Doulin Shepherd
@dcshepherd.bsky.social
Post doc in the Ghosal Lab at the University of Melbourne
I study host pathogen interactions using Cryo-ET and STA stuff
#teamtomo
I study host pathogen interactions using Cryo-ET and STA stuff
#teamtomo
Reposted by Doulin Shepherd
When imaging at high mag using cryo-ET, field of view reduces. How to overcome this limit? How do we get a "bird’s-eye view" without compromising resolution? Our very own
@HamishBrown has a solution to this: “montage tomography with a rectangular beam!” www.biorxiv.org/content/10.6...
@HamishBrown has a solution to this: “montage tomography with a rectangular beam!” www.biorxiv.org/content/10.6...
October 7, 2026 at 12:34 PM
When imaging at high mag using cryo-ET, field of view reduces. How to overcome this limit? How do we get a "bird’s-eye view" without compromising resolution? Our very own
@HamishBrown has a solution to this: “montage tomography with a rectangular beam!” www.biorxiv.org/content/10.6...
@HamishBrown has a solution to this: “montage tomography with a rectangular beam!” www.biorxiv.org/content/10.6...
Reposted by Doulin Shepherd
🌀 How do spirochete bacteria swim through thick fluids like champions?
We solved T. denticola flagella structure - asymmetric proteins expand one side, compress the other for perfect corkscrew motion!
@debnathghosal.bsky.social
🔗 doi.org/10.64898/202...
#StructuralBiology #CryoEM #Microbiology
We solved T. denticola flagella structure - asymmetric proteins expand one side, compress the other for perfect corkscrew motion!
@debnathghosal.bsky.social
🔗 doi.org/10.64898/202...
#StructuralBiology #CryoEM #Microbiology
February 5, 2026 at 6:37 AM
🌀 How do spirochete bacteria swim through thick fluids like champions?
We solved T. denticola flagella structure - asymmetric proteins expand one side, compress the other for perfect corkscrew motion!
@debnathghosal.bsky.social
🔗 doi.org/10.64898/202...
#StructuralBiology #CryoEM #Microbiology
We solved T. denticola flagella structure - asymmetric proteins expand one side, compress the other for perfect corkscrew motion!
@debnathghosal.bsky.social
🔗 doi.org/10.64898/202...
#StructuralBiology #CryoEM #Microbiology
Reposted by Doulin Shepherd
📣 New paper alert! This is a story of molecular tricks that let spirochetes (spiral-shaped bacteria) drill through tissue. www.biorxiv.org/content/10.6...
February 5, 2026 at 2:19 PM
📣 New paper alert! This is a story of molecular tricks that let spirochetes (spiral-shaped bacteria) drill through tissue. www.biorxiv.org/content/10.6...
Reposted by Doulin Shepherd
Wonderful spirochete asymmetric flagellar structural work by @lucatroman.bsky.social Grateful to be part of the story supported by @debnathghosal.bsky.social
📣 New paper alert! This is a story of molecular tricks that let spirochetes (spiral-shaped bacteria) drill through tissue. www.biorxiv.org/content/10.6...
February 5, 2026 at 9:21 PM
Wonderful spirochete asymmetric flagellar structural work by @lucatroman.bsky.social Grateful to be part of the story supported by @debnathghosal.bsky.social
Reposted by Doulin Shepherd
📣 New paper alert!
One of the most exciting projects we've done in recent years is now out on BioRxiv: "An Asgard archaeon from a modern analog of ancient microbial mats".
Glimpse into early complex life! ❄️🦠 🔬🧬
www.biorxiv.org/content/10.1.... A thread...
One of the most exciting projects we've done in recent years is now out on BioRxiv: "An Asgard archaeon from a modern analog of ancient microbial mats".
Glimpse into early complex life! ❄️🦠 🔬🧬
www.biorxiv.org/content/10.1.... A thread...
An Asgard archaeon from a modern analog of ancient microbial mats
It has been proposed that eukaryotic cells evolved via symbiosis between sulfate-reducing bacteria and hydrogen-producing archaea. Here we describe a highly enriched culture of a novel Asgard archaeon...
www.biorxiv.org
July 25, 2025 at 1:29 PM
📣 New paper alert!
One of the most exciting projects we've done in recent years is now out on BioRxiv: "An Asgard archaeon from a modern analog of ancient microbial mats".
Glimpse into early complex life! ❄️🦠 🔬🧬
www.biorxiv.org/content/10.1.... A thread...
One of the most exciting projects we've done in recent years is now out on BioRxiv: "An Asgard archaeon from a modern analog of ancient microbial mats".
Glimpse into early complex life! ❄️🦠 🔬🧬
www.biorxiv.org/content/10.1.... A thread...
My total reliance on Nvidia GPUs is a bit concerning.
May 27, 2025 at 11:22 PM
My total reliance on Nvidia GPUs is a bit concerning.
It seems like, no matter what technique you use, it's Fourier transforms all the way down.
November 27, 2024 at 11:48 AM
It seems like, no matter what technique you use, it's Fourier transforms all the way down.
For tomography and STA, I feel like EMAN2 was ahead of its time. I am still confused as to why it isn't used by more people.
November 10, 2024 at 8:32 AM
For tomography and STA, I feel like EMAN2 was ahead of its time. I am still confused as to why it isn't used by more people.