Giang Le
@giangle-1.bsky.social
PhD student at University of Toronto.
Reposted by Giang Le
A couple years in the making, but the preprint is finally out! We developed a pipeline for timelapse, volumetric FLIM (vFLIM) that incorporates multi-channel light sheet excitation, a SPAD array detector for widefield FLIM, and machine learning lifetime processing.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
vFLIM: Machine Learning-enabled Light Sheet Fluorescence Lifetime Imaging
The lifetime of fluorescent molecules provides an orthogonal readout to fluorescence intensity, opening experimental possibilities of measuring changes in local molecular environments, mechanical tens...
www.biorxiv.org
August 27, 2026 at 2:04 PM
A couple years in the making, but the preprint is finally out! We developed a pipeline for timelapse, volumetric FLIM (vFLIM) that incorporates multi-channel light sheet excitation, a SPAD array detector for widefield FLIM, and machine learning lifetime processing.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted by Giang Le
Applications are invited to our programme supporting early career researchers to establish their own independent research groups. Please circulate and further details of how to apply by 25 September are here: www.bioc.cam.ac.uk/research/ind... #Biochemistry #EarlyCareer #Postdocs #Science
August 27, 2026 at 8:48 AM
Applications are invited to our programme supporting early career researchers to establish their own independent research groups. Please circulate and further details of how to apply by 25 September are here: www.bioc.cam.ac.uk/research/ind... #Biochemistry #EarlyCareer #Postdocs #Science
Reposted by Giang Le
New paper from the lab where we successfully image CFP/YFP-based FRET reporters in parallel with a second FRET reporter composed of mRuby2/miRFP670nano3 in the same cell
onlinelibrary.wiley.com/doi/10.1002/...
onlinelibrary.wiley.com/doi/10.1002/...
Red‐to‐Near‐Infrared Fluorescence Resonance Energy Transfer Reporters for Dual Imaging Applications
Genetically encoded FRET biosensors reveal dynamic signaling in living cells, but overlapping spectra have limited the simultaneous monitoring of multiple pathways. We introduce a red-to-near infrare...
onlinelibrary.wiley.com
July 13, 2026 at 10:05 AM
New paper from the lab where we successfully image CFP/YFP-based FRET reporters in parallel with a second FRET reporter composed of mRuby2/miRFP670nano3 in the same cell
onlinelibrary.wiley.com/doi/10.1002/...
onlinelibrary.wiley.com/doi/10.1002/...
Happy to share our newly developed BV-binding optogenetic tool FenixS/Ash1.
Our goal was to make a small, red light-responsive optogenetic core that can function with endogenous level of BV in mammalian cells.
www.biorxiv.org/content/10.1...
Our goal was to make a small, red light-responsive optogenetic core that can function with endogenous level of BV in mammalian cells.
www.biorxiv.org/content/10.1...
www.biorxiv.org
December 1, 2025 at 4:37 AM
Happy to share our newly developed BV-binding optogenetic tool FenixS/Ash1.
Our goal was to make a small, red light-responsive optogenetic core that can function with endogenous level of BV in mammalian cells.
www.biorxiv.org/content/10.1...
Our goal was to make a small, red light-responsive optogenetic core that can function with endogenous level of BV in mammalian cells.
www.biorxiv.org/content/10.1...