Benjamin Kroeger
@benjaminkroeger.bsky.social
Microscopist | Melbourne, Australia | cell & dev bio, signalling, mechanobio, transcription | he/him
Reposted by Benjamin Kroeger
🚨 New preprint alert! 🚨
Choanoflagellates reorganize their global cytoskeletal architecture within seconds to explore confined microspaces. This is the main PhD work of the amazing @maitefreired.bsky.social www.biorxiv.org/content/10.6...
A tread 🧵⬇️
Choanoflagellates reorganize their global cytoskeletal architecture within seconds to explore confined microspaces. This is the main PhD work of the amazing @maitefreired.bsky.social www.biorxiv.org/content/10.6...
A tread 🧵⬇️
Rapid repurposing of microvillar content drives a flagellate-to-amoeboid switch in the closest relative of animals
Animal cells extensively remodel their cytoskeleton during differentiation and can notably switch between two major motility modes: flagellum-based swimming and actin-based crawling. We previously sho...
www.biorxiv.org
September 2, 2026 at 9:19 PM
🚨 New preprint alert! 🚨
Choanoflagellates reorganize their global cytoskeletal architecture within seconds to explore confined microspaces. This is the main PhD work of the amazing @maitefreired.bsky.social www.biorxiv.org/content/10.6...
A tread 🧵⬇️
Choanoflagellates reorganize their global cytoskeletal architecture within seconds to explore confined microspaces. This is the main PhD work of the amazing @maitefreired.bsky.social www.biorxiv.org/content/10.6...
A tread 🧵⬇️
Reposted by Benjamin Kroeger
🤩 It's time for some invertebrate eye candy! 🤩 Here's a ctenophore (or comb jelly) stained with phalloidin by John Alexander Salazar Hernandez & imaged on an exaSPIM built by Kaelin Wulf of the team of @adamkglaser.bsky.social as part of the FHL Functional Biodiversity course #microscopy
August 20, 2026 at 2:49 PM
🤩 It's time for some invertebrate eye candy! 🤩 Here's a ctenophore (or comb jelly) stained with phalloidin by John Alexander Salazar Hernandez & imaged on an exaSPIM built by Kaelin Wulf of the team of @adamkglaser.bsky.social as part of the FHL Functional Biodiversity course #microscopy
Reposted by Benjamin Kroeger
Update: #webSMLM (V0.11.0), a browser-based single-molecule localisation microscopy analysis tool, includes 2D/3D phasor/MLE fit, background correction, simulations, 3D drift correction, fast temporal median filtering, rendering, spectrally resolved SMLM (!),.. hohlbeinlab.github.io/webSMLM/webS...
August 17, 2026 at 9:24 AM
Update: #webSMLM (V0.11.0), a browser-based single-molecule localisation microscopy analysis tool, includes 2D/3D phasor/MLE fit, background correction, simulations, 3D drift correction, fast temporal median filtering, rendering, spectrally resolved SMLM (!),.. hohlbeinlab.github.io/webSMLM/webS...
Reposted by Benjamin Kroeger
First preprint from the lab 🚨
We asked when and how global chromatin organization emerges during early development 🐟
We asked when and how global chromatin organization emerges during early development 🐟
August 17, 2026 at 3:58 PM
First preprint from the lab 🚨
We asked when and how global chromatin organization emerges during early development 🐟
We asked when and how global chromatin organization emerges during early development 🐟
Reposted by Benjamin Kroeger
1/8 Breathing seems automatic. But it's a finely tuned motor behavior that has to be controlled. In this preprint, I map the circuits that match a fly's respiration to the metabolic demands of flight and mitigate the risk of dehydration. 🧵🪰
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
August 3, 2026 at 4:38 PM
1/8 Breathing seems automatic. But it's a finely tuned motor behavior that has to be controlled. In this preprint, I map the circuits that match a fly's respiration to the metabolic demands of flight and mitigate the risk of dehydration. 🧵🪰
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted by Benjamin Kroeger
🐞🌟 Calling all young insect enthusiasts!
Join us for #YoungEntomologistsDay organised by @amentsoc.bsky.social – a fun-filled day designed to inspire the next generation of insect scientists through activities, expert talks & chances to meet others who share a passion for insects.
Join us for #YoungEntomologistsDay organised by @amentsoc.bsky.social – a fun-filled day designed to inspire the next generation of insect scientists through activities, expert talks & chances to meet others who share a passion for insects.
Young Entomologists' Day 2026 - Royal Entomological Society
Whether you're already keeping records, raising insects or simply curious about the natural world, this is your chance to explore entomology, build new skills and make lasting connections.
buff.ly
July 30, 2026 at 12:01 PM
🐞🌟 Calling all young insect enthusiasts!
Join us for #YoungEntomologistsDay organised by @amentsoc.bsky.social – a fun-filled day designed to inspire the next generation of insect scientists through activities, expert talks & chances to meet others who share a passion for insects.
Join us for #YoungEntomologistsDay organised by @amentsoc.bsky.social – a fun-filled day designed to inspire the next generation of insect scientists through activities, expert talks & chances to meet others who share a passion for insects.
Reposted by Benjamin Kroeger
New paper! 🚀
A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science.
We hope you find it useful! 💚
A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science.
We hope you find it useful! 💚
July 2, 2026 at 2:21 PM
New paper! 🚀
A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science.
We hope you find it useful! 💚
A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science.
We hope you find it useful! 💚
Reposted by Benjamin Kroeger
Annual killifish continue to surprise us: they make neutrophils before almost every other cell type, even before gastrulation. Neutrophils usually arise from the lateral plate mesoderm (forming in cyan), but in killifish they are already patrolling around the embryo in gold. tinyurl.com/killiphil
June 30, 2026 at 4:11 PM
Annual killifish continue to surprise us: they make neutrophils before almost every other cell type, even before gastrulation. Neutrophils usually arise from the lateral plate mesoderm (forming in cyan), but in killifish they are already patrolling around the embryo in gold. tinyurl.com/killiphil
Reposted by Benjamin Kroeger
Delighted to share our latest preprint "NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations"
www.biorxiv.org/content/10.6...
Code and App: github.com/CellMigratio...
www.biorxiv.org/content/10.6...
Code and App: github.com/CellMigratio...
June 30, 2026 at 4:17 PM
Delighted to share our latest preprint "NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations"
www.biorxiv.org/content/10.6...
Code and App: github.com/CellMigratio...
www.biorxiv.org/content/10.6...
Code and App: github.com/CellMigratio...
Reposted by Benjamin Kroeger
My first PhD paper is out in Nucleic Acids Research! We describe "Ab-trapping" - an antibody artifact that distorts assays relying on antibody diffusion (microscopy, CUT&Tag, CUT&RUN). The revisions made the story much stronger. Check it out!
doi.org/10.1093/nar/...
doi.org/10.1093/nar/...
Antibody-trapping presents a widespread pitfall for microscopy and genomics in the nucleus
Abstract. Chromatin has a complex 3D structure and diverse binding proteins that coordinate the genome’s most essential functions. Many microscopy and geno
doi.org
June 30, 2026 at 9:19 AM
My first PhD paper is out in Nucleic Acids Research! We describe "Ab-trapping" - an antibody artifact that distorts assays relying on antibody diffusion (microscopy, CUT&Tag, CUT&RUN). The revisions made the story much stronger. Check it out!
doi.org/10.1093/nar/...
doi.org/10.1093/nar/...
Reposted by Benjamin Kroeger
A ratiometric membrane tension probe based on a non-conducing TRAAK channel bearing a split HaloTag. Compares well with Flipper-TR (and no need for lifetime imaging capacity): www.biorxiv.org/content/10.6...
June 15, 2026 at 9:07 PM
A ratiometric membrane tension probe based on a non-conducing TRAAK channel bearing a split HaloTag. Compares well with Flipper-TR (and no need for lifetime imaging capacity): www.biorxiv.org/content/10.6...
Reposted by Benjamin Kroeger
Topology-dependent FRET efficiency in living cells via N-C swapping of fluorescent protein fusions by Takayuki Nakajima and team:
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
June 7, 2026 at 8:53 PM
Topology-dependent FRET efficiency in living cells via N-C swapping of fluorescent protein fusions by Takayuki Nakajima and team:
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted by Benjamin Kroeger
Happy #FluorescenceFriday.
I feel so lucky to get to work with these weird and curious primary macrophages from 🐸. They exhibit myriad different morphologies and behaviours, including spinning endlessly and stationary, kinda like progenitor cells under compression from 🐟. Also actomyosin is strong!
I feel so lucky to get to work with these weird and curious primary macrophages from 🐸. They exhibit myriad different morphologies and behaviours, including spinning endlessly and stationary, kinda like progenitor cells under compression from 🐟. Also actomyosin is strong!
May 14, 2026 at 11:12 PM
Happy #FluorescenceFriday.
I feel so lucky to get to work with these weird and curious primary macrophages from 🐸. They exhibit myriad different morphologies and behaviours, including spinning endlessly and stationary, kinda like progenitor cells under compression from 🐟. Also actomyosin is strong!
I feel so lucky to get to work with these weird and curious primary macrophages from 🐸. They exhibit myriad different morphologies and behaviours, including spinning endlessly and stationary, kinda like progenitor cells under compression from 🐟. Also actomyosin is strong!
Reposted by Benjamin Kroeger
We are happy to share our new bioRxiv pre-print on A-P axis patterning. A great collaboration with Felix Zhou (UT Southwestern) & the Yeomans group (Physics, Oxford)
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
April 29, 2026 at 12:19 PM
We are happy to share our new bioRxiv pre-print on A-P axis patterning. A great collaboration with Felix Zhou (UT Southwestern) & the Yeomans group (Physics, Oxford)
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted by Benjamin Kroeger
These images show live embryos of animals (jellyfish, crustacean, worm, sea urchin, sea squirt, beetle) and one of animals closest single-celled relatives. They were captured taking advantage of fluorescent proteins localised on the outer membrane of cells, allowing us to observe cell outlines. 1/9
April 19, 2026 at 6:08 PM
These images show live embryos of animals (jellyfish, crustacean, worm, sea urchin, sea squirt, beetle) and one of animals closest single-celled relatives. They were captured taking advantage of fluorescent proteins localised on the outer membrane of cells, allowing us to observe cell outlines. 1/9
Reposted by Benjamin Kroeger
Check out our new release on BioRxiv
co-lead by @pm-mueller.bsky.social
👉 rb.gy/lnrizk
special🙏 to
Severine Kunz #MDC
@leventallab.bsky.social
@andimicroscopy.bsky.social
@ewerslab.bsky.social and Kedar Narayan @NCI CCR VolumeEM
Are the days of discovering new cellular structures over?? /1
co-lead by @pm-mueller.bsky.social
👉 rb.gy/lnrizk
special🙏 to
Severine Kunz #MDC
@leventallab.bsky.social
@andimicroscopy.bsky.social
@ewerslab.bsky.social and Kedar Narayan @NCI CCR VolumeEM
Are the days of discovering new cellular structures over?? /1
April 15, 2026 at 4:52 PM
Check out our new release on BioRxiv
co-lead by @pm-mueller.bsky.social
👉 rb.gy/lnrizk
special🙏 to
Severine Kunz #MDC
@leventallab.bsky.social
@andimicroscopy.bsky.social
@ewerslab.bsky.social and Kedar Narayan @NCI CCR VolumeEM
Are the days of discovering new cellular structures over?? /1
co-lead by @pm-mueller.bsky.social
👉 rb.gy/lnrizk
special🙏 to
Severine Kunz #MDC
@leventallab.bsky.social
@andimicroscopy.bsky.social
@ewerslab.bsky.social and Kedar Narayan @NCI CCR VolumeEM
Are the days of discovering new cellular structures over?? /1
Reposted by Benjamin Kroeger
One of biggest mysteries in biology: how did complex eukaryotic cells evolve from simple microbes? ~1.8 billion years ago, an archaeal cell likely merged with a bacterium to form the first eukaryotic cell, but can we ever find direct evidence of this transformative event? 🦠 🚶♂️
April 12, 2026 at 1:58 PM
One of biggest mysteries in biology: how did complex eukaryotic cells evolve from simple microbes? ~1.8 billion years ago, an archaeal cell likely merged with a bacterium to form the first eukaryotic cell, but can we ever find direct evidence of this transformative event? 🦠 🚶♂️
Reposted by Benjamin Kroeger
In memoriam: Catherine Rabouille (1962–2025) url: rupress.org/jcb/article/...
In memoriam: Catherine Rabouille (1962–2025)
The cell biology community mourns the loss of Catherine Rabouille, an exceptional scientist whose determination, innovation, and fearless engagement with i
rupress.org
April 6, 2026 at 1:41 PM
In memoriam: Catherine Rabouille (1962–2025) url: rupress.org/jcb/article/...
Reposted by Benjamin Kroeger
How do proteins find their way to the cell's leading edge? @jimgalbraith27.bsky.social & @cathygalbraith.bsky.social break down how bleach line, FLOP, single-particle tracking, FCS & iPALM reveal intracellular fluid flow and an actin-myosin compartment barrier. doi.org/10.1038/s414...
April 3, 2026 at 8:17 PM
How do proteins find their way to the cell's leading edge? @jimgalbraith27.bsky.social & @cathygalbraith.bsky.social break down how bleach line, FLOP, single-particle tracking, FCS & iPALM reveal intracellular fluid flow and an actin-myosin compartment barrier. doi.org/10.1038/s414...
Reposted by Benjamin Kroeger
A high-affinity split-HaloTag for live-cell protein labeling. And much more.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
A high-affinity split-HaloTag for live-cell protein labeling
Nature Communications - Lin and colleagues present high-affinity split-HaloTag pairs for protein tagging and multiplexed labelling. This versatile system allows protein visualisation with diverse...
www.nature.com
March 26, 2026 at 3:39 PM
A high-affinity split-HaloTag for live-cell protein labeling. And much more.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Benjamin Kroeger
New piece on the mechanics of squamous epithelial shape transition highlighting the role of tensile forces, force transmission by Dumpy & elastic resistance by the ECM in the developing wing. Terrific work from Stefan Harmansa @morphomechanics.bsky.social with Alex Erlich🍾👏:
tinyurl.com/2p58b3w3
tinyurl.com/2p58b3w3
March 25, 2026 at 10:53 AM
New piece on the mechanics of squamous epithelial shape transition highlighting the role of tensile forces, force transmission by Dumpy & elastic resistance by the ECM in the developing wing. Terrific work from Stefan Harmansa @morphomechanics.bsky.social with Alex Erlich🍾👏:
tinyurl.com/2p58b3w3
tinyurl.com/2p58b3w3
Reposted by Benjamin Kroeger
Expansion microscopy of a loricate choanoflagellate, generated by Mylan Ansel in our lab 🤩
(yes, the lorica expands. No, we don't understand how either)
(yes, the lorica expands. No, we don't understand how either)
March 20, 2026 at 4:24 PM
Expansion microscopy of a loricate choanoflagellate, generated by Mylan Ansel in our lab 🤩
(yes, the lorica expands. No, we don't understand how either)
(yes, the lorica expands. No, we don't understand how either)
Reposted by Benjamin Kroeger
Unstructured transcription factor interactions enable emergent specificity | @science.org www.science.org/doi/10.1126/...
Unstructured transcription factor interactions enable emergent specificity
How intrinsically disordered regions (IDRs) influence chromatin binding and nuclear organization of transcription factors (TFs) remains unclear. We employed proximity-assisted photoactivation (PAPA), ...
www.science.org
March 19, 2026 at 6:51 PM
Unstructured transcription factor interactions enable emergent specificity | @science.org www.science.org/doi/10.1126/...
Reposted by Benjamin Kroeger
I finally had some time to improve my poster about staging Drosophila embryos in collaboration with @stramerlab.bsky.social and we would like to share this with the community! You can find it here or at a higher resolution on my portfolio: www.behance.net/gallery/2458...
#DrosophilaEmbryogenesis
#DrosophilaEmbryogenesis
March 16, 2026 at 1:15 PM
I finally had some time to improve my poster about staging Drosophila embryos in collaboration with @stramerlab.bsky.social and we would like to share this with the community! You can find it here or at a higher resolution on my portfolio: www.behance.net/gallery/2458...
#DrosophilaEmbryogenesis
#DrosophilaEmbryogenesis
Reposted by Benjamin Kroeger
This is why we love #CalciumSignaling
Look how mechanical damage triggers long range Ca2+ waves in this plant !!
By @annalisabellandi.bsky.social, who is now around here ;)
Full www.science.org/doi/10.1126/... @science.org #microscopy #cell #mechanobiology 🧪🔬
Look how mechanical damage triggers long range Ca2+ waves in this plant !!
By @annalisabellandi.bsky.social, who is now around here ;)
Full www.science.org/doi/10.1126/... @science.org #microscopy #cell #mechanobiology 🧪🔬
March 16, 2026 at 8:59 PM
This is why we love #CalciumSignaling
Look how mechanical damage triggers long range Ca2+ waves in this plant !!
By @annalisabellandi.bsky.social, who is now around here ;)
Full www.science.org/doi/10.1126/... @science.org #microscopy #cell #mechanobiology 🧪🔬
Look how mechanical damage triggers long range Ca2+ waves in this plant !!
By @annalisabellandi.bsky.social, who is now around here ;)
Full www.science.org/doi/10.1126/... @science.org #microscopy #cell #mechanobiology 🧪🔬