Patrick Murphy
@1patrickmurphy.bsky.social
Associate Professor @ UConn Medical School. Endothelial cells, RNA biology, extracellular matrix, leukocyte-endothelial interactions.
And funding from @ahajournals.bsky.social, NIH-NHLBI and the Marlene L. Cohen and Jerome H. Fleisch Chair among other funding sources.
April 22, 2026 at 1:36 PM
And funding from @ahajournals.bsky.social, NIH-NHLBI and the Marlene L. Cohen and Jerome H. Fleisch Chair among other funding sources.
Very proud of the work of Sarah-Anne Nicholas, the first author and a AHA predoctoral awardee, and the excellent team of immunologists (Tony Vella, Beiyan Zhou, Maria Xu) Annabelle Rodriguez-Oquendo and members of our own lab, including Chris Pathoulas @uconnhealth.bsky.social
April 22, 2026 at 1:36 PM
Very proud of the work of Sarah-Anne Nicholas, the first author and a AHA predoctoral awardee, and the excellent team of immunologists (Tony Vella, Beiyan Zhou, Maria Xu) Annabelle Rodriguez-Oquendo and members of our own lab, including Chris Pathoulas @uconnhealth.bsky.social
Thus, endothelial Elavl1 is induced in atherogenesis and allows CD8 T cell persistence in lesions. Since CD8 T cell accumulation and activation is linked to plaque growth and rupture, and a wide range of viral responses and autoimmune conditions, Elavl1 can locally regulate these activities.
April 22, 2026 at 1:36 PM
Thus, endothelial Elavl1 is induced in atherogenesis and allows CD8 T cell persistence in lesions. Since CD8 T cell accumulation and activation is linked to plaque growth and rupture, and a wide range of viral responses and autoimmune conditions, Elavl1 can locally regulate these activities.
In vitro, a novel tri-cell coculture assay (ECs + antigen presenting cells + CD8 T cells) revealed major defects in the survival of CD8 T cells and their persistence.
April 22, 2026 at 1:36 PM
In vitro, a novel tri-cell coculture assay (ECs + antigen presenting cells + CD8 T cells) revealed major defects in the survival of CD8 T cells and their persistence.
Pathways affected in these altered mRNA transcripts are highly enriched for the regulation of T cell interactions and mitochondrial activity. Remarkably, the deletion of Elavl1 from the endothelium nearly ablated the increase in CD8 T cells seen in carotid artery, with less effect on other cells.
April 22, 2026 at 1:36 PM
Pathways affected in these altered mRNA transcripts are highly enriched for the regulation of T cell interactions and mitochondrial activity. Remarkably, the deletion of Elavl1 from the endothelium nearly ablated the increase in CD8 T cells seen in carotid artery, with less effect on other cells.
Elavl1 deletion exhibited a large effect on ribosomal associated mRNA compared to total mRNA in the same system, suggesting specific effects on this translation process.
April 22, 2026 at 1:36 PM
Elavl1 deletion exhibited a large effect on ribosomal associated mRNA compared to total mRNA in the same system, suggesting specific effects on this translation process.
Elavl1 is enriched in the 5'UTR of transcripts at the ribosome (along with Zc3h10, which is known to regulate mRNA translation).
April 22, 2026 at 1:36 PM
Elavl1 is enriched in the 5'UTR of transcripts at the ribosome (along with Zc3h10, which is known to regulate mRNA translation).
Changes in mRNA at translating ribosomes (an important step to protein production) are very different for some mRNA than total mRNA levels.
April 22, 2026 at 1:36 PM
Changes in mRNA at translating ribosomes (an important step to protein production) are very different for some mRNA than total mRNA levels.
They are in good hands - but I can’t agree to root for the 3-peat. Let’s go Red Sox!
March 25, 2026 at 11:18 PM
They are in good hands - but I can’t agree to root for the 3-peat. Let’s go Red Sox!
Finally, and most importantly, the work has been spearheaded though the hard work and unwavering effort of Chris Pathoulas, with help from Amy Kimble in my own lab, and support from NHLBI and NIH, and @americanheart.bsky.social
February 20, 2026 at 3:58 PM
Finally, and most importantly, the work has been spearheaded though the hard work and unwavering effort of Chris Pathoulas, with help from Amy Kimble in my own lab, and support from NHLBI and NIH, and @americanheart.bsky.social
We thank the patients who have donated tissue, our collaborators at UCLA, Harvard/MGH and Duke, and our local collaborators Antoine Menoret and Anthony Vella, who provided expertise in immune cell analysis, and Bing Hao and Yungfeng Li, who aided in antibody conjugations.
February 20, 2026 at 3:58 PM
We thank the patients who have donated tissue, our collaborators at UCLA, Harvard/MGH and Duke, and our local collaborators Antoine Menoret and Anthony Vella, who provided expertise in immune cell analysis, and Bing Hao and Yungfeng Li, who aided in antibody conjugations.