacterial genome manipulation, including in a mixed microbial community context."
www.pnas.org/doi/10.1073/...
July 29, 2025 at 5:26 PM
Phage-based delivery of CRISPR-associated transposases for targeted bacterial editing
"Here, we engineer phage λ with CRISPR-associated transposases to enable flexible bacterial genome manipulation, including in a mixed microbial community context."
www.pnas.org/doi/10.1073/...
"Here, we engineer phage λ with CRISPR-associated transposases to enable flexible bacterial genome manipulation, including in a mixed microbial community context."
www.pnas.org/doi/10.1073/...
Are you expecting a new age of microbiome engineering
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
www.science.org/doi/10.1126/...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
www.science.org/doi/10.1126/...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
Although metagenomic sequencing has revealed a rich microbial biodiversity in the mammalian gut, methods to genetically alter specific species in the microbiome are highly limited. Here, we introduce ...
www.science.org
November 14, 2025 at 8:08 AM
Are you expecting a new age of microbiome engineering
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
www.science.org/doi/10.1126/...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
www.science.org/doi/10.1126/...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases www.science.org/doi/abs/10.1... #jcampubs
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
Although metagenomic sequencing has revealed a rich microbial biodiversity in the mammalian gut, methods to genetically alter specific species in the microbiome are highly limited. Here, we introduce ...
www.science.org
November 13, 2025 at 8:58 PM
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases www.science.org/doi/abs/10.1... #jcampubs
Oh damn. This looks sick "Here, we show that transcriptional regulatory activity is an intrinsic property of transposases (TPases)"
Evolution of transposons as controlling elements https://www.biorxiv.org/content/10.64898/2026.08.24.746577v1
August 28, 2026 at 3:44 AM
Oh damn. This looks sick "Here, we show that transcriptional regulatory activity is an intrinsic property of transposases (TPases)"
I have extended the deadline to apply for this post-doctoral role to work with me on the Biochemistry of Starship Transposon to this Sunday the 5th of January...original deadline set on a holiday set by HR (not me)! edzz.fa.em3.oraclecloud.com/hcmUI/Candid...
Research Fellow - School of Biosciences - 104926 - Grade 7
This post-doctoral role is associated with Dr. McDonald’s UKRI Future Leader Fellowship, and will explore the biochemistry of the Starship transposases. These transposases are the enzymes responsible ...
edzz.fa.em3.oraclecloud.com
January 2, 2025 at 4:48 PM
I have extended the deadline to apply for this post-doctoral role to work with me on the Biochemistry of Starship Transposon to this Sunday the 5th of January...original deadline set on a holiday set by HR (not me)! edzz.fa.em3.oraclecloud.com/hcmUI/Candid...
Really cool new tech to engineer native gut bacteria inside living animals using mobile CRISPR-associated transposases!
Non-engrafting donor E. coli + broad-host-range conjugation system + guide-RNA–directed targeting
www.science.org/doi/10.1126/...
#microsky #microbiomesky 🧪
Non-engrafting donor E. coli + broad-host-range conjugation system + guide-RNA–directed targeting
www.science.org/doi/10.1126/...
#microsky #microbiomesky 🧪
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
Although metagenomic sequencing has revealed a rich microbial biodiversity in the mammalian gut, methods to genetically alter specific species in the microbiome are highly limited. Here, we introduce ...
www.science.org
November 14, 2025 at 5:46 AM
Really cool new tech to engineer native gut bacteria inside living animals using mobile CRISPR-associated transposases!
Non-engrafting donor E. coli + broad-host-range conjugation system + guide-RNA–directed targeting
www.science.org/doi/10.1126/...
#microsky #microbiomesky 🧪
Non-engrafting donor E. coli + broad-host-range conjugation system + guide-RNA–directed targeting
www.science.org/doi/10.1126/...
#microsky #microbiomesky 🧪
Phylogenetic mining and protein language models uncover improved PiggyBac transposase sequences go.nature.com/4gXLGEw
rdcu.be/fzNSK
rdcu.be/fzNSK
Discovery and protein language model-guided design of hyperactive transposases - Nature Biotechnology
Phylogenetic mining and protein language models uncover improved PiggyBac transposase sequences.
go.nature.com
August 14, 2026 at 1:58 AM
Phylogenetic mining and protein language models uncover improved PiggyBac transposase sequences go.nature.com/4gXLGEw
rdcu.be/fzNSK
rdcu.be/fzNSK
#phage #phagesky
Phage-based delivery of CRISPR-associated transposases for targeted bacterial editing | PNAS www.pnas.org/doi/10.1073/...
Phage-based delivery of CRISPR-associated transposases for targeted bacterial editing | PNAS www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
July 29, 2025 at 4:47 AM
#phage #phagesky
Phage-based delivery of CRISPR-associated transposases for targeted bacterial editing | PNAS www.pnas.org/doi/10.1073/...
Phage-based delivery of CRISPR-associated transposases for targeted bacterial editing | PNAS www.pnas.org/doi/10.1073/...
Very excited to share our latest work in Science on metagenomic editing (MetaEdit) of the gut microbiome in vivo & directly modifying unculturable immune-modulatory SFB bug in the small intestine. 🦠🧬🛠️
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
Although metagenomic sequencing has revealed a rich microbial biodiversity in the mammalian gut, methods to genetically alter specific species in the microbiome are highly limited. Here, we introduce ...
www.science.org
November 13, 2025 at 8:53 PM
Very excited to share our latest work in Science on metagenomic editing (MetaEdit) of the gut microbiome in vivo & directly modifying unculturable immune-modulatory SFB bug in the small intestine. 🦠🧬🛠️
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Structural basis of RNA-guided DNA integration by type I CRISPR-associated transposases www.biorxiv.org/content/10.64898/2026.05.18.725949v1 #cryoem
May 19, 2026 at 11:01 AM
Structural basis of RNA-guided DNA integration by type I CRISPR-associated transposases www.biorxiv.org/content/10.64898/2026.05.18.725949v1 #cryoem
Microbiome delivery of CRISPR-associated transposases enables stable genomic integration of multikilobase DNA payloads in gut-associated microbes in vivo. #NBThighlight www.science.org/doi/10.1126/...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
Although metagenomic sequencing has revealed a rich microbial biodiversity in the mammalian gut, methods to genetically alter specific species in the microbiome are highly limited. Here, we introduce ...
www.science.org
November 17, 2025 at 9:36 AM
Microbiome delivery of CRISPR-associated transposases enables stable genomic integration of multikilobase DNA payloads in gut-associated microbes in vivo. #NBThighlight www.science.org/doi/10.1126/...
#microsky #microbiomesky
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases | Science www.science.org/doi/abs/10.1...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases | Science www.science.org/doi/abs/10.1...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
Although metagenomic sequencing has revealed a rich microbial biodiversity in the mammalian gut, methods to genetically alter specific species in the microbiome are highly limited. Here, we introduce ...
www.science.org
November 14, 2025 at 7:49 AM
#microsky #microbiomesky
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases | Science www.science.org/doi/abs/10.1...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases | Science www.science.org/doi/abs/10.1...
FYI: New online! Programmable enzymes for targeted gene insertion
Programmable enzymes for targeted gene insertion
Nature Reviews Genetics, Published online: 07 September 2026; doi:10.1038/s41576-026-01002-9The programmable insertion of large DNA sequences could overcome fundamental limitations of current genome-editing technologies. In this Review, Lampe, King and Sternberg describe recent advances in recombinases, retrotransposons and CRISPR-associated transposases, and discuss how these systems are enabling mutation-agnostic genome modification.
dlvr.it
September 28, 2026 at 10:10 AM
FYI: New online! Programmable enzymes for targeted gene insertion
Great talk from Aaron Vogan @fungage-lab.bsky.social -the exciting story of the discovery of the huge Starship transposon family in the pezizomycotina that can move up to 3% of the pangenome within and between fungal genomes under control of the Captain Kirc transposases.
@ecfg172025.bsky.social
@ecfg172025.bsky.social
March 4, 2025 at 12:19 PM
Great talk from Aaron Vogan @fungage-lab.bsky.social -the exciting story of the discovery of the huge Starship transposon family in the pezizomycotina that can move up to 3% of the pangenome within and between fungal genomes under control of the Captain Kirc transposases.
@ecfg172025.bsky.social
@ecfg172025.bsky.social
1/9 Metagenomics lets us read microbiomes in nature without cultivation, but writing (editing) them in their native context is still a major challenge.
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
Although metagenomic sequencing has revealed a rich microbial biodiversity in the mammalian gut, methods to genetically alter specific species in the microbiome are highly limited. Here, we introduce ...
science.org
November 14, 2025 at 2:25 PM
1/9 Metagenomics lets us read microbiomes in nature without cultivation, but writing (editing) them in their native context is still a major challenge.
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
🎉 Our first paper from the Querques lab is out!
We uncover the molecular checkpoints controlling cut-and-paste transposition by CRISPR-associated transposons.
Congrats to Mateusz & the whole team! 🧬
www.nature.com/articles/s41...
@univie.ac.at
@meduniwien.ac.at
We uncover the molecular checkpoints controlling cut-and-paste transposition by CRISPR-associated transposons.
Congrats to Mateusz & the whole team! 🧬
www.nature.com/articles/s41...
@univie.ac.at
@meduniwien.ac.at
August 25, 2026 at 5:17 PM
🎉 Our first paper from the Querques lab is out!
We uncover the molecular checkpoints controlling cut-and-paste transposition by CRISPR-associated transposons.
Congrats to Mateusz & the whole team! 🧬
www.nature.com/articles/s41...
@univie.ac.at
@meduniwien.ac.at
We uncover the molecular checkpoints controlling cut-and-paste transposition by CRISPR-associated transposons.
Congrats to Mateusz & the whole team! 🧬
www.nature.com/articles/s41...
@univie.ac.at
@meduniwien.ac.at
Minos transgenesis enables robust, stable germline integration of transgenes in Lepidoptera; providing a reliable alternative to piggyBac, whose native transposases can cause transgene remobilization or instability in moths and butterflies:
It's out, Minos transgenesis in the pantry moth by
@donyaniyaz.bsky.social
@lucalivraghi.bsky.social
High efficient, glowing eye and silk gland markers
peerj.com/articles/202...
@peerj.bsky.social
@donyaniyaz.bsky.social
@lucalivraghi.bsky.social
High efficient, glowing eye and silk gland markers
peerj.com/articles/202...
@peerj.bsky.social
November 12, 2025 at 5:12 PM
Minos transgenesis enables robust, stable germline integration of transgenes in Lepidoptera; providing a reliable alternative to piggyBac, whose native transposases can cause transgene remobilization or instability in moths and butterflies:
Targeted genomic editing of human gut #Bacteroides species based on CRISPR-associated transposases.
STIB enables precise, efficient genome insertion in diverse Bacteroides, advancing targeted #microbiome engineering.
www.sciencedirect.com/science/arti... #microbiota #MicroSky
STIB enables precise, efficient genome insertion in diverse Bacteroides, advancing targeted #microbiome engineering.
www.sciencedirect.com/science/arti... #microbiota #MicroSky
July 3, 2026 at 4:48 PM
Targeted genomic editing of human gut #Bacteroides species based on CRISPR-associated transposases.
STIB enables precise, efficient genome insertion in diverse Bacteroides, advancing targeted #microbiome engineering.
www.sciencedirect.com/science/arti... #microbiota #MicroSky
STIB enables precise, efficient genome insertion in diverse Bacteroides, advancing targeted #microbiome engineering.
www.sciencedirect.com/science/arti... #microbiota #MicroSky
Relaxed DNA substrate specificity of transposases involved in programmed genome rearrangement
🤯
#TEsky
www.biorxiv.org/content/10.1...
🤯
#TEsky
www.biorxiv.org/content/10.1...
Relaxed DNA substrate specificity of transposases involved in programmed genome rearrangement
During post-zygotic development, the ciliate Oxytricha trifallax undergoes massive programmed genome rearrangement that involves over 225,000 DNA cleavage and joining events. An Oxytricha family of Tc...
www.biorxiv.org
March 24, 2025 at 9:53 AM
Relaxed DNA substrate specificity of transposases involved in programmed genome rearrangement
🤯
#TEsky
www.biorxiv.org/content/10.1...
🤯
#TEsky
www.biorxiv.org/content/10.1...